The black-legged tickIxodes scapularisdetects CO2without the Haller’s organ DOI Creative Commons
Carola Städele

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 5, 2023

ABSTRACT Both male and female ticks have a strong innate drive to find blood-feed on hosts. Carbon dioxide (CO 2 ) is considered critical behavioral activator attractant for an essential sensory cue Yet, how CO activates promotes host-seeking in poorly understood. We studied responses the black-legged tick Ixodes scapularis , primary vector Lyme disease North America. Adult males females were exposed 1, 2, 4, or 8% changes walking behavior foreleg movement analyzed. that potent stimulant adult even at lower concentrations (1%). Behavioral reactions depend animal’s state: Walking increase their speed, while stationary start wave forelegs begin quest – both behaviors resembling aspects of host-seeking. Furthermore, has no clear concentration preference not tuned more robust breath-like (∼4%) than other tested. As soon as level above certain threshold, react, indicating acts can be used long-distance detect approaching Moreover, we provide convincing evidence Haller’s organ necessary detection. Even with disabled amputated organ, respond robustly signifying there must -sensitive structures important yet been identified.

Language: Английский

The black-legged tick Ixodes scapularis detects CO2 without the Haller's organ DOI Creative Commons
Carola Städele

Journal of Experimental Biology, Journal Year: 2024, Volume and Issue: 227(6)

Published: Feb. 19, 2024

Both male and female ticks have a strong innate drive to find blood-feed on hosts. Carbon dioxide (CO2) is considered critical behavioral activator attractant for an essential sensory cue Yet, how CO2 activates promotes host seeking in poorly understood. responses were studied the black-legged tick Ixodes scapularis, primary vector Lyme disease North America. Adult males females exposed 1%, 2%, 4% or 8% CO2, changes walking behavior foreleg movement analyzed. potent stimulant adult I. even at lower concentrations (1%). Behavioral reactions depended animal's state: increased their speed, while stationary started wave forelegs began quest - both behaviors resembling aspects of seeking. Only sporadic cases did animals start walk when supporting hypothesis that acts as rather than attractant. Furthermore, scapularis not show clear concentration preference was tuned more robustly breath-like (∼4%) other tested. Moreover, convincing evidence provided showing Haller's organ necessary detection. Even with disabled amputated organ, responded signifying there must be CO2-sensitive structures important yet been identified.

Language: Английский

Citations

5
Tick chemosensation and implications for novel control strategies DOI Creative Commons
Dandan Zhang

Current Opinion in Insect Science, Journal Year: 2024, Volume and Issue: 65, P. 101249 - 101249

Published: Aug. 6, 2024

Ticks pose a major threat to the health of humans and animals. The use synthetic acaricides repellents has raised concerns potential environmental risks increasing resistance in ticks. This article highlights importance research on tick chemosensation developing novel control agents. It provides review our current understanding chemosensory system proposes using receptor (CR) genes as molecular targets discover releases high-quality genomes provide unprecedented opportunities explore CR gene repertoires. Further functional characterization is necessary identify receptors for key chemical cues signals unravel whether involves ionotropic and/or metabotropic mechanisms.

Language: Английский

Citations

4
The BiteBarrier perimeter: A passive spatial device for tick control and bite prevention DOI Creative Commons

Maria V. Murgia,

Laurie Widder,

Catherine A. Hill

et al.

Current Research in Parasitology and Vector-Borne Diseases, Journal Year: 2025, Volume and Issue: unknown, P. 100246 - 100246

Published: Jan. 1, 2025

Language: Английский

Citations

0
Whole Genome Sequencing Reveals Clade‐Specific Genetic Variation in Blacklegged Ticks DOI Creative Commons
Jacob Cassens, Adela S. Oliva Chávez, Danielle M. Tufts

et al.

Ecology and Evolution, Journal Year: 2025, Volume and Issue: 15(2)

Published: Feb. 1, 2025

ABSTRACT Ticks and tick‐borne pathogens represent the greatest vector‐borne disease threat in United States. Blacklegged ticks are responsible for most human cases, yet burden is unevenly distributed across northern southern Understanding genetic characteristics influencing phenotypic differences tick vectors critical to elucidating disparities pathogen transmission dynamics. Applying evolutionary analyses molecular variation natural populations ecological gradients will help identify signatures of local adaptation, which improve control mitigation strategies. In this study, we performed whole genome nanopore sequencing individual ( n = 1) blacklegged their geographical range (Minnesota, Pennsylvania, Texas) evaluate divergence among populations. Our integrated identified variants associated with numerous biological processes functions that segregated Notably, displayed genes linked xenobiotic detoxification, transmembrane transport, sulfation may underpin key phenotypes dispersal, host associations, vectorial capacity. Nanopore further allowed recovery complete mitochondrial commensal endosymbiont genomes. study provides evidence epidemiologically relevant gene families clades. This report emphasizes need elucidate basis driving conspecific clades

Language: Английский

Citations

0
Niemann–Pick C2 proteins play crucial role in perception of plant volatiles in Tetranychus cinnabarinus DOI

Yuting Wang,

Chenglong Wang,

W. Liu

et al.

Pest Management Science, Journal Year: 2025, Volume and Issue: unknown

Published: May 8, 2025

Abstract BACKGROUND Herbivorous mites perceive odorants through olfactory mechanisms, though the molecular basis of olfaction in these species remains poorly understood. Recent studies have identified Niemann–Pick C2 (NPC2) proteins as odorant carriers peripheral systems insects. Multiple NPC2 genes been discovered spider mite genomes, yet their specific roles function not fully explored. RESULTS Behavioral assays showed that 8 12 tested plant volatiles elicited either repellent or attractive responses Tetranychus cinnabarinus . Two were responsive to stimulation. Recombinant produced, and microscale thermophoresis (MST) revealed binding: TcinNPC2‐2 bound geranylacetone [dissociation constant (Kd) = 3.32 μ m ], TcinNPC2‐3 farnesol (Kd 9.55 ). Knockdown via RNA interference abolished behavioral assays. CONCLUSIONS Mite a number widely documented. NPC2‐2 NPC2‐3 play critical detection farnesol, respectively. This study enhances our understanding processes herbivorous mites, offering insights for development behavior‐targeting agents control. © 2025 Society Chemical Industry.

Language: Английский

Citations

0
Developing attractants and repellents for ticks: promises and challenges DOI

Daniel G Lingeman,

Kenneth L. O’Dell,

Zainulabeuddin Syed

et al.

Current Opinion in Insect Science, Journal Year: 2024, Volume and Issue: 63, P. 101181 - 101181

Published: Feb. 23, 2024

Language: Английский

Citations

3
Involvement of a Microplusin-like Gene (HlonML-1) in the Olfactory Chemosensation of Haemophysalis longicornis: Expression, RNA Silencing, and Behavioral Implications DOI Creative Commons
Jinlin Zhou,

Zhengmao Xu,

Ceyan Kuang

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(11), P. 2269 - 2269

Published: Nov. 8, 2024

The study of tick olfaction is relatively new compared to that insects, and the molecular mechanisms involved remain poorly understood. Despite several potential chemosensory genes identified in multiple species, these are yet be validated through independent functional experiments. In this research, we cloned analyzed a microplusin-like gene, HlonML-1, investigated its role activities

Language: Английский

Citations

0
POLYLACTIC ACID/ZINC OXIDE NANOSTRUCTURED FIBROUS MATERIAL BY NEEDLELESS ONE SOLVENT ELECTROSPINNING FOR PROTECTION AGAINST TICK-BORNE DISEASES DOI

Liga Rampane,

Zane Zelča,

Chaima Salmi

et al.

International Multidisciplinary Scientific GeoConference SGEM ..., Journal Year: 2024, Volume and Issue: 24, P. 69 - 76

Published: Nov. 1, 2024

Ticks (Ixodidae and Argasidae) pose significant health risks as vectors for infectious diseases, with their prevalence expanding across Europe North America. employ sophisticated sensory mechanisms, notably utilizing Haller's organs, to locate sense hosts. These structures detect various cues, including chemosensation, changes in atmospheric CO2 concentration, radiant heat emitted by potential Once a host is detected, landing facilitated electrostatic attraction, accelerating the precise positioning of tick feeding. Reports indicate that ZnO nanoparticles exhibit acaricidal activity. The strong thermal electrical conductivity may effectively impede host-seeking mechanisms. This study focuses on fabricating ZnO-containing nanostructured polylactic acid (PLA) fiber material repellency. In this research, acetone, chosen its low toxicity compatibility natural acaricides, utilized single-solvent system produce nanofibers long-lasting properties. Successful electrospinning acetone-based systems has been limited needle electrospinning. Here, we present method employing needleless fabricate nanoparticle-loaded PLA using pike-type electrode. PLA-ZnO solutions were characterized density, conductivity, viscosity, other parameters, while resulting mats underwent analysis via FTIR, SEM, evaluation conductivity. Optimization spinning parameters critical voltage, collector distance, environmental factors such temperature humidity was conducted. Our demonstrates creation fibers single solvent through Obtained membranes contain 1-3 wt% within an 8-10 matrix solution heightened advancement underscores tick-repellent materials strategies against tick-borne diseases.

Language: Английский

Citations

0
The black-legged tickIxodes scapularisdetects CO2without the Haller’s organ DOI Creative Commons
Carola Städele

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 5, 2023

ABSTRACT Both male and female ticks have a strong innate drive to find blood-feed on hosts. Carbon dioxide (CO 2 ) is considered critical behavioral activator attractant for an essential sensory cue Yet, how CO activates promotes host-seeking in poorly understood. We studied responses the black-legged tick Ixodes scapularis , primary vector Lyme disease North America. Adult males females were exposed 1, 2, 4, or 8% changes walking behavior foreleg movement analyzed. that potent stimulant adult even at lower concentrations (1%). Behavioral reactions depend animal’s state: Walking increase their speed, while stationary start wave forelegs begin quest – both behaviors resembling aspects of host-seeking. Furthermore, has no clear concentration preference not tuned more robust breath-like (∼4%) than other tested. As soon as level above certain threshold, react, indicating acts can be used long-distance detect approaching Moreover, we provide convincing evidence Haller’s organ necessary detection. Even with disabled amputated organ, respond robustly signifying there must -sensitive structures important yet been identified.

Language: Английский

Citations

0