Knockout BR‐C induces premature expression of E93 thus triggering adult differentiation under larval morphology DOI

Chang Liu,

Mian‐Zhi Wu,

Zhaomin Zheng

et al.

Pest Management Science, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 6, 2024

Abstract BACKGROUND Holometabolan pupal‐specifier broad‐complex ( BR‐C ) and adult specifier ecdysone‐induced protein 93F E93 are essential for metamorphosis; however, their interaction effects on programmed cell death differentiation during pupation remain unclear. RESULTS Here, multiple single‐guide RNA (sgRNA)‐mediated mosaic knockout of induced a deformed larva/pupa intermediate phenotype in Spodoptera frugiperda . Quantitative real‐time polymerase chain reaction (qPCR) analysis showed that the was prematurely expressed mutants penultimate last instar larval stages. Additionally, histological observation TUNEL assay apoptosis fat body midgut activated tissues; astonishingly, appeared pupae mutants. CONCLUSION Overall, results demonstrated premature expression by lack triggers stages, which revealed inhibitory effect metamorphosis S. © 2024 Society Chemical Industry.

Language: Английский

Targeting Autophagy as a Strategy for Developing New Host-Directed Therapeutics Against Nontuberculous Mycobacteria DOI Creative Commons
Jia Wang, Sunhee Lee

Pathogens, Journal Year: 2025, Volume and Issue: 14(5), P. 472 - 472

Published: May 13, 2025

Nontuberculous mycobacteria (NTMs) are increasingly being recognized as opportunistic pathogens in clinical practice because of their innate resistance to antimicrobial treatment and the widespread increase multidrug-resistant strains on a global scale. NTMs pose tremendous infection management challenge, especially individuals with pre-existing lung conditions, well those who immunocompromised. NTMs' capability evade or suppress immune responses host is key feature that makes them cause persistent chronic infection. Autophagy, an essential cellular defense mechanism delivers breaks down intracellular materials lysosomes, protects from mycobacterial Initial studies have revealed encouraging therapeutic strategies augment endogenous autophagic mechanisms block harmful responses, thus having potential decrease infection, including caused by strains. This review discusses how can considers possibilities using autophagy-inducing agents develop novel combat NTM

Language: Английский

Citations

0
The utility of Drosophila melanogaster as a fungal infection model DOI Creative Commons

Chengetai D. Mpamhanga,

Ilias Kounatidis

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: March 14, 2024

Invasive fungal diseases have profound effects upon human health and are on increase globally. The World Health Organization (WHO) in 2022 published the priority list calling for improved public interventions advance research. Drosophila melanogaster presents an excellent model system to dissect host-pathogen interactions has been proved valuable study immunopathogenesis of diseases. In this review we highlight recent advances fungal- interplay with emphasis recently WHO’s focus available tools technologies.

Language: Английский

Citations

1
Atg2 controls Drosophila hematopoiesis through the PVR/TOR signaling pathways DOI
Bo Qin, Hongmei Xue, Xiaoran Wang

et al.

FEBS Journal, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 8, 2024

The hematopoietic system of Drosophila is a well-established genetic model for studying hematopoiesis mechanisms, which are strictly regulated by multiple signaling pathways. Autophagy-related 2 (Atg2) protein involved in autophagosome formation through its lipid transfer function; however, other functions animal development, especially the role Atg2 maintaining homeostasis, unclear. Here, we show that knockdown cortical zone (CZ) induced proliferation and differentiation mature plasmatocytes disrupted progenitor maintenance medullary (MZ). We also observed lamellocytes among circulating hemocytes lymph gland, rarely healthy larvae. above results on disorders due to regulating PDGF/VEGF receptor (PVR) target rapamycin (TOR) CZ gland. In conclusion, identified as previously undescribed regulator hematopoiesis. Understanding mechanism homeostasis will help us better evaluate human blood disorder-related diseases.

Language: Английский

Citations

1
Knockout BR‐C induces premature expression of E93 thus triggering adult differentiation under larval morphology DOI

Chang Liu,

Mian‐Zhi Wu,

Zhaomin Zheng

et al.

Pest Management Science, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 6, 2024

Abstract BACKGROUND Holometabolan pupal‐specifier broad‐complex ( BR‐C ) and adult specifier ecdysone‐induced protein 93F E93 are essential for metamorphosis; however, their interaction effects on programmed cell death differentiation during pupation remain unclear. RESULTS Here, multiple single‐guide RNA (sgRNA)‐mediated mosaic knockout of induced a deformed larva/pupa intermediate phenotype in Spodoptera frugiperda . Quantitative real‐time polymerase chain reaction (qPCR) analysis showed that the was prematurely expressed mutants penultimate last instar larval stages. Additionally, histological observation TUNEL assay apoptosis fat body midgut activated tissues; astonishingly, appeared pupae mutants. CONCLUSION Overall, results demonstrated premature expression by lack triggers stages, which revealed inhibitory effect metamorphosis S. © 2024 Society Chemical Industry.

Language: Английский

Citations

0