Salivary cytokine profile in patients with ischemic stroke

Scientific Reports, Journal Year: 2021, Volume and Issue: 11(1)

Published: Aug. 25, 2021

Inflammation plays a crucial role in stroke pathogenesis. Thus, it is not surprising that cytokines, chemokines, and growth factors have been advocated diagnostics. Our study the first to evaluate salivary cytokine profile patients with ischemic stroke. Twenty-five subacute an age-, sex-, oral hygiene status-matched control group were enrolled study. The number of was set priori based on our previous experiment (α = 0.05, test power 0.9). Salivary concentrations tumor necrosis factor α (TNF-α), interleukin 6 (IL-6), 10 (IL-10) assessed using ELISA method. We showed TNF-α IL-6 significantly higher, whereas IL-10 content statistically lower both non-stimulated (NWS) stimulated (SWS) whole saliva patients. However, evaluation cytokines NWS rather than SWS may be greater diagnostic value. Of particular note TNF-α, which indicate cognitive/physical impairment post-stroke individuals. This parameter distinguishes from healthy controls correlates cognitive decline severity functional impairment. It also differentiates (with high sensitivity specificity) normal cognition mild moderate Saliva alternative blood for assessing patients, although further studies larger patient population are needed.

Language: Английский

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Salivary Gland Dysfunction in Patients with Chronic Heart Failure Is Aggravated by Nitrosative Stress, as Well as Oxidation and Glycation of Proteins

Biomolecules, Journal Year: 2021, Volume and Issue: 11(1), P. 119 - 119

Published: Jan. 18, 2021

Chronic heart failure (HF) is an important clinical, social, and economic problem. A key role in HF progression played by oxidative stress. Free oxygen radicals, formed under the conditions of hypoxia reperfusion, participate myocardial stunning other forms post-reperfusion damage. patients also suffer from disorders connected with saliva secretion. However, still little known about mechanisms that impair secretory function salivary glands these patients. In presented study, we were first to compare antioxidant barrier, protein glycoxidation, nitrosative/nitrative stress non-stimulated (non-stimulated whole (NWS)) stimulated (SWS) The study included 50 normal (NS) secretion (n = 27) hyposalivation (HS) 23), as well age- gender-matched control group 50). We demonstrated that, NWS HS, concentration low-molecular-weight non-enzymatic antioxidants decreased (↓total polyphenols, ↓ascorbic acid, ↓reduced glutathione, ↓albumin) compared secretion, (except albumin). observed increased content glycoxidation products (↑dityrosine, ↑kynurenine, ↑glycophore) SWS HS healthy controls. Interestingly, dityrosine, N-formylkynurenine, glycophore was significantly higher those NS NO considerably lower, while levels peroxynitrite nitrotyrosine subjects Salivary gland dysfunction occurs chronic submandibular being least efficient. Oxidative/nitrosative may be one responsible for impairment

Language: Английский

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Enzymatic antioxidants activity in gingival crevicular fluid and saliva in advanced periodontitis

Oral Diseases, Journal Year: 2022, Volume and Issue: 29(8), P. 3559 - 3570

Published: June 21, 2022

Enzymatic antioxidants are the primary line of defense against oxidative and nitrosative stress. However, their involvement in progression periodontitis is still not well understood. The study aimed to determine activity enzymatic gingival crevicular fluid (GCF) saliva patients with periodontitis.

Language: Английский

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The Link between Stroke Risk and Orodental Status—A Comprehensive Review

Journal of Clinical Medicine, Journal Year: 2022, Volume and Issue: 11(19), P. 5854 - 5854

Published: Oct. 2, 2022

One of the primary causes disability and mortality in adult population worldwide is stroke. A person’s general health significantly impacted by their oral dental health. People who have poor are more susceptible to conditions such as Stroke risk has long been linked conditions. The stroke its cost impact on healthcare systems appear be reduced a result decline incidence prevalence illnesses. Hypothetically, better management hygiene lead risk. To authors’ best knowledge, for first time, potential link between were cross-examined. most typical symptoms, illnesses stroke, role professionals prevention revealed. mediating processes subsequent long-term cognitive functional neurological outcomes based available literature. It must noted that periodontal diseases tooth loss two common measures. Lack knowledge effects systemic together with limited access medical or care considered partially responsible elevated Concrete evidence confirming associations inflammatory large cohort prospective studies, stratifying association disease severity survival will desirable. In terms clinical pathology, predictive model function status, biomarkers inflammation could useful both cardiologists dentists.

Language: Английский

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Cytotoxicity of Acrylic Resins, Particulate Filler Composite Resin and Thermoplastic Material in Artificial Saliva with and without Melatonin

Materials, Journal Year: 2022, Volume and Issue: 15(4), P. 1457 - 1457

Published: Feb. 16, 2022

There is limited information on the effect of melatonin cytotoxicity dental materials. The study evaluated cytotoxic effects heat- and auto-polymerized acrylic resin, particulate filler composite resin a thermoplastic material L-929 fibroblast cell viability at different incubation periods in artificial saliva without with melatonin. Disk-shaped specimens were prepared according to each manufacturer's instructions divided into two groups be stored either (AS) AS (ASM). measurements performed using an MTT (3-(4,5)-dimethylthiazol-2-yl)-2,5-diphenyl tetrazoliumbromide) assay, which mouse fibroblasts culture was used. For test, extracts examined 1, 24, 72 h 1 2 weeks. Data analyzed 3-way ANOVA Tukey's tests. No significant difference found between ASM (F = 0.796; p 0.373). Incubation period significantly affected all materials tested (p < 0.001). Storing resin-based solution for 24 may reduce cells highest observed. Soaking prosthesis or orthodontic appliances least before intraoral use rinsing medium containing recommended decreasing

Language: Английский

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