Complete genome sequence, metabolic profiling and functional studies reveal Ligilactobacillus salivarius LS-ARS2 is a promising biofilm-forming probiotic with significant antioxidant, antibacterial, and antibiofilm potential

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: March 20, 2025

Background Probiotics restore microbial balance and prevent gut-inflammation. Therefore, finding out novel probiotic strains is a demand. As gut-microbe, benefits of Ligilactobacillus salivarius (LS) are established. However, strain-specific detailed studies limited. Here, we illustrate attributes LS-ARS2 for its potential application as food-supplement and/or therapeutic to improve gut-health. Methods Whole genome sequencing (WGS) phylogenetic analysis confirm the strain LS . To establish properties, acid-bile tolerance, auto-aggregation, cell-surface-hydrophobicity, biofilm-formation, adhesion-assays performed. ensure safety attributes, antibiotic-susceptibility, hemolytic, DNase, trypan-blue, MTT assays done. ABTS, DPPH, superoxide, hydroxyl free radical scavenging used determine anti-oxidant potential. Antibacterial assays, including co-culture assay with pathogen pathogenic biofilm-inhibition performed explore antibacterial efficacy. characterize metabolic-profile -derived cell-free-supernatant (CFS), HRMS carried out. Consequently, WGS-analyses predict molecular associations related functional outcomes. Results We find remarkable fast-growing that shows acid bile tolerance (>60% survival rate), indicating promising gut-sustainability. High auto-aggregation capacity (>80%), robust cell-surface hydrophobicity (>85%), adhesion efficacy Caco-2 cells significant gut colonization. Fascinatingly, able form biofilm within 24 h ( p < 0.0001), rare among strains, efficient stay in gut. The ensures attributes. -WGS recognizes probiotic-specific determinants, predicts genomic stability, identifies orthologous-clusters diverse functions, metabolites bacteriocins. HRMS-studies LS-ARS2- CFS further validate presence beneficial antimicrobial immunomodulatory antioxidant properties ABTS (>60%), DPPH (>10 U/mL), superoxide (>70%), (>70%). Further, activities against Gram-positive Methicillin-resistant Staphylococcus aureus (MRSA) Gram-negative multidrug-resistant clinical enterotoxigenic Escherichia coli, Vibrio cholerae, Shigella flexneri Anti- Salmonella effect prominent 0.0001). Most interestingly, -CFS inhibits MRSA -biofilm again strains. Conclusion novel, fast-growing, biofilm-forming antioxidant, antibacterial, anti-biofilm potentials, suggesting applications combating biofilms improving vivo would facilitate their applications.

Language: Английский

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Novel native probiotics with protective effects against nickel-induced kidney injury: a strategy for heavy metal toxicity

BioMetals, Journal Year: 2025, Volume and Issue: unknown

Published: April 5, 2025

Language: Английский

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Innovative mechanisms of micro- and nanoplastic-induced brain injury: Emphasis on the microbiota-gut-brain axis

Life Sciences, Journal Year: 2024, Volume and Issue: 357, P. 123107 - 123107

Published: Oct. 5, 2024

Language: Английский

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Thinking outside the brain: Gut microbiome influence on innate immunity within neurodegenerative disease

Neurotherapeutics, Journal Year: 2024, Volume and Issue: 21(6), P. e00476 - e00476

Published: Oct. 1, 2024

The complex network of factors that contribute to neurodegeneration have hampered the discovery effective preventative measures. While much work has focused on brain-first therapeutics, it is becoming evident physiological changes outside brain are best target for early interventions. Specifically, myeloid cells, including peripheral macrophages and microglia, a sensitive population cells whose activity can directly impact neuronal health. Myeloid cell includes cytokine production, migration, debris clearance, phagocytosis. Environmental measures modulate these activities range from toxin exposure diet. However, one most influential mediators fitness gut microenvironment. Here, we review current data about role in gastrointestinal disorders, Parkinson's disease, dementia, multiple sclerosis. We then delve into microbiota modulating therapies available clinical evidence their use neurodegeneration. Modulating lifestyle environmental inflammation promising interventions systematic concerted effort examine healthy aging next frontier.

Language: Английский

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Epilactose as a Promising Butyrate-Promoter Prebiotic via Microbiota Modulation

Life, Journal Year: 2024, Volume and Issue: 14(5), P. 643 - 643

Published: May 18, 2024

Epilactose is a disaccharide composed of galactose and mannose, it currently considered an “under development” prebiotic. In this study, we described the prebiotic potential epilactose by in vitro fermentation using human fecal inocula from individuals following Mediterranean diet (DM) or Vegan (DV). The effect was also compared with lactulose raffinose, interesting correlations were established between metabolites microbiota modulation. production several (lactate, short-chain fatty acids, gases) confirmed properties epilactose. For both donors, analysis showed that significantly stimulated butyrate-producing bacteria, suggesting its could be independent donor diet. Butyrate one current golden due to benefits for gut systemic health. presence epilactose, butyrate 70- 63-fold higher DM donor, when respectively. DV increase 29- 89-fold obtained conclusion, study suggests holds functional health, especially towards modulation strains.

Language: Английский

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Mindfulness, Gut–Brain Axis, and Health-Related Quality of Life: The Paradigm of IBD Patients

Healthcare, Journal Year: 2024, Volume and Issue: 12(12), P. 1209 - 1209

Published: June 17, 2024

Health-related quality of life (HRQoL) is a comprehensive measure that evaluates an individual's well-being across physical, mental, and social dimensions. Enhancing HRQoL, particularly in individuals with chronic conditions like inflammatory bowel diseases (IBD), necessitates holistic approach. Mindfulness, scientifically supported strategy for managing anxiety, has shown promise improving both physical mental health. Its benefits may be partly explained through its effects on the gut-brain axis (GBA), bidirectional communication link between gastrointestinal system central nervous system. By exploring interplay mindfulness GBA, this study aims to uncover how these elements collectively influence HRQoL healthy those IBD, offering insights into potential therapeutic pathways. A cross-sectional investigation involved 338 adults, including 50 IBD patients, utilizing validated Greek scales Mindfulness (MAAS-15), Mediterranean Diet (14-MEDAS), (EQ-5D-5L). The questionnaire gathered demographic, anthropometric, lifestyle data. Among participants, EQ-5D-5L showed moderate correlation MAAS-15 scale (r = 0.389, p < 0.05) low 14-MEDAS 0.131, 0.05). patients exhibited significantly lower mean scores than (0.75 vs. 0.85, demonstrated robust 0.414, 0.001) patients. Elevated levels emerged as predictive factors higher (OR: 1.101, 95% CI: 1.008, 1.202, 0.05, compared mindfulness). In summary, influencing diet, exhibit positive associations HRQoL. Increased predict better emphasizing clinical trials validate findings.

Language: Английский

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Overview of the Efficacy of Using Probiotics for Neurosurgical and Potential Neurosurgical Patients

Microorganisms, Journal Year: 2024, Volume and Issue: 12(7), P. 1361 - 1361

Published: July 2, 2024

This review delves into the emerging field of gut microbiota-brain axis, emphasizing its bidirectional communication and implications for neurological health, particularly in trauma neurosurgery. While disruptions this axis can lead to dysbiosis hinder recovery, recent studies have highlighted therapeutic potential interventions like probiotics targeting axis. aims focus on efficacy probiotic supplementation support trauma, neurosurgery, or pain based current clinical trials assess complex interplays among probiotics, microbiota, central nervous system (CNS). comprehensive literature identified 10 relevant publications various neurosurgical conditions across multiple countries. These demonstrated diverse outcomes, with significant improvements observed gastrointestinal mobility, inflammatory responses, infection rates, post-traumatic brain injury spinal surgery. Probiotics also showed promise mitigating antibiotic-associated diarrhea modulating cytokines. Despite promising findings, (CNS) call cautious interpretation. Conflicting outcomes emphasize need better-designed understand strain-specific disease-specific effects accurately. In conclusion, offer a adjuvant therapy patients, traumatic injuries, post-spinal However, further well-designed randomized controlled are essential elucidate intricate relationship between microbiome-modulating CNS via

Language: Английский

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An in vitro multi-organ microphysiological system (MPS) to investigate the gut-to-brain translocation of neurotoxins

Biomicrofluidics, Journal Year: 2024, Volume and Issue: 18(5)

Published: Sept. 1, 2024

The death of dopamine-producing neurons in the substantia nigra base brain is a defining pathological feature development Parkinson's disease (PD). PD is, however, multi-systemic disease, also affecting peripheral nervous system and gastrointestinal tract (GIT) that interact via gut-brain axis (GBA). Our dual-flow GIT-brain microphysiological (MPS) was modified to investigate gut-to-brain translocation neurotoxin trigger PD, 1-methyl-4-phenylpyridinium (MPP

Language: Английский

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Medicinal Cannabis and the Intestinal Microbiome

Pharmaceuticals, Journal Year: 2024, Volume and Issue: 17(12), P. 1702 - 1702

Published: Dec. 17, 2024

Historically, the multiple uses of cannabis as a medicine, food, and for recreational purposes psychoactive drug span several centuries. The various components plant (i.e., seeds, roots, leaves flowers) have been utilized to alleviate symptoms inflammation pain (e.g., osteoarthritis, rheumatoid arthritis), mood disorders such anxiety, intestinal problems nausea, vomiting, abdominal diarrhea. It has established that microbiota progresses neurological, endocrine, immunological network effects through gut–microbiota–brain axis, serving bilateral communication pathway between central enteric nervous systems. An expanding body clinical evidence emphasizes endocannabinoid system fundamental connection in regulating immune responses. This is exemplified by its pivotal role metabolic immunity equilibrium barrier integrity. neuromodulator responds internal external environmental signals while also homeostatic effector system, participating reciprocal association with microbiota. We advance an exogenous cannabinoid–intestinal microbiota–endocannabinoid axis potentiated microbiome medicinal cannabinoids supporting mechanism action system. integrative medicine model patient care advanced may provide patients beneficial health outcomes when prescribed cannabis.

Language: Английский

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Evidence of Silencing of Intestinal Inflammatory and Immune Transcripts Following Induction of Joint Inflammation

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 17, 2024

ABSTRACT Disrupted intestinal epithelial barrier function has been proposed to be integral rheumatoid arthritis (RA) progression and pathogenesis. To further define the molecular pathways in synovial inflammation response of tissues, we have used a rat model mono-joint inflammatory arthritis, induced by intra-articular injection Complete Freund’s adjuvant (CFA). The predominant single into knee joint resulted rapid reproducible formation fibrotic myeloid-infiltrated pannus. Our aim was determine how including proximal distal ileum colon, responded changes synovium temporally coordinated manner comparing their transcriptomic landscapes using RNASeq analyses. We confirmed timeline swelling measurement, increased fluid levels bikunin (a component both acute phase protein pre-alpha-inhibitor inter-alpha-inhibitor) demonstrated self-correcting trabecular cortical bone CFA challenge. Intestine-specific responses were monitored 16S microbiome amplicon sequencing, histopathology for mucus layer integrity, immune cell immunohistochemistry. present data that shows tissue displays an allostatic region specific. primarily with secretion silencing T-cell specific pathways, whereas colon showed transient upregulation macrophages, broader suppression related metabolic pathway transcripts. Interestingly, many neuropathways activated early but then suppressed later colon. There only insignificant fecal composition or post-CFA administration. In summary, our show first time following induction minimal microbiome. results help clarify tissues stresses accompany pathogenesis diseases.

Language: Английский

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