The Science of The Total Environment,
Journal Year:
2023,
Volume and Issue:
886, P. 163997 - 163997
Published: May 9, 2023
Here
we
report
the
detection
of
carbapenemase-producing
Enterobacterales
(CPE)
isolated
from
Swedish
wastewater
and
gull
faeces.
CPE
have
not
been
detected
in
samples
animals
Sweden
preceding
this
report.
Sampling
treatment
plant
(WWTP)
inlet
outlet,
sedimentation
basins,
surface
seawater
key
aquatic
bird
habitats
freshly
deposited
faeces
was
done
on
six
separate
occasions
during
May
to
September
2021.
Following
broth
enrichment,
selective
screening
putative
performed
mSuperCarba™
(CHROMagar).
Species
identification
with
MALDI-TOF.
Antimicrobial
susceptibility
testing
according
EUCAST.
In
total,
seventeen
were
verified
by
genome
sequencing
carrying
blaGES-5,
blaIMI-3,
blaOXA-181
or
blaOXA-244.
The
blaGES-5
carried
IncP
plasmids
four
different
species;
Escherichia
coli
ST10
WWTP
Raoultella
ornithinolytica
inlet,
outlet
basins
as
well
collected
at
Klebsiella
spp.
isolates
outlet.
genetic
environment
surrounding
similar
two
Citrobacter
freundii
causing
human
infections.
blaIMI-3
IncFII(Yp)
Enterobacter
ludwigii,
a
recreational
city
park
2
km
WWTP.
located
COLKP3
plasmid
found
an
E.
coli,
while
blaOXA-244
chromosomally
ST10,
both
inlet.
Phylogenetic
analysis
R.
ludwigii
indicate
that
gulls
strains
related
those
identified
samples.
results
thus
add
increasing
evidence
WWTPs
anthropogenic
reservoirs
for
mobile
elements
antibiotic-resistance
functionality.
Such
environments
could
profoundly
impact
dissemination
spread
such
via
example
birds,
thereby
warranting
further
study
surveillance.
Antimicrobial Agents and Chemotherapy,
Journal Year:
2024,
Volume and Issue:
68(2)
Published: Jan. 9, 2024
sequence
type
ST410
is
an
emerging
carbapenemase-producing
multidrug-resistant
(MDR)
high-risk
One-Health
clone
with
the
potential
to
significantly
increase
carbapenem
resistance
among
Eurosurveillance,
Journal Year:
2024,
Volume and Issue:
29(8)
Published: Feb. 22, 2024
The
dissemination
of
carbapenemase-producing
Escherichia
coli,
although
still
at
low
level,
should
be
continuously
monitored.
OXA-244
is
emerging
in
Europe,
mainly
E.
coli
.
In
Italy,
this
carbapenemase
was
reported
from
an
environmental
river
sample
2019.
We
report
clinical
isolates
OXA-244-producing
ST131
four
patients
admitted
to
acute
care
hospital
Pavia,
Italy.
association
difficult-to-detect
determinant
with
a
globally
circulating
high-risk
clone,
relevance.
Antibiotics,
Journal Year:
2024,
Volume and Issue:
13(5), P. 394 - 394
Published: April 25, 2024
Various
genetic
elements,
including
integrons,
are
known
to
contribute
the
development
of
antimicrobial
resistance.
Class
1
integrons
have
been
identified
in
E.
coli
isolates
and
associated
with
multidrug
resistance
countries
Andean
Community.
However,
detailed
information
on
gene
cassettes
located
variable
regions
is
lacking.
Here,
we
investigated
presence
diversity
class
using
an
silico
approach,
2533
whole-genome
sequences
obtained
from
EnteroBase.
IntFinder
v1.0
revealed
that
almost
one-third
contained
these
platforms.
Integron-bearing
were
environmental,
food,
human,
animal
origins
reported
all
under
scrutiny.
Moreover,
they
clones
for
their
pathogenicity
or
Integrons
carried
aminoglycoside
(aadA),
trimethoprim
(dfrA),
cephalosporin
(blaOXA;
blaDHA),
fluoroquinolone
(aac(6')-Ib-cr;
qnrB)
These
platforms
showed
higher
larger
numbers
than
previously
reported.
carrying
more
three
determined.
Monitoring
prevalence
elements
necessary
recognizing
emergent
patterns
pathogenic
bacteria,
especially
where
various
factors
recognized
favor
selection
resistant
microorganisms.
Antibiotics,
Journal Year:
2025,
Volume and Issue:
14(1), P. 83 - 83
Published: Jan. 14, 2025
Background/Objectives:
In
2022,
an
outbreak
of
H5N1
highly
pathogenic
avian
influenza
(HPAI)
killed
60%
the
largest
breeding
colony
Dalmatian
pelicans
(DPs)
in
world
at
Mikri
Prespa
Lake
(Greece),
prompting
a
multidisciplinary
study
on
HPAI
and
other
pathogens.
This
determines
antimicrobial
resistance
rates
cloacal
enterococci
Escherichia
coli
DPs.
Methods:
Fifty-two
blood
swab
samples
were
collected
from
31
nestlings
(20
DP/11
great
white
pelicans)
hatched
after
21
subadult/adult
DPs
captured
spring
migration
stopover.
The
swabs
inoculated
non-selective
chromogenic-selective
media.
Identification
was
performed
using
MALDI-TOF,
susceptibility
tested.
genetic
content
characterized
PCR
sequencing,
clonality
extended-spectrum
beta-lactamase
(ESBL)-producing
isolates
Multilocus
Sequence
Typing.
Results:
Twenty-eight
non-repetitive
E.
45
recovered
media;
most
them
susceptible
to
all
antibiotics
tested
(85.7%
coli/91.1%
enterococci).
Three
fifty-two
(6%,
adults)
contained
ESBL-E.
(detected
chromogenic
ESBL
plates),
carrying
blaCTX-M-15
gene
belonging
lineage
ST69.
Conclusions:
Despite
fecal
tested,
finding
that
ST69
carry
is
concern.
high-risk
clone
needs
further
investigation
elucidate
its
primary
sources
address
growing
threat
integrated
“One
Health”
perspective.
Furthermore,
it
imperative
potential
impacts
endangered
DP
further.
