Identification, Phylogeny, and Expression Profiling of Pineapple Heat Shock Proteins (HSP70) Under Various Abiotic Stresses DOI Open Access
Rui Xu, Fangjun Wei,

Yanzhao Chen

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(24), P. 13407 - 13407

Published: Dec. 14, 2024

Pineapple (Ananas comosus (L.) Merr.) is an economically significant and delicious tropical fruit. commercial production faces severe decline due to abiotic stresses, which affect the development quality of pineapple Heat shock protein 70 (HSP70) plays essential role in stress tolerance. However, HSP70 family identification expression analysis response stresses has not been studied. To explore functional AcHSP70, different treatments were applied cultivar “Bali” seedlings. A total 21 AcHSP70 members identified genome. The genes classified into four subfamilies (I–IV) using phylogenetic analysis. expressed under conditions. Quantitative real time polymerase chain reaction (qRT-PCR) revealed pattern cold, drought, salt, heat stress. level such as AcHSP70-2 increased heat, drought stress, while AcHSP70-3 decreased salt Furthermore, profile AcHSP70s tissues stages was analyzed transcriptome exhibited unique patterns tissue at developmental stages. study therefore provides a list with substantial roles valuable information for understanding characteristics during tolerance pineapple.

Language: Английский

WRKY Transcription Factors (TFs) as Key Regulators of Plant Resilience to Environmental Stresses: Current Perspective DOI Creative Commons
Hui Li, Muneer Ahmed Khoso, Xu He

et al.

Agronomy, Journal Year: 2024, Volume and Issue: 14(10), P. 2421 - 2421

Published: Oct. 19, 2024

Plants encounter various stresses in their natural environments and can effectively respond to only one stress at a time. Through complex gene network, transcription factors (TFs) such as WRKY TFs regulate diverse array of responses. The clarification the structural characteristics proteins, along with recent advancements molecular dynamics simulations, has shed light on formation, stability, interactions DNA–protein complexes. This provided novel viewpoint regarding control TFs. investigation superfamilies, encompassing historical development, diversity, evolutionary patterns, become feasible due transcriptome approach’s capacity provide extensive comprehensive transcripts. significance lies pivotal role within several signaling cascades regulatory networks that influence plant defense present review summarizes functional aspects high-volume sequence data from different species studied date. Moreover, comparative analysis approach was utilized determine functions identified response both abiotic biotic stresses, revealed through numerous studies species. results this will be understanding events context climate change, incorporating new scientific evidence propose an innovative viewpoint.

Language: Английский

Citations

5
BPC1 and BPC2 positively regulates the waterlogging stress tolerance in Arabidopsis thaliana DOI
Jiaying Chen, Zhihui Liu, Jingwei Yan

et al.

Biochemical and Biophysical Research Communications, Journal Year: 2025, Volume and Issue: 747, P. 151296 - 151296

Published: Jan. 7, 2025

Language: Английский

Citations

0
Comprehensive transcriptome and proteome analysis revealed the molecular mechanisms of melatonin priming and waterlogging response in peach DOI Creative Commons
Xianbin Gu,

Linghong Lu,

Jing Gao

et al.

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 7, 2025

Waterlogging substantially hampers the growth and development of plants. The escalating trajectory global climate change is heightening both frequency intensity waterlogging events. Peach trees are particularly vulnerable to waterlogging, with resultant hypoxia in rhizosphere profoundly influencing their productivity. This study explored responses peach seedlings regulatory effects melatonin priming. After a 24-h treatment, significant increase relative electrical conductivity an accumulation reactive oxygen species were observed, ion permeability was markedly alleviated by Transcriptomic proteomic analyses conducted on root samples elucidate molecular mechanisms involved response Transcriptome analysis implicated genes related 'DNA-binding transcription factor activity', such as AP2/ERF, HSF WRKY factors, waterlogging. glycolysis/gluconeogenesis pathway also significantly enriched, indicating its critical role metabolic A correlation between differentially expressed proteins highlighted regulation numerous at transcriptional translational levels. Furthermore, core DEGs/DEPs, including heat shock stress-related proteins, identified. Notably, ERF VII member ERF071 (Prupe.8G264900), ADH (Prupe.8G018100), PCO (Prupe.7G011000) emerged potential targets for genetic manipulation enhance tolerance peach. research provides breeding waterlogging-tolerant varieties strategies mitigate stress

Language: Английский

Citations

0
Transcriptome analysis and genome-wide identification of WRKY gene family in Leonurus japonicus under drought stress DOI Creative Commons
Tongtong Guo, Qing Wang,

Yuxiao Yi

et al.

BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)

Published: May 9, 2025

Leonurus japonicus (L. japonicus) is a herbaceous flowering plant, widely distributed in Asia. Drought one of the primary environmental stress factors affecting L. growth. Previous studies have demonstrated that WRKY transcription (TFs) play crucial role plant responses to drought stress. So far, there has been no research on function genes japonicus. The physiological experiment results showed significantly increased malondialdehyde (MDA), proline, and hydrogen peroxide (H₂O₂) content Transcriptome analysis revealed significant changes expression levels gene family. Based bioinformatics analysis, 67 (LjWRKYs) were identified genome japonicus, with amino acid lengths ranging from 85 574. LjWRKYs can be divided into three subfamilies. Among them, LjWRKY (1/4/23/44) up-regulated under stress, whereas (21/25/65) down-regulated. Additionally, Kyoto Encyclopedia Genes Genomes (KEGG) indicated after differentially expressed (DEGs) enriched hormone signal transduction pathway, MAPK signaling pathway biosynthesis secondary metabolites pathway. In 19 DEGs, 9 which contained W-box regions, suggesting they may potential regulatory targets TFs These findings suggested family participate response This study provides scientific basis for further development functional validation Not applicable.

Language: Английский

Citations

0
Identification, Phylogeny, and Expression Profiling of Pineapple Heat Shock Proteins (HSP70) Under Various Abiotic Stresses DOI Open Access
Rui Xu, Fangjun Wei,

Yanzhao Chen

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(24), P. 13407 - 13407

Published: Dec. 14, 2024

Pineapple (Ananas comosus (L.) Merr.) is an economically significant and delicious tropical fruit. commercial production faces severe decline due to abiotic stresses, which affect the development quality of pineapple Heat shock protein 70 (HSP70) plays essential role in stress tolerance. However, HSP70 family identification expression analysis response stresses has not been studied. To explore functional AcHSP70, different treatments were applied cultivar “Bali” seedlings. A total 21 AcHSP70 members identified genome. The genes classified into four subfamilies (I–IV) using phylogenetic analysis. expressed under conditions. Quantitative real time polymerase chain reaction (qRT-PCR) revealed pattern cold, drought, salt, heat stress. level such as AcHSP70-2 increased heat, drought stress, while AcHSP70-3 decreased salt Furthermore, profile AcHSP70s tissues stages was analyzed transcriptome exhibited unique patterns tissue at developmental stages. study therefore provides a list with substantial roles valuable information for understanding characteristics during tolerance pineapple.

Language: Английский

Citations

0