Food-Derived Micronutrients as Alleviators of Age-Related Dysfunction: A Dive into Their Effects and Cellular Mechanisms

Indian Journal of Clinical Biochemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 13, 2025

Language: Английский

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Involvement of Oxidative Stress and Antioxidants in Modification of Cardiac Dysfunction Due to Ischemia–Reperfusion Injury

Antioxidants, Journal Year: 2025, Volume and Issue: 14(3), P. 340 - 340

Published: March 14, 2025

Delayed reperfusion of the ischemic heart (I/R) is known to impair recovery cardiac function and produce a wide variety myocardial defects, including ultrastructural damage, metabolic alterations, subcellular Ca2+-handling abnormalities, activation proteases, changes in gene expression. Although I/R injury has been reported induce formation reactive oxygen species (ROS), inflammation, intracellular Ca2+ overload, generation oxidative stress considered play critical role development dysfunction. Increases production superoxide, hydroxyl radicals, oxidants, such as hydrogen peroxide hypochlorous acid, occur hearts subjected injury. In fact, mitochondria are major source excessive ROS due impairment electron transport system well xanthine oxidase NADPH oxidase. Nitric oxide synthase, mainly present endothelium, also activated injury, leading nitric oxide, which, upon combination with superoxide generates nitrosative stress. Alterations function, sarcolemma, sarcoplasmic reticulum activities, mitochondrial phosphorylation, protease simulated exposing oxyradical-generating (xanthine plus oxidase) or H2O2. On other hand, endogenous antioxidants dismutase, catalase, glutathione peroxidase, concentration transcription factor (Nrf2), which modulates expression various antioxidants, depressed hearts. Furthermore, pretreatment catalase N-acetylcysteine, mercaptopropionylglycerine observed attenuate I/R-induced handling Ca2+-regulatory activities; additionally, it found depress improve function. These observations indicate that intimately involved pathological effects different alterations Thus, we faced task developing safe effective agents for upregulating therapy

Language: Английский

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Thinner Induced Multiple Organ Toxicities in Wistar Rats: Ameliorative Role of Vitamin E (Alpha-Tocopherol)

Turkish Journal of Science and Technology, Journal Year: 2025, Volume and Issue: 20(1), P. 339 - 354

Published: March 9, 2025

Thinner, a common industrial solvent, is popular choice for recreational self-intoxication, leading to potential serious health consequences. This study delves into the acute toxicity of thinner, focusing on its impact various organ systems and ameliorative ability Vitamin E as an antioxidant. Four experimental groups Wistar rats (n-12) were established follow: control, only, thinner only + E. The subjected 3week inhalation protocol, parameters such body weight, biochemical markers (aspartate aminotransferase (AST), alanine (ALT), alkaline phosphatase (ALP), total bilirubin (TBIL), blood urea nitrogen (BUN) creatinine levels), oxidative stress (malondialdehyde, nitrites, glutathione, catalase superoxide dismutase), histopathological examinations assessed using standard procedures. results revealed significant reduction in weight thinner-exposed group. However, group exposed both exhibited marked increase weight. Biochemical (AST, ALT, ALP, TBIL, BUN creatinine) significantly elevated (p

Language: Английский

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Fabrication of Thymoquinone and Ascorbic Acid-Loaded Spanlastics Gel for Hyperpigmentation: In Vitro Release, Cytotoxicity, and Skin Permeation Studies

Pharmaceutics, Journal Year: 2025, Volume and Issue: 17(1), P. 48 - 48

Published: Jan. 2, 2025

Background/Objectives: The demand for a safe compound hyperpigmentation is continuously increasing. Bioactive compounds such as thymoquinone (TQ) and ascorbic acid (AA) induce inhibition of melanogenesis with high safety profile. aim this study was to design evaluate spanlastics gel loaded bioactive agents, TQ AA, the management hyperpigmentation. Methods: Several formulations were successfully fabricated characterized in terms morphology, vesicle size, zeta potential, release. Results: optimized TQ-loaded spanlastic formulation showed an average size 223.40 ± 3.50 nm, 133.00 2.80 nm AA-loaded formulation. highest entrapment efficiency (EE%) 97.18 2.02% 93.08 1.95%, respectively. Additionally, edge activator concentration had significant effect (p < 0.05) on EE%; it found that by increasing amount EA, EE% increases. Following that, optimal fomulation AA incorporated into explored appearance, pH, spreadability, stability, rheology, vitro release, ex vivo permeation study, MTT cytotoxicity. formulated (R-1) has pH 5.53. R-1 significantly more spreadable than control gel, exhibited shear thinning behavior. Most importantly, skin deposition studies confirmed superior from gels. results indicated tyrosinase primarily due TQ. When comparing alone TQ-AA combination, ranged 18.35 42.73% 24.28 42.53%, Both combination concentration-dependent tyrosinase. Conclusions: Spanlastic might represent promising carrier dermal delivery conditions.

Language: Английский

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Transcription Factor Blimp-1: A Central Regulator of Oxidative Stress and Metabolic Reprogramming in Chronic Inflammatory Diseases

Antioxidants, Journal Year: 2025, Volume and Issue: 14(2), P. 183 - 183

Published: Feb. 4, 2025

B-lymphocyte-induced maturation protein 1 (Blimp-1) is a transcription factor that, among other functions, modulates metabolism and helps to regulate antioxidant pathways, which important in the context of chronic inflammatory diseases like diabetes, cardiovascular disease, autoimmune disease. In immune cell function, Blimp-1 has modulatory role orchestration metabolic reprogramming as promoter anti-inflammatory cytokines, including IL-10, responsible for modulating oxidative stress homeostasis. Moreover, also key aspects, such glycolysis fatty acid oxidation, reactive oxygen species levels, well tissue protection. This review depicts an regulator defenses anti-inflammation suggests that could serve therapeutic target dysregulation conditions. The modulation diabetic coronary heart disease atherosclerosis alleviate stress, augment protection tissues, improve outcomes. potential development new treatments these conditions lies synergy between regulation therapies, are future directions may be pursued. emphasizes Blimp-1's emerging importance novel pathogenesis diseases, providing opportunities intervention.

Language: Английский

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Neuroprotective effects of vitamin C on hypoxic-ischemic brain injury in neonatal mice

Pediatric Research, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 17, 2025

Language: Английский

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Efficacy of antioxidant intervention and exercise intervention for lipid peroxidation in dialysis patients: a meta-analysis

Frontiers in Medicine, Journal Year: 2025, Volume and Issue: 12

Published: March 17, 2025

Lipid peroxidation is a major factor known to contribute occurrence of cardiovascular events in dialysis patients. This study aims investigate whether antioxidant interventions can improve lipid damage A comprehensive search PubMed, Embase, and the Cochrane Library was conducted identify eligible randomized controlled trials (RCTs) up June 2024. Endpoints interest included biomarkers related peroxidation. The results from studies were performed using RevMan 5.3 Stata17.0 software. total 25 RCTs included, involving eight such as vitamin C supplementation, E E-coated dialyzer, ω-fatty acid curcumin pomegranate juice exercise intervention, multiple interventions. Outcome indicators malondialdehyde (MDA) oxidized low-density lipoprotein (Ox-LDL). meta-analysis revealed that supplementation caused significant reductions MDA (p = 0.01). Treatment with dialyzer markedly decreased levels < 0.0001). Curcumin significantly reduced Ox-LDL 0.03). Exercise intervention Multiple Supplementation E, treatment, effectively reduce level https://www.crd.york.ac.uk/PROSPERO (CRD42023455399).

Language: Английский

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Effects of reactive oxygen species and antioxidant strategies on wound healing in diabetes

Deleted Journal, Journal Year: 2025, Volume and Issue: unknown

Published: March 18, 2025

Abstract With the rising global prevalence of diabetes, rate chronic wounds associated with this disease is also escalating. Although mechanisms underlying trend are not fully understood, accumulating evidence suggests a critical role for reactive oxygen species (ROS). Increased blood glucose levels, bacterial infections, and impaired energy utilization lead to an imbalance between oxidative antioxidant responses, resulting in ROS accumulation. The cellular system can manage small amount ROS; however, excessive exogenous acts upon cell membrane or even penetrates cell, consequently affecting its activity. Excessive cause enhanced expression inflammatory factors, proliferation, vascular vessel damage, extracellular matrix remodeling disturbance, ultimately hindering diabetic wound healing. regulation during healing intricate involves diverse throughout entire wound‐healing process. This review provides systematic overview relationship signaling pathways along most recent strategies targeting these pathways. aim was outline effective mitigating stress provide novel insights into future research.

Language: Английский

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Erectile Dysfunction and Oxidative Stress: A Narrative Review

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 3073 - 3073

Published: March 27, 2025

Erectile dysfunction (ED) is a prevalent condition affecting male sexual health, characterized by the inability to achieve or maintain satisfactory erections. ED has multifactorial pathogenesis in which psychological, hormonal, neurologic, cardiovascular, and lifestyle factors all contribute progressive decline of erectile function. A critical underlying mechanism involves oxidative stress (OS), an imbalance between reactive oxygen species (ROS) production antioxidant defenses, disrupts endothelial function, reduces nitric oxide (NO) bioavailability, contributes vascular dysfunction. This narrative review explores interplay OS ED, focusing on roles ROS sources such as NADPH oxidase, xanthine uncoupled synthase, mitochondrial It examines impact chronic conditions like hypertension, diabetes mellitus, hyperlipidemia, hypogonadism, smoking obesity, exacerbate through systemic effects. Emerging research underscores potential therapies interventions restore redox balance, improve mitigate ED's progression. also highlights gaps understanding molecular pathways linking emphasizing need for further develop targeted therapeutic strategies.

Language: Английский

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Dysregulated complement activation during acute myocardial infarction leads to endothelial glycocalyx degradation and endothelial dysfunction via the C5a:C5a-Receptor1 axis

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: July 11, 2024

Complement-mediated damage to the myocardium during acute myocardial infarction (AMI), particularly late components of terminal pathway (C5-convertase and C5b-9), have previously been characterized. Unfortunately, only few studies reported a direct association between dysregulated complement activation endothelial function. Hence, little attention has paid role anaphylatoxin C5a. The glycocalyx (eGC) together with cellular actin cortex provide vasoprotective barrier against chronic vascular inflammation. Changes in their nanomechanical properties (stiffness height) are recognized as hallmarks dysfunction they correlate bioavailability vasoactive substances, such nitric oxide (NO). Here, we determined how C5a:C5aR1 axis affects eGC function AMI. Samples fifty-five patients ST-elevation (STEMI) vs. healthy controls were analyzed this study. C5a levels via ELISA; NO quantified chemiluminescence-based. Endothelial cells stimulated or patient sera (with/without C5a-receptor1 antagonist "PMX53") using atomic force microscopy (AFM)-based nanoindentation technique. To measure cytoskeletal tension regulator (RhoA Rac1) G-LISA assays applied. Vascular inflammation was examined by quantifying monocyte-endothelium interaction AFM-based single-cell-force spectroscopy. Serum concentrations significantly increased STEMI. solely stimulation decreased height stiffness, indicating shedding eGC. enhanced RhoA activation, resulting cortical stiffness subsequent reduction concentrations. Monocyte adhesion endothelium after both STEMI serum. degradation- RhoA-induced stiffening attenuated administering PMX53. This study demonstrates that AMI results reduced bioavailability, progressively developing could be prevented antagonizing C5aR1, highlighting C5a:C5a-Receptor1 development AMI, offering new therapeutic approaches for future investigations.

Language: Английский

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