Antibiotic Resistance and Virulence Genes in Escherichia coli Isolated from Patients in a Tertiary Care Hospital: Implications for Clinical Management and Public Health DOI Creative Commons

Ali Ahsan,

Zahra Fatima,

Alina Asif

et al.

BioScientific Review, Journal Year: 2024, Volume and Issue: 6(3), P. 106 - 121

Published: Aug. 28, 2024

Background. Diarrheal diseases, exacerbated by limited access to clean water, remain a significant global health concern. Enterobacteriaceae, particularly Escherichia coli (E. coli), are their prevalent causative agents. The emergence of antibiotic resistance poses grave public threat, with extended spectrum beta-lactamases (ESBL) and carbapenemases contributing significantly. This study aimed identify the antibiogram patterns virulence genes in E. isolates obtained from patients tertiary care hospital. Method. A cross-sectional involving 395 clinical samples hospital Lahore was conducted over period six months. isolation characterization bacterial strains were performed using culture-based, biochemical, morphological assessments. Antibiotic susceptibility testing (AST) carried out Kirby-Bauer (KB) disk diffusion method. DNA extraction molecular identification through PCR. Statistical analysis Excel SPSS. Results. Of samples, found be most (47.6%), followed Klebsiella spp. (43.3%). AST revealed high cefuroxime (85%) ciprofloxacin (80%). Molecular identified traT being (37.2%), fimH aer (28.7%). Notably, sfa, papA, hly, cnf undetected. Conclusion. results showed prevalence factors rates necessitate vigilant antimicrobial stewardship. presence specific emphasizes potential pathogenicity these isolates, underscoring importance effective infection control measures.

Language: Английский

Focused Ultrasound as Targeted Therapy for Colorectal Cancer: A Comprehensive Review DOI Creative Commons
Muhammad Awais Farooqi, Mahnoor Mahnoor, Kaylee Marie Delgado

et al.

Gastrointestinal Disorders, Journal Year: 2024, Volume and Issue: 6(2), P. 380 - 401

Published: April 13, 2024

Traditional cancer treatments have not significantly improved the survival rates for individuals with colorectal cancer. As a result, there is dire need to explore novel treatment modalities that can target cancer-specific niches, transform cold tumors into hot ones, and disrupt tumor niche. Therapeutic focused ultrasound, recognized its capacity induce thermal mechanical impacts on tissue, potentially eliminate cells elicit body’s anticancer reaction by disrupting microenvironment. This article provides an overview of recent developments in employing therapeutic ultrasound (TFUS) enhance natural defenses against cancers. It also discusses studies examining utility TFUS treating patients research indicating potential stimulate response various vitro vivo models. Furthermore, it explores effects immune system highlights safety effectiveness managing cancer, providing relief from pain, improving rates. Given indications may bolster augment therapy clinical preclinical models, has emerge as pivotal tool settings.

Language: Английский

Citations

6
Factors associated with failure to disclose complete information regarding transfusion‐transmissible infections among blood donors in Lahore, Pakistan DOI
Iqra Aroob, Muhammad Alamgir Khan, Nazish Saqlain

et al.

Vox Sanguinis, Journal Year: 2025, Volume and Issue: unknown

Published: April 23, 2025

Abstract Background and Objectives This study aims to address the gap in understanding factors associated with non‐disclosure of complete information by blood donors Lahore, Pakistan. Materials Methods was a cross‐sectional study, conducted from February April 2024. The involved interviewing transfusion‐transmitted infection (TTI)‐positive three large hospitals Lahore. A validated questionnaire used analyse these donors. Results total 25,155 were screened, whom 1048 tested positive for TTIs. Among these, 218 participated study. majority them (92%) reported being unaware their infection. those who aware but still proceeded donation, primary reasons status included lack seriousness, inadequate bank staff overcrowded interview areas. significant proportion TTI‐positive uncertain sources (47%), while others high‐risk behaviours such as interaction sex workers (34%) transfusions at unauthorized clinics (7%). Future disclosure attitudes varied, 22% preferring keep confidential. Significant correlations found between future age, marital status, education, socio‐economic number donations. Conclusion These findings emphasize need targeted interventions improve donor screening pre‐donation protocols. suggests potential use adequate counselling provision appropriate sites reduce non‐compliant behaviour ensure safe donation practices.

Language: Английский

Citations

0
Focused Ultrasound, an Emerging Tool for Atherosclerosis Treatment: A Comprehensive Review DOI Creative Commons

Cynthia Imtiaz,

Muhammad Awais Farooqi, Theophilus Bhatti

et al.

Life, Journal Year: 2023, Volume and Issue: 13(8), P. 1783 - 1783

Published: Aug. 21, 2023

Focused ultrasound (FUS) has emerged as a promising noninvasive therapeutic modality for treating atherosclerotic arterial disease. High-intensity focused (HIFU), and precise that generates high temperatures at specific target sites within tissues, shown results in reducing plaque burden improving vascular function. While low-intensity (LIFU) operates lower energy levels, promoting mild hyperthermia stimulating tissue repair processes. This review article provides an overview of the current state HIFU LIFU atherosclerosis. It focuses primarily on potential due to its higher penetration ability achieve atheroma disruption. The summarizes findings from animal models human trials, covering effects FUS wall thrombolysis carotid, coronary peripheral arteries. also highlights benefits ultrasound, including noninvasiveness, targeting, real-time monitoring capabilities, making it attractive approach treatment atherosclerosis emphasizes need further investigations optimize parameters advance clinical application managing

Language: Английский

Citations

6
Comparison Of Rapid Antigen Test With RT-PCR For COVID-19 Diagnosis: Performance And Limitation DOI Creative Commons
Ahsan Ali, Chaudhry Ahmed Shabbir, M. A. Qadeer

et al.

Russian Open Medical Journal, Journal Year: 2024, Volume and Issue: 13(2)

Published: June 28, 2024

The COVID-19 pandemic has profoundly impacted healthcare systems worldwide, necessitating the development of rapid and accurate diagnostic tools. study aimed to compare performance a Rapid Antigen Test (RAK) with reverse transcription polymerase chain reaction (RT-PCR) for diagnosis, considering its global usage, promising data, convenience using saliva samples. Methods — A cross-sectional, single-blinded was conducted in Lahore, Pakistan, 7,580 samples collected between May 2021 June 2022. Three hundred twenty were tested RAK RT-PCR logistic regression analysis look at sensitivity, specificity, accuracy whether there link results cycle threshold values. Results Overall, demonstrated 67% sensitivity 75% specificity. Likelihood ratios 2.71 (positive) 0.43 (negative). disease prevalence 70.00%. PPV NPV 86.36% 50.00%, respectively. Logistic showed significant association CT values (odds ratio=6.333). Kit varied by viral load: 100% CT≤20, 63% 21-25, 22% CT>26. Conclusion This would provide an insight work efficiency commercially used antigen based screening kit.

Language: Английский

Citations

1
Eco-friendly synthesis of bioactive silver nanoparticles from black roasted gram (Cicer arietinum) for biomedical applications DOI Creative Commons
Muhammad Awais Farooqi, Sungmin Bae,

S. Kim

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Oct. 2, 2024

Language: Английский

Citations

1
Antibiotic Resistance and Virulence Genes in Escherichia coli Isolated from Patients in a Tertiary Care Hospital: Implications for Clinical Management and Public Health DOI Creative Commons

Ali Ahsan,

Zahra Fatima,

Alina Asif

et al.

BioScientific Review, Journal Year: 2024, Volume and Issue: 6(3), P. 106 - 121

Published: Aug. 28, 2024

Background. Diarrheal diseases, exacerbated by limited access to clean water, remain a significant global health concern. Enterobacteriaceae, particularly Escherichia coli (E. coli), are their prevalent causative agents. The emergence of antibiotic resistance poses grave public threat, with extended spectrum beta-lactamases (ESBL) and carbapenemases contributing significantly. This study aimed identify the antibiogram patterns virulence genes in E. isolates obtained from patients tertiary care hospital. Method. A cross-sectional involving 395 clinical samples hospital Lahore was conducted over period six months. isolation characterization bacterial strains were performed using culture-based, biochemical, morphological assessments. Antibiotic susceptibility testing (AST) carried out Kirby-Bauer (KB) disk diffusion method. DNA extraction molecular identification through PCR. Statistical analysis Excel SPSS. Results. Of samples, found be most (47.6%), followed Klebsiella spp. (43.3%). AST revealed high cefuroxime (85%) ciprofloxacin (80%). Molecular identified traT being (37.2%), fimH aer (28.7%). Notably, sfa, papA, hly, cnf undetected. Conclusion. results showed prevalence factors rates necessitate vigilant antimicrobial stewardship. presence specific emphasizes potential pathogenicity these isolates, underscoring importance effective infection control measures.

Language: Английский

Citations

0