Cryo-EM structures of ryanodine receptors and diamide insecticides reveal the mechanisms of selectivity and resistance

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Oct. 20, 2024

Language: Английский

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Genome-wide analysis of detoxification genes conferring diamide insecticide resistance in Spodoptera exigua identifies CYP9A40

Chemosphere, Journal Year: 2024, Volume and Issue: 367, P. 143623 - 143623

Published: Nov. 1, 2024

For over a decade, diamide insecticides have been effective against lepidopteran pests like beet armyworm, Spodoptera exigua (Hübner, 1808). However, the evolution of resistance poses challenge to their sustainable use. We identified an I4790 M mutation in S. ryanodine receptor (RyR) gene, but its correlation with varied across field-collected Korean populations exigua. RNA sequencing and differential gene expression analysis were performed investigate other mechanisms. Diamide-resistant susceptible strains F1 hybrids compared by mapping RNA-seq reads reference genome. CYP9A40 was as critical due high resistant strains. Synergist bioassays piperonyl butoxide supported role P450s metabolic A strong positive between over-expression levels (up 80-fold) LC

Language: Английский

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The ryanodine receptor mutation I4728M confers moderate‐level resistance to diamide insecticides in Spodoptera litura

Pest Management Science, Journal Year: 2023, Volume and Issue: 79(10), P. 3693 - 3699

Published: May 15, 2023

The common cutworm, Spodoptera litura (Fabricius), is one of the most widespread and destructive polyphagous pests in tropical subtropical Asia. S. has evolved resistance to different insecticides, including diamide insecticides. Here, we identified a ryanodine receptor (RyR) mutation (I4728M) associated with target site diamides field-collected population litura. contribution this was investigated through establishing near-isogenic resistant strain litura.The ND21 litura, collected from Ningde, Fujian province China 2021, exhibited 130.6-fold chlorantraniliprole compared susceptible NJ-S strain. RyR I4728M, corresponding Plutella xylostella I4790M, population. SlRyR I4728M introgressed into background (NJ-S) marker-assisted backcrossing. named ND21-R, which homozygous for mutant 4728M allele, shared about 94% genetic ND21-R showed moderate levels two anthranilic (19.1-fold chlorantraniliprole, 19.7-fold cyantraniliprole) phthalic flubendiamide (23.4-fold). Genetic analysis that autosomal, incompletely recessive tightly linked litura.Identification its will help develop allelic discrimination assays monitoring guide management practices © 2023 Society Chemical Industry.

Language: Английский

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Characterization of Six Diamide Insecticides on Ryanodine Receptor: Resistance and Species Selectivity

Journal of Agricultural and Food Chemistry, Journal Year: 2023, Volume and Issue: 71(29), P. 11001 - 11007

Published: July 18, 2023

Ryanodine receptor (RyR) has been used as an insecticide target to control many destructive agricultural pests. The effectiveness of these insecticides limited by the spread resistance mutations identified in pest RyRs, but detailed molecular impacts individual on activity different diamide compounds have not fully explored. We created five HEK293 cell lines stably expressing wild type rabbit RyR1, Spodoptera frugiperda RyR (Sf RyR), or Sf carrying mutations, including G4891E, G4891E/I4734M, and Y4867F, respectively. R-CEPIA1er, a genetically encoded fluorescent protein, was also introduced report Ca2+ concentration endoplasmic reticulum. systematically characterized activities six commercial against RyRs using time-lapse fluorescence assay. Among them, cyantraniliprole (CYAN) displayed highest all three resistant RyRs. good performance CYAN confirmed toxicity assay gene-edited Drosophila mutant which showed lowest LD50 value for double mutant. In addition, we compared their acitivty between mammalian insect found that flubendiamide best insect-selectivity. mechanism anti-resistance property selectivity proposed based structural models generated homology modeling docking. Our findings provide insights into guidance developing effective agonists can selectively

Language: Английский

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Exaptation of I4760M mutation in ryanodine receptor of Spodoptera exigua (Lepidoptera: Noctuidae): Lessons from museum and field samples

Pesticide Biochemistry and Physiology, Journal Year: 2023, Volume and Issue: 195, P. 105579 - 105579

Published: Aug. 12, 2023

Language: Английский

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Trends towards Lower Susceptibility of Spodoptera frugiperda (Lepidoptera: Noctuidae) to Teflubenzuron in Brazil: An Evidence for Field-Evolved Resistance

Insects, Journal Year: 2023, Volume and Issue: 14(2), P. 129 - 129

Published: Jan. 26, 2023

Susceptibility monitoring to insecticides is a key component implementing insecticide resistance management (IRM) programs. In this research, the susceptibility teflubenzuron in Spodoptera frugiperda (J.E Smith) was monitored more than 200 field-collected populations from major corn-growing regions of Brazil, 2004 2020. Initially, we defined diagnostic concentration 10 µg mL-1 using diet-overlay bioassay for susceptibility. A variation S. detected among different locations. We also significant reduction throughout time all evaluated, with larval survival at varying values <5% up 80% Thus, research provides evidence field-evolved and reinforces that IRM practices are urgently needed be implemented Brazil.

Language: Английский

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Molecular Characterization of Spodoptera frugiperda Heme Oxygenase and Its Involvement in Susceptibility to Chlorantraniliprole

Journal of Agricultural and Food Chemistry, Journal Year: 2023, Volume and Issue: 71(5), P. 2313 - 2321

Published: Jan. 27, 2023

The mammalian heme oxygenase (HO) plays an important role in cytoprotection against oxidative-stress-induced cell damage; however, functional characterization of insect HO is still limited. In this study, cDNA encoding a HO, named SfHO, was cloned from Spodoptera frugiperda. Analysis the transcription level and enzymatic activity showed that exposure LC30 concentration chlorantraniliprole to third instar larvae significantly upregulated both mRNA SfHO at 24 h after treatment. Further injection activator, hemin, into led upregulation as well decreased susceptibility S. frugiperda chlorantraniliprole. Consistently, overexpression increased Sf9 viability under Strikingly, RNAi dual-luciferase reporter assay cells revealed that, unlike regulated by factor nuclear erythroid 2-related 2 (Nrf2), not subject regulation cap 'n' collar isoform C (CncC), Nrf2 homologue insects. These data provide insights function regulatory mechanism HOs had applied implications for control

Language: Английский

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Duguetia lanceolata A. St.-Hil. (Annonaceae) Essential Oil: Toxicity against Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) and Selectivity for the Parasitoid Trichogramma pretiosum Riley (Hymenoptera: Trichogrammatidae)

Agriculture, Journal Year: 2023, Volume and Issue: 13(2), P. 488 - 488

Published: Feb. 18, 2023

The fall armyworm (FAW) Spodoptera frugiperda is a polyphagous pest that difficult to control due populations resistant various active ingredients. Thus, the objective of this study was evaluate toxicity essential oils (EOs) from organs Annona neolaurifolia, Duguetia lanceolata, and Xylopia brasiliensis, against FAW its natural enemy, Trichogramma pretiosum. most EOs were those leaves stem bark D. which presented LD90 S. equal 70.76 127.14 µg EO/larvae, respectively. major compounds in EO lanceolata (leaves) β-caryophyllene caryophyllene oxide. Although individually inactive FAW, when combined, reduced insect’s probability survival. However, mortality lower than caused by EO. This result suggests other components contribute activity FAW. Furthermore, low egg-larva stage T. pretiosum, but toxic phases. potentially useful for developing new products frugiperda.

Language: Английский

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Genome-Wide Analysis of Detoxification Genes Conferring Diamide Insecticide Resistance in Spodoptera Exigua Identifies Cyp9a40

Published: Jan. 1, 2024

Language: Английский

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The genetic architecture of resistance to flubendiamide insecticides inHelicoverpa armigera(Hübner) (Lepidoptera: Noctuidae)

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 28, 2024

Abstract Insecticide resistance is a major problem in food production, environmental sustainability, and human health. The cotton bollworm Helicoverpa armigera globally distributed crop pest affecting over 300 species. H. has rapidly evolved insecticide resistance, making it one of the most damaging pests worldwide. Understanding genetic basis provides insights to develop tools, such as molecular markers, that can be used slow or prevent evolution resistance. We explore architecture widely insecticide, flubendiamide, using two complementary approaches: genome-wide association studies (GWAS) wild-caught samples quantitative trait locus (QTL) mapping controlled cross susceptible resistant laboratory strains. Both approaches identified on chromosome 2, revealing SNPs within 976 bp monitor field flubendiamide. This was only region linkage mapping, though GWAS revealed additional sites associated with Other loci by populations contained known detoxification genes from ATP-binding cassette family, ABCA1, ABCA3, ABCF2 MDR1. Our findings an oligogenic architecture, contrast previous reports monogenic ryanodine receptor . work elucidates evolving will contribute development effective management strategies. Author summary agricultural challenges security, various classes well Bacillus thuringiensis toxins. this crucial for developing (IRM) study investigated flubendiamide wild-derived QTL backcross One specific 2 found both analysis showed significant potential PCR-based marker monitoring Additionally, five more populations. candidate are primarily mechanisms calcium homeostasis. advances understanding complex armigera, providing valuable tools IRM programs establishing future studies.

Language: Английский

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