Newly isolated Trichoderma spp. show multifaceted biocontrol strategies to inhibit potato late blight causal agent Phytophthora infestans both in vitro and in planta

Phytobiomes Journal, Journal Year: 2023, Volume and Issue: 8(1), P. 70 - 84

Published: March 14, 2023

Potato growers worldwide have been at war for more than 150 years with an enemy whose lifecycle, genome size and architecture, infection rate, economic impacts are the epitome of a plant pathogen. Phytophthora infestans is oomycete that causes notorious late blight in potato tomato fields. This study explored benefits multitalented symbiotic fungi Trichoderma spp. their metabolites as potential biopesticides against P. infestans. Eleven strains were obtained from soil tree barks identified using DNA sequence analysis three molecular markers. The antagonistic was first evaluated vitro. In dual-culture assays, growth significantly inhibited (53 to 95%) by different through direct mycoparasitism, competition space nutrients, or antibiosis. cell-free filtrates (CFFs) characterized anti- activities well biochemical stability. results indicated CFFs chemically stable strongly decreased infestans’ mycelial zoospore motility viability. Similarly, leaf-disk showed significant protection infection. Ultraperformance liquid chromatography revealed presence harzianic acid, iso-harzianic 6-pentyl-2H-pyran-2-one major compounds CFFs. Furthermore, selected protected plants soil-mediated Finally, high compatibility copper-based fungicide, especially lower concentrations, suggesting both protective agents could be combined integrated pest management programs. [Formula: see text] Copyright © 2024 Author(s). open access article distributed under CC BY-NC-ND 4.0 International license .

Language: Английский

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Isolation and Characterization of Trichoderma spp. for Antagonistic Activity against Avocado (Persea americana Mill) Fruit Pathogens

Horticulturae, Journal Year: 2022, Volume and Issue: 8(8), P. 714 - 714

Published: Aug. 9, 2022

In this research, we aimed to isolate and identify native strains of Trichoderma spp. with potential activity against avocado pathogens (Neofusicoccum parvum, Colletotrichum gloeosporioides, Diaporthe sp., Phomopsis perseae). Strains were isolated from roots soil obtained orchards different regions Mexico. Twenty-five isolated, which six (TSMICH7, TSMICH8, TRMICH9, TSMICH10, TSMICH15, TRJAL25) showed greater antagonistic capacity in vitro (>80%) pathogens. After 96 h, the strain undergoes a thickening hyphae, while phytopathogen tends thin, except for thicken. The characterization these was carried out through morphological observations amplification sequencing rDNA fragments (ITS regions), as well translation elongation factor 1-alpha (Tef 1-α), achieving identification harzianum. However, vivo evaluation (applying directly fruit), TSMICH7 maintained considerably high effectiveness (>90%) four phytopathogens tested, mainly P. perseae, N. increasing glucanases chitinases. Therefore, T. harzianum could be used biological control agent inhibit post-harvest avocados, thus avoiding significant losses fruit international importance.

Language: Английский

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The interaction of the pathogen Fusarium proliferatum with Trichoderma asperellum characterized by transcriptome changes in apple rootstock roots

Physiological and Molecular Plant Pathology, Journal Year: 2022, Volume and Issue: 121, P. 101894 - 101894

Published: Aug. 28, 2022

Language: Английский

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Allium fistulosum L. Alleviates Apple Replant Disease by Suppressing Fusarium solani

Journal of Fungi, Journal Year: 2022, Volume and Issue: 8(10), P. 1071 - 1071

Published: Oct. 12, 2022

Fusarium solani has often been isolated from replanted apple roots, suggesting that it is associated with replant disease. The mechanism underlying the ability of mixed cropping trees Allium fistulosum L. to alleviate disease remains unclear. aim this study was determine pathogenicity isolate HBH 08 diseased roots and effect A. its root secretions on seedings. field experiment showed not only significantly reduced amount in soil but also increased biomass grafted seedlings. GC-MS analysis indicated dimethyl disulphide diallyl were active molecules exudates They inhibited growth mycelium decreased number spores germinated. In addition, these compounds under conditions promoted Overall, might be an effective approach for cultivating controlling

Language: Английский

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Microbial endophytes as probiotics for the plant health: an overview

Elsevier eBooks, Journal Year: 2022, Volume and Issue: unknown, P. 269 - 281

Published: Nov. 25, 2022

Language: Английский

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