Uncovering the significance of CBX3 as an up-and-coming biomarker in cardio-vascular health

Epigenetics Communications, Journal Year: 2025, Volume and Issue: 5(1)

Published: Jan. 7, 2025

Cardiovascular disease (CVD) is the primary cause of mortality globally with a multifactorial etiology that involves epigenetics. Chromobox 3 (CBX3), major isoform heterochromatin protein 1, involved in intricate epigenetic mechanisms affecting congestive heart failure. In patients CVD affected by lung cancer risk, CBX3 exerts sophisticated mechanism action, suppressing proliferation, migration, and formation neointima vascular smooth muscle cells (VSMCs) Notch3 pathway, indicating potential protective function against remodeling atherosclerosis. However, broader im- pact on endothelial function, as well its effects monocyte/macro- phage lymphocyte infiltration within arterial wall, remain poorly understood. Since very little known so far, more definite research would be needed to reveal fine along relationship molecular processes prospects biomarker. Specifically, biological features could examined gain greater insight into risks. This review outlines role associated feasibility for optimizing pre-existing therapy developing new therapeutic strategies based personalized medicine.

Language: Английский

---

Cell Reprogramming, Transdifferentiation, and Dedifferentiation Approaches for Heart Repair

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 3063 - 3063

Published: March 27, 2025

Cardiovascular disease (CVD) remains the leading cause of death globally, with myocardial infarction (MI) being a major contributor. The current therapeutic approaches are limited in effectively regenerating damaged cardiac tissue. Up-to-date strategies for heart regeneration/reconstitution aim at remodeling through repairing tissue an external cell source or by stimulating existing cells to proliferate and repopulate compromised area. Cell reprogramming is addressed this challenge as promising solution, converting fibroblasts other types into functional cardiomyocytes, either reverting pluripotent state directly switching lineage. Several such gene editing application miRNA small molecules have been explored their potential enhance regeneration. Those take advantage plasticity introducing factors that regress maturity vitro, allowing later differentiation thus endorsing transplantation, promote situ proliferation, leveraged scaffolds embedded pro-regenerative promoting efficient restoration. Despite notable advancements, important challenges persist, including low efficiency, maturation limitations, safety concerns clinical applications. Nonetheless, integrating these innovative offers alternative restoring function reducing dependency on full transplants.

Language: Английский

---

Serum expression of microRNA-21, microRNA-125a, microRNA-125b, microRNA-214 in coronary artery disease patients

Russian Cardiology Bulletin, Journal Year: 2025, Volume and Issue: 20(2), P. 13 - 13

Published: May 22, 2025

Background. Coronary artery disease (CAD) is determined by interaction of environmental factors with epigenetic and genetic factors. MicroRNA-21, microRNA-125a, microRNA-125b microRNA-214 are small non-coding RNAs the most important regulators gene expression. -125a, -125b -214 insufficiently studied in CAD patients. Objective. To explore serum expression microRNA-21, microRNA-125b, patients different features clinical course. Material methods. The study included 129 (men 56.6% women 43.4%) aged 63.17±8.57 years. comparison group consisted 45 people without 55.6% 44.4%) 62.09±8.79 years (p>0.05). Serum microRNA was analyzed using real-time polymerase chain reaction. Relative estimated accordance standard 2-ΔCt procedure. Results. higher (p<0.001). MicroRNA-21 >16.43 units increases risk 3.49 times (HR 3.49, 95% CI 1.70—7.17, p<0.001), microRNA-125a >7.21 — 5.18 5.18, 2.29—11.69, >3.49 4.03 4.03, 1.86—8.72, p=0.0001), >2.47 3.99 3.99, 1.91—8.37, p<0.001). Expression microRNA-21 early than older (p<0.05). myocardial infarction (MI) MI Conclusion. group, as well MI.

Language: Английский

---

Exosomal miRNA Changes Associated with Restoration to Sinus Rhythm in Atrial Fibrillation Patients

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(7), P. 3861 - 3861

Published: March 29, 2024

We aimed to identify serum exosomal microRNAs (miRNAs) associated with the transition from atrial fibrillation (AF) sinus rhythm (SR) and investigate their potential as biomarkers for early recurrence of AF within three months post-treatment. collected blood samples eight patients at Chang Gung Memorial Hospital in Taiwan both immediately before 14 days following control treatment. Exosomes were isolated these samples, small RNA sequencing was performed. Using DESeq2 analysis, we identified nine miRNAs (16-2-3p, 22-3p, 23a-3p, 23b-3p, 125a-5p, 328-3p, 423-5p, 504-5p, 582-3p) restoration SR. Further analysis using DIABLO model revealed a correlation between decreased expression miR-125a-5p miR-328-3p AF. Furthermore, is longer duration AF, presumably indicating more extensive state underlying cardiac remodeling. In addition, reads mapped mRNA sequences, leading identification mRNAs (AC005041.1, ARHGEF12, AMT, ANO8, BCL11A, DIO3OS, EIF4ENIF1, G2E3-AS1, HERC3, LARS, NT5E, PITX1, SLC16A12, ZBTB21) Monitoring miRNA patterns may be beneficial optimizing treatment outcomes patients.

Language: Английский

---

Candidate Signature miRNAs from Secreted miRNAome of Human Lung Microvascular Endothelial Cells in Response to Different Oxygen Conditions: A Pilot Study

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(16), P. 8798 - 8798

Published: Aug. 13, 2024

Oxygen conditions in the lung determine downstream organ functionality by setting partial pressure of oxygen, regulating redox homeostasis and activating mediators that can be propagated blood stream. Examples for such are secreted soluble or vesicle-bound molecules (proteins nucleic acids) taken up remote target cells impacting their metabolism signaling pathways. MicroRNAs (miRNAs) have gained significant interest as intercellular communicators, biomarkers therapeutic targets this context. Due to high stability stream, they also been attributed a role "memory molecules" able modulate gene expression upon repeated (stress) exposures. In study, we aimed identify quantify released miRNAs from microvascular endothelial response different oxygen conditions. We combined next-generation sequencing (NGS) cellular mRNA with bioinformatic analyses order delineate molecular events on extracellular level putative interdependence. show identified miRNA networks potential co-mediate some events, observed context hypoxia, hyperoxia, intermittent hypoxia hypoxia/hyperoxia.

Language: Английский

---

Role of NHERF1 in MicroRNA Landscape Changes in Aging Mouse Kidneys

Biomolecules, Journal Year: 2024, Volume and Issue: 14(9), P. 1048 - 1048

Published: Aug. 23, 2024

MicroRNAs (miRNAs) play important roles in the regulation of cellular function and fate via post-transcriptional gene expression. Although several miRNAs are associated with physiological processes kidney diseases, not much is known about changes aging kidneys. We previously demonstrated that sodium hydrogen exchanger 1 (NHERF1) expression regulates responses to cisplatin, age-dependent salt-sensitive hypertension, sodium-phosphate cotransporter trafficking. However, mechanisms driving these regulatory effects NHERF1 on unknown. Here, we hypothesize dysregulation miRNA-mediated networks induce fibrosis cytokines may depend To address this hypothesis, compared miRNA kidneys from both male female old (12-18-month-old) young (4-7-month-old) wild-type (WT) knockout (NHERF1

Language: Английский

---

Global research landscape on the genetics of congenital heart disease: A bibliometric and visualized analysis via VOSviewer and CiteSpace

Medicine, Journal Year: 2024, Volume and Issue: 103(43), P. e40261 - e40261

Published: Oct. 25, 2024

Genetic factors play a significant role in the development of congenital heart disease (CHD). Many studies on genetics CHD have been published worldwide; however, no research has assessed and mapped global landscape these studies. This bibliometric visualized study aimed to delineate hotspots trends field genetics. Scientific papers from January 1, 1950, December 31, 2023, were obtained by searching Web Science Core Collection. The metadata each chosen paper extracted, analyzed, using tools such as Microsoft Excel 2021, VOSviewer, CiteSpace. final analysis included 5317 discussing CHD. countries journals that highest number United States (n = 2118), American Journal Medical Genetics Part A 332), respectively. In addition genetics, keywords tetralogy Fallot, ventricular septal defect, atrial defect appeared most frequently among 8365 keywords. Eight clusters formed categorize Keywords case–control study, whole genome sequencing, exome sequencing 6, 7, 8, respectively, had latest average publication year all clusters. To best our knowledge, this is first Tetralogy are topics. interactions between environmental genetic pathogenesis CHD, etiology CHD-associated pulmonary arterial hypertension, molecular via high-throughput genomic technology possible areas future

Language: Английский

---

Binding-driven forward tearing protospacer activated CRISPR-Cas12a system and applications for microRNA detection

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Nov. 8, 2024

CRISPR-Cas12a system, characterized by its precise sequence recognition and cleavage activity, has emerged as a powerful programmable tool for molecular diagnostics. However, current CRISPR-Cas12a-based nucleic acid detection methods, particularly microRNA (miRNA) detection, necessitate additional bio-engineering strategies to exert control over Cas12a activity. Herein, we propose an engineered target-responsive hairpin DNA activator (TRHDA) mediate forward tearing protospacer activated which enables direct miRNA with high specificity sensitivity. Target specifically binding can drive in the stem of structure, facilitating complementarity between crRNA spacer activate Cas12a. Upon input-responsive Cas12a, universal biosensing method multiple miRNAs (miR-21, let-7a, miR-30a) also exceptional capability identifying single-base mismatches distinguishing homologous let-7/miR-30 family members. Besides, TRHDA-mediated Cas12a-powered realized evaluation miR-21 expression levels diverse cellular contexts intracellular imaging. Considering easy programmability responsive region, this strategy could expand other target molecules (e.g., proteins, micromolecules, peptides, exosomes), offers significant implications biomarkers diagnostics utilizing system toolbox.

Language: Английский

---

The Potential Contribution of MyomiRs miR-133a-3p, -133b, and -206 Dysregulation in Cardiovascular Disease Risk

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(23), P. 12772 - 12772

Published: Nov. 27, 2024

Cardiovascular disease (CVD) is a major global health concern. The number of people with CVD expected to rise due aging populations and increasing risk factors such as obesity diabetes. Identifying new molecular markers crucial for early diagnosis treatment. Among these, plasma levels some miRNAs, specifically expressed in cardiac skeletal muscle, known myomiRs, have gained attention their roles cardiovascular health. This study analyzed the miR-133a-3p, -133b, -206 pathogenesis diseases. Using case-control design patients recruited from several nursing homes Calabria (southern Italy) characterized by different types compared non-CVD controls, we found downregulation miR-133a-3p heart failure miR-133b stroke, along overall decreased expression miR-206 patients, although they showed low specificity biomarkers (as based on ROC analysis). In silico functional characterization targets signaling pathways revealed involvement critical processes. Although further research necessary fully elucidate mechanisms clinical utility, findings reported here may provide insight into potential contribution myomiRs injury framework, also offering indications directions.

Language: Английский

---

Exosomes and miRNAs in Cardiovascular Diseases and Transcatheter Pulmonary Valve Replacement: Advancements, Gaps and Perspectives

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(24), P. 13686 - 13686

Published: Dec. 21, 2024

As an important carrier of intercellular information transmission, exosomes regulate the physiological and pathological state local or distant cells by carrying a variety signal molecules such as microRNAs (miRNAs). Current research indicates that miRNAs can serve biomarkers therapeutic targets for cardiovascular diseases (CVDs). This narrative review summarizes progress their in CVDs, particularly pulmonary valve (PVDs), and, first time, explores potential associations with transcatheter replacement (TPVR). Currently, play crucial role determining optimal timing TPVR intervention, they demonstrate broad application prospects post-TPVR right ventricular (RV) remodeling, treatment, prognosis monitoring. However, association between development PVDs, regurgitation, remains unclear. The molecular mechanisms PVDs RV remodeling after have not been fully elucidated, postoperative treatment following is still its infancy. Future must focus on advancing fundamental studies, validating biomarkers, enhancing clinical applications to achieve significant breakthroughs.

Language: Английский

---