Plant Stress, Journal Year: 2024, Volume and Issue: 14, P. 100569 - 100569
Published: Aug. 19, 2024
Language: Английский
Insect Molecular Biology, Journal Year: 2024, Volume and Issue: unknown
Published: July 3, 2024
Abstract We highlight the recent 5 years of research that contributed to our understanding mechanisms RNA interference (RNAi) in insects. Since its first discovery, RNAi has enormously as a reverse genetic tool for functional genomic studies. is also being used therapeutics, well agricultural crop and livestock production protection. Yet, wider application RNAi, improvement potency delivery technologies needed. A mechanistic every step from cellular uptake trigger molecules targeted mRNA degradation, key developing an efficient strategy improve technology. Insects provide excellent model studying mechanism due species‐specific variations efficiency. This allows us perform comparative studies insect species with different sensitivity. Understanding insects can lead development better strategies manage agriculturally medically important
Language: Английский
Citations
7
Journal of Insect Physiology, Journal Year: 2025, Volume and Issue: 161, P. 104754 - 104754
Published: Feb. 9, 2025
Language: Английский
Citations
0
The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 950, P. 175311 - 175311
Published: Aug. 8, 2024
Language: Английский
Citations
3
Comparative Biochemistry and Physiology Part A Molecular & Integrative Physiology, Journal Year: 2024, Volume and Issue: 297, P. 111703 - 111703
Published: July 18, 2024
Language: Английский
Citations
2
Parasites & Vectors, Journal Year: 2023, Volume and Issue: 16(1)
Published: Jan. 18, 2023
More than half of the world's population suffers from epidemic diseases that are spread by mosquitoes. The primary strategy used to stop mosquito-borne is vector control. Interference RNA (RNAi) a powerful tool for controlling insect populations and may be less susceptible resistance other strategies. However, public concerns have been raised because transfer antibiotic marker genes environmental microorganisms after integration into recipient genome, thus allowing pathogen acquire resistance. Therefore, in present study, we modified 3-hydroxykynurenine transaminase (3hkt) hormone receptor 3 (hr3) RNAi vectors remove retain expression cassette inverse repeat sequence 3hkt/hr3 target gene. This recombinant microalgal marker-free insecticide was subsequently added suburban water simulated-field trial test its ability control mosquito population. inverted DNA fragment argininosuccinate lyase gene without ampicillin were obtained using restriction enzyme digestion recovery. After cotransformation Chlamydomonas, algae then employed feed Aedes albopictus larvae. Ten 300 larvae small- large-scale laboratory Ae.albopictus feeding trials, respectively. Simulated field trials conducted Meishe River complemented with Chlamydomonas. Moreover, impact microalgae on phytoplankton zooplankton released explored via high-throughput sequencing. RNAi-recombinant Chlamydomonas effectively silenced gene, resulting inhibition Ae. development also high rate mortality simulated trials. In addition, results confirmed effect plankton similar nontransgenic which could reduce abundance species plankton. highly lethal mosquito, their algal strains, reduces Thus, can biorational disease prevention.
Language: Английский
Citations
4
Journal of Insect Science, Journal Year: 2023, Volume and Issue: 23(4)
Published: July 1, 2023
The Mormon cricket, Anabrus simplex, is a flightless katydid, one of the major devastating rangeland pests in several states western United States. During past few years, their sudden and periodic outbreaks into massive migratory bands caused significant economic losses to forage agricultural crops, particularly grain crops. Current population management methods rely heavily on broad-spectrum chemical insecticides, which could be toxic nontargets, even targeted species might develop resistance long run. Therefore, we assessed potential RNA interference (RNAi)-based alternative strategies that supplement current methods. In insects, RNAi efficiency varies with method double-stranded (dsRNA) delivery. We tested 2 different dsRNA delivery: injection oral feeding dsRNA. results showed crickets are sensitive dose-dependent manner, but refractory Further, confirmed high nuclease activity insect midgut. order protect from dsRNase facilitate its uptake midgut, encapsulated inside poly lactic-co-glycolic acid (PLGA) nanoparticles studied release kinetics by feeding. clearly suggested PLGA nanoparticle permeates digestive system hemolymph; however, it failed induce an efficient response genes. conclusion, our findings suggest responses delivery crickets, further investigations involving stability mechanism required use as cricket strategy.
Language: Английский
Citations
2
Insects, Journal Year: 2024, Volume and Issue: 15(4), P. 292 - 292
Published: April 19, 2024
Basilepta melanopus is a pest that severely affects oil tea plants, and the Notch signaling pathway plays significant role in early development of insect ovaries. In this study, we explored function notch gene within reproductive system B. melanopus. The functional domains expression patterns Bmnotch were analyzed. contains 45 epidermal growth factor-like (EGF-like) domains, one negative regulatory region, NODP domain repeat-containing superfamily. qPCR reveals heightened developmental stages specific tissues like head RNA interference (RNAi)-based suppression decreased its by 52.1%, exhibiting sensitivity to dsNotch at lower concentrations. Phenotypic mating experiments have demonstrated significantly impairs ovarian development, leading reduced frequencies egg production. This decline underscores pathway’s crucial fecundity. findings advocate for RNAi-based, Notch-targeted control as an effective sustainable strategy managing populations, signifying advancement forest endeavors.
Language: Английский
Citations
0
Plant Stress, Journal Year: 2024, Volume and Issue: 14, P. 100569 - 100569
Published: Aug. 19, 2024
Language: Английский
Citations
0