The Science of The Total Environment,
Journal Year:
2024,
Volume and Issue:
950, P. 175344 - 175344
Published: Aug. 6, 2024
The
widespread
dissemination
of
carbapenem-resistant
bacteria
in
wastewater
systems,
particularly
from
clinical
sources,
poses
a
significant
public
health
risk.
This
study
assessed
the
concentrations
and
distributions
extracellular
DNA
(exDNA)
intracellular
(iDNA)
harboring
carbapenemase
genes
six
tertiary
care
hospitals
Germany.
We
collected
total
36
samples,
comprising
biological
replicates
each
hospital,
analysed
them
using
quantitative
real-time
PCR
(qPCR)
digital
(dPCR).
analysis
targeted
seven
genes:
bla
Environment International,
Journal Year:
2024,
Volume and Issue:
185, P. 108554 - 108554
Published: March 1, 2024
Among
the
most
problematic
bacteria
with
clinical
relevance
are
carbapenem-resistant
Enterobacterales
(CRE),
as
there
very
limited
options
for
their
treatment.
Treated
wastewater
can
be
a
route
release
of
these
into
environment
and
population.
The
aim
this
study
was
to
isolate
CRE
from
treated
Zagreb
treatment
plant
determine
phenotypic
genomic
characteristics.
A
total
200
suspected
were
isolated,
148
which
confirmed
by
MALDI-TOF
MS.
predominant
species
Klebsiella
spp.
(n
=
47),
followed
Citrobacter
40)
Enterobacter
cloacae
complex
(cplx.)
35).
All
isolates
carbapenemase
producers
multidrug-resistant
phenotype.
Using
multi-locus
sequence
typing
whole-genome
sequencing
(WGS),
18
different
types
identified
among
isolates,
14
associated
human-associated
clones.
virulence
gene
analysis
sequenced
7)
revealed
potential
pathogenicity.
PCR
WGS
showed
that
frequent
genes
in
K.
pneumoniae
blaOXA-48
blaNDM-1,
frequently
occurred
together,
while
blaKPC-2
together
blaNDM-1
mainly
detected
oxytoca,
E.
cplx.
Colistin
resistance
observed
40
%
57
isolates.
Underlying
mechanisms
include
known
potentially
novel
intrinsic
(point
mutations
pmrA/B,
phoP/Q,
mgrB
crrB
genes)
acquired
(mcr-4.3
gene).
mcr-4.3
first
time
is
probably
located
on
conjugative
IncHI1B
plasmid.
In
addition,
13
various
other
clinically
relevant
antibiotics
well
plasmids
possibly
genes.
Our
demonstrates
important
role
municipal
plays
harboring
spreading
enterobacterial
pathogens
resistant
last-resort
antibiotics.
International Journal of Hygiene and Environmental Health,
Journal Year:
2024,
Volume and Issue:
259, P. 114379 - 114379
Published: April 15, 2024
Wastewater
analysis
can
serve
as
a
source
of
public
health
information.
In
recent
years,
wastewater-based
epidemiology
(WBE)
has
emerged
and
proven
useful
for
the
detection
infectious
diseases.
However,
insights
from
wastewater
treatment
plant
do
not
allow
small-scale
differentiation
within
sewer
system
that
is
needed
to
analyze
target
population
under
study
in
more
detail.
Small-scale
WBE
offers
several
advantages,
but
there
been
no
systematic
overview
its
application.
The
aim
this
scoping
review
provide
comprehensive
current
state
knowledge
on
diseases,
including
methodological
considerations
A
database
search
was
conducted,
considering
only
peer-reviewed
articles.
Data
analyses
included
quantitative
summary
qualitative
narrative
synthesis.
Of
2130
articles,
we
278,
most
which
were
published
since
2020.
studies
analyzed
at
building
level
(n
=
203),
especially
healthcare
110)
educational
facilities
80),
neighborhood
scale
86).
main
analytical
parameters
viruses
178),
notably
SARS-CoV-2
161),
antibiotic
resistance
(ABR)
biomarkers
99),
often
by
polymerase
chain
reaction
(PCR),
with
DNA
sequencing
techniques
being
less
common.
terms
sampling
techniques,
active
dominated.
frequent
lack
detailed
information
specification
selection
criteria
characterization
sites
identified
concern.
conclusion,
based
large
number
studies,
overarching
strategic
aspects
WBE.
An
enabling
environment
requires
inter-
transdisciplinary
sharing
across
countries.
Promoting
adoption
will
benefit
common
international
conceptualization
approach,
standardized
internationally
accepted
terminology.
particular,
development
good
practices
different
warranted.
This
includes
establishment
guidelines
local
sub-sewersheds,
transparent
reporting
ensure
comparability
results.
Water,
Journal Year:
2024,
Volume and Issue:
16(9), P. 1297 - 1297
Published: May 2, 2024
This
study
investigates
the
prevalence
of
resistance
and
virulence
genes
in
Klebsiella
isolates
from
surface
waters
Northern
Portugal,
within
broader
context
freshwater
quality
challenges
Southern
Europe.
The
aim
this
research
is
to
explain
how
dynamics,
antibiotic
resistance,
biofilm
formation
interact
waters.
Antimicrobial
susceptibility
was
examined
using
Kirby–Bauer
disk
diffusion
method
against
11
antibiotics
screening
for
Extended-Spectrum
Beta-Lactamase
(ESBL)
production
double-disk
synergy.
PCR
employed
detect
genes,
while
assessed
microplate
method.
Out
77
water
isolates,
33
(14
spp.
19
K.
pneumoniae
strains)
were
isolated.
ESBL
observed
36.8%
28.6%
High
rates
blaCTX-U
both.
papC
gene
prevalent,
signifying
potential
environmental
risks.
Biofilm
averaged
81.3%
86.9%
These
findings
underscore
intricate
interplay
between
Klebsiella’s
dynamics
quality,
with
ESBL’s
raising
concerns
about
waterborne
dissemination
public
health
implications.
work
supports
need
vigilance
Animals,
Journal Year:
2023,
Volume and Issue:
13(18), P. 2922 - 2922
Published: Sept. 14, 2023
Cryptosporidium
spp.,
Giardia
duodenalis,
Enterocytozoon
bieneusi
and
Escherichia
coli
are
important
diarrheal
pathogens
threatening
the
health
of
humans
various
animals.
Goats,
especially
pre-weaned
goat
kids,
that
carry
these
reservoirs
related
to
human
infection.
In
present
study,
PCR-based
sequencing
techniques
were
applied
characterize
G.
E.
in
202
fecal
samples
kids
for
Guanzhong
dairy
goats
from
five
locations
Shaanxi
Province.
The
positive
rates
37.6%
(76/202),
16.3%
(33/202),
55.4%
(112/202)
78.7%
(159/202)
respectively.
Co-infection
two
four
was
found
114
samples.
Significant
differences
(p
<
0.001)
spp.
duodenalis
among
age
groups.
Furthermore,
species
(C.
parvum
C.
xiaoi),
assemblages
(E
A),
nine
genotypes
(CHG3,
CHG1,
BEB6,
CHG5,
CHG2,
SX1,
CHG28,
COS-II
CD6)
pathotypes
(EPEC
EHEC)
identified.
As
Cryptosporidium,
(IIdA19G1
IIdA19G2)
(XXIIIa
XXIIIg)
subtypes
recognized
xiaoi,
A
phylogenetic
analysis
based
on
ITS
locus
indicated
all
identified
this
study
belonged
group
2.
Four
virulence
factors
(ehxA,
eae,
stx2
stx1)
EPEC
EHEC
strains.
Collectively,
explored
colonization
frequency
Province
expanded
our
understanding
genetic
composition
zoonotic
potential
goats.
