Gene silencing induced by the vector EuMY-YP∆CHS4 during the Fusarium sp. - Nicotiana benthamiana interaction DOI Open Access

Nidia Esther Hau-Yama,

Marianne Soledad Luna-Rivero,

Blondy Canto‐Canché

et al.

Revista mexicana de fitopatología(en línea)/Revista mexicana de fitopatología, Journal Year: 2023, Volume and Issue: 41(3)

Published: Aug. 29, 2023

In the literature, RNA-based interference methods have been proposed for control of pathogenic fungi in plants, such as virus-mediated silencing (VIGS). The objective this work was to evaluate functionality and efficiency VIGS EuMV-YP∆CHS4 vector, which contains a fragment Fusarium sp. chitin synthase 4 (CHS4) gene sequence, constructed from EuMV-YP vector:∆AV1. vector evaluated under two conditions Nicotiana benthamiana plants previously inoculated with inoculation after vector. results showed that both treatments EuMV-YP∆CHS4, delay leaf fall during first days infection decrease stem damage almost one centimeter, compared infected fungus. addition, reduction up 30% relative expression CHS4 observed. These suggest application vectors may be efficient strategy protect against

Language: Английский

Fungal BGCs for Production of Secondary Metabolites: Main Types, Central Roles in Strain Improvement, and Regulation According to the Piano Principle DOI Open Access
Alexander A. Zhgun

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(13), P. 11184 - 11184

Published: July 6, 2023

Filamentous fungi are one of the most important producers secondary metabolites. Some them can have a toxic effect on human body, leading to diseases. On other hand, they widely used as pharmaceutically significant drugs, such antibiotics, statins, and immunosuppressants. A single fungus species in response various signals produce 100 or more Such signaling is possible due coordinated regulation several dozen biosynthetic gene clusters (BGCs), which mosaically localized different regions fungal chromosomes. Their includes levels, from pathway-specific regulators, whose genes inside BGCs, global regulators cell (taking into account changes pH, carbon consumption, etc.) metabolism (affecting epigenetic driven by velvet family proteins, LaeA, etc.). In addition, low-molecular-weight substances mediating regulatory processes. This review devoted critical analysis available data "turning on" "off" biosynthesis metabolites filamentous fungi. To describe ongoing processes, model "piano regulation" proposed, whereby pressing certain key (signal) leads extraction sound "musical instrument cell", expressed production specific metabolite.

Language: Английский

Citations

18
Phytopathogenic fungi and oomycetes causing diseases in Theobroma cacao: chemical and genetic features DOI
Luis A. Ramírez‐Camejo, Candelario Rodríguez,

Ximena Florez‐Buitrago

et al.

Fungal Biology, Journal Year: 2025, Volume and Issue: 129(3), P. 101551 - 101551

Published: Feb. 19, 2025

Language: Английский

Citations

0
Synthetic Biology in Natural Product Biosynthesis DOI
Kaushik Seshadri,

Abner N. D. Abad,

Kyle K. Nagasawa

et al.

Chemical Reviews, Journal Year: 2025, Volume and Issue: unknown

Published: March 21, 2025

Synthetic biology has played an important role in the renaissance of natural products research during post-genomics era. The development and integration new tools have transformed workflow product discovery engineering, generating multidisciplinary interest field. In this review, we summarize recent developments biosynthesis from three different aspects. First, advances bioinformatics, experimental, analytical to identify associated with predicted biosynthetic gene clusters (BGCs) will be covered. This followed by extensive review on heterologous expression bacterial, fungal plant organisms. native host-independent paradigm identification, pathway characterization, enzyme is where synthetic most prominent role. Lastly, strategies engineer pathways for structural diversification complexity generation discussed, including assembly-line megasynthase precursor-directed modification, combinatorial biosynthesis.

Language: Английский

Citations

0
Gene expression of transcripts encoding putative secreted proteins from an oil palm fungal pathogen Ganoderma boninense DOI
Mui Sie Jee, Chai‐Ling Ho, Mohd Termizi Yusof

et al.

Physiological and Molecular Plant Pathology, Journal Year: 2025, Volume and Issue: unknown, P. 102715 - 102715

Published: April 1, 2025

Language: Английский

Citations

0
Characterization of Metabolite Landscape Distinguishes Medicinal Fungus Cordyceps sinensis and other Cordyceps by UHPLC-Q Exactive HF-X Untargeted Metabolomics DOI Creative Commons

Chuyu Tang,

Xiuzhang Li,

Tao Wang

et al.

Molecules, Journal Year: 2023, Volume and Issue: 28(23), P. 7745 - 7745

Published: Nov. 24, 2023

Cordyceps represent a valuable class of medicinal fungi with potential utilization. The overexploitation and resource scarcity sinensis (CS) have led to the emergence such as militaris (CM) cicadae (CC) substitutes. value CS is often considered superior other Cordyceps, potentially owing differences in active ingredients. This study aimed evaluate composition abundance primary secondary metabolites its substitutes by untargeted metabolomics. A total 4671 from 18 superclasses were detected. rich amino acids, lipids, organic their derivatives. We statistically analyzed found 285 differential (3'-Adenylic acid, O-Adipoylcarnitine, L-Dopachrome, etc.) between CC, CM, CM which are biomarkers. L-glutamate glycerophospholipids metabolites. KEGG enrichment analysis indicated that tyrosine metabolic pathway tryptophan metabolism most differentially expressed pathways among three Cordyceps. In contrast, was enriched higher lipid when compared may be an indispensable foundation for pharmacological functions CS. conclusion, systematic, metabolomics analyses delivered precious insights into metabolite landscapes predicted components disease therapeutics.

Language: Английский

Citations

8
Intricacies of plants' innate immune responses and their dynamic relationship with fungi: A review DOI
Anjali Tripathi, Vinay Kumar Pandey,

Abhimanyu Kumar Jha

et al.

Microbiological Research, Journal Year: 2024, Volume and Issue: 285, P. 127758 - 127758

Published: May 13, 2024

Language: Английский

Citations

2
The Chromosome-Scale Genomes of Exserohilum rostratum and Bipolaris zeicola Pathogenic Fungi Causing Rice Spikelet Rot Disease DOI Creative Commons
Ke He, Chenyu Zhao, Manman Zhang

et al.

Journal of Fungi, Journal Year: 2023, Volume and Issue: 9(2), P. 177 - 177

Published: Jan. 28, 2023

Rice spikelet rot disease occurs mainly in the late stages of rice growth. Pathogenicity and biological characteristics pathogenic fungus infestation site have been primary focus research on disease. To learn more about disease, we performed whole-genome sequencing Exserohilum rostratum Bipolaris zeicola for predicting potentially genes. The B. was only recently identified rice.We obtained 16 15 scaffolds down to chromosome level E. LWI LWII, respectively. length strain approximately 34.05 Mb, G + C content whole genome 50.56%. LWII 32.21 50.66%. After prediction annotation predicted that contain 8 13 potential genes, respectively, which may be related infection. These results improve our understanding genomes update genomic databases these two species. It benefits subsequent studies mechanisms interactions with helps develop efficient control measures against

Language: Английский

Citations

4
Whole genome sequencing and analysis of the weed pathogen Trichoderma polysporum HZ-31 DOI Creative Commons

Yushan He,

Haixia Zhu

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: July 2, 2024

Abstract In order to resolve the key genes for weed control by Trichoderma polysporum at genomic level, we extracted DNA and sequenced whole genome of T. strain HZ-31 on Illumina Hiseq platform. The raw data was cleaned up using Trimmomatic checked quality FastQC. sequencing assembled SPAdes, GeneMark used perform gene prediction assembly results. results showed that size 39,325,746 bp, with 48% GC content, number encoded 11,998. A total 148 tRNAs 45 rRNAs were predicted. 782 annotated in Carbohydrase Database, 757 Pathogen-Host Interaction 67 clusters identified. addition, 1023 predicted be signal peptide proteins. annotation functional analysis sequence polymorpha provide a basis in-depth study molecular mechanism its herbicidal action more effective utilization control.

Language: Английский

Citations

1
Uncovering the Mechanisms: The Role of Biotrophic Fungi in Activating or Suppressing Plant Defense Responses DOI Creative Commons
Michel Leiva-Mora, Yanelis Capdesuñer, Ariel Villalobos-Olivera

et al.

Journal of Fungi, Journal Year: 2024, Volume and Issue: 10(9), P. 635 - 635

Published: Sept. 5, 2024

This paper discusses the mechanisms by which fungi manipulate plant physiology and suppress defense responses producing effectors that can target various host proteins. Effector-triggered immunity effector-triggered susceptibility are pivotal elements in complex molecular dialogue underlying plant-pathogen interactions. Pathogen-produced effector molecules possess ability to mimic pathogen-associated patterns or hinder binding of pattern recognition receptors. Effectors directly nucleotide-binding domain, leucine-rich repeat receptors, downstream signaling components defense. Interactions between these receptor-like kinases plants critical this process. Biotrophic adeptly exploit networks key hormones, including salicylic acid, jasmonic abscisic ethylene, establish a compatible interaction with their hosts. Overall, highlights importance understanding interplay fungal develop effective strategies for disease management.

Language: Английский

Citations

1
RNAi-biofungicides: a quantum leap for tree fungal pathogen management DOI Creative Commons

Gothandapani Sellamuthu,

Amrita Chakraborty, Ramesh R. Vetukuri

et al.

Critical Reviews in Biotechnology, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 28

Published: Dec. 8, 2024

Fungal diseases threaten the forest ecosystem, impacting tree health, productivity, and biodiversity. Conventional approaches to combating diseases, such as biological control or fungicides, often reach limits regarding efficacy, resistance, non-target organisms, environmental impact, enforcing alternative approaches. From an ecological standpoint, RNA interference (RNAi) mediated double-stranded (dsRNA)-based strategy can effectively manage fungal pathogens. The RNAi approach explicitly targets suppresses gene expression through a conserved regulatory mechanism. Recently, it has evolved be effective tool in promoting sustainable management bio-fungicides provide efficient eco-friendly disease alternatives using species-specific targeting, minimizing off-target effects. With accessible data on outbreaks, genomic resources, delivery systems, RNAi-based biofungicides promising for managing pathogens forests. However, concerns fate of molecules their potential impact organisms require extensive investigation case-to-case basis. current review critically evaluates feasibility against by delving into methods, persistence, aspects, cost-effectiveness, community acceptance, plausible future protection products.

Language: Английский

Citations

1