<i>In Vitro</i> Validation of Quercetin’s Antibacterial Potency Against Hemorrhagic Septicemia in <i>Oreochromis niloticus</i> DOI
Mahendra Kumar Savita, Vinay Dwivedi, Prachi Srivastava

et al.

Toxicology International, Journal Year: 2025, Volume and Issue: unknown, P. 171 - 179

Published: March 12, 2025

Hemorrhagic septicemia caused by Aeromonas hydrophila is a major bacterial infection threatening sustainable aquaculture, particularly in species like Nile Tilapia (Oreochromis niloticus). Building on previous computational studies that identified quercetin as potential antibacterial agent against hydrophila, this study validates quercetin’s efficacy fish cell lines examining multifaceted approach, conducted FTIR to confirm the presence of and its functional group, evaluated impact DNA gyrase supercoiling inhibition cytotoxicity assessment line using MTT assay. Our findings show 80 μg/mL safe for OnIL niloticus liver line). Whereas 50, 60, 70, μg/mL, reveal potent inhibitory effects cultured with cells. This establishes effectiveness indicates could be approach managing hemorrhagic aquaculture. Major Findings: 1) The investigates use quercetin, natural polyphenol, pathogen responsible septicemia. 2) Quercetin concentrations showed strong A. when cells while at concentration was found Oreochromis (OnIL). 3) confirmed quercetin's antimicrobial activity through ability inhibit

Language: Английский

Glycyrrhizin and its derivatives: an emerging secondary metabolite arsenal of Glycyrrhiza glabra DOI
Aadil Rasool, Tanveer Ali Dar

Medicinal Chemistry Research, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 31, 2025

Language: Английский

Citations

1
Xeno-Free 3D Bioprinted Liver Model for Hepatotoxicity Assessment DOI Open Access
Ahmed S. M. Ali, Johanna Berg,

Viola Roehrs

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(3), P. 1811 - 1811

Published: Feb. 2, 2024

Three-dimensional (3D) bioprinting is one of the most promising methodologies that are currently in development for replacement animal experiments. Bioprinting and alternative technologies rely on animal-derived materials, which compromises intent welfare results generation chimeric systems limited value. The current study therefore presents first bioprinted liver model entirely void constituents. Initially, HuH-7 cells underwent adaptation to a chemically defined medium (CDM). adapted exhibited high survival rates (85–92%) after cryopreservation freezing media, comparable those preserved standard (86–92%). Xeno-free bioink 3D yielded models with relative cell viability (97–101%), akin Matrigel-based (83–102%) 15 days culture. established xeno-free was used toxicity testing marine biotoxin, okadaic acid (OA). In 2D culture, OA virtually identical cultured under conditions CDM. model, 3-fold higher concentrations than respective monolayer culture were needed induce cytotoxicity. conclusion, this describes time its applicability research purposes.

Language: Английский

Citations

8
Predicting pharmacodynamic effects through early drug discovery with artificial intelligence-physiologically based pharmacokinetic (AI-PBPK) modelling DOI Creative Commons

Keheng Wu,

Xue Li, Zhou Zhou

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Feb. 16, 2024

Graphical Abstract Main steps used to predict PK and PD outcomes of the compounds. (Step 1) Use different AI related simulations compound’s ADME physiochemical properties. 2) Predict using PBPK model. 3) models are how changes in drug concentrations affect gastric acid secretion pH. E/E0 is relative activity H + /K ATPase by drug; k sec rate constants for intra-gastric concentration; out elimination constant obs observed concentration ; I (Inhibition) current antisecretory effect (or pH level) max maximum possible can achieve; The term (I -I) represents far from its potential.

Language: Английский

Citations

8
Insights into artificial intelligence utilisation in drug discovery DOI Open Access

Abdallah Abou Hajal,

Ahmad Z. Al Meslamani

Journal of Medical Economics, Journal Year: 2024, Volume and Issue: 27(1), P. 304 - 308

Published: Feb. 22, 2024

Citations

8
Transforming Toxicity Assessment through Microphysiology, Bioprinting, and Computational Modeling DOI Open Access

Tamer A. Addissouky

Advances in Clinical Toxicology, Journal Year: 2024, Volume and Issue: 9(1), P. 1 - 14

Published: Jan. 1, 2024

Background: Traditional toxicity testing emphasizes animal models with growing concerns regarding predictive capacity, throughput and ethics. Rapid innovation surrounding human cell platforms, bioengineered tissues, omics techniques computational tools offers more modern alternatives aligned expanding knowledge of chemical biological pathways. These disruptive approaches promise immense potential to transform next-generation safety assessment drug development pipelines. Purpose: This review provides clinical researchers an updated, comprehensive perspective across evolving areas focus in new methods analysis latest advances translational context. Main Body: We survey progress two- three-dimensional cultures recapitulating tissue/organ complexity impossible conventional assays. Complementing this, modeling integrates structure-activity relationships, physicochemical properties physiological interactions predict pharmacokinetics silico. Expanding model organisms add further dimensionality demographic relevance. High-throughput imaging technologies unravel mechanisms illuminate biomarkers undetectable by standard measures. Specialized show high addressing toxicodynamic intricacies within disease contexts like diabetes NAFLD. Evaluating traditional medicines phytochemicals likewise represents area growth well-suited for contemporary platforms. Future outlook weighs remarkable advantages reducing demands, enabling precision toxicology links medicine overhauling core risk frameworks. Conclusion: intends catalyze discourse on strategic optimization priorities roadmaps towards fully unlocking the yet still emerging public health these poising transformation sciences centered human-focused models.

Language: Английский

Citations

6
AI-powered revolution in plant sciences: advancements, applications, and challenges for sustainable agriculture and food security DOI Creative Commons

Deependra Kumar Gupta,

Anselmo Pagani, Paolo Zamboni

et al.

Published: Aug. 6, 2024

Artificial intelligence (AI) is revolutionizing plant sciences by enabling precise species identification, early disease diagnosis, crop yield prediction, and precision agriculture optimization. AI uses machine learning image recognition to aid ecological research biodiversity conservation. It plays a crucial role in breeding accelerating the development of resilient, high-yielding crops with desirable traits. models using climate soil data contribute sustainable food security. In phenotyping, automates measurement analysis characteristics, enhancing our understanding growth. Ongoing aims improve models’ robustness interpretability while addressing privacy algorithmic biases. Interdisciplinary collaboration essential fully harness AI’s potential for sustainable, food-secure future.

Language: Английский

Citations

5
How Thymoquinone from Nigella sativa Accelerates Wound Healing through Multiple Mechanisms and Targets DOI Creative Commons
Abdalsalam Kmail, Omar Said, Bashar Saad

et al.

Current Issues in Molecular Biology, Journal Year: 2023, Volume and Issue: 45(11), P. 9039 - 9059

Published: Nov. 13, 2023

Wound healing is a multifaceted process necessitating the collaboration of numerous elements to mend damaged tissue. Plant and animal-derived natural compounds have been utilized for wound treatment over centuries, with many scientific investigations examining these compounds. Those antioxidant, anti-inflammatory, antibacterial properties are particularly noteworthy, as they target various wound-healing stages expedite recovery. Thymoquinone, derived from Nigella sativa (N. sativa)—a medicinal herb long history use in traditional medicine systems such Unani, Ayurveda, Chinese, Greco-Arabic Islamic medicine—has demonstrated range therapeutic properties. Thymoquinone exhibits antimicrobial, antineoplastic activities, positioning it potential remedy skin pathologies. This review examines recent research on how thymoquinone accelerates mechanisms behind its effectiveness. We carried out comprehensive literature electronic databases, including Google Scholar, PubMed, Science Direct, MedlinePlus. Our aim was gather relevant papers published between 2015 August 2023. The main criteria inclusion were that articles had be peer reviewed, original, written English, discuss parameters repair. focused effects cellular molecular involved healing. also examined role cytokines, signal transduction cascades, clinical trials. found sufficient evidence support effectiveness promoting However, there no consensus most effective concentrations substances. It therefore essential determine optimal doses best route administration. Further needed investigate side performance

Language: Английский

Citations

12
Smart Enzyme‐Like Polyphenol‐Copper Spray for Enhanced Bacteria‐Infected Diabetic Wound Healing DOI
Nannan Shao, Siyan Huang, Yueyue Huang

et al.

Small, Journal Year: 2023, Volume and Issue: 20(22)

Published: Dec. 15, 2023

Abstract Developing functional medical materials is urgent to treat diabetic wounds with a high risk of bacterial infections, glucose levels and oxidative stress. Here, smart copper‐based nanocomposite acidic spray has been specifically designed address this challenge. This pH‐responsive multienzyme‐like properties. It enables the effectively eliminate bacteria alleviate tissue pressure, thereby accelerating healing infected wounds. The works by generating hydroxyl radicals through catalysing H 2 O , which sterilization efficiency 97.1%. As alkaline micro‐vessel leakage neutralizes spray, modifies its enzyme‐like activity radicals. reduces level reactive oxygen species in 45.3%, leading similar wound‐healing effect between M1 mice non‐diabetic ones day 8. provides responsive regulated microenvironment for treating also offers convenient novel approach challenges associated wound healing.

Language: Английский

Citations

12
Mushroom-Derived Innovations: Sustainable Biomaterials for Biomedical Engineering DOI
Shishir Srivastava,

Palak Mathur,

Preeti Prakash

et al.

Deleted Journal, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 23, 2024

This study investigates the transformative potential of mushroom-derived biomaterials within biomedical engineering, presenting them as sustainable and eco-friendly alternatives to conventional materials. By examining distinct characteristics chemical compositions various mushroom species, we highlight their suitability for creating innovative biomaterials. The paper focuses on key components, such polysaccharides, fungal mycelium, chitin/chitosan, demonstrating applications in tissue antimicrobial treatments, drug delivery systems. biodegradability cultivation mushrooms underscore environmental advantages, aligning with global sustainability goals. Through detailed case studies, illustrate successful these medical devices, construction, packaging, showcasing versatility effectiveness. also addresses current challenges proposes future research directions, emphasizing need interdisciplinary collaboration ensure safety, biocompatibility, ethical use mushroom-based Our provides a comprehensive roadmap harnessing materials, paving way significant advancements devices contributing more future. We have demonstrated that are promising frontier material science, revolutionize field contribute environment.

Language: Английский

Citations

4
Phytochemical Profiling and Therapeutic Potential of Thyme (Thymus spp.): A Medicinal Herb DOI Creative Commons
Marwa Waheed, Muhammad Bilal Hussain, Farhan Saeed

et al.

Food Science & Nutrition, Journal Year: 2024, Volume and Issue: 12(12), P. 9893 - 9912

Published: Nov. 1, 2024

ABSTRACT Thymol is a phenol monoterpene that naturally derived from cymene and an isomer of carvacrol. It constitutes significant portion (10%–64%) the essential oils found in thyme ( Thymus vulgaris L., Lamiaceae), medicinal plant renowned for its therapeutic properties. Wild native to Mediterranean region has been used cooking medicine long time. In contemporary contexts, both thymol offer diverse functional applications pharmaceutical, food, cosmetic industries. attracted scientific interest potential pharmaceuticals nutraceuticals. Studies have explored efficacy treating respiratory, nervous, cardiovascular disorders, highlighting promising role interventions. Additionally, this compound demonstrates antimicrobial, antioxidant, anticarcinogenic, anti‐inflammatory, antispasmodic also shows as growth enhancer immunomodulatory properties well. Other discussed aspects include toxicity, bioavailability, metabolism, distribution animals humans. This review summarizes most data regarding beneficial effects bioactive compounds their food preservative while taking into account extract oil.

Language: Английский

Citations

4