Soil Microbial Communities and Wine Terroir: Research Gaps and Data Needs DOI Creative Commons

Gabriela Crystal Franco,

Jasmine Leiva,

Sanjiev Nand

et al.

Foods, Journal Year: 2024, Volume and Issue: 13(16), P. 2475 - 2475

Published: Aug. 6, 2024

Microbes found in soil can have a significant impact on the taste and quality of wine, also referred to as wine terroir. To date, terroir has been thought be associated with physical chemical characteristics soil. However, there is fragmented understanding contribution vineyard microbes Additionally, vineyards play an important role carbon sequestration since promotion healthy microbial communities directly impacts greenhouse gas emissions atmosphere. We review 24 studies that explore their influence grapevine health, grape composition, quality. Studies spanning 2015 2018 laid foundation by exploring biogeography vineyards, management effects, reservoir function for grape-associated microbiota. On other hand, 2019 2023 appear more specific targeted approach, delving into relationships between metabolites, distribution at different depths, influences flavor composition. Next, we identify research gaps make recommendations future work. Specifically, most utilize sequencing (16S, 26S, ITS), which only reveals community Utilizing high-throughput omics approaches such shotgun (to infer function) transcriptomics (for actual vital determining mechanisms chemistry. Going forward, long-term effects practices climate change microbiology, trunk diseases, bacteriophages wine-making would fruitful investigation. Overall, presented shed light importance microbiomes interactions grapevines shaping production. are still many aspects this complex ecosystem require further exploration support sustainable viticulture enhance

Language: Английский

Optical imaging combined with artificial intelligence in plant disease detection: a comprehensive review DOI
Wei Zhuo,

Yixue Jiang,

Hongtao Liu

et al.

Spectroscopy Letters, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 25

Published: Feb. 23, 2025

Language: Английский

Citations

1
Effect of rootstock diversity and grafted varieties on the structure and composition of the grapevine root mycobiome DOI
Pierre‐Antoine Noceto,

Agnès Mathé,

Laurent Anginot

et al.

Plant and Soil, Journal Year: 2024, Volume and Issue: unknown

Published: March 26, 2024

Language: Английский

Citations

6
Soil Microbial Communities and Wine Terroir: Research Gaps and Data Needs DOI Open Access

Gabriela Crystal Franco,

Jasmine Leiva,

Sanjiev Nand

et al.

Published: June 26, 2024

Microbes found in soil can have a significant impact on the taste and quality of wine, also referred to as wine terroir. To date, terroir has been thought be associated with physical chemical characteristics soil. However, there is fragmented understanding contribution vineyard microbes Additionally, vineyards play an important role carbon sequestration, since promotion healthy microbial communities directly greenhouse gas emissions atmosphere. We review 24 studies that explore their influence grapevine health, grape composition, quality. Studies spanning 2015 2018 laid foundation by exploring biogeography vineyards, management effects, reservoir function for grape-associated microbiota. On other hand, 2019 2023 appear more specific targeted approach, delving into relationships between metabolites, distribution at different depths, influences flavor composition. Next, we identify research gaps make recommendations future work. Specifically, most utilize sequencing (16S, 26S, ITS) which only reveals community Utilizing high-throughput omics approaches such shotgun (to infer function) transcriptomics (for actual vital determine mechanisms chemistry. Going forward, long-term effects practices climate change microbiology, trunk diseases, bacteriophages making would fruitful investigation. Overall, presented shed light importance microbiomes interactions grapevines shaping production. are still many aspects this complex ecosystem require further exploration support sustainable viticulture enhance

Language: Английский

Citations

5
In Vitro Evaluation of Some Endophytic Bacillus to Potentially Inhibit Grape and Grapevine Fungal Pathogens DOI Creative Commons
Oana-Alina Boiu-Sicuia, Radu Cristian Toma, Camelia Diguță

et al.

Plants, Journal Year: 2023, Volume and Issue: 12(13), P. 2553 - 2553

Published: July 5, 2023

Romania has a long history of grapevine culturing and winemaking. However, like any agricultural sector, viticulture faces devastating biological threats. Fungi responsible for trunk diseases (GTDs) grape spoilage lead to considerable yield losses decline in quality. In the actual context, many countries, including Romania, have reoriented their approaches minimize chemical inputs, which been proven be toxic negative impacts on environment, replace them with sustainable biocontrol strategies wine-growing sector. Within strategies, Bacillus spp. is well-known plant-protective bacteria antifungal properties. this paper, six endophytic from various plant sources were studied. The bacterial strains identified as B. pumilus, subtilis, velezensis by sequencing 16S rDNA region. Regardless vitro test methods (using living cells, bacterial-cell-free supernatant (CFS), volatile active compounds (VOCs)), revealed strong broad activity against fungal pathogens such Aspergillus spp., Botrytis cinerea, Penicillium expansum, Diplodia seriata, Eutypa lata, Fusarium Clonostachys rosea, Neofusicoccum parvum, Stereum hirsutum. functional genes encoding difficidin, fengycin, iturins, macrolactin, mycosubtilin molecularly detected, could support strains. Lytic enzymes involved growth inhibition, chitinase, cellulase, proteases, also produced some these Various other tests, phosphate phytate solubilization, phytohormone synthesis, production polyamine biosynthetic pathway, pH well temperature tolerance tests carried out reveal plant-beneficial potential These results that strains, especially BAHs1, are most suitable endophytes biologic control, future development management strategies.

Language: Английский

Citations

12
Health Status and Disinfection Prior to Grafting Affect the Phenolic Profile of Grapevine Hetero-Grafts and Grafting Yield DOI Creative Commons
Saša Gačnik, Maja Mikulič-Petkovšek,

Domen Kjuder

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(3), P. 444 - 444

Published: Feb. 3, 2025

Grapevine trunk disease (GTD) is a major threat to grapevine propagation, severely affecting the growth and development of young vines. As one most destructive plant diseases in world, GTD spreads easily through propagation material threatens sustainability vineyards. While effective, biologically friendly treatments remain unavailable. This study investigated graft yield, potential hetero-grafts, phenolic responses focusing on (i) scion health status (healthy—HLT; asymptomatic—ASYM; symptomatic—SYM) (ii) disinfection methods. Grafting with HLT scions achieved highest yield rates, particularly Serenade® ASO (75%) BioAction ES (79%), while infected showed lower yields. The was not affected by method or scions. Phenolic composition varied between scions, callus, rootstock canes, roots, strongly influencing metabolites. Higher levels flavanols were observed treated ASO, these resulting 1.6 1.5 times higher procyanidin dimer levels, respectively, compared Beltanol. Flavanols stilbenes callus tissue grafts healthy Rootstock also catechin dimers These results indicate that methods significantly influence composition, providing valuable insights for management.

Language: Английский

Citations

0
Native Bacteria Are Effective Biocontrol Agents at a Wide Range of Temperatures of Neofusicoccum parvum, Associated with Botryosphaeria Dieback on Grapevine DOI Creative Commons
Diyanira Castillo-Novales,

Paulina Vega-Celedón,

Alejandra Larach

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(7), P. 1043 - 1043

Published: March 27, 2025

Botryosphaeria dieback, a significant grapevine trunk disease (GTD) caused by various pathogens, represents serious threat to viticulture. Biocontrol emerges as promising sustainable alternative chemical control, aligning toward environmentally friendly viticultural practices. This study evaluated the in vitro, vivo, and situ biocontrol potential of Chilean native bacteria isolated from wild flora endophytic communities against Neofusicoccum parvum. In vitro assays screened 15 bacterial strains at 10, 22, 30 °C, identifying four Pseudomonas with >30% mycelial growth inhibition. diffusible agar double plate assays, plant growth-promoting AMCR2b GcR15a, which were flora, achieved inhibition N. parvum growth, reductions up ~50% (diffusible agar) ~46% (double plate). vivo experiments on cuttings revealed that GcR15a inhibited (17-90%); younger grapevines (1-5 years) more susceptible trials using Vitis vinifera L. cv. Cabernet Sauvignon Blanc demonstrated higher fungal susceptibility Blanc. These results highlight sp. be effective agents GTDs wide range temperatures, contributing

Language: Английский

Citations

0
Nested PCR methodology for detection of Lasiodiplodia sp. and Ceratocystis sp. in avocado and cocoa seedling samples DOI

Laura Valentina Laverde-Arias,

Yeirme Yaneth Jaimes Suárez, Adriana González

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: April 24, 2025

Abstract Background: Persea americana and Theobroma cacao plants can be infected by fungal species of the genus Lasiodiplodia, which cause disease known as dieback. However, T. also Ceratocystis sp., causes cocoa wilt. These fungi necrosis vascular tissue, leading to wilting death plant. these symptoms are not observed in early stages infection, making timely diagnosis pathogens difficult. Therefore, this study, a nested PCR technique was developed for detection sp. Lasiodiplodia sp. from asymptomatic seedling stem samples. Results: A standardized pre-treatment with glycerol 96% ethanol soften avocado tissue effective before DNA isolation. Subsequent amplification actin gene pre-treated tissues confirmed that step is necessary obtain nucleic acids free inhibitors. Furthermore, Cer.ITS119-F/Cer.ITS379-R Las.ITS77-F/Las.ITS452-R primer sets designed study demonstrated specific Ceratocystissp. sp., respectively, sensitivity ranging 2-4 ng/μL. By using primers ITS1/ITS4 Ceratocystis ITS1/ITS4, Lasiodiplodiasp. nested-PCR, both were successfully detected directly samples without isolation culture media. The results easily interpreted agarose electrophoresis according fragment size differences, 279 bp amplicon artificially C. cacaofunesta 397 L. theobromae subglobosa. Conclusion: The use protocol Ceratocystissp.; allowed seedlings.

Language: Английский

Citations

0
Kalmusia variispora (Didymosphaeriaceae, Dothideomycetes) Associated with the Grapevine Trunk Disease Complex in Cyprus DOI Creative Commons
Georgios Makris, Marcelo Sandoval‐Denis, P.W. Crous

et al.

Pathogens, Journal Year: 2025, Volume and Issue: 14(5), P. 428 - 428

Published: April 28, 2025

Grapevine trunk diseases (GTDs) are widespread worldwide, causing serious economic losses to the vitiviniculture industry. The etiology of complex pathogenic mycobiome associated with this group is critical implementing appropriate management strategies. Diseased grapevines exhibiting typical GTD symptoms were collected from vineyards in different provinces Cyprus, resulting 19 pycnidial isolates. A subsequent multilocus sequence analysis six genetic loci (ITS, LSU, SSU, b-tub, tef1-a, and rpb2) identified them as Kalmusia variispora, twelve representative isolates included phylogenetic analyses. According pathogenicity trials on two-year-old potted vines (cv. Mavro), all tested pathogenic, light dark brown discoloration lesions varying levels, ranging 4 12.3 cm long. capacity K. variispora produce cell-wall-degrading exoenzymes was qualitatively estimated solid media. Cellulase, pectinase, laccase production evident for isolates, except isolate CBS 151329, where latter enzyme undetected. severity consistent laccase-producing capacity. present study confirmed association a pathogen represents first description ascomycete causal agent Cyprus. This highly virulent species may play significant role complex, its biological cycle epidemiology should be further investigated.

Language: Английский

Citations

0
Diplodia seriata Biocontrol Is Altered via Temperature and the Control of Bacteria DOI Creative Commons

Alejandra Larach,

Paulina Vega-Celedón,

Diyanira Castillo-Novales

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(2), P. 350 - 350

Published: Feb. 8, 2024

Grapevine trunk diseases (GTDs) attack the vine’s wood, devastating vineyards worldwide. Chile is world’s fourth-largest wine exporter, and Cabernet Sauvignon one of most economically important red varieties. Botryosphaeria dieback an GTD, Diplodia seriata main pathogenic species. Biocontrol studies these pathogens are commonly carried out at different incubation times but a single temperature. This study aimed to evaluate biocontrol effect Chilean PGPB grapevine endophytic bacteria against D. temperatures. We analyzed Pseudomonas sp. GcR15a, AMCR2b Rhodococcus PU4, with three isolates (PUCV 2120, PUCV 2142 2183) 8, 22 35 °C. Two dual-culture antagonism methods (agar plug diffusion double plate) were used in vitro effect, vivo test was performed cuttings. In vitro, greatest inhibitions obtained using agar method temperature 8 °C, where PU4 obtains 65% control (average) GcR15a 57% average. At only strains show control. strain shows highest (38%), on average, similar tebuconazole (33%), then (30%). vivo, observed two isolates, while proves be more resistant The ability low temperatures promising for effective field, infections occur primarily winter.

Language: Английский

Citations

3
Fungal Grapevine Trunk Diseases in Romanian Vineyards in the Context of the International Situation DOI Creative Commons
Maria-Doinița Muntean, Ana–Maria Drăgulinescu,

Liliana Lucia TOMOIAGĂ

et al.

Pathogens, Journal Year: 2022, Volume and Issue: 11(9), P. 1006 - 1006

Published: Sept. 2, 2022

Vitis vinifera, known as the common grape vine, represents one of most important fruit crops in world. Romania is a wine-producing country with rich and long tradition viticulture. In last decade, increasing reports damage caused by grapevine trunk diseases (GTDs) have raised concerns all wine producing countries. Up to now, no study was performed regarding GTDs situation Romania, an grower Europe. this study, we aim, after comprehensive presentation fungal worldwide, review scientific information related these order open national platform international framework. achieve this, consulted over 500 references from different databases cited 309 them. Our concludes that, there little amount available literature on matter. Three out six are reported well documented Romanian viticultural zones (except for zone 4). These Eutypa dieback, Phomopsis Esca disease. Of pathogens considered responsible lata, viticola Stereum hirsutum studied Romania. Management measures quite limited, they mostly include preventive stop spread removal affected grapevines.

Language: Английский

Citations

16