Interspecies Isobaric Labeling-Based Quantitative Proteomics Reveals Protein Changes in the Ovary of Aedes aegypti Coinfected With ZIKV and Wolbachia DOI Creative Commons
Luís Felipe Costa Ramos, Michele Martins, Jimmy Rodriguez Murillo

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2022, Volume and Issue: 12

Published: July 7, 2022

Zika is a vector-borne disease caused by an arbovirus (ZIKV) and overwhelmingly transmitted Ae. aegypti . This linked to adverse fetal outcomes, mostly microcephaly in newborns, other clinical aspects such as acute febrile illness neurologic complications, for example, Guillain-Barré syndrome. One of the most promising strategies mitigate transmission involves releasing mosquitoes carrying maternally inherited endosymbiont bacteria Wolbachia pipientis The presence associated with reduced susceptibility arboviruses fitness cost mosquito life-history traits fecundity fertility. However, mechanisms which influences metabolic pathways leading differences egg production remains poorly known. To investigate impact coinfections on reproductive tract mosquito, we applied isobaric labeling-based quantitative proteomic strategy influence w Mel ZIKV infection ovaries. best our knowledge, this complete proteome ovaries reported so far, total 3913 proteins identified, were also able quantify 1044 complex sample tissue ovary. Furthermore, from 480 modulated study, discuss altered during infections, coinfection /ZIKV, compared no infection, focusing immune modified mainly related priming enhancement modulation Juvenile Hormone pathway both microorganism’s infection.

Language: Английский

Infertility and fecundity loss of Wolbachia-infected Aedes aegypti hatched from quiescent eggs is expected to alter invasion dynamics DOI Creative Commons
Meng‐Jia Lau, Perran A. Ross, Ary A. Hoffmann

et al.

PLoS neglected tropical diseases, Journal Year: 2021, Volume and Issue: 15(2), P. e0009179 - e0009179

Published: Feb. 16, 2021

The endosymbiotic bacterium Wolbachia shows viral blocking in its mosquito host, leading to use arboviral disease control. Releases with strains w Mel and AlbB infecting Aedes aegypti have taken place several countries. Mosquito egg survival is a key factor influencing population persistence this trait also important when eggs are stored prior releases. We therefore tested the viability of mosquitoes derived from AlbB-infected as well uninfected after long-term storage under diurnal temperature cycles 11–19°C 22–30°C. Eggs at had higher hatch proportions than those Adult density declined they emerged for longer, which was associated incomplete cytoplasmic incompatibility (CI) Mel-infected males were crossed females. Females both temperatures continued show perfect maternal transmission , but reduced fecundity females relative mosquitoes. Furthermore, we found very strong negative impact infection on fertility 22–30°C, almost 80% hatching 11 weeks being infertile. Our findings provide guidance storing -infected A . ensure high fitness adult release. Importantly, highlight likely quiescence dynamics populations field, potential suppress through cumulative costs across warm dry periods, expected effects dengue transmission.

Language: Английский

Citations

62
Successful introgression of wMel Wolbachia into Aedes aegypti populations in Fiji, Vanuatu and Kiribati DOI Creative Commons
Cameron P. Simmons,

Wesley Donald,

Lekon Tagavi

et al.

PLoS neglected tropical diseases, Journal Year: 2024, Volume and Issue: 18(3), P. e0012022 - e0012022

Published: March 14, 2024

Pacific Island countries have experienced periodic dengue, chikungunya and Zika outbreaks for decades. The prevention control of these mosquito-borne diseases rely heavily on Aedes aegypti mosquitoes, which in most settings are the primary vector. Introgression intracellular bacterium Wolbachia pipientis ( w Mel strain) into Ae . populations reduces their vector competence consequently lowers dengue incidence human population. Here we describe successful area-wide deployments Mel-infected Suva, Lautoka, Nadi (Fiji), Port Vila (Vanuatu) South Tarawa (Kiribati). With community support, weekly releases mosquitoes between 2 to 5 months resulted introgression nearly all locations. Long term monitoring confirmed a high, self-sustaining prevalence infecting almost deployment areas. Measurement public health outcomes were disrupted by Covid19 pandemic but expected emerge coming years.

Language: Английский

Citations

6
A standalone incompatible insect technique enables mosquito suppression in the urban subtropics DOI Creative Commons
Qin Zeng,

Lingzhi She,

Hao Yuan

et al.

Communications Biology, Journal Year: 2022, Volume and Issue: 5(1)

Published: Dec. 27, 2022

Abstract The strong suppression of Aedes albopictus on two Guangzhou islands in China has been successfully achieved by releasing males with an artificial triple- Wolbachia infection. However, it requires the use radiation to sterilize residual females prevent population replacement. To develop a highly effective tool for dengue control, we tested standalone incompatible insect technique (IIT) control A. urban area Changsha, inland city where recently emerged. Male mosquitoes were produced mass rearing facility and transported over 670 km under low temperature release site. After once-per-week high numbers (phase I) subsequent twice-per-week II), average hatched eggs female adults collected weekly per trap reduced 97% 85%, respectively. caused 94% decrease mosquito biting at site compared Remarkably, this was using only 28% number released previous trial. Despite lack irradiation females, no triple-infected detected field post based monitoring adult larval populations years, indicating that replacement prevented. Our results support feasibility implementing IIT areas.

Language: Английский

Citations

27
Symbiotic Wolbachia in mosquitoes and its role in reducing the transmission of mosquito-borne diseases: updates and prospects DOI Creative Commons
Awoke Minwuyelet, Giulio Petronio Petronio, Delenasaw Yewhalaw

et al.

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: Oct. 13, 2023

Mosquito-borne diseases such as malaria, dengue fever, West Nile virus, chikungunya, Zika and filariasis have the greatest health economic impact. These mosquito-borne are a major cause of morbidity mortality in tropical sub-tropical areas. Due to lack effective vector containment strategies, prevalence severity these increasing endemic regions. Nowadays, mosquito infection by endosymbiotic Wolbachia represents promising new bio-control strategy. Wild-infected mosquitoes had been developing cytoplasmic incompatibility (CI), phenotypic alterations, nutrition competition with pathogens. reduce adult lifespan, interfere reproduction, inhibit other pathogen growth vector, increase insecticide susceptibility vector. Wild, uninfected can also establish stable infections through trans-infection advantage adaptability defense, thereby selectively infecting spreading entire population. This review aimed evaluate role symbiont ( Aedes, Anopheles, Culex ) reducing diseases. Global databases PubMed, Web Sciences, Scopus, pro-Quest were accessed search for potentially relevant articles. We used keywords: , Anopheles Aedes alone or combination during literature search. Data extracted from 56 articles’ texts, figures, tables included article.

Language: Английский

Citations

15
Wolbachia strain wAlbB shows favourable characteristics for dengue control use in Aedes aegypti from Burkina Faso DOI Creative Commons
Maria Vittoria Mancini, Shivan M. Murdochy, Etienne Bilgo

et al.

Environmental Microbiology, Journal Year: 2024, Volume and Issue: 26(3)

Published: March 1, 2024

Abstract Dengue represents an increasing public health burden worldwide. In Africa, underreporting and misdiagnosis often mask its true epidemiology, dengue is likely to be both more widespread than reported data suggest in incidence distribution. Wolbachia ‐based control underway Asia the Americas but has not date been deployed Africa. Due genetic heterogeneity of African Aedes aegypti populations complexity host‐symbiont interactions, characterization key parameters ‐carrying mosquitoes paramount for determining potential system as a tool The w AlbB strain was stably introduced into Ae. population by introgression, showed high intracellular density whole bodies different mosquito tissues; also maintained following larval rearing at temperatures. No effect on adult lifespan induced presence detected. Moreover, ability this strongly inhibit DENV‐2 dissemination transmission host demonstrated background. Our findings harnessing .

Language: Английский

Citations

5
Designing effective Wolbachia release programs for mosquito and arbovirus control DOI
Perran A. Ross

Acta Tropica, Journal Year: 2021, Volume and Issue: 222, P. 106045 - 106045

Published: July 15, 2021

Language: Английский

Citations

29
MicroRNAs reshape the immunity of insects in response to bacterial infection DOI Creative Commons
Muhammad Nadeem Abbas, Saima Kausar,

Bibi Asma

et al.

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14

Published: April 21, 2023

The interaction between bacteria and insects can significantly impact a wide range of different areas because are widely distributed around the globe. bacterial-insect interactions have potential to directly affect human health since vectors for disease transmission, their also economic consequences. In addition, they been linked high mortality rates in economically important insects, resulting substantial losses. MicroRNAs (miRNAs) types non-coding RNAs involved regulating gene expression post-transcriptionally. length miRNAs ranges from 19 22 nucleotides. MiRNAs, addition ability exhibit dynamic patterns, diverse targets. This enables them govern various physiological activities like innate immune responses. Increasing evidence suggests that crucial biological role bacterial infection by influencing responses other mechanisms resistance. review focuses on some most recent exciting discoveries made years, including correlation dysregulation miRNA context progression infection. Furthermore, it describes how profoundly host targeting Toll, IMD, JNK signaling pathways. It emphasizes function insects. Finally, discusses current knowledge gaps about insect immunity, require more research future.

Language: Английский

Citations

12
Wolbachia-Based Emerging Strategies for Control of Vector-Transmitted Disease DOI Creative Commons
Diego Montenegro, Gerardo Cortés-Cortés,

María Guadalupe Balbuena-Alonso

et al.

Acta Tropica, Journal Year: 2024, Volume and Issue: unknown, P. 107410 - 107410

Published: Sept. 1, 2024

Language: Английский

Citations

4
Targeted knockdown of in vitro candidates does not alter Wolbachia density in vivo DOI Creative Commons
Kimberley R. Dainty, Johanna M. Duyvestyn, Heather A. Flores

et al.

Journal of Invertebrate Pathology, Journal Year: 2025, Volume and Issue: unknown, P. 108346 - 108346

Published: April 1, 2025

Language: Английский

Citations

0
Unveiling Wolbachia transcriptomic signature in the arboviral vector Aedes aegypti DOI Creative Commons
Sebastián Mejías, Natalia E. Jiménez, Carlos Conca

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: April 28, 2025

Introduction The mosquito Aedes aegypti is the main vector of arboviral diseases such as dengue and imposes a global health burden. A promising control strategy to infect A. populations with Wolbachia , genus intracellular bacteria capable blocking infections. Enhancing preserving efficacy this method will depend on solid mechanistic knowledge aegypti-Wolbachia symbiosis. By identifying differences between -infected uninfected previous transcriptomic studies proposed wide range symbiotic interactions, but systematic identification consistent effects across datasets still missing. Methods To identify genes functions consistently affected by we performed differential expression functional enrichment analysis published datasets, followed meta-analysis obtained p-values using maxP method. Six were retrieved from Gene Expression Omnibus, Sequence Read Archive ArrayExpress (last searched in July 2024, considering lack replication exclusion criteria). After discarding one dataset w AlbB-infected cell line due poor mapping genome, data comprised adult female heads, muscles, carcasses, midguts bodies, strains wMel wMelPop. Results Discussion Meta-analysis revealed 10 21 down- upregulated host genes, some which have escaped focus research, including downregulated exonuclease AAEL009650 has pro-dengue virus homolog Drosophila . At function level, found upregulation electron transport chain (ETC), carbohydrate serine-type peptidase activity inhibition, downregulation DNA replication. ETC suggests an alternative mechanism for Wolbachia’s induction antiviral oxidative stress, previously attributed dual- NADPH-oxidases here showed or no regulation. Through work identifies molecular targets future aimed at elucidating most fundamental mechanisms aegypti–Wolbachia

Language: Английский

Citations

0