The clots removed from ischaemic stroke patients by mechanical thrombectomy are amyloid in nature DOI Creative Commons
Justine M. Grixti, Arun Chandran,

Johann Pretorius

et al.

medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 4, 2024

Abstract Ischemic stroke due to large vessel occlusion results from the blockage of a major cerebral artery by clot; however, origins and molecular composition these clots remain poorly understood. Mechanical thrombectomy has become standard treatment remove obstructive clots, providing unique opportunity analyze their properties. We previously demonstrated that blood can clot into an amyloid-like form, generating fibrinaloid microclots (2–200 μm) are highly resistant fibrinolysis. In this study, archived eight ischemic patients with were examined, using samples stored in Walton Centre Clot Bank Liverpool, UK. All exhibited strong, heterogeneous amyloid staining, revealing pervasive component. These findings represent unreported characteristic highlighting potential for amyloid-targeted therapies overcome fibrinolytic resistance foundational new insight ischaemic pathophysiology treatment.

Language: Английский

Proteomic signatures of Post-Vaccination/Post-Infection Syndrome (PV/PIS): Insights into immune dysregulation and coagulopathy DOI

Maxine Waters,

Maré Vlok,

Elouise E. Kroon

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: May 5, 2025

Abstract During the global rollout of COVID-19 vaccines a subset individuals reported persistent symptoms following vaccination, with clinical presentations overlapping those Long COVID requiring individualised treatment decisions. Distinguishing between vaccine-related adverse events and post-infectious sequelae remains challenging, particularly given possibility asymptomatic or mild SARS-CoV-2 infection prior to after vaccination. To avoid this complexity, we define patient group as presenting Post-Vaccination/Post-Infection Syndrome (PV/PIS). In study, performed proteomic analysis plasma from 30 PV/PIS compared healthy controls. Using mass spectrometry, identified significant alterations in coagulation factors, acute phase proteins, immune response modulators group. Notably, elevated levels serum amyloid A1 A2, attractin, factors X XI were observed, alongside downregulation immune-regulatory proteins. These findings suggest that is characterized by dysregulation coagulopathy, signatures only partially previously proteomics on samples collected vaccination availability. Our results highlight complex interplay activation, endothelial dysfunction, pathologies PV/PIS, while also highlighting distinct differences these systems paving way for more targeted protein research conditions.

Language: Английский

Citations

0
The clots removed from ischaemic stroke patients by mechanical thrombectomy are amyloid in nature DOI Creative Commons
Justine M. Grixti, Arun Chandran,

Johann Pretorius

et al.

medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 4, 2024

Abstract Ischemic stroke due to large vessel occlusion results from the blockage of a major cerebral artery by clot; however, origins and molecular composition these clots remain poorly understood. Mechanical thrombectomy has become standard treatment remove obstructive clots, providing unique opportunity analyze their properties. We previously demonstrated that blood can clot into an amyloid-like form, generating fibrinaloid microclots (2–200 μm) are highly resistant fibrinolysis. In this study, archived eight ischemic patients with were examined, using samples stored in Walton Centre Clot Bank Liverpool, UK. All exhibited strong, heterogeneous amyloid staining, revealing pervasive component. These findings represent unreported characteristic highlighting potential for amyloid-targeted therapies overcome fibrinolytic resistance foundational new insight ischaemic pathophysiology treatment.

Language: Английский

Citations

1