Sulfur-oxidizing symbionts colonize the digestive tract of their Lucinid hosts DOI Creative Commons

Cristina Alcaraz,

Joana Séneca,

Martin Kunert

et al.

The ISME Journal, Journal Year: 2024, Volume and Issue: 18(1)

Published: Jan. 1, 2024

Abstract Like many marine invertebrates, lucinid clams have an intimate relationship with beneficial sulfur-oxidizing bacteria located within specialized gill cells known as bacteriocytes. Most previous research has focused on the symbionts in gills of these (and other) symbiotic bivalves, often assuming that only persistently colonize gills, at least adult stage. We used 16S rRNA gene sequencing and digital polymerase chain reaction symbiont-specific primers targeting soxB foot, mantle, visceral mass, clam Loripes orbiculatus. also fluorescence situ hybridization probes to examine symbiont distribution level whole holobiont. Despite 40 years symbioses, we detected previously unknown populations several organs, including digestive tract. As well-studied tract may be housed host cells. A 14-month starvation experiment without hydrogen sulfide power metabolism caused a larger reduction numbers compared raising possibility are persistent distinct physiology role symbiosis symbionts. Our results highlight unexpectedly complex relationships between their challenge view chemosynthetic restricted hosts.

Language: Английский

Generating prophylactic immunity against arboviruses in vertebrates and invertebrates DOI
Daniel J. Rawle, Leon E. Hugo, Abigail L. Cox

et al.

Nature reviews. Immunology, Journal Year: 2024, Volume and Issue: 24(9), P. 621 - 636

Published: April 3, 2024

Language: Английский

Citations

11
Effects of Microbes on Insect Host Physiology and Behavior Mediated by the Host Immune System DOI Creative Commons
Shan Zhang, Zhengyan Wang,

Qiong Luo

et al.

Insects, Journal Year: 2025, Volume and Issue: 16(1), P. 82 - 82

Published: Jan. 15, 2025

Innate immunity is critical for insects to adjust complicated environments. Studying the insect immune system can aid in identifying novel insecticide targets and provide insights developing pest control strategies. Insects recognize environmental pathogens through pattern recognition receptors, thus activating innate eliminate pathogens. The of primarily comprises cellular humoral immunity. Toll, deficiency, Janus kinase/signal transducers activators transcription are main signaling pathways regulating Nevertheless, increasing research has revealed that activated by microbes also performs non-immune roles while exerting roles, insulin a key role mediating connection between physiological activities. Therefore, this paper first briefly reviews insects, then summarizes relationship growth development, reproduction, pesticide resistance, chemical communication, cell turnover, lifespan, sleep, energy generation their possible underlying mechanisms. Future directions methodologies proposed, aiming into further study on mechanism linking hosts.

Language: Английский

Citations

0
Effects of municipal wastewater effluents on the invertebrate microbiomes of an aquatic-riparian food web DOI Creative Commons
Emilie Diesbourg, Karen A. Kidd, Brittany G. Perrotta

et al.

Environmental Pollution, Journal Year: 2025, Volume and Issue: unknown, P. 125948 - 125948

Published: Feb. 1, 2025

Language: Английский

Citations

0
Medical Potential of Insect Symbionts DOI Creative Commons

Fanglei Fan,

Zhengyan Wang,

Qiong Luo

et al.

Insects, Journal Year: 2025, Volume and Issue: 16(5), P. 457 - 457

Published: April 26, 2025

Insect symbionts and their metabolites are complex diverse gradually becoming an important source of new medical materials. Some culturable from insects produce a variety active compounds with potential. Among them, fatty acids, antibacterial peptides, polyene macrolides, alkaloids, roseoflavin can inhibit the growth human pathogenic bacteria fungi; lipases, yeast killer toxins, reactive oxygen species, pyridines, polyethers, macrotetrolide nactins, macrolides kill parasites; peptides polyketides tumors. However, due to difficulty in culture vitro, targeting specific sites body, limited capability inconsistent clinical research results, adverse reactions on humans, development antibiotic resistance, application insect field remains its infancy. This paper summarizes potential analyzes status quo existing problems application. Possible solutions these also proposed, aim hastening utilization field.

Language: Английский

Citations

0
Differential gene expression in the insect vector Anasa tristis in response to symbiont colonization but not infection with a vectored phytopathogen DOI Creative Commons
Sandra Y. Mendiola, Jason Chen, Ben Lukubye

et al.

Frontiers in Ecology and Evolution, Journal Year: 2024, Volume and Issue: 12

Published: May 22, 2024

Many insects selectively associate with specific microbes in long-term, symbiotic relationships. Maintaining these associations can be vital for the insect hosts’ development, but must also contend potential coinfections from other environment. Fending off microbial threats while maintaining mutualistic has resulted many developing specialized organs to house beneficial microbes. Though locally concentrated organs, symbiont establishment have global consequences insect, including influence over success of coinfecting colonizing host. We use a transcriptomic approach examine how symbiosis between agricultural pest Anasa tristis and bacteria genus Caballeronia affects gene expression within host at large. simultaneously determine whether colonization impacts responses infection plant pathogen Serratia marcescens , which it vectors plants. found that no significant differential was elicited by S. marcescens. This surprising finding given previous work indicating A. clear rapidly compared aposymbiotic individuals. Our results indicate nonsymbiotic tissues differ greatly their expression, particularly following successful colonization. evidence local downregulation immunity upregulation cell communication functions facilitate - symbiosis.

Language: Английский

Citations

2
A methodology to generate aposymbiotic and gnotobiotic Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) DOI
Zhengyan Wang, Qian Liang, Zhiyuan Liu

et al.

Journal of Stored Products Research, Journal Year: 2024, Volume and Issue: 111, P. 102496 - 102496

Published: Dec. 5, 2024

Language: Английский

Citations

2
Gut Bacteria Promote Phosphine Susceptibility of Tribolium castaneum by Aggravating Oxidative Stress and Fitness Costs DOI Creative Commons
Zhengyan Wang, Shan Zhang, Zhiyuan Liu

et al.

Insects, Journal Year: 2023, Volume and Issue: 14(10), P. 815 - 815

Published: Oct. 15, 2023

Knowledge about resistance mechanisms can provide ideas for pesticide management. Although several studies have unveiled the positive or negative impacts of gut microbes on host resistance, minimal research is available regarding association between and phosphine resistance. To explore influence bacteria susceptibility its molecular basis, mortality, fitness, redox responses, immune responses adult Tribolium castaneum were determined when it was challenged by exposure and/or inoculation. Five cultivable excised from a population phosphine-resistant T. castaneum. Among them, only Enterococcus sp. inoculation significantly promoted to phosphine, while any other had no significant effect susceptibility. Furthermore, exposed decreased female fecundity, oxidative stress, suppressed expression activity superoxide dismutase, catalase, peroxidase. In absence also elicited overactive in castaneum, including deficiency Toll signaling pathways dual oxidase–reactive oxygen species system. These results indicate that likely promotes aggravating stress fitness costs.

Language: Английский

Citations

5
Quantitative analysis of trichocysts in Paramecium bursaria following artificial removal and infection with the symbiotic Chlorella variabilis DOI

Hikaru Morita,

Yuuki Kodama

European Journal of Protistology, Journal Year: 2024, Volume and Issue: 95, P. 126115 - 126115

Published: Aug. 1, 2024

Language: Английский

Citations

1
Effects of the Symbiotic Chlorella variabilis on the Host Ciliate Paramecium bursaria Phenotypes DOI Creative Commons
Yuuki Kodama, Masahiro Fujishima

Microorganisms, Journal Year: 2024, Volume and Issue: 12(12), P. 2537 - 2537

Published: Dec. 9, 2024

Paramecium bursaria, a ciliated protist, forms symbiotic relationship with the green alga Chlorella variabilis. This endosymbiotic association is model system for studying establishment of secondary symbiosis and interactions between symbiont its host organisms. Symbiotic algae reside in specialized compartments called perialgal vacuoles (PVs) within cytoplasm, which protect them from digestion by lysosomal fusion. The P. bursaria spp. characterized mutualism, both organisms benefit this association. Furthermore, also influence their phenotypes, algae-free can be obtained through various methods reassociated algae, making it valuable tool endosymbiosis. Recent advancements genomic transcriptomic studies on hosts symbionts have further enhanced utility system. review summarizes infection process C. variabilis effects algal number trichocysts, mitochondria, cytoplasmic crystals, total protein amount, stress responses, photoaccumulation, circadian rhythms bursaria.

Language: Английский

Citations

1
Culex quinquefasciatus Mosquitoes Resist Acquisition of Methicillin-Resistant Staphylococcus aureus: Insights from Field and Laboratory Studies DOI Creative Commons

Waseema Arif,

Gowdham Thangaraj,

Pushpa Srinivasan

et al.

Antibiotics, Journal Year: 2024, Volume and Issue: 13(7), P. 618 - 618

Published: July 2, 2024

The emergence of antimicrobial resistance (AMR) in pathogens and their spillover into the environment have become a global public health menace. Insects can acquire these from would serve as mechanical biological vectors. current study assessed ability Culex quinquefasciatus mosquitoes to methicillin-resistant Staphylococcus aureus (MRSA) through exposure pathogen via rearing water, blood feed, or feeding membrane under laboratory conditions. In addition, mosquito immatures collected habitat vicinity hospitals, veterinary dispensaries, butcher shops at 15 sites Puducherry were screened for MRSA infection. All samples subjected culture-based isolation S. surface homogenate. presence aureus-specific nuc gene genes that confer methicillin (mecA mecC) using PCR tests. was not evident either on external homogenate emerging larvae reared MRSA-spiked water those fed with smeared membranes. Furthermore, any field-caught mosquitoes. Hence, we conclude C. are impervious colonization.

Language: Английский

Citations

1