Advanced Glycation End-Product-Modified Heat Shock Protein 90 May Be Associated with Urinary Stones

Diseases, Journal Year: 2025, Volume and Issue: 13(1), P. 7 - 7

Published: Jan. 2, 2025

Urinary stones (urolithiasis) have been categorized as kidney (renal calculus), ureteric (ureteral calculus and ureterolith), bladder (bladder urethral (urethral calculus); however, the mechanisms underlying their promotion related injuries in glomerular tubular cells remain unclear. Although lifestyle-related diseases (LSRDs) such hyperglycemia, type 2 diabetic mellitus, non-alcoholic fatty liver disease/non-alcoholic steatohepatitis, cardiovascular disease are risk factors for urolithiasis, Recently, heat shock protein 90 (HSP90) on membrane of HK-2 human proximal epithelium has associated with adhesion urinary cytotoxicity. Further, HSP90 pancreatic breast can be modified by various advanced glycation end-products (AGEs), thus affecting function. Hypothesis 1: We hypothesized that HSP90s on/in types AGEs, they may affect functions it a key to reveal LSRDs urolithiasis. 2: considered possibility Japanese traditional medicines urolithiasis inhibit AGE generation. Of Choreito Urocalun (the extract Quercus salicina Blume/Quercus stenophylla Makino) used clinic, is Kampo medicine, while characteristic medicine. As contains quercetin, hesperidin, p-hydroxy cinnamic acid, which generation, we generation AGE-modified cells.

Language: Английский

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The Advanced Glycation End-Products (AGE)–Receptor for AGE System (RAGE): An Inflammatory Pathway Linking Obesity and Cardiovascular Diseases

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(8), P. 3707 - 3707

Published: April 14, 2025

The AGE (advanced glycation end-products)–RAGE (receptor for AGE) system is a pro-inflammatory pathway that contributes to the pathogenesis of obesity and obesity-related cardiovascular disorders (CVD). Circulating soluble form RAGE (sRAGE) has been suggested as potential biomarker CVD related obesity. In this study, we aim (1) summarize current knowledge about role in onset progression CVD, (2) discuss AGE–RAGE promoting linking (3) highlight available strategies reducing activation associated beneficial effects.

Language: Английский

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WormCNN-Assisted Establishment and Analysis of Glycation Stress Models in C. elegans: Insights into Disease and Healthy Aging

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(17), P. 9675 - 9675

Published: Sept. 6, 2024

Glycation Stress (GS), induced by advanced glycation end-products (AGEs), significantly impacts aging processes. This study introduces a new model of GS Caenorhabditis elegans feeding them Escherichia coli OP50 cultured in glucose-enriched medium, which better simulates human dietary compared to previous single protein–glucose cross-linking methods. Utilizing WormCNN, deep learning model, we assessed the health status and calculated Healthy Aging Index (HAI) worms with or without GS. Our results demonstrated accelerated group, evidenced increased autofluorescence altered gene expression key regulators, daf-2 daf-16. Additionally, observed elevated pharyngeal pumping rates AGEs-fed worms, suggesting an addictive response similar patterns. highlights profound effects on worm underscores critical role computer vision accurately assessing aiding establishment disease models. The findings provide insights into glycation-induced offer comprehensive approach studying

Language: Английский

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Slot Blot- and Electrospray Ionization–Mass Spectrometry/Matrix-Assisted Laser Desorption/Ionization–Mass Spectrometry-Based Novel Analysis Methods for the Identification and Quantification of Advanced Glycation End-Products in the Urine

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(17), P. 9632 - 9632

Published: Sept. 5, 2024

Proteins, saccharides, and low molecular organic compounds in the blood, urine, saliva could potentially serve as biomarkers for diseases related to diet, lifestyle, use of illegal drugs. Lifestyle-related (LSRDs) such diabetes mellitus (DM), non-alcoholic steatohepatitis, cardiovascular disease, hypertension, kidney osteoporosis develop into life-threatening conditions. Therefore, there is an urgent need their early diagnosis. Advanced glycation end-products (AGEs) are associated with LSRDs may induce/promote LSRDs. The presence AGEs body fluids represent a biomarker Urine samples be used detecting AGEs, urine collection convenient non-invasive. However, detection identification AGE-modified proteins challenging, concentrations might extremely low. To address this issue, we propose new analytical approach. This strategy employs method previously introduced by us, which combines slot blotting, our unique lysis buffer named Takata’s buffer, polyvinylidene difluoride membrane, conjunction electrospray ionization-mass spectrometry (ESI)/matrix-assisted laser desorption/ionization-mass (MALDI-MS). novel detect proteins, peptides, free-type samples.

Language: Английский

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Antioxidant and anti-glycation activities of Mandevilla velutina extract and effect on parasitemia levels in Trypanosoma cruzi experimental infection: in vivo, in vitro and in silico approaches

Journal of Ethnopharmacology, Journal Year: 2024, Volume and Issue: unknown, P. 118994 - 118994

Published: Oct. 1, 2024

Language: Английский

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