Clinical Implications of Krüpple-like Transcription Factor KLF-14 and Certain Micro-RNA (miR-27a, miR-196a2, miR-423) Gene Variations as a Risk Factor in the Genetic Predisposition to PCOS DOI Open Access
Rashid Mir, Nizar H. Saeedi, Mohammed M. Jalal

et al.

Journal of Personalized Medicine, Journal Year: 2022, Volume and Issue: 12(4), P. 586 - 586

Published: April 6, 2022

Polycystic ovary syndrome (PCOS) is a disorder with symptomatic manifestation of an array metabolic and endocrine impairments. PCOS has relatively high prevalence rate among young women reproductive age risk factor for some severe diseases such as T2DM, insulin insensitivity, obesity, while the most dominant malfunction excess testosterone showing hyperandrogenism hirsutism. MicroRNAs have been implicated mediators including obesity resistance, these can regulate multiple cellular pathways signaling adipogenesis. Genome-wide association studies during last few years also linked Krüpple-like family transcription factors KLF14, which contribute in mechanisms mammalian gene regulation, certain altered traits atherosclerosis type-2 DM. This study characterized biochemical parameters patients comprehensive serum profiling comparison to healthy controls further examined influence allelic variations miRNAs 27a (rs895819 A > G), 196a2 (rs11614913 C T), 423 (rs6505162C A), KLF14 (rs972283 G) polymorphism on susceptibility PCOS. The experimental protocol included amplification refractory mutation-specific (ARMS)-PCR detect determine presence polymorphic variants subjects. results this case−control showed that biomarkers, both endocrine, were analyzed demonstrated statistically significant alterations patients, lipids (LDL, HDL, cholesterol), T2DM markers (fasting glucose, free insulin, HOMA-IR), hormones (FSH, LH, testosterone, progesterone). distribution Krüppel-like 14 rs972283 G A, miR-27a rs895819 G, miR-196a-2 rs11614913 T genotypes within considered population was (p < 0.05), except miR-423 rs6505162 0.05). found codominant model, KLF14-AA strongly associated greater (OR 2.35, 95% CI = 1.128 4.893, p 0.022), miR-27a-GA enhanced 2.06, 1.165 3.650, 0.012), miR-196a-CT higher 1.191 3.58, 0.009). Moreover, allele KLF-14 miR-196a2 population.

Language: Английский

Identification of Interactive Genetic Loci Linked to Insulin Resistance in Metabolic Syndrome—An Update DOI Creative Commons
Imadeldin Elfaki, Rashid Mir, Mohamed E. Elnageeb

et al.

Medicina, Journal Year: 2025, Volume and Issue: 61(1), P. 83 - 83

Published: Jan. 7, 2025

Metabolic syndrome is a metabolic disorder characterized by hypertension, dyslipidemia, impaired glucose tolerance, and abdominal obesity. Impaired insulin action or resistance initiates syndrome. The prevalence of increasing all over the world. Insulin results in defective metabolism carbohydrates lipids, addition to low-grade chronic inflammation. associated with syndrome, which risk factor for number pathological conditions, such as Type 2 diabetes (T2D), cardiovascular disease (CVD), nonalcoholic fatty liver (NAFLD), polycystic ovarian (PCOS). Genome-wide association studies have increased our understanding many loci linked these diseases others. In this review, we discuss its contribution diseases. We also genetic them. Genetic testing invaluable identification stratification susceptible populations and/or individuals. After individuals been identified via screening, lifestyle modifications regular exercise, weight loss, healthy diet, smoking cessation can reduce prevent pathologies.

Language: Английский

Citations

1
Role of Selected miRNAs as Diagnostic and Prognostic Biomarkers in Cardiovascular Diseases, Including Coronary Artery Disease, Myocardial Infarction and Atherosclerosis DOI Creative Commons
Rashid Mir, Imadeldin Elfaki,

Naina Khullar

et al.

Journal of Cardiovascular Development and Disease, Journal Year: 2021, Volume and Issue: 8(2), P. 22 - 22

Published: Feb. 19, 2021

Cardiovascular diseases are the leading cause of death worldwide in different cohorts. It is well known that miRNAs have a crucial role regulating development cardiovascular physiology, thus impacting pathophysiology heart diseases. MiRNAs also been reported to be associated with cardiac reactions, myocardial infarction (MCI) and ultimately failure (HF). To prevent these diseases, proper timely diagnosis dysfunction pivotal. Though there many symptoms an irregular condition though some biomarkers available may indicate disease, authentic, specific sensitive markers need hour. In recent times, proven promising candidates this regard. They potent as they can easily detected body fluids (blood, urine, etc.) due their remarkable stability presence apoptotic bodies exosomes. Existing studies suggest valuable biomarkers. A single biomarker insufficient diagnose coronary artery disease (CAD) or acute (AMI); thus, combination prove fruitful. Therefore, review aims highlight circulating miRNA diagnostic prognostic such (CAD), (MI) atherosclerosis.

Language: Английский

Citations

46
MicroRNA and diabetic retinopathy—biomarkers and novel therapeutics DOI Open Access
Zeljka Smit‐McBride, Lawrence S. Morse

Annals of Translational Medicine, Journal Year: 2021, Volume and Issue: 9(15), P. 1280 - 1280

Published: June 15, 2021

Diabetic retinopathy (DR) accounts for ~80% of legal blindness in persons aged 20-74 years and is associated with enormous social health burdens. Current therapies are invasive, non-curative, in-effective 15-25% DR patients. This review outlines the potential utility microRNAs (miRNAs) as biomarkers therapy diabetic retinopathy. miRNAs small noncoding forms RNA that may play a role pathogenesis by altering level expression genes via single nucleotide polymorphism regulatory loops. A majority intracellular specific have been cellular changes DR. Some extracellular called circulatory microRNAs. Circulatory found to be differentially expressed serum bodily fluid patients diabetes mellitus (DM) without severity DR, future studies reveal whether could serve novel reliable detect or predict progression. Therapeutic strategies can developed utilizing natural miRNA/long (lncRNA) lncRNAs two major families non-protein-coding transcripts. They molecules fundamental processes variety mechanisms, their function tightly regulated. The recent evidence indicates cross-talk between lncRNAs. Therefore, dysregulation critical human disease pathogenesis, such long-distance communicators reprogramming agents, they embody an entirely paradigm tissue signaling interaction. By targeting miRNAs, whole pathways implicated potentially altered. Understanding endogenous roles lead diagnostic therapeutic approaches managing this frequently blinding retinal condition.

Language: Английский

Citations

44
MDMF: Predicting miRNA–Disease Association Based on Matrix Factorization with Disease Similarity Constraint DOI Open Access
Jihwan Ha

Journal of Personalized Medicine, Journal Year: 2022, Volume and Issue: 12(6), P. 885 - 885

Published: May 27, 2022

MicroRNAs (miRNAs) have drawn enormous attention owing to their significant roles in various biological processes, as well the pathogenesis of human diseases. Therefore, predicting miRNA-disease associations is a pivotal task for early diagnosis and better understanding disease pathogenesis. To date, numerous computational frameworks been proposed identify potential without escalating costs time required clinical experiments. In this regard, I propose novel framework (MDMF) identifying using matrix factorization with similarity constraint. evaluate performance MDMF, calculated area under ROC curve (AUCs) global local leave-one-out cross-validation (LOOCV). conclusion, MDMF achieved reliable AUC values 0.9147 0.8905 LOOCV, respectively, which was improvement upon previous methods. Additionally, case studies were conducted on two major cancers (breast cancer lung cancer) validate effectiveness MDMF. Comprehensive experimental results demonstrate that not only discovers efficiently but also deciphers underlying miRNAs diseases at system level.

Language: Английский

Citations

36
Expression of miR-1-3p, miR-16-5p and miR-122-5p as Possible Risk Factors of Secondary Cardiovascular Events DOI Creative Commons
Rafał Badacz, Paweł Kleczyński, Jacek Legutko

et al.

Biomedicines, Journal Year: 2021, Volume and Issue: 9(8), P. 1055 - 1055

Published: Aug. 20, 2021

Ischemic event in one arterial territory increases the risk of a subsequent ischemic event. Circulating microRNAs (miRs) emerge as potential clinical tool to assess atherothrombotic events such cardiovascular death (CVD), myocardial infarction (MI) and stroke (IS). In this prospective study, we searched for athero-specific miRs related patients with symptomatic coronary, carotid lesion, or both territories involvements. The choice particular was based on database research (Pub-Med, Bethesda, MD, USA) taking into consideration relationship development atherosclerosis prognostic value. Levels circulating (miR-1-3p, miR-16-5p, miR-34a-5p, mir-122-5p, miR-124-3p, miR-133a-3p, miR-133b, miR-134-5p, miR-208b-3p, miR-375 miR-499-5p) were compared 142 an acute resulting from and/or coronary artery stenosis, who underwent revascularization lesion. A 6-year evaluation CVD/MI/IS performed. Patients two-territory single-territory involvement differed levels miR-1-3p (p = 0.016), miR-16-5p < 0.001), miR-34a-5p 0.018), miR-122-5p 0.007), miR-124-3p 0.001) miR-499-5p 0.001). During follow-up, 62 (43.7%) episodes occurred. multivariate Cox analysis, (HR 1.0006, 95%CI 1.0001–1.0011) peripheral disease (PAD) 2.16, 1.26–3.70) associated risk; 2.73, 1.22–6.12) PAD 3.47, 1.88–6.41) CVD; 1.0001, 1.000–1.0002) creatinine level 1.02, 1.01–1.04) IS, 1.0004, 1.0001–1.0008) MI. Expression miR-1-3p, during incident ischemia may be possible factors secondary event(s).

Language: Английский

Citations

35
SNPs in miRNAs and Target Sequences: Role in Cancer and Diabetes DOI Creative Commons

Yogita Chhichholiya,

Aman Kumar Suryan,

Prabhat Suman

et al.

Frontiers in Genetics, Journal Year: 2021, Volume and Issue: 12

Published: Dec. 1, 2021

miRNAs are fascinating molecular players for gene regulation as individual miRNA can control multiple targets and a single target be regulated by miRNAs. Loss of expression is often reported to implicated in various human diseases like diabetes cancer. Recently, geneticists across the world started reporting nucleotide polymorphism (SNPs) seed sequences Similarly, SNPs also these Both scenarios lead dysregulated which may result progression diseases. In present paper, we explore their cancers well diabetes. evidence mutations other

Language: Английский

Citations

33
rs41291957 controls miR‐143 and miR‐145 expression and impacts coronary artery disease risk DOI
Ignacio Fernando Hall, Montserrat Climent,

Chiara Viviani Anselmi

et al.

EMBO Molecular Medicine, Journal Year: 2021, Volume and Issue: 13(10)

Published: Sept. 22, 2021

Language: Английский

Citations

23
Circulating miRNA as potential biomarkers for diabetes mellitus type 2: should we focus on searching for sex differences? DOI Creative Commons
Weronika Kraczkowska, Lucyna Stachowiak, Andrzej Pławski

et al.

Journal of Applied Genetics, Journal Year: 2022, Volume and Issue: 63(2), P. 293 - 303

Published: Jan. 5, 2022

microRNAs are non-coding molecules, approximately 22 nucleotides in length, that regulate various cellular processes. A growing body of evidence has suggested their dysregulated expression is involved the pathogenesis diverse diseases, including diabetes mellitus type 2 (DM2). Early onset this chronic and complex metabolic disorder frequently undiagnosed, leading to development severe diabetic complications. Notably, DM2 prevalence rising globally an increasing number articles demonstrate susceptibility, development, progression differ between males females. Therefore, paper discusses role as a source novel diagnostic biomarkers for aims underline importance sex disparity research. Taking into account urgent need sex-specific strategies DM2, recent results have shown circulating miRNAs promising candidates sex-biased biomarkers.

Language: Английский

Citations

14
Study the association of microRNA polymorphisms (miR‐146a, miR‐4513) with the risk of coronary heart diseases in Egyptian population DOI

Heba Mohamed Ali,

Doha El‐Sayed Ellakwa, Nesma M. Elaraby

et al.

Journal of Biochemical and Molecular Toxicology, Journal Year: 2022, Volume and Issue: 37(3)

Published: Dec. 21, 2022

Coronary heart disease (CHD) is the most prevalent cause of cardiovascular mortality in world. It well established that microRNAs (miRNAs) and their variants have an essential role regulating development physiology, thus impacting pathophysiology diseases. This study was designed to determine possible association miRNA polymorphisms (miRNA-146a rs2910164C/G miR-4513 rs2168518G/A) with susceptibility CHD Egyptian patients correlation different biochemical parameters. The comprised 300 participants, including 200 unrelated 100 healthy controls. Anthropometric blood parameters were measured as genetic analysis for rs2168518G/A performed all subjects using TaqMan real-time PCR assay. Our results revealed biomedical a significant between controls p < 0.05. Analyses genotype distribution (rs2910164 rs2168518) [odd ratio = 4.54, confidence interval (CI 95%) (2.41-8.53)] 0.88, (0.83-0.92)], respectively. Furthermore, statistically difference detected lipid profile levels both rs2910164 rs2168518 polymorphisms. present study's findings indicated selected polymorphisms, miR-146a could represent useful biomarker population. These characteristics personal habits environmental factors may contribute CHD.

Language: Английский

Citations

13
Changes in miRNA expression with two weight-loss dietary strategies in a population with metabolic syndrome DOI

P. Sofia Marsetti,

Fermı́n I. Milagro, M. Ángeles Zulet

et al.

Nutrition, Journal Year: 2020, Volume and Issue: 83, P. 111085 - 111085

Published: Nov. 21, 2020

Language: Английский

Citations

20