Characterization of Circulating Vesicles of Complicated and Uncomplicated Systemic Sclerosis Patients and Their Role in Vascular Dysfunction DOI Open Access
Elena Grossini, Mattia Bellan, Sakthipriyan Venkatesan

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(6), P. 2380 - 2380

Published: March 7, 2025

Extracellular vesicles (EVs) could be involved in the onset of systemic sclerosis (SSc) through modulation vascular function. Anyway, available data are contradictory, and further investigation would necessary to clarify this aspect. Here, we characterized circulating EVs isolated from SSc patients evaluated their effects on human endothelial cells (HUVECs) smooth muscle cells. In 13 complicated 27 uncomplicated five healthy controls (HCs), analyzed size, concentration, surface marker expression. addition, were used stimulate HUVECs, cell viability, mitochondrial membrane potential, nitric oxide (NO) reactive oxygen species (MitoROS) release. cells, calcium movement examined. The results showed that expressed markers T-lymphocyte/platelet/endothelial origin larger more concentrated than those HCs. reduced viability potential increased NO MitoROS release HUVECs intracellular conclusion, found a specific pattern for patients, which have pathogenic role direct actions

Language: Английский

Maternal Serum Catestatin Levels in Gestational Diabetes Mellitus: A Potential Biomarker for Risk Assessment and Diagnosis DOI Open Access
Nazan Vanlı Tonyalı, Gülşan Karabay, Burak Arslan

et al.

Journal of Clinical Medicine, Journal Year: 2025, Volume and Issue: 14(2), P. 435 - 435

Published: Jan. 11, 2025

Background/Objectives: Gestational diabetes mellitus (GDM) presents significant risks for both maternal and neonatal health, affecting fetal growth increasing the likelihood of future (DM) development in affected women. The dysregulation metabolic biomarkers, including catestatin, has been implicated GDM pathophysiology. However, clinical significance catestatin remains poorly understood, particularly context different therapeutic approaches. Methods: This observational, prospective, cross-sectional study was conducted to evaluate serum levels gestational patients healthy controls. Data were collected at a single time point during second trimester pregnancy (24 28 weeks). Participants categorized based on their glucose tolerance management strategies (diet regulation or insulin therapy). Results: Receiver Operating Characteristic (ROC) analysis demonstrated diagnostic GDM, suggesting cut-off value >9.61 ng/mL discriminating between women with without GDM. further research is needed elucidate mechanistic role its utility guiding interventions. Conclusions: Our highlights potential as biomarker risk stratification monitoring, complementing existing tools. Integrating biomarkers like into approaches may optimize health outcomes limitations our study, design sample size, underscore need multicenter studies validate findings comprehensively.

Language: Английский

Citations

2

Characterization of Circulating Vesicles of Complicated and Uncomplicated Systemic Sclerosis Patients and Their Role in Vascular Dysfunction DOI Open Access
Elena Grossini, Mattia Bellan, Sakthipriyan Venkatesan

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(6), P. 2380 - 2380

Published: March 7, 2025

Extracellular vesicles (EVs) could be involved in the onset of systemic sclerosis (SSc) through modulation vascular function. Anyway, available data are contradictory, and further investigation would necessary to clarify this aspect. Here, we characterized circulating EVs isolated from SSc patients evaluated their effects on human endothelial cells (HUVECs) smooth muscle cells. In 13 complicated 27 uncomplicated five healthy controls (HCs), analyzed size, concentration, surface marker expression. addition, were used stimulate HUVECs, cell viability, mitochondrial membrane potential, nitric oxide (NO) reactive oxygen species (MitoROS) release. cells, calcium movement examined. The results showed that expressed markers T-lymphocyte/platelet/endothelial origin larger more concentrated than those HCs. reduced viability potential increased NO MitoROS release HUVECs intracellular conclusion, found a specific pattern for patients, which have pathogenic role direct actions

Language: Английский

Citations

0