Antiviral activity of rosemary extract against Zika virus in human dermal fibroblasts
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 31, 2025
Abstract
Flaviviruses
have
increasingly
emerged
and
re-emerged
in
recent
decades,
infecting
millions
of
people
annually.
Zika
virus
is
particularly
concerning
due
to
its
associated
pathological
complications,
including
microcephaly
newborns
Guillain-Barré
syndrome
adults,
posing
a
significant
threat
public
health.
Despite
efforts
made
by
the
scientific
community,
no
licensed
drugs
against
flaviviruses
been
developed.
Medicinal
plants
show
promise
as
novel
source
antiviral
agents,
they
possess
diverse
array
biologically
active
secondary
metabolites,
making
them
potential
candidates
for
therapeutic
use.
We
sought
investigate
rosemary
extract
(RE)
ZIKV
human
dermal
fibroblasts
(HFF-1
cells),
one
earliest
targets
infection.
was
treated
with
various
concentrations
RE
or
individual
major
polyphenols,
rosmarinic
acid
(RA),
carnosic
(CA),
carnosol
(CO),
remaining
infectivity
each
sample
measured
plaque
reduction
assay.
To
evaluate
impact
on
different
stages
replication
cycle,
HFF-1
cells
were
before,
during,
after
infection,
before
exerted
potent
activity
both
Vero
directly
acting
particles
Importantly,
significantly
inhibited
later
cycle
interfering
post-entry
mechanisms
within
host
cell.
Moreover,
RE-derived
polyphenols
CA
CO,
but
not
RA,
shown
reduce
infectivity.
Overall,
impairs
infection
vitro
interacting
prior
adsorption
processes
cycle.
This
study
provides
rigorous
evidence
indicating
development
an
agent,
warranting
further
investigation
into
underlying
inhibitory
effects
other
medically
important
flaviviruses.
Language: Английский
Diterpenes of Salvia (2016–2024): phytochemistry and pharmacology
Phytochemistry Reviews,
Journal Year:
2025,
Volume and Issue:
unknown
Published: April 11, 2025
Language: Английский
Polyphenols Investigation and Antioxidant and Anticholinesterase Activities of Rosmarinus officinalis L. Species from Southwest Romania Flora
Ludovic Everard Bejenaru,
No information about this author
Andrei Biţă,
No information about this author
George Dan Mogoşanu
No information about this author
et al.
Molecules,
Journal Year:
2024,
Volume and Issue:
29(18), P. 4438 - 4438
Published: Sept. 18, 2024
Rosemary
is
one
of
the
most
important
medicinal
plants
for
natural
therapy
due
to
its
multiple
pharmacological
properties,
such
as
antioxidant,
anti-inflammatory,
neuroprotective,
antiproliferative,
antitumor,
hepato-
and
nephroprotective,
hypolipidemic,
hypocholesterolemic,
antihypertensive,
anti-ischemic,
hypoglycemic,
radioprotective,
antimicrobial,
antiviral,
antiallergic,
wound
healing
properties.
Our
study
reports
first
time,
over
a
12-month
period,
identification
quantification
polyphenols
investigation
antioxidant
acetylcholinesterase
(AChE)
inhibitory
activities
Rosmarinus
officinalis
L.
species
harvested
at
flowering
from
flora
southwestern
Romania
(Oltenia
Region).
Identification
polyphenolic
acids
was
made
by
ultra-high-performance
liquid
chromatography/mass
spectrometry
(UHPLC/MS).
Total
phenolic
content
determined
using
spectrophotometric
method.
In
situ
anticholinesterase
were
evaluated
2,2-diphenyl-1-picrylhydrazyl
(DPPH)
AChE
assay,
respectively,
on
high-performance
thin-layer
chromatography
(HPTLC)
plates.
DPPH
radical
scavenging
activity
also
assessed
spectrophotometrically.
The
results
revealed
significant
correlations
between
specific
compounds
measured
biological
activities,
understanding
role
seasonal
variations
providing
insights
into
optimal
harvesting
times
benefits
rosemary.
research
brings
new
information
phytochemical
profile
R.
source
with
Language: Английский
Betulonic Acid Inhibits Type-2 Porcine Reproductive and Respiratory Syndrome Virus Replication by Downregulating Cellular ATP Production
Feixiang Long,
No information about this author
Lizhan Su,
No information about this author
Mingxin Zhang
No information about this author
et al.
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(19), P. 10366 - 10366
Published: Sept. 26, 2024
Porcine
reproductive
and
respiratory
syndrome
(PRRS),
caused
by
PRRS
virus
(PRRSV)
infection,
has
been
a
serious
threat
to
the
pork
industry
worldwide
continues
bring
significant
economic
loss.
Current
vaccination
strategies
offer
limited
protection
against
PRRSV
transmission,
highlighting
urgent
need
for
novel
antiviral
approaches.
In
present
study,
we
reported
first
time
that
betulonic
acid
(BA),
widely
available
pentacyclic
triterpenoids
throughout
plant
kingdom,
exhibited
potent
inhibition
on
infections
in
both
Marc-145
cells
primary
porcine
alveolar
macrophages
(PAMs),
with
IC
Language: Английский