SARS-CoV-2 infection of human pluripotent stem cell-derived vascular cells reveals smooth muscle cells as key mediators of vascular pathology during infection DOI Open Access

Alexsia Richards,

Andrew Khalil, Max Friesen

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 7, 2023

Abstract Although respiratory symptoms are the most prevalent disease manifestation of infection by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), nearly 20% hospitalized patients at risk for thromboembolic events. This prothrombotic state is considered a key factor in increased stroke, which observed clinically during both acute and long after clear. Here we develop model SARS-CoV-2 using human-induced pluripotent stem cell-derived endothelial cells (ECs), pericytes (PCs), smooth muscle (SMCs) to recapitulate vascular pathology associated with exposure. Our results demonstrate that perivascular cells, particularly SMCs, susceptible target infection. Utilizing RNA sequencing, characterize transcriptomic changes accompanying PCs, ECs. We observe infected SMCs shift pro-inflammatory increase expression mediators coagulation cascade. Further, show human ECs exposed secretome produce hemostatic factors contribute dysfunction, despite not being direct The findings here observations from patient sera COVID-19 provide mechanistic insight into unique implications cellular level.

Language: Английский

Association of OAS1 gene polymorphism with the severity of COVID‑19 infection DOI Open Access
Noha G. Bader El Din,

Rehab I. Moustafa,

Enaya Ghaleb

et al.

World Academy of Sciences Journal, Journal Year: 2024, Volume and Issue: 6(6)

Published: Oct. 9, 2024

The ongoing coronavirus disease 2019 (COVID‑19) pandemic has underscored the critical need to investigate host genetic factors that may influence susceptibility and severity. Among these, 2'‑5'‑oligoadenylate synthase 1 (OAS1) single nucleotide polymorphism (SNP) rs10774671 been implicated in antiviral response against coronaviruses clinical outcomes. aim of present retrospective study was association between OAS1 gene severity COVID‑19. A total 200 subjects were enrolled, including 75 healthy controls 125 patients with SNP assessed using PCR‑RFLP analysis. findings revealed an upward trend prevalence allele among individuals, who developed a severe course Specifically, 17.8% infection carried GG genotype, 52.8% had GA genotype 30.4% AA (P=0.0001). Notably, exclusively detected COVID‑19 (P<0.0001). Moreover, frequency markedly higher than G Multivariate analysis individuals 6.8‑fold greater likelihood progressing more (odds ratio, 6.86; 95% CI, 2.83‑16.63; P<0.0001). Thus, holds promise as potential marker could be valuable predicting progression outcome infection.

Language: Английский

Citations

1
OAS1 induces endothelial dysfunction and promotes monocyte adhesion through the NFκB pathway in Atherosclerosis DOI
Miao Liang, Weikang Li,

Xixi Xie

et al.

Archives of Biochemistry and Biophysics, Journal Year: 2024, Volume and Issue: 763, P. 110222 - 110222

Published: Nov. 19, 2024

Language: Английский

Citations

1
Evaluation of Suppressor of Cytokine Signaling 1, Interleukin-29 and Lysosomal Trafficking Regulator in Severe COVID-19 DOI Open Access
Mojdeh Lashkari, Ashraf Kariminik, Mohammad Javad Soltani Banavandi

et al.

Journal of Kermanshah University of Medical Sciences, Journal Year: 2024, Volume and Issue: 28(4)

Published: Nov. 13, 2024

Background: The pathogenesis of Severe SARS-CoV-2 is closely linked to severe immune responses and inflammation caused by the virus. In this context, suppressor cytokine signaling 1 (SOCS1) has a crucial role in inhibiting cytokine-induced responses. On other hand, interleukin-29 (IL-29) lysosomal trafficking regulator (LYST) are important molecules involved inducing Objectives: This study aimed assess mRNA levels SOCS1, IL-29, LYST SARS-CoV-2-infected patients with symptoms. Methods: cross-sectional study, 70 infected symptoms healthy controls were evaluated. RNA was extracted from peripheral blood after cDNA synthesis, assessed Real-Time PCR technique Results: revealed that COVID-19 exhibited significant increase IL-29 compared individuals. However, there no observed alterations SOCS1 patient group. Conclusions: results emphasize importance as potential biomarker or therapeutic target for cases. Further research needed investigate specific mechanisms through which influences contributes development disease. Additionally, exploring factors may regulate expression could provide more comprehensive understanding their roles pathogenesis.

Language: Английский

Citations

0
SARS-CoV-2 infection of human pluripotent stem cell-derived vascular cells reveals smooth muscle cells as key mediators of vascular pathology during infection DOI Creative Commons

Alexsia Richards,

Andrew Khalil, Max Friesen

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Dec. 30, 2024

Although respiratory symptoms are the most prevalent disease manifestation of infection by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), nearly 20% hospitalized patients at risk for thromboembolic events. This prothrombotic state is considered a key factor in increased stroke, which observed clinically during both acute and long after clear. Here, we develop model SARS-CoV-2 using human-induced pluripotent stem cell-derived endothelial cells (ECs), pericytes (PCs), smooth muscle (SMCs) to recapitulate vascular pathology associated with exposure. Our results demonstrate that perivascular cells, particularly SMCs, susceptible target infection. Utilizing RNA sequencing, characterize transcriptomic changes accompanying PCs, ECs. We observe infected SMCs shift pro-inflammatory increase expression mediators coagulation cascade. Further, show human ECs exposed secretome produce hemostatic factors contribute dysfunction despite not being direct The findings here observations from patient sera COVID-19 provide mechanistic insight into unique implications cellular level.

Language: Английский

Citations

0
SARS-CoV-2 infection of human pluripotent stem cell-derived vascular cells reveals smooth muscle cells as key mediators of vascular pathology during infection DOI Open Access

Alexsia Richards,

Andrew Khalil, Max Friesen

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 7, 2023

Abstract Although respiratory symptoms are the most prevalent disease manifestation of infection by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), nearly 20% hospitalized patients at risk for thromboembolic events. This prothrombotic state is considered a key factor in increased stroke, which observed clinically during both acute and long after clear. Here we develop model SARS-CoV-2 using human-induced pluripotent stem cell-derived endothelial cells (ECs), pericytes (PCs), smooth muscle (SMCs) to recapitulate vascular pathology associated with exposure. Our results demonstrate that perivascular cells, particularly SMCs, susceptible target infection. Utilizing RNA sequencing, characterize transcriptomic changes accompanying PCs, ECs. We observe infected SMCs shift pro-inflammatory increase expression mediators coagulation cascade. Further, show human ECs exposed secretome produce hemostatic factors contribute dysfunction, despite not being direct The findings here observations from patient sera COVID-19 provide mechanistic insight into unique implications cellular level.

Language: Английский

Citations

1