Generation of diversity in the blue cheese moldPenicillium roquefortiand identification of pleiotropic QTL for key cheese-making phenotypes DOI Open Access
Thibault Caron, Ewen Crequer,

Mélanie Le Piver

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 22, 2024

Abstract Elucidating the genomic architecture of quantitative traits is essential for our understanding adaptation and breeding in domesticated organisms. Penicillium roqueforti mold used worldwide blue cheese maturation, contributing to flavors through proteolytic lipolytic activities. The two populations display very little genetic diversity, but are differentiated carry opposite mating types. We produced haploid F1 progenies from five crosses, using parents belonging non-cheese populations. Analyses high-quality genome assemblies parental strains revealed large translocations, having occurred via a circular intermediate. Offspring genotyping with genotype-by-sequencing (GBS) several regions segregation distortion, possibly linked degeneration lineages. found transgressions relevant making, offspring more extreme trait values than strains. identified loci (QTLs) colony color, lipolysis, proteolysis, extrolite production, including mycotoxins, not growth rates. Some appeared rich QTLs both lipid protein metabolism, other production multiple extrolites, indicating that have pleiotropic impacts. corresponded known biosynthetic gene clusters, e.g., melanin or extrolites. hybrids constitute valuable producers, new allowed identifying target important paving way strain improvement. findings further contribute mechanisms underlying rapid adaptation, revealing convergent targeting major regulators.

Language: Английский

Involvement of LaeA and Velvet Proteins in Regulating the Production of Mycotoxins and Other Fungal Secondary Metabolites DOI Creative Commons

Xuwen Hou,

Liyao Liu,

Dan Xu

et al.

Journal of Fungi, Journal Year: 2024, Volume and Issue: 10(8), P. 561 - 561

Published: Aug. 8, 2024

Fungi are rich sources of secondary metabolites agrochemical, pharmaceutical, and food importance, such as mycotoxins, antibiotics, antitumor agents. Secondary play vital roles in fungal pathogenesis, growth development, oxidative status modulation, adaptation/resistance to various environmental stresses. LaeA contains an S-adenosylmethionine binding site displays methyltransferase activity. The members velvet proteins include VeA, VelB, VelC, VelD VosA for each member with a domain. can form multimeric complexes VosA-VelB VelB-VeA-LaeA. They belong global regulators mainly impacted by light. One their most important functions is regulate gene expressions that responsible metabolite biosynthesis. aim this mini-review represent the newest cognition biosynthetic regulation mycotoxins other proteins. In cases, positively production metabolites. regulated species toxigenic fungi from genera Alternaria, Aspergillus, Botrytis, Fusarium, Magnaporthe, Monascus, Penicillium mycotoxins. We control inhibit harmful while promoting useful fungi. Furthermore, should be practical strategy activating silent clusters (BGCs) obtain previously undiscovered

Language: Английский

Citations

6
Regulation of LaeA and Velvet Proteins on the Production of Mycotoxins and Other Fungal Secondary Metabolites DOI Open Access

Xuwen Hou,

Liyao Liu,

Dan Xu

et al.

Published: July 16, 2024

Fungi are rich sources of secondary metabolites agrochemical, pharmaceutical and food importances, such as mycotoxins, antibiotics antitumor agents. Secondary play vital roles in fungal pathogenesis, growth development, oxidative status modulation, adaptation/resistance to various environmental stresses. LaeA contains an S-adenosylmethionine binding site displays methyltransferase activity. The members velvet proteins include VeA, VelB, VelC, VelD VosA, for each member with a domain. can form multimeric complexes VosA-VelB VelB-VeA-LaeA. They belong global regulators mainly impacted by light. One their most important functions is regulate gene expression that responsible metabolite biosynthesis. aim this mini-review represent the newest cognition on biosynthetic regulation mycotoxins other proteins. In cases, positively regulated production metabolites. species toxigenic fungi from genera Alternaria, Aspergillus, Botrytis, Fusarium, Magnaporthe, Monascus, Penicillium mycotoxins. We control inhibit harmful while promoting useful fungi. Furthermore, should be practical strategy activating silent clusters (BGCs) obtain previously undiscovered

Language: Английский

Citations

4
Use of Penicillium roqueforti in plant-based veined-blue cheese: a source of mycotoxin hazards? DOI Creative Commons

Oriane Gauthier,

Stéphane Bernillon,

Rayan Khireddine

et al.

Food Control, Journal Year: 2025, Volume and Issue: 171, P. 111130 - 111130

Published: Jan. 1, 2025

Language: Английский

Citations

0
Identification of quantitative trait loci (QTLs) for key cheese making phenotypes in the blue-cheese mold Penicillium roqueforti DOI Creative Commons
Thibault Caron, Ewen Crequer,

Mélanie Le Piver

et al.

PLoS Genetics, Journal Year: 2025, Volume and Issue: 21(4), P. e1011669 - e1011669

Published: April 15, 2025

Elucidating the genomic architecture of quantitative traits is essential for our understanding adaptation and breeding in domesticated organisms. Penicillium roqueforti mold used worldwide blue cheese maturation, contributing to flavors through proteolytic lipolytic activities. The two populations display very little genetic diversity, but are differentiated carry opposite mating types. We produced haploid F1 progenies from five crosses, using parents belonging non-cheese populations. Analyses high-quality genome assemblies parental strains revealed large translocations, having occurred via a circular intermediate, one with footprints Starship giant mobile elements. Offspring genotyping genotype-by-sequencing (GBS) several regions segregation distortion, possibly linked degeneration lineages. found transgressions relevant making, offspring more extreme trait values than strains. identified loci (QTLs) colony color, lipolysis, proteolysis, extrolite production, including mycotoxins, not growth rates. Some appeared rich QTLs both lipid protein metabolism, other production multiple extrolites, indicating that have pleiotropic effects. corresponded known biosynthetic gene clusters, e.g., melanin or extrolites. hybrids constitute valuable producers, new allelic combinations, allowed identifying target important paving way strain improvement. findings further contribute mechanisms underlying rapid adaptation, revealing convergent targeting major regulators.

Language: Английский

Citations

0
Expanding the Toolbox for Genetic Manipulation in Pseudogymnoascus: RNAi-Mediated Silencing and CRISPR/Cas9-Mediated Disruption of a Polyketide Synthase Gene Involved in Red Pigment Production in P. verrucosus DOI Creative Commons
Diego Palma,

Vicente Oliva,

Mariana Montanares

et al.

Journal of Fungi, Journal Year: 2024, Volume and Issue: 10(2), P. 157 - 157

Published: Feb. 16, 2024

Fungi belonging to the genus Pseudogymnoascus have garnered increasing attention in recent years. One of members genus, P. destructans, has been identified as causal agent a severe bat disease. Simultaneously, knowledge species expanded, parallel with increased availability genome sequences. Moreover, exhibits great potential producer specialized metabolites, displaying diverse array biological activities. Despite these significant advancements, genetic landscape remains largely unexplored due scarcity suitable molecular tools for manipulation. In this study, we successfully implemented RNAi-mediated gene silencing and CRISPR/Cas9-mediated disruption Pseudogymnoascus, using an Antarctic strain verrucosus model. Both methods were applied target azpA, involved red pigment biosynthesis. Silencing azpA levels 90% or higher eliminated production, resulting transformants exhibiting white phenotype. On other hand, CRISPR/Cas9 system led high percentage (73%) one-nucleotide insertion, thereby inactivating abolishing The successful application represents advancement research, opening avenues comprehensive functional investigations within underexplored fungal genus.

Language: Английский

Citations

0
Generation of diversity in the blue cheese moldPenicillium roquefortiand identification of pleiotropic QTL for key cheese-making phenotypes DOI Open Access
Thibault Caron, Ewen Crequer,

Mélanie Le Piver

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 22, 2024

Abstract Elucidating the genomic architecture of quantitative traits is essential for our understanding adaptation and breeding in domesticated organisms. Penicillium roqueforti mold used worldwide blue cheese maturation, contributing to flavors through proteolytic lipolytic activities. The two populations display very little genetic diversity, but are differentiated carry opposite mating types. We produced haploid F1 progenies from five crosses, using parents belonging non-cheese populations. Analyses high-quality genome assemblies parental strains revealed large translocations, having occurred via a circular intermediate. Offspring genotyping with genotype-by-sequencing (GBS) several regions segregation distortion, possibly linked degeneration lineages. found transgressions relevant making, offspring more extreme trait values than strains. identified loci (QTLs) colony color, lipolysis, proteolysis, extrolite production, including mycotoxins, not growth rates. Some appeared rich QTLs both lipid protein metabolism, other production multiple extrolites, indicating that have pleiotropic impacts. corresponded known biosynthetic gene clusters, e.g., melanin or extrolites. hybrids constitute valuable producers, new allowed identifying target important paving way strain improvement. findings further contribute mechanisms underlying rapid adaptation, revealing convergent targeting major regulators.

Language: Английский

Citations

0