Arcopilins: A New Family of Staphylococcus aureus Biofilm Disruptors from the Soil Fungus Arcopilus navicularis DOI Creative Commons
Esteban Charria‐Girón,

Haoxuan Zeng,

Tatiana E. Gorelik

et al.

Journal of Medicinal Chemistry, Journal Year: 2024, Volume and Issue: 67(17), P. 15029 - 15040

Published: Aug. 14, 2024

Biofilms represent a key challenge in the treatment of microbial infections; for instance, Staphylococcus aureus causes chronic or fatal infections by forming biofilms on medical devices. Herein, fungus Arcopilus navicularis was found to produce novel family PKS-NRPS metabolites that are able disrupt preformed S. aureus. Arcopilins A–F (1–6), tetramic acids, and arcopilin G (7), 2-pyridone, were elucidated using HR-ESI-MS one-dimensional (1D) two-dimensional (2D) nuclear magnetic resonance (NMR) spectroscopy. Their absolute configuration established synthesis MPTA-esters 2, analysis 1H–1H coupling constants, ROESY correlations, along with comparison crystal structure 7. Arcopilin A (1) not only effectively disrupts but also potentiates activity gentamicin vancomycin up 115- 31-fold times, respectively. Our findings demonstrate potential application arcopilins conjugated caused antibiotics unable biofilms.

Language: Английский

Serine Hydrolase-Catalyzed Polyol Lipids are Necessary for Rodlet Layer Formation on the Cell Wall of Entomopathogenic Fungi DOI
Dekun Kong, He Qian,

Dongmei Lin

et al.

Journal of the American Chemical Society, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 30, 2025

Some key secondary metabolism genes are important for driving the infection process of entomopathogenic fungi; however, their chemical substance basis has not been well investigated. Here, mixtures polyol lipids discovered, which synthesized through iterative chain transfer-esterification-hydrolysis cycles catalyzed by serine hydrolase during release online highly reducing polyketide intermediates. Importantly, an in vivo gene knockout experiment revealed that synthesis is necessary rodlet layer formation on cell wall Beauveria bassiana. Our work uncovers unexpected way and illuminates a new perspective part significant physiological processes fungi.

Language: Английский

Citations

1
Comparing total chemical synthesis and total biosynthesis routes to fungal specialized metabolites DOI Creative Commons
Dong-Song Tian, Xiao Zhang, Russell J. Cox

et al.

Natural Product Reports, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Here we compare chemical and biological total syntheses to the same metabolites.

Language: Английский

Citations

4
Antibiofilm and cytotoxic metabolites from the entomopathogenic fungus Samsoniella aurantia DOI Creative Commons
Rita Toshe, Syeda J. Khalid, Blondelle Matio Kemkuignou

et al.

Beilstein Journal of Organic Chemistry, Journal Year: 2025, Volume and Issue: 21, P. 327 - 339

Published: Feb. 11, 2025

During the course of our studies on secondary metabolism rare, hitherto untapped Thai insect-associated fungi, ethyl acetate (EtOAc) extract derived from solid-state cultivation Samsoniella aurantia rice afforded one previously undescribed tetramic acid derivative, farinosone D ( 1 ), along with known 2-pyridones, farinosones A 2 ) and B 3 cyclodepsipeptides beauvericins A–C 4 – 6 ). All isolated compounds were assessed for their antimicrobial cytotoxic activities while selected biofilm inhibitory activity assay. Farinosone showed significant IC 50 values in low micromolar to nanomolar range against several mammalian cell lines. On other hand, which lacked potent effects, revealed antibiofilm activity, inhibiting approximately 70% Staphylococcus aureus biofilms at concentrations as 3.9 µg/mL.

Language: Английский

Citations

0
Arcopilins: A New Family of Staphylococcus aureus Biofilm Disruptors from the Soil Fungus Arcopilus navicularis DOI Creative Commons
Esteban Charria‐Girón,

Haoxuan Zeng,

Tatiana E. Gorelik

et al.

Journal of Medicinal Chemistry, Journal Year: 2024, Volume and Issue: 67(17), P. 15029 - 15040

Published: Aug. 14, 2024

Biofilms represent a key challenge in the treatment of microbial infections; for instance, Staphylococcus aureus causes chronic or fatal infections by forming biofilms on medical devices. Herein, fungus Arcopilus navicularis was found to produce novel family PKS-NRPS metabolites that are able disrupt preformed S. aureus. Arcopilins A–F (1–6), tetramic acids, and arcopilin G (7), 2-pyridone, were elucidated using HR-ESI-MS one-dimensional (1D) two-dimensional (2D) nuclear magnetic resonance (NMR) spectroscopy. Their absolute configuration established synthesis MPTA-esters 2, analysis 1H–1H coupling constants, ROESY correlations, along with comparison crystal structure 7. Arcopilin A (1) not only effectively disrupts but also potentiates activity gentamicin vancomycin up 115- 31-fold times, respectively. Our findings demonstrate potential application arcopilins conjugated caused antibiotics unable biofilms.

Language: Английский

Citations

1