Expansion and Delivery of Human Chondrocytes on Gelatin-Based Cell Carriers

Gels, Journal Year: 2025, Volume and Issue: 11(3), P. 199 - 199

Published: March 13, 2025

Cartilage damage is common in sports injuries and cartilage-related diseases, such as degenerative joint rheumatic disorders. Autologous chondrocyte implantation (ACI) a widely used cell-based therapy for repairing cartilage clinical practice. In this procedure, patient’s chondrocytes are isolated, cultured vitro to expand the cell population, then implanted into damaged site. However, expansion of on standard 2D culture surfaces leads dedifferentiation (loss phenotype), delivery detached cells has proven be ineffective. To overcome these limitations, matrix-assisted ACI (MACI) procedure was developed. MACI, matrices hydrogels microspheres carriers or scaffolds deliver expanded chondrocytes, enhancing viability precision delivery. streamline two key steps MACI—cell delivery—this study aims investigate various configurations gelatin-based their potential support both single step. This evaluated gelatin (Gel MS), micronized photo-crosslinked GelMA microparticles (GelMA MP), bulky containing HG). Cell growth, maintenance phenotype, extracellular matrix (ECM) production were assessed pellet cultures grown on/in carriers, compared with tissue culture-treated polystyrene (TCP). Our results demonstrate that normal human knee articular exhibit robust growth Gel MS form aggregates enriched glycosaminoglycan-rich ECM. outperformed MP HG carrier by supporting long-term reduced

Language: Английский

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Photocrosslinkable Biomaterials for 3D Bioprinting: Mechanisms, Recent Advances, and Future Prospects

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(23), P. 12567 - 12567

Published: Nov. 22, 2024

Constructing scaffolds with the desired structures and functions is one of main goals tissue engineering. Three-dimensional (3D) bioprinting a promising technology that enables personalized fabrication devices regulated biological mechanical characteristics similar to natural tissues/organs. To date, 3D has been widely explored for biomedical applications like engineering, drug delivery, screening, in vitro disease model construction. Among different bioinks, photocrosslinkable bioinks have emerged as powerful choice advanced devices, fast crosslinking speed, high resolution, great print fidelity. The biomaterials used light-based printing play pivotal role functional constructs. Herein, this review outlines general approaches related biomaterials, including extrusion-based printing, inkjet stereolithography laser-assisted printing. Further, mechanisms, advantages, limitations photopolymerization photoinitiators are discussed. Next, recent advances synthetic highlighted. Finally, challenges future perspectives envisaged.

Language: Английский

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Gelatin methacryloyl bioinks for bioprinting nasal cartilage: Balancing mechanical integrity and extracellular matrix formation

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 143559 - 143559

Published: April 1, 2025

Language: Английский

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Screening of MMP-13 Inhibitors Using a GelMA-Alginate Interpenetrating Network Hydrogel-Based Model Mimicking Cytokine-Induced Key Features of Osteoarthritis In Vitro

Polymers, Journal Year: 2024, Volume and Issue: 16(11), P. 1572 - 1572

Published: June 1, 2024

Osteoarthritis (OA) is a chronic joint disease characterized by irreversible cartilage degradation. Current clinical treatment options lack effective pharmaceutical interventions targeting the disease’s root causes. MMP (matrix metalloproteinase) inhibitors represent new approach to slowing OA progression addressing degradation mechanisms. However, very few drugs within this class are in preclinical or trial phases. Hydrogel-based 3D vitro models have shown promise as testing platforms due their resemblance native extracellular matrix (ECM), abundant availability, and ease of use. Metalloproteinase-13 (MMP-13) thought be major contributor articular aggressively breaking down type II collagen. This study focused on MMP-13 using GelMA-alginate hydrogel-based model induced cytokines interleukin-1 beta (IL-1β) tumor necrosis factor alpha (TNF-α). The results demonstrate significant inhibition collagen breakdown measuring C2C concentration ELISA after with inhibitors. inconsistencies human explant samples led inconclusive results. Nonetheless, highlights an alternative human-sourced explants for drug screening.

Language: Английский

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Comparative studies of GelMA hydrogels: investigating the effect of different source on mechanical, physical and biological properties

Materials Research Express, Journal Year: 2024, Volume and Issue: 11(7), P. 075307 - 075307

Published: July 1, 2024

Abstract GelMA hydrogels are prominent in biomedical applications due to their innate extracellular matrix mimicking properties. They exhibit favorable properties for cell proliferation and formation of light-induced hydrophilic cross-linked structures. However, there is limited research on the effect variations starting material (gelatin) physical, mechanical biological In this study, Gelatin Methacrylic Anhydride (GelMA) were synthesized from two different products type B gelatin loaded with polyvinylpyrrolidone (PVP) nanoparticles by electrospray method. Chemical structural analyses performed FTIR, 1 HNMR, TNBS SEM, respectively. Mechanical evaluated compression tests. Cytocompatibility was XTT analysis. obtained brands have suitable pore size, strength, swelling cytocompatibility, making them various applications. addition, addition PVP can make useful drug delivery

Language: Английский

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