CD133 and CD166 Stem Cells Markers Expression, Clinicopathological Parameters, and Fragmentation Response Patterns of ypT3 Rectal Cancer Following Neoadjuvant Chemoradiotherapy DOI Creative Commons
Diana Lavinia Pricope,

Adriana Grigoraș,

Gabriel Mihail Dimofte

et al.

Biomedicines, Journal Year: 2025, Volume and Issue: 13(6), P. 1300 - 1300

Published: May 26, 2025

Background: The effectiveness of neoadjuvant chemoradiotherapy (nCRT) is variable in locally advanced rectal cancer (LARC) patients, the ypT3 stage having a minimal or moderate response. aim our study was evaluation association between CD133 (Prominin1) and CD166 (ALCAM) expression, survival parameters, clinicopathological characteristics subgroup LARC patients who achieved ypT3, showing post-nCRT TME tumor fragmentation response assessment these CSCs biomarkers value as indicators nCRT Methods: Our group comprised 60 status exhibited pattern following nCRT. Clinicopathological parameter evaluations, along with immunohistochemistry scoring, were performed associations different parameters tested. Results: High expression significantly associated ypN category (p = 0.018), lymphovascular invasion (LVI) 0.009), perineural (PnI) 0.006), grading 0.047), while high LVI 0.020) PnI 0.028). Tumors expressions decreased overall (OS) 0.004 p 0.006). Cox regression analysis identified independent factors reduced (HR 3.237, 0.014 HR 2.866, 0.020). Conclusions: results support hypothesis that are putative CSC aggressive behavior poor prognosis LARC, offering opportunities for personalized targeted therapies.

Language: Английский

Mechanisms and Strategies to Overcome Drug Resistance in Colorectal Cancer DOI Open Access

Jennifer Haynes,

Prasath Manogaran

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(5), P. 1988 - 1988

Published: Feb. 25, 2025

Colorectal cancer (CRC) is a major cause of cancer-related mortality worldwide, with significant impact on public health. Current treatment options include surgery, chemotherapy, radiotherapy, molecular-targeted therapy, and immunotherapy. Despite advancements in these therapeutic modalities, resistance remains challenge, often leading to failure, poor progression-free survival, recurrence. Mechanisms CRC are multifaceted, involving genetic mutations, epigenetic alterations, tumor heterogeneity, the microenvironment. Understanding mechanisms at molecular level crucial for identifying novel targets developing strategies overcome resistance. This review provides an overview diverse driving drug sporadic discusses currently under investigation counteract this Several promising being explored, including targeting transport, key signaling pathways, DNA damage response, cell death modifications, stem cells, The integration emerging approaches that target aims enhance efficacy current treatments improve patient outcomes.

Language: Английский

Citations

2
Computational Elucidation of Hub Genes and Pathways Correlated with the Development of 5-Fluorouracil Resistance in HCT 116 Colorectal Carcinoma Cell Line DOI
Chun Hoe Tan, Siew‐Huah Lim, Kae Shin Sim

et al.

Biochemical Genetics, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 30, 2025

Language: Английский

Citations

1
Innovative Therapeutic Approach Targeting Colon Cancer Stem Cells: Transitional Cold Atmospheric Plasma DOI Creative Commons
Abolfazl Soulat, Taghi Mohsenpour, Leila Roshangar

et al.

ACS Omega, Journal Year: 2025, Volume and Issue: 10(12), P. 12109 - 12121

Published: March 6, 2025

Transitional cold atmospheric plasma (TCAP) represents a novel technique for generating remotely from primary source. It consists of partially nonthermal ionized gas mixture containing charged and neutral particles, photons, free radicals. In recent years, TCAP has attracted considerable attention in biomedical applications. order to evaluate colon cancer stem cells' (CCSCs) proliferation, apoptotic induction, inflammatory response, survival, was utilized both directly indirectly this study. Using argon helium gases, continuously delivered two stages during the experiment. For direct state, irradiated onto CCSCs 3 5 min. indirect technique, Matrigel treated with min before introduction cells. vitro assays demonstrated that exposure significantly reduced viability CCSCs; application had greater impacts than argon. Numerous investigations confirmed induction apoptosis, showing groups more cells altered cellular structures controls (****p < 0.0001). A substantial increase Bax/Bcl-2 ratio found by analyzing expression Bax Bcl-2 genes, indicating increased susceptibility apoptosis (*p = 0.0177 ***p 0.0004). The higher efficacy mode further highlighted marker analysis, which showed significant reduction interleukin-6 interleukin-8 TCAP-helium compared TCAP-argon (**p 0.0015 0.0007). Lastly, proliferation test, relies on Ki-67 expression, noteworthy decline all TCAP-treated groups, group exhibiting most robust impact 0.0014). Overall, findings highlight potential TCAP, particularly helium, as promising approach selectively targeting providing insights into its therapeutic mechanisms treatment. therefore, emerges unique strategy applications cell-targeted therapies.

Language: Английский

Citations

1
Targeting mitochondrial ribosomal protein expression by andrographolide and melatonin for colon cancer treatment. DOI

Advaitha Midde,

Navpreet Arri,

Tibor Kristián

et al.

Cancer Letters, Journal Year: 2025, Volume and Issue: unknown, P. 217647 - 217647

Published: March 1, 2025

Language: Английский

Citations

1
Biomarkers in Colorectal Cancer: Actual and Future Perspectives DOI Open Access
Horia-Dan Lișcu, Nicolae Verga, Dimitrie-Ionuț Atasiei

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(21), P. 11535 - 11535

Published: Oct. 27, 2024

Biomarkers in colorectal cancer (CRC) are of great interest the current literature due to improvements techniques such as liquid biopsy and next-generation sequencing (NGS). However, screening methods vary globally, with multi-target stool DNA (mt-sDNA) predominantly used USA and, more recently, Cologuard Plus; biomarkers Galectins family septins show promise early detection. Gut microbiome assessments, Fusobacterium nucleatum, under intense exploration. Diagnostic tests, circulating analysis via NGS, exhibit effectiveness being increasingly adopted. Circulating tumor cells emerge potential alternatives traditional terms diagnosis prognosis. Predictive well established guidelines; nonetheless, aid machine learning artificial intelligence, these may be improved. This review critically explores actual dynamic landscape CRC future, promising involved screening, diagnosis,

Language: Английский

Citations

5
Construction of a Controlled Drug Delivery and Optical Monitoring System for Colorectal Cancer via Natural Fiber Modification DOI

Quan Cheng,

Hongxia Fu,

Yong Mao

et al.

Journal of Medical and Biological Engineering, Journal Year: 2025, Volume and Issue: unknown

Published: March 28, 2025

Language: Английский

Citations

0
Evaluation of Key Risk Factors Associated with Postoperative Complications in Colorectal Cancer Surgery DOI Creative Commons

Silviu Stefan Marginean,

Mihai Zurzu, Dragoş Garofil

et al.

Journal of Mind and Medical Sciences, Journal Year: 2025, Volume and Issue: 12(1), P. 22 - 22

Published: April 17, 2025

Background: Colorectal surgery remains a cornerstone in the management of colorectal cancer, yet postoperative complications continue to impact surgical outcomes. This study investigates key risk factors influencing morbidity, focusing on patient comorbidities, tumor characteristics, techniques, and anastomotic methods. Methods: A retrospective analysis was conducted 195 patients who underwent cancer between January 2021 December 2024 at Clinical Hospital Nephrology “Carol Davila”. Variables analyzed included demographics, staging, approach, complications. Statistical methods chi-square tests multivariate logistic regression (significance threshold: p < 0.05). Results: The overall complication rate 21%, with leakage observed 8.2% cases. Significant for morbidity cardiovascular disease (p = 0.001), chronic respiratory failure 0.003), renal 0.002). Laparoscopic had lower (7.1%) than open (28%) 0.003). Mechanical anastomosis showed rates manual suturing 0.009). Left-sided resections were associated higher right-sided procedures 0.013). Conclusions: Optimizing outcomes requires personalized perioperative strategies. approaches mechanical significantly reduce Further multicenter studies are needed confirm these findings enhance guidelines.

Language: Английский

Citations

0
Therapeutic potential of stem cells in colorectal cancer management: Current trends and future prospects DOI
M. K. Desai, Namrata B. Parmar, Isha Shah

et al.

Developmental Dynamics, Journal Year: 2025, Volume and Issue: unknown

Published: May 13, 2025

Abstract Colorectal cancer (CRC) ranks among the leading causes of cancer‐related morbidity and mortality worldwide. Despite progress in understanding its molecular intricacies, management CRC, especially advanced stages, remains a significant clinical hurdle. This review delves into evolving landscape stem cell‐based therapeutic strategies with specific focus on interplay between cells (CSCs) CRC pathogenesis treatment resistance. Highlighting pivotal roles CSCs tumor initiation, progression, metastasis, recurrence, comprehensively examines their involvement ranging from normal colonic tissue to initiation. The potential for medicinal purposes is explored, encompassing diverse modalities such as transplantation, differentiation therapy, immunotherapy, gene/cell‐based approaches. Challenges opportunities associated these are also evaluated, providing insights limitations. appraises preclinical investigations contributing cells. Current trials, patient stratification strategies, regulatory considerations related therapies scrutinized. Furthermore, explores emerging trends future directions, including developments cell technologies ethical considerations. It highlights transformative CRC.

Language: Английский

Citations

0
CD133 and CD166 Stem Cells Markers Expression, Clinicopathological Parameters, and Fragmentation Response Patterns of ypT3 Rectal Cancer Following Neoadjuvant Chemoradiotherapy DOI Creative Commons
Diana Lavinia Pricope,

Adriana Grigoraș,

Gabriel Mihail Dimofte

et al.

Biomedicines, Journal Year: 2025, Volume and Issue: 13(6), P. 1300 - 1300

Published: May 26, 2025

Background: The effectiveness of neoadjuvant chemoradiotherapy (nCRT) is variable in locally advanced rectal cancer (LARC) patients, the ypT3 stage having a minimal or moderate response. aim our study was evaluation association between CD133 (Prominin1) and CD166 (ALCAM) expression, survival parameters, clinicopathological characteristics subgroup LARC patients who achieved ypT3, showing post-nCRT TME tumor fragmentation response assessment these CSCs biomarkers value as indicators nCRT Methods: Our group comprised 60 status exhibited pattern following nCRT. Clinicopathological parameter evaluations, along with immunohistochemistry scoring, were performed associations different parameters tested. Results: High expression significantly associated ypN category (p = 0.018), lymphovascular invasion (LVI) 0.009), perineural (PnI) 0.006), grading 0.047), while high LVI 0.020) PnI 0.028). Tumors expressions decreased overall (OS) 0.004 p 0.006). Cox regression analysis identified independent factors reduced (HR 3.237, 0.014 HR 2.866, 0.020). Conclusions: results support hypothesis that are putative CSC aggressive behavior poor prognosis LARC, offering opportunities for personalized targeted therapies.

Language: Английский

Citations

0