Dual RNA Sequencing of Beauveria bassiana-Infected Spodoptera frugiperda Reveals a Fungal Protease with Entomopathogenic and Antiphytopathogenic Activities

Journal of Agricultural and Food Chemistry, Journal Year: 2023, Volume and Issue: 71(34), P. 12757 - 12774

Published: Aug. 21, 2023

Insect pests and phytopathogens significantly impact crop yield quality. The fall armyworm (FAW) Spodoptera frugiperda the phytopathogen Fusarium graminearum cause substantial economic losses in crops like barley wheat. However, entomopathogen Beauveria bassiana shows limited efficacy against FAW, its antiphytopathogenic activities F. remain unclear. Here, dual RNA sequencing was performed to identify differentially expressed genes B. bassiana-infected FAW larvae. We found that BbAorsin gene upregulated at 36 48 h post-infection. encodes a serine-carboxyl protease is mainly blastospores hyphae. Overexpression of ARSEF2860 enhanced virulence Galleria mellonella larvae inhibited growth. recombinant protein induced apoptosis necrosis hemocytes spore germination. These findings shed light on transcriptomic mechanisms governing insect-pathogen interactions, which could aid developing dual-functional entomopathogens anti-phytopathogens.

Language: Английский

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UV‐induced mutagenesis of Beauveria bassiana (Hypocreales: Clavicipitaceae) yields two hypervirulent isolates with different transcriptomic profiles

Pest Management Science, Journal Year: 2023, Volume and Issue: 79(8), P. 2762 - 2779

Published: March 14, 2023

The fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae) can infest over 300 plant species and cause huge economic losses. Beauveria bassiana (Hypocreales: Clavicipitaceae) is one of the most widely used entomopathogenic fungi (EPF). Unfortunately, efficacy B. against S. quite low. Hypervirulent EPF isolates be obtained by ultraviolet (UV)-irradiation. Here we report on UV-induced mutagenesis transcriptomic analysis bassiana.The wild-type (WT) (ARSEF2860) was exposed to UV light induce mutagenesis. Two mutants (named 6M 8M) showed higher growth rates, conidial yields, germination rates compared WT strain. levels tolerance osmotic, oxidative, stresses. protease, chitinase, cellulose, chitinase activities than WT. Both were compatible with insecticides matrine, spinetoram, chlorantraniliprole, but incompatible emamectin benzoate. Insect bioassays that both more virulent greater wax moth Galleria mellonella. Transcriptomic profiles determined RNA-sequencing. differentially expressed genes (DEGs) identified. gene set enrichment (GSEA), protein-protein interaction (PPI) network, hub revealed virulence-related genes.Our data demonstrate UV-irradiation a very efficient economical technique improve virulence stress resistance bassiana. Comparative provide insights into genes. These results new ideas for improving genetic engineering field EPF. © 2023 Society Chemical Industry.

Language: Английский

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Spodoptera frugiperda (Lepidoptera: Noctuidae) Life Table Comparisons and Gut Microbiome Analysis Reared on Corn Varieties

Insects, Journal Year: 2023, Volume and Issue: 14(4), P. 358 - 358

Published: April 4, 2023

The fall armyworm (Spodoptera frugiperda, FAW) is an invasive migratory pest that has recently spread to Korea, damaging several corn cultivars with significant economic value. Comparisons of the growth stages FAW were conducted based on preferred feed. Therefore, we selected six maize cultivars, including three categories: (i) commercial waxy (mibaek 2-ho, heukjeom dreamoak); (ii) popcorn (oryun popcorn, oryun 2-ho); and (iii) processing (miheukchal). A effect was observed during larvae period, pupal egg hatching ratio, weight, whereas total survival period adult did not show variation among tested cultivars. We identified variations in gut bacterial community dependent genotype phyla included Firmicutes, Proteobacteria, Actinobacteria, Bacteroidetes. Among these genera, most abundant genus Enterococcus, followed by Ureibacillus. Enterococcus mundtii top 40 species. intergenic PCR-based amplification gene sequence colony isolates also matched GenBank owing prevalence E. mundtii. These results showed diversity abundance particular bacteria guts FAWs influenced major

Language: Английский

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Adverse effects of the fungal biopesticide Beauveria bassiana on a predatory social wasp

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 908, P. 168202 - 168202

Published: Oct. 31, 2023

Biopesticides are considered eco-friendly alternatives to synthetic agrochemicals. However, their impact on non-target organisms is still poorly understood. Social wasps, in particular, a largely neglected group when it comes risk assessment of plant protection products, despite the relevant ecological and economic services provided by these insects. In present study, we evaluated common biopesticide, entomopathogenic fungus Beauveria bassiana, paper wasp Polistes dominula. We adopted holistic approach ecotoxicology focusing not only detrimental effects isolated individuals, but also whole colony. Both adult wasps belonging different castes immature larvae were topically exposed field-realistic concentration fungal spores from commercial strain B. bassiana ATCC 74040 assess biopesticide survival, behavior physiology. Our results showed that causes number adverse P. dominula, include increased mortality, altered locomotion feeding rate, selective ejection nests, reduced oviposition rate ovary development foundresses, colony failure. findings provide new insights often-neglected sublethal pollutants can jeopardize individual beneficial insects, delicate social balance colonies valuable ecosystem services, highlighting natural origin plant-protection products does always guarantee environmental safety.

Language: Английский

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Lethal, Sub-Lethal and Trans-Generational Effects of Chlorantraniliprole on Biological Parameters, Demographic Traits, and Fitness Costs of Spodoptera frugiperda (Lepidoptera: Noctuidae)

Insects, Journal Year: 2022, Volume and Issue: 13(10), P. 881 - 881

Published: Sept. 28, 2022

Fall armyworm [Spodoptera frugiperda (J. E. Smith, 1797)] was first reported in the Americas, then spread to all continents of world. Chemical insecticides are frequently employed managing fall armyworms. These have various modes actions and target sites kill insects. Chlorantraniliprole is a selective insecticide with novel mode action used against Lepidopteran, Coleopteran, Isopteran, Dipteran pests. This study determined chlorantraniliprole’s lethal, sub-lethal, trans-generational effects on two consecutive generations (F0, F1, F2) armyworm. Bioassays revealed that chlorantraniliprole exhibited higher toxicity armyworms LC50 2.781 mg/L after 48 h exposure. Significant differences were noted biological parameters generations. Sub-lethal concentrations showed prolonged larval adult durations. The related fitness cost F0 F1 non-significant differences. In contrast, F2 generation lower fecundity at lethal (71 eggs/female) sub-lethal (94 doses compared control (127.5–129.3 eggs/female). Age-stage specific survival rate (Sxj), life expectancy (Exj) reproductive (Vxj) significantly differed among insecticide-treated groups control. A comparison treated untreated insects over indicated substantial demographic such as net reproduction (R0), intrinsic increase (r), mean time (T). Several shown be negatively impacted by chlorantraniliprole. We conclude may utilized manage lesser risks.

Language: Английский

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