Causal Associations between Gut Microbiota and Different Types of Dyslipidemia: A Two-Sample Mendelian Randomization Study

Nutrients, Journal Year: 2023, Volume and Issue: 15(20), P. 4445 - 4445

Published: Oct. 20, 2023

The determination of a causal association between gut microbiota and range dyslipidemia remains uncertain. To clarify these associations, we employed two-sample Mendelian randomization (MR) analysis utilizing the inverse-variance weighted (IVW) method. This comprehensive investigated genetic variants that exhibited significant (p < 5 × 10-8) with 129 distinct genera their potential link to different types dyslipidemia. results indicated 22 in humans. Furthermore, findings suggested impact on regulation is dependent specific phylum, family, genus. Bacillota phylum demonstrated greatest diversity, 15 distributed among eight families. Notably, microbiota-derived from Lachnospiraceae Lactobacillaceae families exhibit statistically associations lipid levels contribute overall health 0.05). sensitivity our possess robustness > investigation provide compelling evidence substantiates human body. It noteworthy highlight influence as crucial regulator levels, should be recognized probiotics significantly this metabolic process.

Language: Английский

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Gut Microbiota and Inflammation Modulation in a Rat Model for Ulcerative Colitis after the Intraperitoneal Administration of Apigenin, Luteolin, and Xanthohumol

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(6), P. 3236 - 3236

Published: March 12, 2024

Ulcerative colitis (UC) is a chronic inflammatory disorder affecting the colon, with symptomatology influenced by factors including environmental, genomic, microbial, and immunological interactions. Gut microbiota dysbiosis, characterized bacterial population alterations, contributes to intestinal homeostasis disruption aberrant immune system activation, thereby exacerbating state. This study assesses therapeutic efficacy of intraperitoneal (IP) injected flavonoids (apigenin, luteolin, xanthohumol) in reduction parameters modulation gut murine model ulcerative colitis. Flavonoids interact modulating their composition serving as substrates for fermentation into other anti-inflammatory bioactive compounds. Our results demonstrate effectiveness luteolin xanthohumol treatment enhancing relative abundance microorganisms, attenuating pro-inflammatory species. Moreover, all three exhibit cytokine levels, strongly demonstrating utility alleviating associated physical UC symptoms. suggests that this molecule potential alternative or co-therapy conventional pharmacological interventions, potentially mitigating adverse effects. A limited impact on observed apigenin, attributed its solubility constraints via chosen administration route, resulting accumulation mesentery.

Language: Английский

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Endoscopic surgery affects the gut microbiota and its metabolism in breast cancer patients

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 7, 2025

Despite the advantages of endoscopic surgery in reducing trauma and enhancing recovery for breast cancer patients, its impact on gut microbiota, which is crucial health estrogen metabolism, remains unclear. Further investigation necessary to fully understand this implications. Between June December 2022, fecal samples were collected from 20 patients who underwent surgery. The microbiota composition was determined using 16S rRNA sequencing, while metabolites analyzed through liquid chromatography-tandem mass spectrometry (LC-MS/MS). Bioinformatics statistical analyses employed identify significant alterations microbial taxa abundance assess intergroup differences. These included t-tests pairwise comparisons, one-way ANOVA multiple group chi-square tests categorical data analysis. Endoscopic subtly changed diversity composition. Post-surgery, there a reduction Lachnospiraceae, Monoglobaceae Firmicutes Bacteroides ratios. Shifts also observed, impacted pathways such as primary bile biosynthesis Ascorbate aldarate with PE(PGD1/18:1(9Z)) identified key differential metabolite that increased post-surgery. Azasetron, tyramine glucuronide, DL-DOPA, phthalide, acetophenazine, aciclovir, creatinine bicarbonate, 4-oxo-L-proline being associated distinct bacterial taxa. Breast undergoing experience shift their metabolic profiles. Therefore, postoperative management, particular focus adjustment patient outcomes.

Language: Английский

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The Role of Gut Microbiota Dysbiosis in Erectile Dysfunction: From Pathophysiology to Treatment Strategies

Microorganisms, Journal Year: 2025, Volume and Issue: 13(2), P. 250 - 250

Published: Jan. 23, 2025

Erectile dysfunction (ED) is a prevalent male sexual disorder characterized by the persistent inability to achieve or maintain an erection sufficient for satisfactory performance. While its etiology multifactorial, encompassing vascular, neurological, hormonal, and psychological components, emerging evidence suggests significant role gut microbiota dysbiosis in development. The influences various metabolic, inflammatory, neuropsychological processes critical erectile function. Dysbiosis can lead systemic inflammation, endothelial dysfunction, hormonal imbalances, altered neurotransmitter production, all of which are key factors ED pathogenesis. This narrative review synthesizes current research on association between alterations ED, highlighting specific bacterial taxa implicated through mechanisms involving metabolic disturbances, regulation. explores potential linking including pro-inflammatory cytokines, barrier integrity disruption, disorders, via gut–brain axis, Furthermore, offers promising avenues developing non-invasive biomarkers therapeutic interventions such as probiotics, prebiotics, dietary modifications, fecal transplantation. Future should focus longitudinal studies, mechanistic explorations, clinical trials validate these findings translate them into practice. Understanding interplay function could unveil novel diagnostic pave way innovative treatments targeting microbiota, ultimately improving men’s overall health.

Language: Английский

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Genetic background and microbiome drive susceptibility to epicutaneous sensitization and food allergy in adjuvant-free mouse model

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 29, 2025

Background The dual allergen exposure hypothesis states that sensitization to food antigens occurs through a damaged skin barrier in individuals with no previous oral tolerance certain foods. However, the resulting allergic reaction could depend on factors such as host’s genetic predisposition well and gut microbiota. Methods Specific-pathogen-free BALB/c C57BL/6 germ-free (GF) mice were epicutaneously sensitized ovalbumin (OVA) via dorsal tape-stripped challenged OVA by intragastric gavage. development of allergy (FA) symptoms, Th2 mast cell immune response differences microbiota investigated. Results mice, but not showed severe clinical signs FA (hypothermia, diarrhea) stronger serum antibody cytokine secretion spleen jejunum after OVA-treatment. increased count correlated higher MCPT-1 production histidine decarboxylase mRNA expression these mice. 16S rRNA sequencing analysis revealed lower abundance short-chain fatty acids producing bacteria microbiome OVA-treated Changes β-diversity reflect both background treatment experimental Compared SPF GF developed more anaphylactic hypothermia diarrhea, although they had count, serum, arachidonate 5-lipoxygenase expression. Conclusions We show are mouse strain choice for model adjuvant-free epicutaneous disrupted following development. Our results highlight significant influence susceptibility, emphasizing complex interplay between response.

Language: Английский

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In vitro modeling of feline gut fermentation: a comprehensive analysis of fecal microbiota and metabolic activity

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: Jan. 29, 2025

The gut microbiota (GM) is a large and diverse microbial community that plays essential roles in host health. vitro fermentation model of the fecal GM serves as valuable complement to food health research both humans animals. Despite advancements standardized protocols for culturing human GM, concerning animals—particularly companion animals—remains limited. This study aims identify optimal method cat by comprehensively analyzing characteristics. We evaluated seven culture media previously used simulate microenvironment humans, dogs, cats: anaerobic medium base (AMB), Minimum (MM), Pet (PM), VI (VI), VL (VL), Yeast (JM), yeast casitone fatty acid agar (YCFA). Fresh samples were fermented these 48 h, followed 16S rRNA sequencing assess bacterial composition targeted metabolite monitoring during fermentation. results revealed substrate differentially impacts structure High levels carbon nitrogen sources can substantially increase gas production, particularly CO 2 , while also significantly enhancing production short-chain acids (SCFAs). Additionally, substrates with high carbon-to-nitrogen ratio promote more SCFAs biogenic amines, enrich Bacteroidaceae family, even when total amount lower. Comprehensive analysis metabolites reveals PM effectively simulates nutrient-deficient simulation maintains stability lower levels. Therefore, using without focusing on specific genera, represents most suitable model. finding contributes understanding conditions may provide new approach investigating pharmaceuticals related

Language: Английский

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Gut commensal Lachnospiraceae bacteria contribute to anti-colitis effects of Lactiplantibacillus plantarum exopolysaccharides

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 142815 - 142815

Published: April 1, 2025

Language: Английский

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The Role of Beneficial Microbiota in COVID-19: Insights from Key Bacterial Genera

Microorganisms, Journal Year: 2025, Volume and Issue: 13(5), P. 1029 - 1029

Published: April 29, 2025

The COVID-19 pandemic has highlighted the need for a comprehensive understanding of factors influencing disease severity and progression. Emerging research indicates that human microbiota, particularly beneficial bacteria, significantly impacts immune responses health outcomes in patients. While existing studies provide general insights into relationship between microbiota probiotics with COVID-19, they often lack detailed exploration how specific bacterial taxa might be used as adjunctive treatments. This review aims to address this gap by focusing on ten key genera discussing their roles evaluating potential prevention treatment. covers impact these microbes health, population alterations patients, interactions other viral infections. Among microbes, several exhibit distinct patterns abundance highlighting infection dynamics. In populations Akkermansia, Ruminococcus, Roseburia are consistently reduced, while those Faecalibacterium show significant decline more severe cases. Bacteroides presents varying effects depending species involved. Alterations Blautia Lachnospiraceae associated increased inflammation severity. Likewise, depletion Lachnospira Coprococcus populations, both linked anti-inflammatory effects, may exacerbate symptom Oscillospira, though less studied, is connected overall could have implications synthesizes current highlight importance maintaining healthy alleviate contribute development novel therapeutic strategies involving modulation.

Language: Английский

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Lacticaseibacillus paracasei JS-3 Isolated from “Jiangshui” Ameliorates Hyperuricemia by Regulating Gut Microbiota and iTS Metabolism

Foods, Journal Year: 2024, Volume and Issue: 13(9), P. 1371 - 1371

Published: April 29, 2024

Background: A diet high in purines can impair the function of gut microbiota and disrupt purine metabolism, which is closely associated with onset hyperuricemia. Dietary regulation intestinal health maintenance are key approaches for controlling uric acid (UA) levels. Investigating impacts fermented foods offers potential dietary interventions managing Methods: In this study, we isolated a strain potent UA-degrading capabilities from “Jiangshui”, food product Gansu, China. We performed identification assessed its probiotic potential. Hyperuricemic quails, induced by high-purine diet, were used to assess UA degradation capability JS-3 measuring levels serum feces. Additionally, pathways elucidated through analyses microbiome fecal metabolomics. Results: JS-3, identified as Lacticaseibacillus paracasei, was capable eliminating 16.11% within 72 h, rapidly proliferating producing 12 surviving gastrointestinal tract. Using hyperuricemic quail models, JS-3’s capacity. Two weeks after administration (2 × 108 cfu/d per quail), (SUA) significantly decreased normal levels, renal damage quails markedly improved. Concurrently, feces group demonstrated significant UA, achieving up 49% 24 h. 16S rRNA sequencing revealed role restoration augmenting community (Bifidobacterium, Bacteroides unclassified_f-Lachnospiraceae, norank_fynorank_o-Clostridia_UCG-014) diminishing pathogenic bacteria (Macrococus Lactococcus). Corresponding rise short-chain fatty (SCFA)-producing bacteria, increased SCFA (p < 0.05, 0.01). ameliorated metabolic disturbances influencing 26 abnormal metabolites predominantly linked purine, tryptophan, bile thereby enhancing protection. Conclusions: For first time, an active strain, “Jiangshui” treatment It modulates host–microbiome interactions, metabolome, enhances degradation, reduces SUA alleviates damage, effectively treats hyperuricemia without causing damage. summary, serve therapeutic value

Language: Английский

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Effect of a topical traditional Chinese herbal medicine on skin microbiota in mouse model of atopic dermatitis

Heliyon, Journal Year: 2024, Volume and Issue: 10(13), P. e33240 - e33240

Published: June 18, 2024

This study aims to explore the impact of herbal ointment Chushi Zhiyang Ruangao (CSZYRG) on skin's microecological environment in a mouse model atopic dermatitis (AD) and understand underlying mechanisms involved. The AD was established C57 mice using calpolitol (a hypocalcemic analog vitamin D3; MC903). Medication-free matrix ointment, CSZYRG, mometasone furoate cream (positive control group) were applied injured areas. skin lesions photographed. Skin for hematoxylin eosin (H&E) staining observe any pathological changes. Serum immunoglobulin IgE detected by enzyme-linked immunosorbent assay (ELISA). changes expression inflammation-related factors TNF-α, IL-1β, IL-6 ELISA qRT-PCR. microflora samples taken 16S rDNA sequencing analyzed flora diversity, abundance, dominant mice. It concluded that CSZYRG effectively alleviates lesions, serum IgE, levels However, did not affect microbial diversity but could exert an effect community relative abundance microflora. may play therapeutic role affecting

Language: Английский

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