Use of micro and macroalgae extracts for the control of vector mosquitoes DOI Creative Commons
Özge Tufan-Çetin, Hüseyin Çetin

PeerJ, Journal Year: 2023, Volume and Issue: 11, P. e16187 - e16187

Published: Oct. 9, 2023

Mosquitoes are one of the most dangerous vectors human diseases such as malaria, dengue, chikungunya, and Zika virus. Controlling these is a challenging responsibility for public health authorities worldwide. In recent years, use products derived from living organisms has emerged promising approach mosquito control. Among organisms, algae great interest due to their larvicidal properties. Some algal species provide nutritious food larvae, while others produce allelochemicals that toxic larvae. this article, we reviewed existing literature on potential extracts micro- macroalgae, transgenic microalgae, nanoparticles mosquitoes underlying mechanisms. The results many publications show effects macroalgae vary according type extraction, solvents, species, exposure time, larval stage, components. A few studies suggest components have through synergistic interaction between components, inhibition feeding, damage gut membrane cells, digestive detoxification enzymes. conclusion, extracts, shown significant activity against mosquitoes, making them candidates development new control products.

Language: Английский

Changes in the Stress Response and Fitness of Hybrids Between Transgenic Soybean and Wild-Type Plants Under Heat Stress DOI Creative Commons
Li Zhang, Yu Qi, Xin Yin

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(4), P. 622 - 622

Published: Feb. 19, 2025

Understanding the ability of hybrids genetically modified (GM) soybean and wild to survive reproduce under unfavorable conditions is critical for answering questions regarding risk assessment existence transgenes in environment. To investigate effects high-temperature stress on growth competitive ability, GM DBN8002, which expresses VIP3Aa PAT proteins, F2 generations derived from a cross between NJW (wild soybean) were placed greenhouse with an elevated temperature (38/32 °C) 14 days, plant agronomic performance foreign protein levels hybrid evaluated observe their responses high temperature. The results revealed that not influenced by stress. In contrast, pollen germination, pod number, hundred-seed weight, seed vigor parent plants decreased after However, except number fully filled seeds per plant, above parameters similar or slightly lower than those soybean, no significant difference fitness was observed indicating its female heat conditions, resulting persisting spreading within agricultural ecosystems. Our enhance understanding plant's response stress, lay foundation breeding heat-resistant varieties, provide new insights advanced information ecological risks arising escape transgenes.

Language: Английский

Citations

0
Genome editing in the green alga Chlamydomonas: past, present practice and future prospects DOI Creative Commons
Adrian P. Nievergelt

The Plant Journal, Journal Year: 2025, Volume and Issue: 122(1)

Published: April 1, 2025

SUMMARY The green alga Chlamydomonas is an important and versatile model organism for research topics ranging from photosynthesis metabolism, cilia, basal bodies to cellular communication the cycle of significant interest bioengineering processes. genome in this unicellular contained 17 haploid chromosomes codes 16 883 protein coding genes. Functional genomics, as well biotechnological applications, rely on ability remove, add, change these genes a controlled efficient manner. In review, history gene editing put context wider developments genetics demonstrate how many key engineer algae follow global trends availability technology. Building background, overview state art engineering given, focusing primarily practical aspects while giving examples recent applications. Commonly encountered ‐specific challenges, developments, community resources are presented, finally, comprehensive discussion emergence evolution CRISPR/Cas‐based precision given. An outline possible future paths based current genetic tools presented.

Language: Английский

Citations

0
Engineered Gut Symbiotic Bacterium-Mediated RNAi for Effective Control of Anopheles Mosquito Larvae DOI Creative Commons

Jinjin Ding,

Chunlai Cui, Guandong Wang

et al.

Microbiology Spectrum, Journal Year: 2023, Volume and Issue: 11(4)

Published: July 17, 2023

Anopheles mosquitoes are the primary vectors for transmission of malaria parasites, which poses a devastating burden on global public health and welfare. The recent invasion stephensi in Africa has made eradication more challenging due to its outdoor biting behavior widespread resistance insecticides. To address this issue, we developed new approach mosquito larvae control using gut microbiota-mediated RNA interference (RNAi). We engineered symbiotic bacterium, Serratia fonticola, by deleting RNase III gene produce double-stranded RNAs (dsRNAs) larval gut. found that S. fonticola strains can stably colonize guts dsRNAs dsMet or dsEcR activate RNAi effectively suppress expression methoprene-tolerant Met ecdysone receptor EcR, encode receptors juvenile hormone pathways mosquitoes, respectively. Importantly, markedly inhibit development A. leads high mortality, providing an effective dsRNA delivery system silencing genes insects novel RNAi-mediated pest strategy. Collectively, our symbiont-mediated (smRNAi) offers innovative sustainable method controlling provides promising strategy combating malaria. IMPORTANCE Mosquitoes various diseases, imposing significant threat globally. (RNAi) is uses silence specific pests. This study presents use as efficient control. knockout III, dsRNA-specific endonuclease gene, CRISPR-Cas9 led production. Engineered two critical genes, cause mortality larvae. highlights potential exploring microbiota source RNAi-based

Language: Английский

Citations

7
Use of micro and macroalgae extracts for the control of vector mosquitoes DOI Creative Commons
Özge Tufan-Çetin, Hüseyin Çetin

PeerJ, Journal Year: 2023, Volume and Issue: 11, P. e16187 - e16187

Published: Oct. 9, 2023

Mosquitoes are one of the most dangerous vectors human diseases such as malaria, dengue, chikungunya, and Zika virus. Controlling these is a challenging responsibility for public health authorities worldwide. In recent years, use products derived from living organisms has emerged promising approach mosquito control. Among organisms, algae great interest due to their larvicidal properties. Some algal species provide nutritious food larvae, while others produce allelochemicals that toxic larvae. this article, we reviewed existing literature on potential extracts micro- macroalgae, transgenic microalgae, nanoparticles mosquitoes underlying mechanisms. The results many publications show effects macroalgae vary according type extraction, solvents, species, exposure time, larval stage, components. A few studies suggest components have through synergistic interaction between components, inhibition feeding, damage gut membrane cells, digestive detoxification enzymes. conclusion, extracts, shown significant activity against mosquitoes, making them candidates development new control products.

Language: Английский

Citations

5