Phytochemical, In Silico, In Vitro, and In Vivo Research on Piptadeniastrum africanum (Fabaceae) Unveiling Anti‐Stereotypic, Anxiolytic, and Analgesic Effects in a Sodium Valproate‐Induced Autistic Disorders Model DOI Creative Commons

A Julie,

Omer Bébé Ngouateu, Mengue Ngadena Yolande Sandrine

et al.

Brain and Behavior, Journal Year: 2025, Volume and Issue: 15(3)

Published: March 1, 2025

ABSTRACT Objective Individuals with autistic spectrum disorders (ASD) primarily exhibit deficits in communication and social interaction, along repetitive behaviors restricted interests. This disorder is often associated anxiety, nociceptive disorders, pain. While medical treatment generally focuses on treating the symptoms rather than addressing underlying causes, traditional medicine sometimes used as an alternative. Piptadeniastrum africanum Cameroonian medicinal folks to treat cognitive disorders. However, its effects mechanisms of action regarding inhibition ASD‐like remain unclear. The primary goal present study was evaluate anxiolytic analgesic water extract P. triad induced rats by sodium valproate. Material Methods investigated secondary metabolites using UHPLC‐MS. DPPH, ABTS, FRAP tests were performed assess extract's ability neutralize free radicals. Molecular docking utilized binding various receptors. For experimental study, 33 pregnant female divided into two groups after pregnancy confirmed. One group given distilled orally at 10 mL/kg, while other received valproate 800 mg/kg gestation days 11, 12, 13. When male offspring reached 3 weeks old, they evaluated for pain sensitivity, those displaying any selected further study. remaining split six five treated either a vehicle, bumetanide, or 190 760 mg/kg. Behavioral assessments focusing sociability, sensitivity conducted 28 37 weaning. In end, biochemical markers related GABA metabolism, serotonin levels, oxidative status analyzed cerebellum, prefrontal cortex, hippocampus, amygdala alongside histopathological analyses brain. Results UHPLC‐MS allows us identify several compounds. They bind H3R (7F61) HDAC2 through conventional hydrogen bonding. Findings showed that prenatal administration deficit interaction ( p < 0.001), anxiety hypersensitivity increased concentration disturbed neuronal loss 0.001) well disorganization cerebellum young compared neurotypical animals. doses used, like corrected these protected against loss. These results suggest has anti‐nociceptive effects. It been found positive can be achieved restoring GABAergic serotonergic neurotransmission, coupled antioxidant neuromodulatory activity. Conclusion current findings support induces valproate‐induced model.

Language: Английский

Phytochemical, In Silico, In Vitro, and In Vivo Research on Piptadeniastrum africanum (Fabaceae) Unveiling Anti‐Stereotypic, Anxiolytic, and Analgesic Effects in a Sodium Valproate‐Induced Autistic Disorders Model DOI Creative Commons

A Julie,

Omer Bébé Ngouateu, Mengue Ngadena Yolande Sandrine

et al.

Brain and Behavior, Journal Year: 2025, Volume and Issue: 15(3)

Published: March 1, 2025

ABSTRACT Objective Individuals with autistic spectrum disorders (ASD) primarily exhibit deficits in communication and social interaction, along repetitive behaviors restricted interests. This disorder is often associated anxiety, nociceptive disorders, pain. While medical treatment generally focuses on treating the symptoms rather than addressing underlying causes, traditional medicine sometimes used as an alternative. Piptadeniastrum africanum Cameroonian medicinal folks to treat cognitive disorders. However, its effects mechanisms of action regarding inhibition ASD‐like remain unclear. The primary goal present study was evaluate anxiolytic analgesic water extract P. triad induced rats by sodium valproate. Material Methods investigated secondary metabolites using UHPLC‐MS. DPPH, ABTS, FRAP tests were performed assess extract's ability neutralize free radicals. Molecular docking utilized binding various receptors. For experimental study, 33 pregnant female divided into two groups after pregnancy confirmed. One group given distilled orally at 10 mL/kg, while other received valproate 800 mg/kg gestation days 11, 12, 13. When male offspring reached 3 weeks old, they evaluated for pain sensitivity, those displaying any selected further study. remaining split six five treated either a vehicle, bumetanide, or 190 760 mg/kg. Behavioral assessments focusing sociability, sensitivity conducted 28 37 weaning. In end, biochemical markers related GABA metabolism, serotonin levels, oxidative status analyzed cerebellum, prefrontal cortex, hippocampus, amygdala alongside histopathological analyses brain. Results UHPLC‐MS allows us identify several compounds. They bind H3R (7F61) HDAC2 through conventional hydrogen bonding. Findings showed that prenatal administration deficit interaction ( p < 0.001), anxiety hypersensitivity increased concentration disturbed neuronal loss 0.001) well disorganization cerebellum young compared neurotypical animals. doses used, like corrected these protected against loss. These results suggest has anti‐nociceptive effects. It been found positive can be achieved restoring GABAergic serotonergic neurotransmission, coupled antioxidant neuromodulatory activity. Conclusion current findings support induces valproate‐induced model.

Language: Английский

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