Mycopathologia, Journal Year: 2024, Volume and Issue: 189(3)
Published: May 5, 2024
Language: Английский
Chemical Physics Impact, Journal Year: 2023, Volume and Issue: 7, P. 100275 - 100275
Published: July 27, 2023
With increasing incidences of antifungal resistance, biofilm formation, and its predilection for vulnerable populations, Candida albicans have been reported to cause a wide range infections, from superficial skin irritations life-threatening systemic diseases. Therefore, understanding addressing the infections associated with has become an urgent imperative in realm global healthcare. As result, this study employed DFT calculations at DFT/ωB97XD/6–311++G (2d, 2p) level optimize geometric properties two compounds: (Z)-N'-(4-(4-bromophenyl)-3-(4-fluorophenyl)thiazol-2(3H)-ylidene)-2-(thiophen-2-yl)thiazole-5-carbohydrazide (4-FBC) (Z)-N'-(4-(4-bromophenyl)-3-(2-fluorophenyl)thiazol-2(3H)-ylidene)-2-(thiophen-2-yl)thiazole-5-carbohydrazide(2-FBC). Significantly, employing spectral analysis techniques such as FT-IR NMR, compounds were characterized identified. Additionally, (4-FBC 2-FBC) exhibited comparable reactivity stability, more promising potential water than gas phase. The Molecular Electrostatic Potential (MESP) density States (DOS) shed light on electrical characteristics intermolecular interactions occurring during chemical processes. Furthermore, natural bond orbital (NBO) provided insights into second-order perturbation energies presence intense interactions, evident order their concentrated interaction thus: 4-FBC_gas (668.34 kcal/mol) > 4-FBC _water (563.98 2-FBC _gas (978.64 Kcal/mol) 2-FBC_water (696.46 Kcal/mol). pharmacokinetics indicated favorable intestinal absorption, low distribution, cytotoxicity profiles, although required further optimization due slower clearance, enzyme immunotoxicity concerns. In addition, molecular docking revealed robust binding affinities significant conventional hydrogen bonds. optimal positions (best pose) complexes determined -8.7, -8.4, -8.3 kcal/mol, -8.6, -8.5, respectively, when interacting 4YDE, 3DRA, 1EAG. These findings provide strong evidence supporting pharmacological suitability effective choices inhibiting treating albicans.
Language: Английский
Citations
25
Nature Reviews Microbiology, Journal Year: 2023, Volume and Issue: 22(1), P. 46 - 59
Published: Sept. 6, 2023
Language: Английский
Citations
23
AMB Express, Journal Year: 2024, Volume and Issue: 14(1)
Published: Feb. 20, 2024
Abstract Evidence of fungal coexistence in humans points towards adaptation to the host environment, like skin. The human commensal Malassezia has evolved, especially residing sebum-rich areas mammalian body where it can get necessary nutrition for its survival. This fungus is primarily responsible skin diseases Pityriasis versicolor (PV), characterized by hypo or hyperpigmented discoloration and erythematous macules. In this manuscript, we report a 19-year-old healthy female who presented with one-year history reddish, hypopigmented, asymptomatic lesions over chest raised lesion face. Upon clinical observation, patient displayed multiple macules papules bilateral malar area face, along hypopigmented scaly present on back. Based above findings, diagnosis PV Acne vulgaris (AV) was made. Interestingly, immunocompetent didn’t have any comorbidities. isolation scrapings post-culturing, found existence three genera same region patient’s body. We further went confirm identity particular species represent Malassezia, Rhodotorula , Candida . how predominant microbial resident fungus, coexists other members mycobiome. study an applied aspect microbiology also shows important identify organism associated infections so that appropriate therapeutics be advised avoid cases relapse.
Language: Английский
Citations
8
Mycoses, Journal Year: 2025, Volume and Issue: 68(2)
Published: Feb. 1, 2025
Although there are some studies evaluating CIE incidence and associated risk factors, none assessed mortality several months after the Candida spp. BSI episode. We aimed to assess factors for outcomes, including 1-year mortality, in patients with a public tertiary-care teaching hospital Brazil. Retrospective case-control, followed by cohort study, adult who presented BSI. Participants were eligible if they had at least one echocardiogram performed no longer than 3 days before diagnosis thereafter during respective admission. was defined presence of two major Duke criteria. studied 164 (median age: 57.6 years) median Charlson comorbidity index points. Most female (54.9%), on haemodialysis 4.6% preexisting moderate/severe heart valve disease. C. albicans (36.2%) parapsilosis complex (34.4%) most frequent species identified. detected 10 (6.1%; 95% CI: 2.4%-9.8%). In multivariable analysis, age remained as independent predictors CIE. There significant difference between groups discharge. Considering low costs hazards an echocardiogram, performing it systematically all might improve ultimately survival rates.
Language: Английский
Citations
1
The Journal of Experimental Medicine, Journal Year: 2024, Volume and Issue: 221(5)
Published: March 18, 2024
The mycobiota are a critical part of the gut microbiome, but host–fungal interactions and specific functional contributions commensal fungi to host fitness remain incompletely understood. Here, we report identification new fungal commensal, Kazachstania heterogenica var. weizmannii, isolated from murine intestines. K. weizmannii exposure prevented Candida albicans colonization significantly reduced C. burden in colonized animals. Following immunosuppression mice, competitive commensalism thereby mitigated fatal candidiasis. Metagenome analysis revealed or presence among human commensals. Our results reveal within intestinal microbiota, independent bacteria immune responses, that could bear potential therapeutic value for management albicans–mediated diseases.
Language: Английский
Citations
8
Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)
Published: Oct. 30, 2023
Hybridisation is a common event in yeasts often leading to genomic variability and adaptation. The yeast Candida orthopsilosis human-associated opportunistic pathogen belonging the parapsilosis species complex. Most C. clinical isolates are hybrids resulting from at least four independent crosses between two parental lineages, of which only one has been identified. rare presence or total absence parentals amongst hypothesised be consequence reduced pathogenicity with respect their hybrids. Here, we sequence analyse genomes environmental strains isolated warm marine ecosystems. We find that majority hybrids, phylogenetically closely related hybrid isolates. Furthermore, identify missing lineage, thus providing more complete overview evolution this species. Additionally, discover phenotypic differences as well parents under conditions relevant for pathogenesis. Our results suggest origin intrinsic pathogenic potential, pave way pre-existing adaptations rendered prone than lineages colonise infect mammalian host.
Language: Английский
Citations
16
Journal of Fungi, Journal Year: 2023, Volume and Issue: 9(8), P. 798 - 798
Published: July 28, 2023
Fungal diseases cause millions of deaths per year worldwide. Antifungal resistance has become a matter great concern in public health. In recent years rates non-
Language: Английский
Citations
14
Antimicrobial Agents and Chemotherapy, Journal Year: 2024, Volume and Issue: unknown
Published: April 16, 2024
ABSTRACT Candida parapsilosis has recently emerged as a major threat due to the worldwide emergence of fluconazole-resistant strains causing clonal outbreaks in hospitals and poses therapeutic challenge limited antifungal armamentarium. Here, we used precise genome editing using CRISPR-Cas9 gain further insights into contribution mutations ERG11 , ERG3 MRR1 TAC1 genes influence allelic dosage resistance C. . Seven most common amino acid substitutions previously reported clinical isolates (including Y132F ) were engineered two fluconazole-susceptible lineages (ATCC 22019 STZ5). Each mutant was then challenged vitro against large array antifungals, with focus on azoles. Any possible change virulence also assessed Galleria mellonella model. We successfully generated total 19 different mutants, CRISPR-Cas9. Except for R398I ( ), all remaining conferred reduced susceptibility fluconazole. However, impact fluconazole varied greatly according mutation, stronger being noted G583R acting gain-of-function mutation Cross-resistance newer azoles, non-medical but non-azole antifungals such flucytosine, occasionally noted. Posaconazole isavuconazole remained active G583R, no fitness cost associated acquisition resistance. highlight distinct contributions
Language: Английский
Citations
5
Microorganisms, Journal Year: 2024, Volume and Issue: 12(5), P. 903 - 903
Published: April 30, 2024
Candida spp. spondylodiscitis is a rare condition for which treatment options are often limited. A further obstacle the duration of therapy, should be administered up to twelve months. In view long azoles are, so far, only oral strategy that can given as home therapy. case resistance or reduced susceptibility azoles, there not enough comfortable opportunities with adequate bone penetration and limited toxicity. We report first successful use rezafungin due parapsilosis azoles. 68-year-old patient, affected by paraplegia short bowel syndrome, was diagnosed spondylodiscitis, confirmed culture on vertebral biopsy after an 18-FDG PET/CT scan. He received 200 mg weekly 26 weeks, 10 weeks previous antifungal well tolerated voriconazole plus liposomal amphotericin B. had full clinical, radiologic, biochemical response therapy rezafungin, no adverse effects. Rezafungin promising osteomyelitis, especially when line therapies ineffective, poorly tolerated, contraindicated.
Language: Английский
Citations
5
Mycoses, Journal Year: 2025, Volume and Issue: 68(1)
Published: Jan. 1, 2025
ABSTRACT Background Infections with fluconazole‐resistant Candida parapsilosis have been increasing in Israeli hospitals unclear implications for patient outcomes. Objectives To determine the frequency, mechanisms, molecular epidemiology, and outcomes of azole‐resistant C. bloodstream infections four Israel. Patients/Methods isolates were collected at central Israel during varying periods from 2005 to 2022. Antifungal susceptibility testing was done using CLSI broth microdilution. Risk factors fluconazole resistance investigated logistic regression. ERG11 gene sequencing performed on all isolates. Genetic relatedness determined multilocus microsatellite genotyping. Clinical cure, microbiological eradication, mortality rates compared between fluconazole‐susceptible resistant Results A total 192 patient‐specific analysed. Resistance voriconazole detected 80 (41%) 14 (7.2%) isolates, respectively. The Y132F substitution found 91% 1% Increasing age, intensive care hospitalisation, haemodialysis, recent exposure antibiotics risk . Distinct but related genotypes predominated each centre, indicating extensive dissemination within limited transmission among them. Fluconazole associated increased likelihood failure no significant difference clinical cure mortality. Conclusions We high , attributable nosocomial spread hospital‐specific clones bearing substitution. a higher not failure. Strategies limit are needed.
Language: Английский
Citations
0