Beyond Chemotherapy: Exploring 5-FU Resistance and Stemness in Colorectal Cancer
European Journal of Pharmacology,
Journal Year:
2025,
Volume and Issue:
unknown, P. 177294 - 177294
Published: Jan. 1, 2025
Language: Английский
Computational Elucidation of Hub Genes and Pathways Correlated with the Development of 5-Fluorouracil Resistance in HCT 116 Colorectal Carcinoma Cell Line
Biochemical Genetics,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 30, 2025
Language: Английский
Pathological Investigation of the Effect of Bovine Colostrum Against 5-FU-Induced Liver, Kidney, and Heart Toxicity in Rats
Life,
Journal Year:
2025,
Volume and Issue:
15(4), P. 564 - 564
Published: March 31, 2025
This
study
aimed
to
investigate
the
possible
histopathological
and
immunohistochemical
effects
of
bovine
colostrum
(BC)
against
toxic
5-fluorouracil
(5-FU)
on
liver,
kidney,
heart
Wistar
Albino
rats.
Animals
were
divided
into
three
groups:
control,
5-FU,
5-FU+BC.
The
control
group
received
2
mL/kg
i.p.
saline,
5-FU
100
mg/kg
5-FU+BC
saline
first
day
study.
groups
1000
BC
orally
each
Liver,
tissues
examined
histopathologically
for
lesions
expression
TNF-α,
HSP-27,
CASP-3,
8-OHdG.
No
pathologic
observed
in
group,
whereas
severe
group.
In
less
than
examination,
biomarker
was
not
it
At
end
study,
that
5-FU-induced
pathological
findings
decreased
with
use
colostrum.
difference
between
significant
(p
<
0.01
p
0.05,
respectively).
Although
addition
did
show
any
statistical
significance
scores
tissues,
improved
these
tissues.
Nevertheless,
analyses
showed
visible
improvements
more
studies
are
needed,
is
hoped
will
improve
prognosis
by
both
reducing
side
a
good
chemotherapeutic
agent,
its
antineoplastic
properties.
Language: Английский
Targeting colon cancer and normal cells with cold plasma-activated water: Exploring cytotoxic effects and cellular responses
Physics of Plasmas,
Journal Year:
2024,
Volume and Issue:
31(8)
Published: Aug. 1, 2024
Plasma-activated
water
(PAW),
generated
by
cold
plasma,
is
emerging
as
a
potential
treatment
for
colon
cancer.
This
study
focused
on
its
anticancer
effects
against
HCT-116
cancer
cells,
emphasizing
the
role
of
pH
and
conductivity
variations
due
to
plasma–fluid
reactions.
These
changes
suggest
chemical
transformation
in
PAW,
leading
increased
acidity
ion
presence.
The
cytotoxic
impact
PAW
cells
was
analyzed
using
methods
like
enzyme-linked
immunosorbent
assay
microscopic
evaluation.
exhibited
cytotoxicity
but
also
affected
normal
posing
challenge
selectivity.
An
18
h
exposure
duration
identified
balance
between
cell
eradication
preservation.
Observed
morphological
indicated
apoptotic
characteristics
PAW-treated
hinting
at
mechanisms
death.
PAW-induced
reactive
oxygen
species
release
mirrored
cellular
stress,
with
early
markers,
DNA
fragmentation,
heat
shock
proteins
(HSPs)
signifying
complex
responses.
findings
that
can
trigger
apoptosis
stress
pathways
cells.
However,
further
studies
are
necessary
therapy.
Language: Английский
In-Silico and In-Vitro Investigation of Key Long Non-coding RNAs Involved in 5-Fluorouracil Resistance in Colorectal Cancer Cells: Analyses Highlighting NEAT1 and MALAT1 as Contributors
Cureus,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Aug. 7, 2024
Background
Acquired
resistance
to
5-fluorouracil
(5-FU)
frequently
results
in
chemotherapy
failure
and
disease
recurrence
advanced
colorectal
cancer
(CRC)
patients.
Research
has
demonstrated
that
dysregulation
of
long
non-coding
RNAs
(lncRNAs)
mediates
the
development
cancerous
cells.
The
present
study
aims
identify
key
lncRNAs
associated
with
5-FU
CRC
using
bioinformatic
experimental
validation
approaches.
Methods
Gene
Expression
Omnibus
(GEO)
dataset
GSE119481,
which
contains
miRNA
expression
profiles
parental
HCT116
cell
line
(HCT116/P)
its
in-vitro
established
5-FU-resistant
sub-cell
(HCT116/FUR),
was
downloaded.
Firstly,
differentially
expressed
microRNAs
(DEmiRNAs)
between
cells
were
identified.
LncRNAs
mRNAs
then
predicted
online
databases.
Ontology
(GO)
Kyoto
Encyclopedia
Genes
Genomes
(KEGG)
enrichment
analyses
performed
uncover
relevant
biological
mechanisms
pathways.
Networks
integrating
lncRNAs,
miRNAs,
interactions
constructed,
topological
used
resistance.
An
model
HCT116/FUR
developed
by
exposing
HCT116/P
increasing
concentrations
5-FU.
Finally,
real-time
quantitative
PCR
(RT-qPCR)
on
total
RNA
extracted
from
validate
in-silico
predictions
lncRNAs.
Results
A
32
DEmiRNAs
Enrichment
analysis
these
mainly
enriched
several
hallmark
pathways
regulate
growth,
cycle,
survival,
inflammation,
immune
response,
apoptosis.
predictive
identified
237
unique
123
interacting
DEmiRNAs.
pathway
indicated
most
genes
cellular
response
starvation,
protein
polyubiquitination,
chromatin
remodeling,
negative
regulation
gene
expression.
Topological
lncRNA-miRNA-mRNA
network
highlighted
nuclear
abundant
transcript
1
(NEAT1),
metastasis-associated
lung
adenocarcinoma
(MALAT1),
Opa
5
antisense
(OIP5-AS1)
as
central
Experimental
RT-qPCR
confirmed
levels
NEAT1
MALAT1
significantly
increased
compared
However,
no
significant
difference
observed
OIP5-AS1
level
two
Conclusion
Our
findings
specifically
highlight
contributors
CRC.
These
are
promising
biomarkers
for
diagnosing
predicting
outcomes
Language: Английский
Possible Combinatorial Utilization of Phytochemicals and Extracellular Vesicles for Wound Healing and Regeneration
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(19), P. 10353 - 10353
Published: Sept. 26, 2024
Organ
and
tissue
damage
can
result
from
injury
disease.
How
to
facilitate
regeneration
has
been
a
topic
for
centuries,
still,
we
are
trying
find
agents
use
treatments.
Two
groups
of
biological
substances
known
wound
healing.
Phytochemicals
with
bioactive
properties
form
one
group.
Many
phytochemicals
have
anti-inflammatory
effects
enhance
Recent
studies
described
their
at
the
gene
protein
expression
levels,
highlighting
receptors
signaling
pathways
involved.
The
extremely
large
number
multiple
types
they
activate
suggest
broad
range
applicability
clinical
use.
hydrophobic
nature
many
difficulty
chemical
stabilization
problem.
developments
in
biotechnology
nanotechnology
methods
enabling
researchers
overcome
these
problems.
other
group
is
extracellular
vesicles
(EVs),
which
now
important
functions,
including
improvement
proteins
nanoparticles
contained
mammalian
EVs
as
well
specificity
targets
microRNAs
included
becoming
clear.
Plant-derived
found
contain
phytochemicals.
overlap
wound-healing
capabilities
both
differences
possibility
combinatorial
two
groups,
may
effects.
Language: Английский