Increased Prolylcarboxypeptidase Expression Can Serve as a Biomarker of Senescence in Culture DOI Creative Commons

Nicholas Boullard,

Jason J. Paris,

Zia Shariat‐Madar

et al.

Molecules, Journal Year: 2024, Volume and Issue: 29(10), P. 2219 - 2219

Published: May 9, 2024

Prolylcarboxypeptidase (PRCP, PCP, Lysosomal Pro-X-carboxypeptidase, Angiotensinase C) controls angiotensin- and kinin-induced cell signaling. Elevation of PRCP appears to be activated in chronic inflammatory diseases [cardiovascular disease (CVD), diabetes] proportion severity. Vascular endothelial senescence mitochondrial dysfunction have consistently been shown models CVD aging. Cellular senescence, a driver age-related dysfunction, can differentially alter the expression lysosomal enzymes due membrane permeability. There is lack data demonstrating effect on function PRCP. To explore changes PRCP, PRCP-dependent prekallikrein (PK) pathway was characterized early- late-passage human pulmonary artery cells (HPAECs). Detailed kinetic analysis treated with high molecular weight kininogen (HK), precursor bradykinin (BK), PK revealed mechanism by which senescent HPAECs activate generation kallikrein upon assembly HK–PK complex parallel an upregulation nitric oxide (NO) synthase (eNOS) NO formation. The production both eNOS increased early-passage decreased HPAECs. Low activity associated rapid telomerase reverse transcriptase mRNA levels. We also found that, increase passage number HPAECs, reduced altered respiration rate. These results indicated that aging dysregulates protein expression, further studies will shed light into complexity signaling

Language: Английский

[Chemico-Biological Interactions] In vitro and in vivo anti-inflammatory effects of 5-hydroxyconiferaldehyde via NF-κB, MAPK/AP-1, and Nrf2 modulation DOI
Soo‐Yeon Kim, Jae‐Min Kim, Kyung‐Sook Chung

et al.

Chemico-Biological Interactions, Journal Year: 2025, Volume and Issue: unknown, P. 111427 - 111427

Published: Feb. 1, 2025

Language: Английский

Citations

1
Relationship between Oxidative Stress and Left Ventricle Markers in Patients with Chronic Heart Failure DOI Creative Commons
Aušra Mongirdienė,

Agnė Liuizė,

Dovilė Karčiauskaitė

et al.

Cells, Journal Year: 2023, Volume and Issue: 12(5), P. 803 - 803

Published: March 4, 2023

Oxidative stress is proposed in the literature as an important player development of CHF and correlates with left ventricle (LV) dysfunction hypertrophy failing heart. In this study, we aimed to verify if serum oxidative markers differ chronic heart failure (CHF) patients' groups depending on LV geometry function. Patients were stratified into two according ventricular ejection fraction (LVEF) values: HFrEF (<40% (n = 27)) HFpEF (≥40% 33)). Additionally, patients four geometry: NG-normal 7), CR-concentric remodeling 14), cLVH-concentric 16), eLVF-eccentric 23). We measured protein (protein carbonyl (PC), nitrotyrosine (NT-Tyr), dityrosine), lipid (malondialdehyde (MDA), oxidizes (HDL) oxidation antioxidant (catalase activity, total plasma capacity (TAC) serum. Transthoracic echocardiogram analysis lipidogram also performed. found that (NT-Tyr, dityrosine, PC, MDA, oxHDL) antioxidative (TAC, catalase) marker levels did not between LVEF or geometry. NT-Tyr correlated PC (rs 0.482, p 0.000098), oxHDL 0.278, 0.0314). MDA 0.337, 0.008), LDL 0.295, 0.022) non-HDL 0.301, 0.019) cholesterol. negatively HDL cholesterol -0.285, 0.027). parameters correlate oxidative/antioxidative markers. Significant negative correlations end-diastolic volume end-systolic HDL-cholesterol -0.935, < 0.0001; rs -0.906, 0.0001, respectively). positive both thickness interventricular septum wall triacylglycerol 0.346, 0.007; 0.329, 0.010, respectively) found. conclusions, find a difference concentrations oxidant MDA) (TAC function was The could be related metabolism patients, no correlation oxidative/antioxidant

Language: Английский

Citations

10
Non-invasive vagus nerve stimulation in anti-inflammatory therapy: mechanistic insights and future perspectives DOI Creative Commons
Fujun Liu, Jing Wu,

Lijun Gong

et al.

Frontiers in Neuroscience, Journal Year: 2024, Volume and Issue: 18

Published: Nov. 13, 2024

Non-invasive vagus nerve stimulation (VNS) represents a transformative approach for managing broad spectrum of inflammatory and autoimmune conditions, including rheumatoid arthritis bowel disease. This comprehensive review delineates the mechanisms underlying VNS, emphasizing cholinergic anti-inflammatory pathway, explores interactions within neuro-immune vagus-gut axes based on both clinical outcomes pre-clinical models. Clinical applications have confirmed efficacy VNS in specific diseases, such as arthritis, chronic conditions like disease, showcasing variability parameters patient responses. Concurrently, studies provided insights into potential modulating cardiovascular broader responses, paving way its translational application settings. Innovations non-invasive technology precision neuromodulation are enhancing therapeutic potential, making it viable option patients who unresponsive to conventional treatments. Nonetheless, widespread adoption this promising therapy is impeded by regulatory challenges, compliance issues, need extensive long-term safety. Future research directions will focus refining technology, optimizing treatment parameters, exploring synergistic effects with other modalities, which could revolutionize management disorders.

Language: Английский

Citations

3
Modulation of MAPK/NF-κB Pathway and NLRP3 Inflammasome by Secondary Metabolites from Red Algae: A Mechanistic Study DOI Creative Commons
Asmaa Nabil-Adam, Mohamed L. Ashour, Mohamed A. Shreadah

et al.

ACS Omega, Journal Year: 2023, Volume and Issue: 8(41), P. 37971 - 37990

Published: Oct. 5, 2023

The pharmacological properties of seaweeds are diverse. No studies have been conducted on the protective effect Galaxaura oblongata (GOE) against lippopolysaccharide (LPS)-induced inflammation in brain. This study is divided into three phases, first which initial phase. In vitro includes antioxidant, radical scavenging, and anti-inflammatory activities, including cyclooxygenase-1 (COX1), COX2, NO, acetylcholine inhibition, sphingosine kinase 1, tumor necrosis factor α (TNF-α), interleukin-6, as well antioxidant radical-scavenging 2,2-diphenyl-1-picrylhydrazyl 2,2'-azinobis(3-ethylbenzothiazoline)-6-sulfonic acid. Using LPS-induced acute inflammation, second phase was vivo. Antioxidant assays were performed to investigate role GOE. addition phytochemical analysis, bioactive content GOE also investigated. results demonstrated potential an anti-inflammatory, neuroprotective agent. A using LPS induced lung injury neuroinflammation model confirmed results. significantly reduced inflammatory, oxidative, neurodegenerative biomarkers based histopathological immuno-histochemistry Based computational drug design, four target proteins approved: nuclear κB, mitogen-activated protein kinases, TNF-α, NLRP3. polyphenolic compounds ligands good alignment affinity proteins. Finally, current offers a new approach developing leads considering GOE's curative roles.

Language: Английский

Citations

6
Management of Obesity-Related Inflammatory and Cardiovascular Diseases by Medicinal Plants: From Traditional Uses to Therapeutic Targets DOI Creative Commons
Bashar Saad

Biomedicines, Journal Year: 2023, Volume and Issue: 11(8), P. 2204 - 2204

Published: Aug. 5, 2023

Inflammation is a crucial factor in the development and progression of cardiovascular diseases (CVD). Cardiac remodeling presence persistent inflammation leads to myocardial fibrosis extracellular matrix changes, which reduce cardiac function, induce arrhythmias, finally, cause heart failure. The majority current CVD treatment plans concentrate on reducing risk factors such as hyperlipidemia, type 2 diabetes, hypertension. One strategy could be reduction. Numerous vitro, animal, clinical studies indicate that obesity associated with low-grade inflammation. Recent have demonstrated potential medicinal plants phytochemicals cure prevent In comparison conventional therapies, synergistic effects several boost their bioavailability impact numerous cellular molecular targets. Focusing appetite, pancreatic lipase activity, thermogenesis, lipid metabolism, lipolysis adipogenesis, apoptosis adipocytes, adipocyte life cycle by represent an important goal new anti-obesity drugs. We conducted extensive review literature electronic databases, including Google Scholar, PubMed, Science Direct, MedlinePlus, for collecting data therapeutic plants/phytochemicals curing its related diseases, mechanisms, cytokines, signal transduction cascades, trials.

Language: Английский

Citations

5
From gains to gaps? How Selective Androgen Receptor Modulator (SARM) YK11 impact hippocampal function: In silico, in vivo, and ex vivo perspectives DOI
Mustafa Munir Mustafa Dahleh, Vandreza Cardoso Bortolotto, Silvana Peterini Boeira

et al.

Chemico-Biological Interactions, Journal Year: 2024, Volume and Issue: 394, P. 110971 - 110971

Published: March 21, 2024

Language: Английский

Citations

1
Increased Prolylcarboxypeptidase Expression Can Serve as Biomarker of Senescence in Culture DOI Open Access

Nicholas Boullard,

Jason J. Paris,

Zia Shariat‐Madar

et al.

Published: March 28, 2024

Prolylcarboxypeptidase (PRCP, PCP, Lysosomal Pro-X-carboxypeptidase, Angiotensinase C) controls angiotensin - and kinin- induced cell signaling. Elevation of PRCP appear to be activated in chronic inflammatory diseases (cardiovascular disease (CVD), diabetes) proportion severity. Vascular endothelial senescence mitochondrial dysfunction have consistently been shown models CVD aging. The cellular senescence, a driver age-related dysfunction, can differentially alter the expression lysosomal enzymes due membrane permeability. There is lack data demostrating effect on function PRCP. To explore changes PRCP, PRCP-dependent prekallikrein (PK) pathway was charcterized early – late-passage human pulmonary artery cells (HPAECs). Detailed kinetic analysis treated with high molecular weight kininogen (HK), precursor bradykinin (BK), PK revealed mechansim by which senescent HPAECs activate generation kallikrein upon assembly HK-PK complex parallel an upregulation nitric oxide (NO) synthase (eNOS) NO formation. production both eNOS increased early-passage while decreased HPAECs. Low activity late passage associated rapid telomerase reverse transcriptase mRNA levels. We also found that, increase number HPAECs, reduced altered respiration rate. These results indicated that aging dysregulates protein expression, further studies will shed light into complexity signling

Language: Английский

Citations

1
Anti-atherogenic mechanism of ethanol extract of Christia vespertilionis (L.f.) Bakh. F. Leaves in vitro DOI

Jiah Ning Tan,

Khairana Husain, Zakiah Jubri

et al.

International Immunopharmacology, Journal Year: 2024, Volume and Issue: 134, P. 112148 - 112148

Published: May 7, 2024

Language: Английский

Citations

1
Increased Prolylcarboxypeptidase Expression Can Serve as a Biomarker of Senescence in Culture DOI Creative Commons

Nicholas Boullard,

Jason J. Paris,

Zia Shariat‐Madar

et al.

Molecules, Journal Year: 2024, Volume and Issue: 29(10), P. 2219 - 2219

Published: May 9, 2024

Prolylcarboxypeptidase (PRCP, PCP, Lysosomal Pro-X-carboxypeptidase, Angiotensinase C) controls angiotensin- and kinin-induced cell signaling. Elevation of PRCP appears to be activated in chronic inflammatory diseases [cardiovascular disease (CVD), diabetes] proportion severity. Vascular endothelial senescence mitochondrial dysfunction have consistently been shown models CVD aging. Cellular senescence, a driver age-related dysfunction, can differentially alter the expression lysosomal enzymes due membrane permeability. There is lack data demonstrating effect on function PRCP. To explore changes PRCP, PRCP-dependent prekallikrein (PK) pathway was characterized early- late-passage human pulmonary artery cells (HPAECs). Detailed kinetic analysis treated with high molecular weight kininogen (HK), precursor bradykinin (BK), PK revealed mechanism by which senescent HPAECs activate generation kallikrein upon assembly HK–PK complex parallel an upregulation nitric oxide (NO) synthase (eNOS) NO formation. The production both eNOS increased early-passage decreased HPAECs. Low activity associated rapid telomerase reverse transcriptase mRNA levels. We also found that, increase passage number HPAECs, reduced altered respiration rate. These results indicated that aging dysregulates protein expression, further studies will shed light into complexity signaling

Language: Английский

Citations

1