Probiotic cocktails accelerate baicalin metabolism in the ileum to modulate intestinal health in broiler chickens

Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology, Journal Year: 2024, Volume and Issue: 15(1)

Published: Feb. 18, 2024

Background Baicalin and probiotic cocktails are promising feed additives with broad application prospects. While known to enhance intestinal health, the potential synergistic impact of combining baicalin on gut health broiler chickens remains largely unexplored. Therefore, this study aims investigate influence combined administration composition ileal cecal microbiota in elucidate underlying mechanisms responsible for health-promoting effects. Results A total 320 1-day-old male Arbor Acres broilers were divided into 4 groups, each 8 replicates 10 chicks per replicate. Over a period 42 d, birds fed basal diet or same supplemented 37.5 g/t (BC), 1,000 (PC), combination both BC (37.5 g/t) PC (1,000 g/t). The results demonstrated that + exhibited positive effects, enhancing morphology, immune function, barrier function. This was evidenced by increased VH/CD ratio, sIgA levels, upregulated expression occludin claudin-1 ( P < 0.05). 16S rRNA analysis indicated potentiated effects BC, particularly ileum, where significantly α-diversity microbiota, altered its β-diversity, relative abundance Flavonifractor 0.05), flavonoid-metabolizing bacterium. Furthermore, positively correlated chicken ileum crypt depth had limited effect structure, group very similar microbial PC, suggesting at distal end overshadowed those BC. Conclusions We enhancement regulation cocktails. Probiotic enhanced accelerated metabolism thereby influencing structure. elucidates interaction mechanism between plant extract within host microbiota. These findings provide compelling evidence future development additive combinations.

Language: Английский

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Effects of maternal hawthorn-leaf flavonoid supplementation on the intestinal development of offspring chicks

Poultry Science, Journal Year: 2024, Volume and Issue: 103(9), P. 103969 - 103969

Published: June 24, 2024

Metabolic disorders in maternal generation during the late egg-laying period have adverse effects on neonatal development. The study was conducted to clarify of feeding hawthorn-leaf flavonoid (HF) microbial community and intestinal development chicks. Breeder hens were fed a basic corn-soybean diet, while treatment groups supplemented with 30 or 60 mg/kg HF. offspring chicks divided into CON, LHF, HHF according treatments. Maternal HF supplementation at increased average daily gain decreased feed conversion rate (P < 0.05), but did not affect intake. treatments villus height crypt depth ratio up-regulated protein expressions PCNA, IGF-1R, PI3K p-mTOR jejunum 0.05) 1-day-old 14-day-old Additionally, mRNA expression tight junction transmembrane proteins (occludin) scaffolding (ZO-1 ZO-2) 0.05). Moreover, ZO-1 could last for 14 d Interestingly, dietary altered vertically transmitted from breeder chicks, especially relative abundance probiotics (i.e., Clostridium_sensu_stricto_1) meconium which may help early gut microbiota colonization In summary, bacterial neonates might promote through IGF-1R/AKT/mTOR signaling pathway.

Language: Английский

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Dietary probiotic based on a dual-strain B. subtilis improves immunity, intestinal health and growth performance of broiler chickens

Journal of Animal Science, Journal Year: 2024, Volume and Issue: 102

Published: Jan. 1, 2024

The study investigated the effects of dietary probiotic dual-strain Bacillus subtilis on production performance, intestinal barrier parameters, and microbiota in broiler chickens. In a randomized trial, male chickens were allocated into 3 groups, control group (basal diet), BS300 diet with 300 mg/kg B. subtilis), BS500 500 subtilis). inclusion significantly reduced feed conversion ratio by 4.55% during starting phase. Both supplementation increased jejunal villus height (by 17.89% 24.8%, respectively) decreased crypt depth 27.2% 31.9%, day 21. addition elevated gene expression occludin 35. Moreover, enhanced cytokine levels immunoglobulins both serum mucosa. Microbial analysis indicated that abundance potential probiotics (Sutterella) butyrate-producing bacteria (Lachnoclostridium, Tyzzerella, Anaerostipes, Clostridium_sensu_stricto_13, Prevotellaceae_NK3B31_group, Lachnospiraceae_UCG-010). abundances Anaerostipes Sutterella, are correlated growth performance immune function. conclusion, improved potentially through regulation immunity, function, broilers. Notably, exhibited more benefits for broilers compared to mg/kg.

Language: Английский

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Effects of supplemental Glycyrrhiza polysaccharide on growth performance and intestinal health in weaned piglets

Animal Biotechnology, Journal Year: 2024, Volume and Issue: 35(1)

Published: June 11, 2024

In this study, we investigated the effects of supplemental Glycyrrhiza polysaccharide (GCP) on growth performance and intestinal health weaned piglets. Ninety piglets at 28 days age were randomly allocated to three groups with five replicates per treatment. Piglets fed following diets for days: (1) CON (control group), basal diet; (2) G500, + 500 mg/kg GCP; (3) G1000, 1000 GCP. The results showed that supplementation GCP increased average daily gain (ADG) decreased feed-to-gain ratio (F/G) (P < 0.05). Serum diamine oxidase (DAO) D-lactic acid (DL-A) levels lower in G1000 group Dietary improved mucosal trypsin activity duodenum, jejunum ileum lipase amylase Moreover, group, ZO-1, claudin 1 occludin mucosa, whereas interleukin-1β (IL-1β) IL-6 16S rRNA gene analysis indicated dietary altered jejunal microbial community, relative abundances beneficial bacteria. conclusion, can improve performance, digestive enzyme activity, immunity, barrier function community

Language: Английский

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Effects of Sea-Buckthorn Flavonoids on Growth Performance, Serum Inflammation, Intestinal Barrier and Microbiota in LPS-Challenged Broilers

Animals, Journal Year: 2024, Volume and Issue: 14(14), P. 2073 - 2073

Published: July 15, 2024

The experiment investigated the effects of sea-buckthorn flavonoids (SF) on lipopolysaccharide (LPS)-challenged broilers. A total 288 one-day-old male broilers were randomly assigned to 4 groups, with 6 replicates 12 each. lasted for 20 days. diet included two levels SF (0 or 1000 mg/kg) and intraperitoneally injected 500 μg/kg LPS 16, 18, days, an equal amount saline. challenge decreased final body weight, average daily gain, feed intake, increased feed-to-gain ratio, elevated serum IL-1β, IL-2, TNF-α, D-LA, endotoxin levels. Moreover, it resulted in a reduction IL-10 level. impaired intestinal morphology duodenum, jejunum, ileum, down-regulated mRNA relative expression Occludin, ZO-1, MUC-2 jejunum mucosa, up-regulated TLR4, MyD88, NF-κB, abundance

Language: Английский

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Soyasaponin and vertical microbial transmission: Maternal effect on the intestinal development and health of early chicks

iMeta, Journal Year: 2025, Volume and Issue: unknown

Published: May 20, 2025

Abstract Multiple factors, including genetics, nutrition, and health, influence the vertical transmission of microbiota from mothers to their offspring. Recent studies have shown that avian can be passed next generation via eggshell egg albumen. However, it remains unclear whether these microbial communities are regulated by nutrition how they associated with host genotype. Chickens, controlled rearing conditions stable genotypes, provide a promising model for investigating microbiome in birds. This study aims determine genotype‐associated bacteria vertically transmitted between generations, maternal nutritional intervention soyasaponin modulates this transfer, thereby shaping chick intestinal development informing effective strategies. We established across various anatomical sites breeder hens, chicken embryos, chicks. Avian gut reproductive tract both found chicks at developmental stages. Supplementing hen diets interacts Bifidobacterium adolescentis produce γ‐aminobutyric acid. compound offspring through distinct mechanisms epithelial cells, inhibition LC3 caspase3‐associated autophagy apoptosis pathways, as well promotion proliferation differentiation pathways mediated LGR5 Olfm4. Our highlights cloaca, yolk sac also being instrumental transfer. The incorporation affects providing theoretical basis studying effects poultry formulating corresponding dietary

Language: Английский

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Maternal supplementation with mulberry-leaf flavonoids improves the development of skeletal muscle in the offspring of chickens

Animal nutrition, Journal Year: 2024, Volume and Issue: 18, P. 72 - 83

Published: April 17, 2024

The development of skeletal muscle is a crucial factor in determining the meat yield and economic benefits broiler production. Recent research has shown that mulberry leaves their extracts can be used to significantly improve growth performance livestock poultry. present study aims elucidate mechanisms involved regulation offspring by dietary mulberry-leaf flavonoids (MLF) supplementation from perspective maternal effect theory. A total 270 Qiling breeder hens were randomly assigned 3 treatments with different doses MLF (0, 30, 60 mg/kg) for 8 weeks before collecting fertilized eggs. chicken at 13 19 d embryonic stage, 1 28 old after hatching included this study. results showed increased breast weight body embryo chick stages (

Language: Английский

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Methionine supplementation regulates eggshell quality and uterine transcriptome in late-stage broiler breeders

Animal nutrition, Journal Year: 2024, Volume and Issue: 19, P. 56 - 69

Published: July 17, 2024

This study aimed to compare the effects of dietary methionine (Met) and 2-hydroxy-4-(methylthio)-butanoate (HMTBA) on eggshell quality broiler breeder hens elucidate mechanism Met in improving from perspectives microstructure shell gland physiological function. A total 720 WOD188 at 40 wk old were assigned 3 groups, with 8 replicates per group 30 birds replicate. Over 7 weeks, fed a basal diet or same supplemented 0.15% 0.17% HMTBA. Our findings revealed significant improvements for egg shape index, thickness, breaking strength, fracture toughness (P < 0.05), whereas HMTBA showed no > 0.05). supplementation increased calcium phosphorus levels both serum tissue enhanced Ca2+ ATPase activity Histomorphological changes included mucosal fold dimensions epithelial height also improved ultrastructure, resulting thicker effective layer broader mammillae fewer type B structures The mRNA genes regulating such as OC-116, CALB1, ITM2C, significantly upregulated Transcriptome analysis identified 248 differentially group, primarily linked non-canonical Wnt/Ca2+ signaling pathway, crucial ion transport cellular proliferation. research highlights that improves by enhancing proliferation uterine function, particularly through modulation WNT11 influencing deposition ultrastructural development.

Language: Английский

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Transgenerational effects on the gene transcriptome of chicken liver

Published: Jan. 1, 2024

Language: Английский

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Replacing Hydrolyzed Soybean Meal with Recombinant β-Glucosidase Enhances Resistance to Clostridium perfringens in Broilers Through Immune Modulation

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(21), P. 11700 - 11700

Published: Oct. 31, 2024

Aglycone soy isoflavones have notable immune-regulatory bioactivity, while glycosidic forms in soybean meal pose challenges for absorption. β-Glucosidase (EC 3.2.1.21) catalyzes the non-reducing terminal β-d-glucosidic bonds, releasing β-d-glucan and aglycones. This study evaluated impact of enzymatically hydrolyzed (ESM) using recombinant β-glucosidase from

Language: Английский

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