Antiviral activity of rosemary extract against Zika virus in human dermal fibroblasts

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 31, 2025

Abstract Flaviviruses have increasingly emerged and re-emerged in recent decades, infecting millions of people annually. Zika virus is particularly concerning due to its associated pathological complications, including microcephaly newborns Guillain-Barré syndrome adults, posing a significant threat public health. Despite efforts made by the scientific community, no licensed drugs against flaviviruses been developed. Medicinal plants show promise as novel source antiviral agents, they possess diverse array biologically active secondary metabolites, making them potential candidates for therapeutic use. We sought investigate rosemary extract (RE) ZIKV human dermal fibroblasts (HFF-1 cells), one earliest targets infection. was treated with various concentrations RE or individual major polyphenols, rosmarinic acid (RA), carnosic (CA), carnosol (CO), remaining infectivity each sample measured plaque reduction assay. To evaluate impact on different stages replication cycle, HFF-1 cells were before, during, after infection, before exerted potent activity both Vero directly acting particles Importantly, significantly inhibited later cycle interfering post-entry mechanisms within host cell. Moreover, RE-derived polyphenols CA CO, but not RA, shown reduce infectivity. Overall, impairs infection vitro interacting prior adsorption processes cycle. This study provides rigorous evidence indicating development an agent, warranting further investigation into underlying inhibitory effects other medically important flaviviruses.

Language: Английский

---

Diterpenes of Salvia (2016–2024): phytochemistry and pharmacology

Phytochemistry Reviews, Journal Year: 2025, Volume and Issue: unknown

Published: April 11, 2025

Language: Английский

---

Polyphenols Investigation and Antioxidant and Anticholinesterase Activities of Rosmarinus officinalis L. Species from Southwest Romania Flora

Molecules, Journal Year: 2024, Volume and Issue: 29(18), P. 4438 - 4438

Published: Sept. 18, 2024

Rosemary is one of the most important medicinal plants for natural therapy due to its multiple pharmacological properties, such as antioxidant, anti-inflammatory, neuroprotective, antiproliferative, antitumor, hepato- and nephroprotective, hypolipidemic, hypocholesterolemic, antihypertensive, anti-ischemic, hypoglycemic, radioprotective, antimicrobial, antiviral, antiallergic, wound healing properties. Our study reports first time, over a 12-month period, identification quantification polyphenols investigation antioxidant acetylcholinesterase (AChE) inhibitory activities Rosmarinus officinalis L. species harvested at flowering from flora southwestern Romania (Oltenia Region). Identification polyphenolic acids was made by ultra-high-performance liquid chromatography/mass spectrometry (UHPLC/MS). Total phenolic content determined using spectrophotometric method. In situ anticholinesterase were evaluated 2,2-diphenyl-1-picrylhydrazyl (DPPH) AChE assay, respectively, on high-performance thin-layer chromatography (HPTLC) plates. DPPH radical scavenging activity also assessed spectrophotometrically. The results revealed significant correlations between specific compounds measured biological activities, understanding role seasonal variations providing insights into optimal harvesting times benefits rosemary. research brings new information phytochemical profile R. source with

Language: Английский

---

Betulonic Acid Inhibits Type-2 Porcine Reproductive and Respiratory Syndrome Virus Replication by Downregulating Cellular ATP Production

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(19), P. 10366 - 10366

Published: Sept. 26, 2024

Porcine reproductive and respiratory syndrome (PRRS), caused by PRRS virus (PRRSV) infection, has been a serious threat to the pork industry worldwide continues bring significant economic loss. Current vaccination strategies offer limited protection against PRRSV transmission, highlighting urgent need for novel antiviral approaches. In present study, we reported first time that betulonic acid (BA), widely available pentacyclic triterpenoids throughout plant kingdom, exhibited potent inhibition on infections in both Marc-145 cells primary porcine alveolar macrophages (PAMs), with IC

Language: Английский

---