Toxicology Reports, Journal Year: 2024, Volume and Issue: unknown, P. 101885 - 101885
Published: Dec. 1, 2024
Language: Английский
Biology of Reproduction, Journal Year: 2024, Volume and Issue: 111(2), P. 242 - 268
Published: May 2, 2024
Abstract The field of Developmental Origins Health and Disease has primarily focused on maternal programming offspring health. However, emerging evidence suggests that paternal factors, including the seminal microbiome, could potentially play important roles in shaping developmental trajectory long-term health outcomes. Historically, microbes present semen were regarded as inherently pathogenic agents. this dogma recently been challenged by discovery a diverse commensal microbial community within healthy males. In addition, recent studies suggest transmission semen-associated into female reproductive tract during mating potentials to not only influence fertility embryo development but also contribute offspring. review, we summarize current knowledge microbiota both humans animals followed discussing their potential involvement We propose discuss mechanisms through which influences are transmitted via microbiome. Overall, review provides insights microbiome-based programing, will expand our understanding currently epigenetic modifications, oxidative stresses, cytokines.
Language: Английский
Citations
9
Stem Cells and Cloning Advances and Applications, Journal Year: 2025, Volume and Issue: Volume 18, P. 15 - 34
Published: Feb. 1, 2025
The mammalian oocyte is pivotal in reproductive biology, acting as a central hub for cellular reprogramming and stemness. It uniquely contributes half of the zygotic nuclear genome entirety mitochondrial genome, ensuring individual development health. Oocyte-mediated reprogramming, exemplified by transfer, resets somatic cell identity to achieve pluripotency has transformative potential regenerative medicine. This process critical understanding differentiation, improving assisted technologies, advancing cloning stem research. During fertilization, maternal-zygotic transition shifts developmental control from maternal factors activation, establishing totipotency. Oocytes also harbor that guide remodeling, epigenetic modifications, metabolic enabling early embryogenesis. Structures like mitochondria, lipid droplets, cytoplasmic lattices contribute energy production, molecular regulation, organization. Recent insights into components, such ooplasmic nanovesicles endolysosomal vesicular assemblies (ELVAS), highlight their roles maintaining homeostasis, protein synthesis, efficiency. By unraveling mechanisms inherent oocytes, we advance our cloning, therapy, highlighting valuable significance biology
Language: Английский
Citations
1
Plant Stress, Journal Year: 2025, Volume and Issue: unknown, P. 100754 - 100754
Published: Jan. 1, 2025
Language: Английский
Citations
0
Food and Chemical Toxicology, Journal Year: 2025, Volume and Issue: 199, P. 115342 - 115342
Published: Feb. 21, 2025
Language: Английский
Citations
0
Published: Feb. 27, 2025
The first cell-fate decision is the process by which cells of an embryo take on distinct lineage identities for time, thus representing beginning developmental patterning. Here, we demonstrate that molecular chaperone heat shock protein A2 (HSPA2), a member 70 kDa (HSP70) family, asymmetrically expressed in late 2-cell stage mouse embryos. knockdown Hspa2 one blastomeres prevented its progeny predominantly toward inner cell mass (ICM) fate. In contrast, overexpression two-cell did not induce to differentiate towards ICM Furthermore, demonstrated HSPA2 interacts with CARM1 and levels correlate ICM-associated genes it CARM1. Collectively, our results identify as critical early regulator mammalian
Language: Английский
Citations
0
eLife, Journal Year: 2025, Volume and Issue: 13
Published: Feb. 28, 2025
The first cell-fate decision is the process by which cells of an embryo take on distinct lineage identities for time, thus representing beginning developmental patterning. Here, we demonstrate that molecular chaperone heat shock protein A2 (HSPA2), a member 70 kDa (HSP70) family, asymmetrically expressed in late 2-cell stage mouse embryos. knockdown Hspa2 one blastomeres prevented its progeny predominantly towards inner cell mass (ICM) fate. In contrast, overexpression did not induce blastomere to differentiate ICM Furthermore, demonstrated HSPA2 interacted with CARM1 and levels correlated ICM-associated genes. Collectively, our results identify as critical early regulator mammalian
Language: Английский
Citations
0
Theriogenology, Journal Year: 2025, Volume and Issue: 240, P. 117387 - 117387
Published: March 12, 2025
Language: Английский
Citations
0
Cell Proliferation, Journal Year: 2025, Volume and Issue: unknown
Published: April 18, 2025
ABSTRACT The mammalian life cycle initiates with the transition of genetic control from maternal to embryonic genome during zygotic activation (ZGA), which becomes pivotal for development. Nevertheless, understanding conservation genes and transcription factors (TFs) that underlie ZGA remains limited. Here, we compiled a comprehensive set mice, humans, pigs, bovines goats, including Nr5a2 TPRX1/2 . identification 111 homologous through comparative analyses was followed by discovery conserved coding region, suggesting potential sequence preferences genes. Notably, an interpretable machine learning model based on k ‐mer core features showed excellent performance in predicting (area under ROC curve [AUC] > 0.81), revealing abundant intricate 6‐base specific patterns binding TFs, motifs NR5A2 TPRX1/2. Further analysis demonstrated gene epigenetic modification play equally important roles regulating Ultimately, developed ZGAExplorer platform provide invaluable resource screening Our study unravels determinants across species multi‐omics data integration learning, yielding insights into regulatory mechanisms developmental arrest.
Language: Английский
Citations
0
Open Biology, Journal Year: 2025, Volume and Issue: 15(3)
Published: March 1, 2025
It is well accepted that sperm carry an RNA cargo with functions in early embryo development. However, most research has focused on the role of small RNAs, such as microRNAs, transfer RNAs and long non-coding while protein-coding messenger (mRNAs) received less attention, even though they represent a substantial amount pool. Here, we curated mouse transcriptomic data from mature selected abundant mRNAs (above 0.7 quantile). The obtained gene list was further filtered using two criteria: (i) are statistically higher one-cell compared to MII oocyte transcriptome, indicative paternal mRNA contribution after fertilization; (ii) found bound ribosomes embryo, positive translation zygote translatome. Our analysis identified 94 genes form networks functionally involved epigenetic chromatin organization, expression, processing during genome activation. These findings underscore significant sperm-borne embryonic development inheritance, highlighting need for fully understand their functions.
Language: Английский
Citations
0
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown
Published: July 19, 2024
Abstract The first cell-fate decision is the process by which cells of an embryo take on distinct lineage identities for time, thus representing beginning developmental patterning. Here, we demonstrate that molecular chaperone heat shock protein A2 (HSPA2), a member 70 kDa (HSP70) family, asymmetrically expressed in late 2-cell stage mouse embryos. knockdown Hspa2 one blastomeres prevented its progeny predominantly toward inner cell mass (ICM) fate. In contrast, overexpression two-cell did not induce to differentiate towards ICM Furthermore, demonstrated HSPA2 interacts with CARM1 and levels correlate ICM-associated genes it CARM1. Collectively, our results identify as critical early regulator mammalian
Language: Английский
Citations
0