Metabolomic analysis of grapes and leaves from symptomatic and asymptomatic Vitis vinifera grapevines with Esca disease

Current Plant Biology, Journal Year: 2024, Volume and Issue: 40, P. 100378 - 100378

Published: Aug. 10, 2024

Esca is a grapevine trunk disease spreading in vineyards worldwide, and of rising concern since no efficient treatment available to mitigate its impact. Trunks, grapes leaves from symptomatic asymptomatic Aragonês vines were collected at harvest stage characterise plant responses associated with this fungal disease. Presence fungi the trunks was confirmed by molecular methods using ITS region. Metabolomics analysed Gas chromatography coupled electron impact ionization time-of-flight mass spectrometry (GC-EI/TOF-MS) GC an EI/quadrupole MS (GC-EI/QUAD-MS showed that both organs plants exhibited different metabolic reprogramming than those asymptomatic. Symptomatic present lesser content tricarboxylic polyhydroxy acids, adjustment may involve salicylic acid metabolism. On other hand, fruits accumulate long-chain fatty acids probably related cuticle reinforcement changes water transport caused damage, defence-related metabolites such as α-tocopherol. berries also presented alterations volatile aroma compounds C6-volatiles, acetic suggesting on subsequent wine quality. Altogether study, identified putative markers symptomatology contributed physiological understanding could help development mitigation strategies for spread.

Language: Английский

---

A systemic approach allows to identify the pedoclimatic conditions most critical in the susceptibility of a grapevine cultivar to esca/Botryosphaeria dieback

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: May 24, 2023

ABSTRACT Esca and/or Botryosphaeria dieback (esca-BD) are two of the most destructive grapevine trunk diseases in world, disease complex which remains poorly understood. As some vine cultivars show highly variable susceptibility to esca-BD, we designed a four-year experiment identify environmental factors influence expression disease. We collected epidemiological and physiological data once year for four consecutive years 19 vineyard plots located wine-growing regions Western Switzerland. compared these with climatic obtained from weather stations same over long term. also estimated soil water holding capacity each plot. Confounding were minimal because all vineyards planted 2003 cultivar plants grafted nursery genetically homogeneous grafting material. Principal component regression analyses combined epidemiological, biotic pedoclimatic identified positive correlation between retention plant mortality due esca-BD. These showed that leaf symptoms apoplexy more frequent when cold, wet periods followed - or alternate hot, dry periods, occurs frequently conditions change abruptly (cold, May by hot June) deviate significantly long-term conditions. Regression impacts less climate is warm dry, both at regional year-specific levels. Having important towards this study allows recommendations be given winegrowers studied but can used as model fungal other cultivars, even woody plants.

Language: Английский

---

Metagenomic Study of Fungal Microbial Communities in Two PDO Somontano Vineyards (Huesca, Spain): Effects of Age, Plant Genotype, and Initial Phytosanitary Status on the Priming and Selection of their Associated Microorganisms

Plants, Journal Year: 2023, Volume and Issue: 12(12), P. 2251 - 2251

Published: June 8, 2023

The study of microbial communities associated with different plants agronomic interest has allowed, in recent years, to answer a number questions related the role and influence certain microbes key aspects their autoecology, such as improving adaptability plant host abiotic or biotic stresses. In this study, we present results characterization, through both high-throughput sequencing classical microbiological methods, fungal grapevine two vineyards ages genotypes located same biogeographical unit. is configured an approximation empirical demonstration concept "microbial priming" by analyzing alpha- beta-diversity from plots subjected bioclimatic regime detect differences structure taxonomic composition populations. were compared inventories diversity obtained culture-dependent methods establish, where appropriate, correlations between communities. Metagenomic data showed differential enrichment studied, including populations pathogens. This tentatively explained due factors time exposure infection, genotype, starting phytosanitary situation. Thus, suggest that each genotype recruits presents profiles potential antagonists pathogenic species.

Language: Английский

---

Unlocking the potential of rhizobacteria in Moroccan vineyard soils: Biocontrol of grapevine trunk diseases and plant growth promotion

Biological Control, Journal Year: 2023, Volume and Issue: 186, P. 105338 - 105338

Published: Aug. 25, 2023

Language: Английский

---

Botryosphaeria Dieback (Lasiodiplodia viticola): An Imminent Emerging Threat to the Moroccan Vineyards

Plants, Journal Year: 2022, Volume and Issue: 11(16), P. 2167 - 2167

Published: Aug. 21, 2022

A decline of various grapevines (Vitis vinifera L.) in the province Doukkala Morocco was observed 2021. The causal pathogen identified as Lasiodiplodia viticola based on morphological characteristics and phylogenetic analysis internal transcribed region (ITS), β-tubulin gene (TUB) calmodulin (cmdA). Koch's postulates were confirmed by successful re-isolation L. from plants inoculated with under controlled conditions. disease shown to be prevalent Bni Hilal (71.43%), Laamria (60%), Boulaouane (40%) districts, but quasi-absent Lmechrek. To understand dominance one grapevine trunk pathogens, effects temperature (10-40 °C) pH (pH 3-pH 12) growth sporulation investigated. species able grow a range temperatures ranging 15 40°C showed higher rate at 35 °C. fungus also characterized broad optimum between 3-12. This study is first report dealing associated diseases Morocco. Additional studies are therefore required high occurrence this vineyards, which likely due climate changes. good understanding complex might help develop reliable sustainable preventive control strategy.

Language: Английский

---

Higher Virulence of Diplodia seriata Isolates on Vines of cv. Cabernet Sauvignon Associated with 10-Year-Old Wood Compared to Young Tissue

Plants, Journal Year: 2023, Volume and Issue: 12(16), P. 2984 - 2984

Published: Aug. 18, 2023

Botryosphaeria dieback (BD) occurs in young and old plants. In the field, prevalence severity of disease increase proportionally with age vineyards. Among pathogens that cause BD, Diplodia seriata is most prevalent species Chile other countries a Mediterranean climate. To date, no information available on susceptibility adult wood to infection by this pathogen since pathogenicity tests have been carried out 1- or 2-year-old shoots detached canes. Therefore, test was plants under field conditions, inoculations 1-year-old 2- 10-year-old grapevine cv. Cabernet Sauvignon. A two isolates D. seriata. The results for show significantly more aggressive than one- two-year-old tissue, where lesions were 4.3 2.3 cm average, respectively. These compared obtained from canes after activated grape berries. Also, Chilean phylogenetically those countries, major differences found between them. Our are consistent damage observed contributing knowledge epidemiology climates. future, effect Sauvignon virulence at different tissue ages should be tested BD-causing agents wine varieties.

Language: Английский

---

MALDI mass spectrometry-based identification of antifungal molecules from endophytic Bacillus strains with biocontrol potential of Lasiodiplodia theobromae, a grapevine trunk pathogen in Peru

Current Research in Microbial Sciences, Journal Year: 2023, Volume and Issue: 5, P. 100201 - 100201

Published: Jan. 1, 2023

Lasiodiplodia theobromae, a grapevine trunk pathogen, is becoming significant threat to vineyards worldwide. In Peru, it responsible for Botryosphaeria dieback in many grapevine-growing areas and has spread rapidly due its high transmissibility; hence, control measures are urgent. It known that some endophytic bacteria strong inhibitors of phytopathogens because they produce wide range antimicrobial molecules. However, studies features from limited traditional confrontation methods. this study, MALDI mass spectrometry-based approach was performed identify characterize the antifungal molecules Bacillus velezensis M1 amyloliquefaciens M2 strains. Solid medium antagonism assays were confronting B. - L. theobromae lipopeptides identification. By TOF MS possible spectra fengycin, iturin surfactin protoned isoforms. Masses spectrums mycobacillin mycosubtilin also identified. Using Imaging we able visualize relate fengycin (1463.9 m/z) (1529.6 interaction zone during confrontations. The presence lipopeptides-synthesis genes confirmed by PCR. Liquid proteomic analysis theobromae. Different peptide sequences corresponding proteins enzymes identified MS/MS. Oxalate decarboxylase bacisubin flagellin, reported as proteins, at 99 % identity through mapping. identification would allow early selection with features. This omics tool could lead prevention diseases other economically important crops Peru.

Language: Английский

---

Role of Rain in the Spore Dispersal of Fungal Pathogens Associated with Grapevine Trunk Diseases

Plant Disease, Journal Year: 2023, Volume and Issue: 108(4), P. 1041 - 1052

Published: Oct. 12, 2023

Grapevine trunk diseases are caused by a complex of fungi that belong to different taxa, which produce spore types and have dispersal mechanisms. It is commonly accepted rainfall plays key role in dispersal, but there conflicting information the literature on relationship between rain trapping aerobiology studies. We conducted systematic review, extracted quantitative data from published papers, used pooled for Bayesian analysis effect trapping. selected 17 papers covering 95 studies 8,778 periods, concerning total 26 fungal taxa causing Botryosphaeria dieback (BD), Esca (EC), Eutypa (ED). Results confirmed these revealed differences among fungi. Rain was good predictor ED (AUROC = 0.820) BD (0.766) not ascomycetes involved EC (0.569) only basidiomycetes, Fomitiporella viticola, studied as discharge significant). Prediction more accurate negative prognosis than positive prognosis; cutoff ≥0.2 mm provided an overall accuracy ≥0.61 correct prognoses. Spores trapped rainless periods accounted <10% spores. Our had some drawbacks, were mainly knowledge gaps limited availability; drawbacks discussed facilitate further research.

Language: Английский

---

Temperature-Dependent Growth and Spore Germination of Fungi Causing Grapevine Trunk Diseases: Quantitative Analysis of Literature Data

Plant Disease, Journal Year: 2022, Volume and Issue: 107(5), P. 1386 - 1398

Published: Nov. 11, 2022

Grapevine trunk diseases (GTDs) are serious threats in all viticultural areas of the world, and their management is always complex usually inadequate. Fragmented inconsistent information on epidemiology environmental requirements causal fungi among reasons for poor disease control. Therefore, we conducted a quantitative analysis literature data to determine effects temperature mycelial growth moisture duration spore germination. Using collected information, then developed mathematical equations describing response temperature, germination different species syndromes. We considered 27 articles (selected from total 207 found through systematic search) 116 cases; these involved 43 fungal belonging three The causing Botryosphaeria dieback (BD) esca (EC) responded similarly preferred higher temperatures than those Eutypa (ED) (with optimal 25.3, 26.5, 23.3°C, respectively). At any minimal moist period required 50% was shorter BD (3.0 h) EC (17.2 or ED (15.5 h). Mathematical were accounting temperature–moisture relationships GTD fungi, which showed concordance correlation coefficients ≥0.888; such should be useful reducing risk infection.

Language: Английский

---

First Description of Non-Enzymatic Radical-Generating Mechanisms Adopted by Fomitiporia mediterranea: An Unexplored Pathway of the White Rot Agent of the Esca Complex of Diseases

Journal of Fungi, Journal Year: 2023, Volume and Issue: 9(4), P. 498 - 498

Published: April 21, 2023

Fomitiporia mediterranea (Fmed) is the primary Basidiomycota species causing white rot in European vineyards affected by Esca complex of diseases (ECD). In last few years, an increasing number studies have highlighted importance reconsidering role Fmed ECD etiology, justifying increase research interest related to Fmed’s biomolecular pathogenetic mechanisms. context current re-evaluation binary distinction (brown vs. rot) between decay pathways induced species, our aims investigate potential for non-enzymatic mechanisms adopted Fmed, which typically described as a fungus. Our results demonstrate how, liquid culture reproducing nutrient restriction conditions often found wood, can produce low molecular weight compounds, hallmark “chelator-mediated Fenton” (CMF) reaction, originally brown fungi. CMF reactions redox cycle with ferric iron, generating hydrogen peroxide and ferrous necessary reactants leading hydroxyl radical (•OH) production. These observations led conclusion that radical-generating CMF-like mechanism may be utilized potentially together enzymatic pool, contribute degrading wood constituents; moreover, indicating significant variability strains.

Language: Английский

---