Chronic Gastrointestinal Disorders and miRNA-Associated Disease: An Up-to-Date

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(1), P. 413 - 413

Published: Jan. 6, 2025

Chronic gastrointestinal disorders such as inflammatory bowel diseases (IBDs) and irritable syndrome (IBS) impose significant health burdens globally. IBDs, encompassing Crohn’s disease ulcerative colitis, are multifactorial characterized by chronic inflammation of the tract. On other hand, IBS is one principal tract functional abdominal pain altered habits. Although precise etiopathogenesis these remains unclear, mounting evidence suggests that non-coding RNA molecules play crucial roles in regulating gene expression associated with inflammation, apoptosis, oxidative stress, tissue permeability, thus influencing progression. miRNAs have emerged possible reliable biomarkers, they can be analyzed biological fluids patients at a low cost. This review explores IBDs IBS, focusing on their involvement control hallmarks. By an extensive literature employing bioinformatics tools, we identified frequently studied concerning diseases. Ultimately, specific could proposed diagnostic biomarkers for IBS. Their ability to secreted into biofluids makes them promising candidates non-invasive tools. Therefore, understanding molecular mechanisms through ways which regulate immune responses provide new insights pathogenesis open avenues miRNA-based therapeutic interventions.

Language: Английский

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Macrophages and Gut Barrier Function: Guardians of Gastrointestinal Health in Post-Inflammatory and Post-Infection Responses

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(17), P. 9422 - 9422

Published: Aug. 30, 2024

The gut barrier is essential for protection against pathogens and maintaining homeostasis. Macrophages are key players in the immune system, indispensable intestinal health, contribute to defense repair mechanisms. Understanding multifaceted roles of macrophages can provide critical insights into restoring gastrointestinal (GI) health. This review explores role function their contribution post-inflammatory post-infectious responses gut. significantly integrity through epithelial repair, modulation, interactions with microbiota. They demonstrate active plasticity by switching phenotypes resolve inflammation, facilitate tissue regulate microbial populations following an infection or inflammation. In addition, tissue-resident (M2) infiltration (M1) convert each other problems such as IBS IBD via major signaling pathways mediated NF-κB, JAK/STAT, PI3K/AKT, MAPK, Toll-like receptors, specific microRNAs miR-155, miR-29, miR-146a, miR-199, which may be good targets new therapeutic approaches. Future research should focus on elucidating detailed molecular mechanisms developing personalized approaches fully harness potential maintain restore permeability

Language: Английский

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Immune-Mediated Bidirectional Causality Between Inflammatory Bowel Disease and Chronic Periodontitis: Evidence from Mendelian Randomization and Integrative Bioinformatics Analysis

Biomedicines, Journal Year: 2025, Volume and Issue: 13(2), P. 476 - 476

Published: Feb. 15, 2025

Background/Objectives: A bidirectional association between inflammatory bowel disease (IBD) and periodontitis has been observed, yet their causal relationship remains unclear. This study aimed to investigate the potential links these two conditions through comprehensive genetic molecular analyses. Methods: We conducted a Mendelian randomization (MR) analysis integrated with bioinformatics approaches. The relationships were primarily evaluated using inverse variance weighting (IVW), complemented by multiple sensitivity analyses assess robustness of findings. Additionally, we performed differential gene expression RNA sequencing data identify co-expressed genes shared mediators IBD periodontitis, followed pathway enrichment analysis. Results: Bidirectional MR revealed significant associations (p-value < 0.05). Sensitivity demonstrated consistency findings, no evidence heterogeneity or horizontal pleiotropy > Integrated identified key immune regulators, particularly interleukin 1 beta (IL1B) C-X-C motif chemokine receptor 4 (CXCR4), signaling pathways, including tumor necrosis factor (TNF-α) 17 (IL17), as mechanisms underlying conditions. Conclusions: Our findings provide supporting periodontitis. Transcriptomic pathological crucial regulatory factors common both diseases. These insights enhance our understanding interplay potentially informing new therapeutic strategies for

Language: Английский

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Single-Step Detection of microRNA via the Programmable DNAzyme Probe-Mediated Multiple Signal Recycling

ACS Omega, Journal Year: 2025, Volume and Issue: unknown

Published: March 25, 2025

The significant role of microRNAs (miRNAs) in the regulation pathological ocular neovascularization makes them both biomarkers and therapeutic targets vascular eye diseases. However, their acute sensitive detection continue to be a challenge. An integrative DNA probe (detection probe) was developed enable single-step miRNAs with high sensitivity specificity. This combines functions specific target recognition, DNAzyme unit-based signal reaction, polymerase/endonuclease-assisted cycle. In particular, single-stranded sequence that engages unit s2 can identify miRNA liberate facilitate reaction help metal ions. addition, recycling process cycle were initiated by chain extension displacement process, resulting low limit 4.56 fM. method demonstrated level septicity for capable distinguishing interfering one-base mismatch. Our research has shown nanomachine is promising biosensor single step. It anticipated this technology will used early diagnosis therapy

Language: Английский

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Right-Sided Dysplasia in Inflammatory Bowel Disease Is Not Associated with Conventional Risk Factors for Neoplasia

Gastroenterology Insights, Journal Year: 2025, Volume and Issue: 16(2), P. 14 - 14

Published: April 7, 2025

In the general population, right I-sided dysplasia presents a higher risk for colorectal cancer (CRC) and metachronous compared to left (L)-sided dysplasia. Given that patients with inflammatory bowel disease (IBD) are at than we sought assess factors as well differences in outcomes between R-sided, L-sided, both R- L-sided A retrospective chart review was performed on NYU Langone Health who had evidence of colonoscopy 2011 2021. Demographics pertinent medical history were compiled. Cohorts based location (R-sided, or L-sided) IBD-related analyzed. total 71 colonic The mean age 54 years old (SD ± 17). majority male (72%), white (69%), non-Hispanic (94%). 76% ulcerative colitis (UC) 24% Crohn's (CD). Of all dysplastic lesions, 57 (80%) unifocal remainder multifocal disease. 39 (55%) R-sided dysplasia, 24 (34%) 8 (11%) Patients UC more likely have (92% vs. 8% CD; p = 0.04). Pseudopolyps associated (38% 10% 4% dysplasia; 0.03). however, there no progression those Larger studies needed related laterality further validate these findings among IBD.

Language: Английский

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Expression Profile of Serum CircFUNDC1 and CircUHRF1 Can Differentiate Between Colorectal Cancer and Inflammatory Bowel Diseases (Ulcerative Colitis and Crohn's Disease)

Journal of Clinical Laboratory Analysis, Journal Year: 2025, Volume and Issue: unknown

Published: May 16, 2025

ABSTRACT Background Colorectal cancer ( CRC ) is a worldwide burden. Circular RNAs are promising biomarkers for diagnosing and prognosis of . Objective To investigate the possible association sera levels CircFUNDC1 CircUHRF1 expression with predisposition clinicopathological findings in , ulcerative colitis UC ), Crohn's disease CD Egyptian patients. Methods The serum were evaluated 113 subjects divided into four groups; (31), (26), (25) compared to healthy controls (31) using quantitative polymerase chain reaction. Results median values log2 fold change FC patients 9.11, 6.58, 6.17, respectively. It was upregulated all case groups. had significantly higher than p < 0.001). However, there no significant differences among patient groups ). medians log 2 −2.00, 3.33, 3.12, level lower group patients, difference between controls. Serum overexpressed or By Roc curve analysis, both genes can differentiate from inflammatory bowel IBD 0.05. Conclusion biomarker while

Language: Английский

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Identification of Angiogenesis-Related Gene Signatures and Prediction of Potential Therapeutic Targets in Ulcerative Colitis Using Integrated Bioinformatics

Journal of Inflammation Research, Journal Year: 2024, Volume and Issue: Volume 17, P. 11699 - 11717

Published: Dec. 1, 2024

This study aims to clarify angiogenesis mechanisms in ulcerative colitis and identify potential therapeutic targets.

Language: Английский

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Fibrosis-Related microRNAs in Crohn’s Disease with Fibrostenosis and Inflammatory Stenosis

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(16), P. 8826 - 8826

Published: Aug. 13, 2024

Crohn’s disease (CD) is frequently complicated by strictures that can be either inflammatory or fibrostenotic. This distinction important for deciding the best treatment course, but it difficult to determine clinically, sometimes even advanced imaging techniques. We performed miRNA PCR panel screening on pooled samples of ileum with CD fibrostenosis stenosis. Eight miRNAs profibrotic (miR-93-5p, miR-376c-3p and miR-424-5p), fibroprotective (miR-133a-3p, miR-133b, miR-193a-5p, miR-335-5p miR-378a-3p) functions described in literature were selected validation 20 each stenosis, a separate sampling submucosa subserosa. The results showed significant differences between groups subserosal samples, upregulation downregulation compared Only miR-424-5p difference submucosa. There expression subserosa Our provide further evidence major stenosis are located subserosa, which inaccessible endoscopic sampling, highlighting need cross-sectional serological markers. identify several previously not connected fibrosis CD, could potentially serve as biomarkers fibrostenosis.

Language: Английский

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