H3K4me3 Genome-Wide Distribution and Transcriptional Regulation of Transposable Elements by RNA Pol2 Deposition DOI Open Access

Xiaowei Chen,

Hua Yang, Liqin Wang

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(24), P. 13545 - 13545

Published: Dec. 18, 2024

Zygotic genome activation (ZGA) is critical for early embryo development and meticulously regulated by epigenetic modifications. H3K4me3 a transcription-permissive histone mark preferentially found at promoters, but its distribution across features remains incompletely understood. In this study, we investigated the genome-wide enrichment of during embryonic stem cells (ESCs) in both sheep mice. We discovered that broad domains were present MII stage oocytes progressively diminished, while promoter was increased correlated with gene upregulation ZGA sheep. Additionally, reported dynamic transposable elements (TEs) Specifically, LINE1 ERVL, two subsets TEs, associated their expression Furthermore, TEs greatly following Kdm5b knockdown, RNA polymerase II (Pol2) also markedly knockout ESCs These findings suggest plays important roles regulating TE through interaction Pol2, providing valuable insights into regulation initiation cell fate determination H3K4me3.

Language: Английский

Deciphering the Role of Epigenetics in Plant Pathogen Resistance: Strategies for Sustainable Sugarcane Management DOI Creative Commons

Faisal Mehdi,

Yuan-li Wu,

Shuzhen Zhang

et al.

Plant Stress, Journal Year: 2025, Volume and Issue: unknown, P. 100754 - 100754

Published: Jan. 1, 2025

Language: Английский

Citations

2
Contribution of the seminal microbiome to paternal programming DOI Creative Commons

Justine Kilama,

Carl R Dahlen, Lawrence P. Reynolds

et al.

Biology of Reproduction, Journal Year: 2024, Volume and Issue: 111(2), P. 242 - 268

Published: May 2, 2024

Abstract The field of Developmental Origins Health and Disease has primarily focused on maternal programming offspring health. However, emerging evidence suggests that paternal factors, including the seminal microbiome, could potentially play important roles in shaping developmental trajectory long-term health outcomes. Historically, microbes present semen were regarded as inherently pathogenic agents. this dogma recently been challenged by discovery a diverse commensal microbial community within healthy males. In addition, recent studies suggest transmission semen-associated into female reproductive tract during mating potentials to not only influence fertility embryo development but also contribute offspring. review, we summarize current knowledge microbiota both humans animals followed discussing their potential involvement We propose discuss mechanisms through which influences are transmitted via microbiome. Overall, review provides insights microbiome-based programing, will expand our understanding currently epigenetic modifications, oxidative stresses, cytokines.

Language: Английский

Citations

9
The Mammalian Oocyte: A Central Hub for Cellular Reprogramming and Stemness DOI Creative Commons
Islam M. Saadeldin, Seif Ehab,

Mashan Essa F Alshammari

et al.

Stem Cells and Cloning Advances and Applications, Journal Year: 2025, Volume and Issue: Volume 18, P. 15 - 34

Published: Feb. 1, 2025

The mammalian oocyte is pivotal in reproductive biology, acting as a central hub for cellular reprogramming and stemness. It uniquely contributes half of the zygotic nuclear genome entirety mitochondrial genome, ensuring individual development health. Oocyte-mediated reprogramming, exemplified by transfer, resets somatic cell identity to achieve pluripotency has transformative potential regenerative medicine. This process critical understanding differentiation, improving assisted technologies, advancing cloning stem research. During fertilization, maternal-zygotic transition shifts developmental control from maternal factors activation, establishing totipotency. Oocytes also harbor that guide remodeling, epigenetic modifications, metabolic enabling early embryogenesis. Structures like mitochondria, lipid droplets, cytoplasmic lattices contribute energy production, molecular regulation, organization. Recent insights into components, such ooplasmic nanovesicles endolysosomal vesicular assemblies (ELVAS), highlight their roles maintaining homeostasis, protein synthesis, efficiency. By unraveling mechanisms inherent oocytes, we advance our cloning, therapy, highlighting valuable significance biology

Language: Английский

Citations

1
BHPF inhibits early embryonic development in mice by disrupting maternal-to-zygotic transition and mitochondrial function DOI
Zhi‐Ming Ding,

Hongzhen Ruan,

Yujie Wang

et al.

Food and Chemical Toxicology, Journal Year: 2025, Volume and Issue: 199, P. 115342 - 115342

Published: Feb. 21, 2025

Language: Английский

Citations

0
The asymmetric expression of HSPA2 in blastomeres governs the first embryonic cell-fate decision DOI Open Access
Jiayin Gao, Jiawei Wang, Shiyu Liu

et al.

Published: Feb. 27, 2025

The first cell-fate decision is the process by which cells of an embryo take on distinct lineage identities for time, thus representing beginning developmental patterning. Here, we demonstrate that molecular chaperone heat shock protein A2 (HSPA2), a member 70 kDa (HSP70) family, asymmetrically expressed in late 2-cell stage mouse embryos. knockdown Hspa2 one blastomeres prevented its progeny predominantly toward inner cell mass (ICM) fate. In contrast, overexpression two-cell did not induce to differentiate towards ICM Furthermore, demonstrated HSPA2 interacts with CARM1 and levels correlate ICM-associated genes it CARM1. Collectively, our results identify as critical early regulator mammalian

Language: Английский

Citations

0
The asymmetric expression of HSPA2 in blastomeres governs the first embryonic cell-fate decision DOI Creative Commons
Jiayin Gao, Jiawei Wang,

Shiyu Liu

et al.

eLife, Journal Year: 2025, Volume and Issue: 13

Published: Feb. 28, 2025

The first cell-fate decision is the process by which cells of an embryo take on distinct lineage identities for time, thus representing beginning developmental patterning. Here, we demonstrate that molecular chaperone heat shock protein A2 (HSPA2), a member 70 kDa (HSP70) family, asymmetrically expressed in late 2-cell stage mouse embryos. knockdown Hspa2 one blastomeres prevented its progeny predominantly towards inner cell mass (ICM) fate. In contrast, overexpression did not induce blastomere to differentiate ICM Furthermore, demonstrated HSPA2 interacted with CARM1 and levels correlated ICM-associated genes. Collectively, our results identify as critical early regulator mammalian

Language: Английский

Citations

0
SETD2 regulates oocytes in vitro maturation through histone methylation and maternal mRNA degradation in yak DOI

Hui Zhang,

Yangyang Pan, Meng Wang

et al.

Theriogenology, Journal Year: 2025, Volume and Issue: 240, P. 117387 - 117387

Published: March 12, 2025

Language: Английский

Citations

0
Sperm-borne mRNAs: potential roles in zygote genome activation and epigenetic inheritance DOI Creative Commons
Betina González, Candela R. González

Open Biology, Journal Year: 2025, Volume and Issue: 15(3)

Published: March 1, 2025

It is well accepted that sperm carry an RNA cargo with functions in early embryo development. However, most research has focused on the role of small RNAs, such as microRNAs, transfer RNAs and long non-coding while protein-coding messenger (mRNAs) received less attention, even though they represent a substantial amount pool. Here, we curated mouse transcriptomic data from mature selected abundant mRNAs (above 0.7 quantile). The obtained gene list was further filtered using two criteria: (i) are statistically higher one-cell compared to MII oocyte transcriptome, indicative paternal mRNA contribution after fertilization; (ii) found bound ribosomes embryo, positive translation zygote translatome. Our analysis identified 94 genes form networks functionally involved epigenetic chromatin organization, expression, processing during genome activation. These findings underscore significant sperm-borne embryonic development inheritance, highlighting need for fully understand their functions.

Language: Английский

Citations

0
Deciphering Sequence Determinants of Zygotic Genome Activation Genes: Insights From Machine Learning and the ZGAExplorer Platform DOI Creative Commons

Jixiang Xing,

Siqi Yang,

Yuchao Liang

et al.

Cell Proliferation, Journal Year: 2025, Volume and Issue: unknown

Published: April 18, 2025

ABSTRACT The mammalian life cycle initiates with the transition of genetic control from maternal to embryonic genome during zygotic activation (ZGA), which becomes pivotal for development. Nevertheless, understanding conservation genes and transcription factors (TFs) that underlie ZGA remains limited. Here, we compiled a comprehensive set mice, humans, pigs, bovines goats, including Nr5a2 TPRX1/2 . identification 111 homologous through comparative analyses was followed by discovery conserved coding region, suggesting potential sequence preferences genes. Notably, an interpretable machine learning model based on k ‐mer core features showed excellent performance in predicting (area under ROC curve [AUC] > 0.81), revealing abundant intricate 6‐base specific patterns binding TFs, motifs NR5A2 TPRX1/2. Further analysis demonstrated gene epigenetic modification play equally important roles regulating Ultimately, developed ZGAExplorer platform provide invaluable resource screening Our study unravels determinants across species multi‐omics data integration learning, yielding insights into regulatory mechanisms developmental arrest.

Language: Английский

Citations

0
Melatonin and spermidine protect early mouse embryos from ethylparaben toxicity by restoring histone modifications and subcellular structures DOI
Yongfeng Wang, Y.Y. Wan, Qing Tian

et al.

Ecotoxicology and Environmental Safety, Journal Year: 2025, Volume and Issue: 300, P. 118444 - 118444

Published: June 3, 2025

Language: Английский

Citations

0