Sensitive Detection of Biomarker in Gingival Crevicular Fluid Based on Enhanced Electrochemiluminescence by Nanochannel-Confined Co3O4 Nanocatalyst

Biosensors, Journal Year: 2025, Volume and Issue: 15(1), P. 63 - 63

Published: Jan. 19, 2025

The sensitive detection of inflammatory biomarkers in gingival crevicular fluid (GCF) is highly desirable for the evaluation periodontal disease. Luminol-based electrochemiluminescence (ECL) immunosensors offer a promising approach fast and convenient biomarkers. However, luminol’s low ECL efficiency under neutral conditions remains challenge. This study developed an immunosensor by engineering immunorecognition interface on outer surface mesoporous silica nanochannel film (SNF) confining Co3O4 nanocatalyst within SNF nanochannels to improve luminol efficiency. was grown indium tin oxide (ITO) electrode using simple Stöber solution growth method. A successfully confined through situ electrodeposition, confirmed X-ray photoelectron spectroscopy (XPS) electrochemical measurements. demonstrated excellent electrocatalytic activity, effectively enhancing H2O2 oxidation boosting signal conditions. Using interleukin-6 (IL-6) as proof-of-concept demonstration, epoxy functionalization enabled covalent immobilization capture antibodies, forming specific interface. IL-6 binding induced immunocomplex formation, which reduced allowed quantitative detection. showed linear range from 1 fg mL−1 10 ng mL−1, with limit (LOD) 0.64 mL−1. It also good selectivity anti-interference capabilities, enabling successful artificial GCF samples.

Language: Английский

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Association between dental floss use and atherosclerotic cardiovascular disease in American adults

Heart & Lung, Journal Year: 2025, Volume and Issue: 71, P. 32 - 38

Published: Feb. 21, 2025

Language: Английский

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Salivary IL-1β, IL-6, and IL-10 Are Key Biomarkers of Periodontitis Severity

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(15), P. 8401 - 8401

Published: Aug. 1, 2024

To explore severity and progression biomarkers, we examined the clinical relevance of multiple cytokines mediators involved in inflammatory response periodontitis. A cohort 68 patients was enrolled study periodontal status assessed by current classification diseases. Immune present saliva, both healthy controls, were quantified using a Legendplex-13 panel. Clinic parameters significantly higher PD compared with HC, strong significant association disease (stage) (

Language: Английский

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Clinical Efficacy of Extracellular Vesicle Therapy in Periodontitis: Reduced Inflammation and Enhanced Regeneration

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(11), P. 5753 - 5753

Published: May 25, 2024

Periodontitis, a prevalent inflammatory condition, affects the supporting structures of teeth, leading to significant oral health challenges. Traditional treatments have primarily focused on mechanical debridement, antimicrobial therapy, and surgery, which often fail restore lost periodontal structures. Emerging as novel approach in regenerative medicine, extracellular vesicle (EV) including exosomes, leverages nano-sized vesicles known for facilitating intercellular communication modulating physiological pathological processes. This study is proof-of-concept type that evaluates clinical efficacy EV therapy non-surgical treatment stage I–III periodontitis, focusing its anti-inflammatory potential. The research involved seven patients undergoing healthy individuals. Clinical parameters, plaque index, bleeding probing, probing depth, attachment level, were assessed alongside cytokine levels gingival crevicular fluid. found improvements marked reduction pro-inflammatory cytokines post-treatment, matching subjects, underscoring therapy’s ability not only attenuate inflammation enhance tissue regeneration, but also highlighting potential restoring health. investigation illuminates promising role treatment, advocating shift towards therapies halt disease progression promote structural functional restoration tissues.

Language: Английский

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Comparative Analysis of Gene Expression in Periodontal Ligament Stem Cells Exposed to Biodentine and Bio-C Repair: Implications for Cementogenesis—An In Vitro Study

Oral, Journal Year: 2025, Volume and Issue: 5(1), P. 19 - 19

Published: March 13, 2025

Background/Objectives: Bioactive materials are gaining increased popularity as of choice for pulpal regeneration. A similar trend is emerging with root repair materials; however, there a significant gap in the literature about cementogenic ability bioceramic on periodontal ligament cells. The aim present study was to investigate effect (Biodentine and Bio-C Repair) cementogenesis potential stem cells (PDLSCs). Methods: PDLSCs were isolated using enzymatic digestion approach from sound extracted teeth. Material extracts prepared rubber discs immersed fresh growth medium 24 h at 37 °C. Reverse transcription–quantitative polymerase chain reaction (RT-qPCR) used detect mRNA expression levels markers cementum protein 1 (CEMP1), Cementum attachment (CAP), pathway transforming factor β1(TGF-β1), bone morphogenic 2 (BMP2), inflammatory marker IL-6. Results: Both showed significantly higher gene expressions when compared control groups. Repair Biodentine, except TGF-β1 expression, where both exhibited results. Conclusions: demonstrated Biodentine under tested conditions. Further vivo studies deemed necessary translate findings this into clinical practice.

Language: Английский

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An Overview of the Etiopathogenic Mechanisms Involved in the Expression of the Oral Microbiota

Clinics and Practice, Journal Year: 2025, Volume and Issue: 15(4), P. 80 - 80

Published: April 11, 2025

Background/Objectives: The diversity of the oral microbiota exerts its effects in maintaining dental and overall health. unique genetic profile each individual influences composition microbiota, determining susceptibility to certain diseases. aim is observe role by highlighting pathogenic mechanisms involved dysbiosis identify determinism's influence balance. Methods: This study was designed as a narrative review utilizing some principles guidelines systematic increase methodological rigor. We examined 121 articles such reviews, meta-analyses, editorials, observational studies, which met inclusion exclusion criteria. criteria for studies were follows: (1) that evaluated impact or/and systemic diseases; (2) observed microbiota; (3) interaction with immune system (4); implications microbiota. Results: Host genes regulate inflammatory immunological reactions play microbiological explains increased resistance diseases, including gingivitis or periodontitis. Also, are reflected not only locally, but also generally, being associated various conditions. Conclusions: Understanding determinants may help create individualized therapies preventing managing disorders. A healthy lifestyle adequate hygiene can facilitate diverse balanced microbiome, crucial

Language: Английский

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Relationship Between MCP‐1 Levels in GCF and Periodontitis: A Systematic Review With Meta‐Analysis and Analysis of Molecular Interactions

Journal of Cellular and Molecular Medicine, Journal Year: 2025, Volume and Issue: 29(9)

Published: May 1, 2025

ABSTRACT Monocyte chemoattractant protein 1 (MCP‐1) is involved in monocyte chemotaxis, endothelial activation and regulation of leukocyte function biological activities that promote inflammation, such as periodontitis. A systematic review meta‐analysis was conducted with the primary objective investigating roles MCP‐1 gingival crevicular fluid (GCF) subjects chronic periodontitis compared to periodontally healthy subjects. The study protocol adhered PRISMA guidelines. Digital searches were carried out across several databases, including PubMed, Dentistry & Oral Science Source, ScienceDirect, Scopus, Web Google Scholar. quality studies evaluated using JBI tool for cross‐sectional clinical trials. To assess concentration GCF exposure group versus control group, a employing random‐effects model. search strategy yielded 1694 articles, 14 meeting inclusion criteria 10 articles subjected quantitative analysis. total 497 examined, comprising 298 cases 199 controls. indicated significant increase levels individuals (GCF: SMD = 20.29, 95% CI: 10.33–30.25, Z 3.992, p 0.001*). are elevated controls, suggesting its potential future use diagnostic settings.

Language: Английский

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Oxidative stress in periodontitis and the application of antioxidants in treatment: a narrative review

Frontiers in Physiology, Journal Year: 2025, Volume and Issue: 16

Published: May 13, 2025

Periodontitis has turned into a general oral disease defined by chronic inflammation of the gums and helping tissues teeth. It dramatically influences both systemic health is main trigger tooth loss. tightly linked to oxidative stress, evidence reveals utilization certain antioxidants in related treatments. Our review delves literature periodontitis, centering on latest therapeutic strategies mechanistic evidences. This starts depicting epidemiological features pathogenic mechanisms highlighting vital mechanism microbial community modefications, host immune responses, genetic factors development. Existing treatment approaches for periodontitis involve mechanical cleaning, antibiotic therapy, surgical interventions, pharmacological treatments, with comprehensive exploration strengths limitations each strategy their clinical utilizations. Besides, this investigates emerging therapies, like anti-inflammatory modulation biotechnological control periodontitis. particularly stresses protective effects researches potential slowing inflammatory responses boosting tissue repair. Ultimately, our intends concept individual therapy future directions, comprising precision medicine design utilization.

Language: Английский

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Cardamonin inhibits the expression of inflammatory mediators in TNF-α-stimulated human periodontal ligament cells

Immunopharmacology and Immunotoxicology, Journal Year: 2024, Volume and Issue: 46(4), P. 521 - 528

Published: June 26, 2024

Periodontis is a chronic inflammatory disease induced by periodontopathogenic bacteria. The excessive immune response caused persistent bacterial infection leads to alveolar bone resorption and ultimately tooth loss. Cardamonin biologically active substance that found in the Zingiberaceae family, such as Alpinia zerumbet, classified natural chalcone. There have been no attempts use cardamonin for treatment of periodontitis, reports examined effects on periodontal tissue component cells. aim this study was analyze expression inflammation mediators produced TNFα-stimulated human ligament cells (HPDLCs), including its signal transduction molecules.

Language: Английский

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Severe Periodontitis is Associated With Recurrent Cardiovascular Events—A 10‐Year Longitudinal Cohort Study

Journal of Periodontal Research, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 22, 2024

ABSTRACT Aim The study aimed to elucidate a putative association between severe periodontitis and the incidence of recurrent cardiovascular events in patients with disease (CVD) within 10 years after their initial hospitalisation. Methods A cohort 1002 stationary angiographically proven CVD was included. They were examined regarding prevalence (≥ 30% teeth proximal attachment loss ≥ 5 mm), probing depth, clinical loss, bleeding on probing, number missing oral care habits. Recurrent summarised as combined end point (myocardial infarction, stroke/transitory ischemic attack, death caused by stroke). Survival analyses carried out 10‐year follow‐up period. Hazard ratios (HRs) adjusted for known cardiac risk factors using Cox regression. Results completed 792 patients. overall 42.8%. Severe associated (adjusted hazard ratio [HR] = 1.26, 95% confidence interval [CI] 1.0–1.58 Standard error [SE] 0.11), whereas both, tooth brushing more than once day HR 0.74, CI 0.57–0.97, SE 0.13) performing interdental hygiene 0.71, 0.52–0.99, 0.16) decreased this risk. Conclusions is factor events. Trial Registration ClinicalTrials.gov identifier: NCT01045070

Language: Английский

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