Scientific Data, Journal Year: 2025, Volume and Issue: 12(1)
Published: Feb. 12, 2025
Language: Английский
FEMS Microbiology Reviews, Journal Year: 2023, Volume and Issue: 47(2)
Published: March 1, 2023
Galleria mellonella (greater wax moth) larvae are used widely as surrogate infectious disease models, due to ease of use and the presence an innate immune system functionally similar that vertebrates. Here, we review G. mellonella-human intracellular bacteria pathogen infection models from genera Burkholderia, Coxiella, Francisella, Listeria, Mycobacterium. For all genera, has increased understanding host-bacterial interactive biology, particularly through studies comparing virulence closely related species and/or wild-type versus mutant pairs. In many cases, in mirrors found mammalian although it is unclear whether pathogenic mechanisms same. The speeded up vivo efficacy toxicity testing novel antimicrobials treat infections caused by bacteria: area will expand since FDA no longer requires animal for licensure. Further mellonella-intracellular be driven advances genetics, imaging, metabolomics, proteomics, transcriptomic methodologies, alongside development accessibility reagents quantify markers, which underpinned a fully annotated genome.
Language: Английский
Citations
31
Frontiers in Genetics, Journal Year: 2023, Volume and Issue: 14
Published: June 9, 2023
The relationship between insect pathogenic fungi and their hosts is a classic example of co-evolutionary arms race pathogen target host: parasites evolve towards mechanisms that increase advantage over the host, host increasingly strengthens its defenses. present review summarizes literature data describing direct indirect role lipids as an important defense mechanism during fungal infection. Insect comprise anatomical physiological barriers, cellular humoral response mechanisms. entomopathogenic have unique ability to digest cuticle by producing hydrolytic enzymes with chitin-, lipo- proteolytic activity; besides oral tract, pays way for entry within host. key factor in resistance infection presence certain types (free fatty acids, waxes or hydrocarbons) which can promote inhibit attachment cuticle, might also antifungal activity. Lipids are considered source energy, triglycerides stored fat body, structure analogous liver adipose tissue vertebrates. In addition, body plays innate immunity range bactericidal proteins polypeptides, one lysozyme. Energy derived from lipid metabolism used hemocytes migrate site infection, phagocytosis, nodulation encapsulation. One polyunsaturated acid, arachidonic synthesis eicosanoids, play several crucial roles physiology immunology. Apolipoprotein III compound activity, modulate signal molecule.
Language: Английский
Citations
26
Global Change Biology, Journal Year: 2023, Volume and Issue: 29(22), P. 6248 - 6260
Published: Sept. 1, 2023
Bee populations are exposed to multiple stressors, including land-use change, biological invasions, climate and pesticide exposure, that may interact synergistically. We analyze the combined effects of warming sublethal insecticide exposure in solitary bee Osmia cornuta. Previous studies show warm wintering temperatures cause body weight loss, lipid consumption, fat depletion. Because plays a key role xenobiotic detoxification, we expected bees scenarios would be more sensitive pesticides. O. cornuta females three treatments: current scenario (2007-2012 temperatures), near-future (2021-2050 projected distant-future (2051-2080). Upon emergence spring, were orally doses an (Closer, a.i. sulfoxaflor; 0, 4.55 11.64 ng a.i./bee). measured on phototactic response, syrup longevity. Wintering treatment by itself did not affect winter mortality, but loss increased with increasing temperatures. Similarly, hardly influenced response or consumption. However, wintered at warmest had shorter longevity, strong fecundity predictor Osmia. Insecticide especially high dose, impaired ability respond light, resulted reduced consumption The combination dose 70% longevity decrease. Smaller bees, resulting from smaller pollen-nectar provisions, suggesting nutritional stress further compromise Our results synergistic interaction between two major drivers declines, indicate will become pesticides under global scenario. findings have important implications for regulation underscore need consider stressors understand declines.
Language: Английский
Citations
23
Insects, Journal Year: 2025, Volume and Issue: 16(1), P. 37 - 37
Published: Jan. 2, 2025
Thermal condition affects the development and growth of ectotherms. The stenothermic honeybee brood, particularly prepupae, are sensitive to low rearing temperature. fat body plays important roles in energy reserve metabolism during brood development. To date, metabolic changes prepupae responding cold stress have not been completely understood. In this study, ultra-performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS)-based non-target metabolome was analyzed between cold-treated (CT, 20 °C, 36 h) control (CK, 35 °C) prepupal honeybees. metabolomic data showed that levels 1860 254 metabolites were significantly increased decreased, respectively, cold-stressed prepupae. These altered metabolites, glutamine, glutamic acid, pyroglutamic oxidized glutathione, enriched into glutamine glutathione pathways. Furthermore, expression metabolism-related genes, glutaminase (GLS), glutamate dehydrogenase (GDH), gamma-glutamyl transferase (GGT-1 GGT-7), decreased cold-exposed compared groups. Meanwhile, (GSSG), but reduced (GSH) content, group controls. Collectively, our revealed larva-to-pupa transition when exposed stress. Our provided new insights sensitivity cold, characterized by perturbation oxidative
Language: Английский
Citations
1
Insects, Journal Year: 2025, Volume and Issue: 16(1), P. 97 - 97
Published: Jan. 18, 2025
Honey bees are known for their wide global distribution, ease of handling, and economic ecological value. However, they often exposed to a variety stress factors. Therefore, it is essential beekeepers maintain healthy bee colonies. In this context, balanced diet recommended support the growth strong honey The purpose review therefore provide an overview nutritional requirements Apis mellifera importance maintenance An adequate includes consumption sufficient amounts proteins, carbohydrates, lipids, amino acids, vitamins, minerals, water, sterols, based on multi-floral pollen desirable. when colonies located near agroecosystems with lower resource diversity, both brood rearing colony longevity may decrease, making them more susceptible parasites diseases. On other hand, efforts have been made improve health help supplements consisting components. Nevertheless, studies shown that even these supplements, lack nutrients can still be issue Furthermore, future research should focus identifying better replicate natural diversity assessing long-term effects resilience, especially in low-flowering areas. This discusses interaction between nutrient health.
Language: Английский
Citations
1
Pest Management Science, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 24, 2025
Abstract BACKGROUND The fall armyworm (FAW), Spodoptera frugiperda is a notorious invasive pest that poses serious threat to global agricultural production and food security. In China, Cotesia ruficrus has emerged as promising indigenous biological control agent for controlling FAW populations. this study, we conducted series of comprehensive experiments explore the effects C. parasitization on development nutrient metabolism larvae. RESULTS Parasitization by significantly inhibited larvae, reduced their intake more than 60%. Furthermore, concentrations carbohydrate triglyceride increased from third fifth day seventh after parasitization, respectively. However, both levels subsequently decreased during later stages. Transcriptome analysis showed there were 2096 differentially expressed genes (DEGs) between parasitized non‐parasitized These DEGs found be primarily enriched in pathways related immune response, detoxification, lipid synthesis metabolism, protein metabolism. CONCLUSION Taken together, these results strongly indicate ability modulate ultimately benefiting successful parasitoid offspring. findings contribute our understanding intricate interactions host insects natural enemies provide valuable insights into sustainable effective strategies control. © 2025 Society Chemical Industry.
Language: Английский
Citations
1
PeerJ, Journal Year: 2021, Volume and Issue: 9, P. e12563 - e12563
Published: Dec. 22, 2021
Insects are the most widespread group of organisms and more than one million species have been described. These animals significant ecological functions, for example they pollinators many types plants. However, also direct influence on human life in different manners. They high medical veterinary significance, stemming from their role as vectors disease infection wounds necrotic tissue; plant pests, parasitoids predators whose activities can agriculture. In addition, use treatments, such maggot therapy gangrene wounds, has grown considerably. uses forensic science to determine minimum post-mortem interval provide valuable information about movement body, cause death, drug use, or poisoning. It proposed that may be used model replace mammal systems research. The present review describes free fatty acids (FFAs) key physiological processes insects. By focusing insects medical, we limited our description those important point view insect control; study examines effects reproduction resistance adverse abiotic (low temperature) biotic (pathogens) factors.
Language: Английский
Citations
46
Molecules, Journal Year: 2024, Volume and Issue: 29(11), P. 2605 - 2605
Published: June 1, 2024
When studying honey bee nutrition, it is important to pay attention not only the quantity but also quality of pollen for floral visitors. The recommended way determine value both protein concentration and amino acid composition in insect’s hemolymph. In addition, includes lipids, sterols biogenic elements such as carbon, nitrogen, etc. Very high observed aloe pollen, averaging 51%. Plants with a content, at level 27% Europe, are rapeseed phacelia. turn, plant that poor (at 11%) buckwheat. aforementioned plants sown over very large areas. Vast acreages Central Eastern Europe occupied by pollen- nectar-providing invasive plants, goldenrod. Therefore, bees forced use one food source—a mono diet—which results their malnutrition. absence natural beekeepers other foods bees; including soy protein, powdered milk, egg yolks, fish meal, However, colony strongest when fed opposed artificial diets. More research needed on relationship between measured apian hemolymph, strength, yield good overwintering.
Language: Английский
Citations
7
Environmental Pollution, Journal Year: 2024, Volume and Issue: 348, P. 123783 - 123783
Published: March 13, 2024
Language: Английский
Citations
6
Current Opinion in Insect Science, Journal Year: 2022, Volume and Issue: 52, P. 100924 - 100924
Published: April 26, 2022
Most insects exhibit high reproductive capacity, which demands large amounts of energy, including macronutrients and micronutrients. Interestingly, many proteins involved in oogenesis depend on metals ions, particular iron (Fe), zinc (Zn), copper (Cu). Mechanisms by metal ions influence reproduction have been described Drosophila melanogaster, but remain poorly understood hematophagous where blood meals include significant ingestion ions. Moreover, there is evidence that some immunity could dual function both processes. This review highlights the importance non-hematophagous insects. In addition, we discuss how optimize physiological processes using crosstalk between physiology immunity, a double-edge sword allocating their functions to protect insect ensure reproduction.
Language: Английский
Citations
26