Synthesis of silver nanoparticles using plant extracts from Erythrina abyssinica aerial parts and assessment of their anti-bacterial and anti-oxidant activities

Results in Chemistry, Journal Year: 2022, Volume and Issue: 4, P. 100402 - 100402

Published: Jan. 1, 2022

In the present study, silver nanoparticles were synthesized using Erythrina abyssinica aerial parts as both reducing and capping agents. Three extracts obtained from plant methanol, acetone water. Successful biosynthesisof was confirmed Ultraviolet–Visible Spectrophotometry that recorded Surface Plasmonic Resonance peak at around 420 nm. Phenyl, hydroxyl carbonyl functional groups in identified Fourier Transform Infrared Spectrophotometry. Morphology of studied Scanning electron microscopy technique. The optimum conditions for synthesis found to be; a pH 13, 80 °C 5 mM nitrate. antioxidant antimicrobial activities comparable ascorbic acid. A 73.4% scavenging activity 400 μg/mL nanoparticle obtained. At 100 concentration, zones inhibition E. coli Salmonella be 17.8 ± 0.11 mm 16.9 0.10 mm, respectively.

Language: Английский

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Potential Treatment of Dermatophyte Trichophyton rubrum in Rat Model Using Topical Green Biosynthesized Silver Nanoparticles with Achillea santolina Extract

Molecules, Journal Year: 2023, Volume and Issue: 28(4), P. 1536 - 1536

Published: Feb. 5, 2023

Trichophyton rubrum is the most common dermatophyte, and can cause cutaneous infections in humans animals (dermatophytosis). In this study, we investigated anti-dermatophytic potential of green synthesized silver nanoparticles using Achillea santolina extract (AS-AgNPs) an vitro vivo rat model dermal T. dermatophytosis (TRD). The synthesis AS-AgNPs was performed A. characterized by UV-VIS spectroscopy, zeta potential, imaging (transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) Energy dispersive analysis (EDX). antifungal activity determined broth microdilution method, conidial germination, hyphal growth inhibition. TEM SEM were used to study mode action AS-AgNPs. inhibited with MIC 128 μg/mL, suppressed germination 55.3% 84.6%, respectively. caused modified mycelial structures, increased cell membrane permeability, wall damage. significantly increase permeability fungal membrane, as revealed reducing ergosterol biosynthesis. An intracellular ROS induction apoptosis also observed during AS-AgNP treatment. addition, reduced integrity, shown reduction β-(1,3)-d-glucan synthase chitin activities. showed very low toxicity on primary human fibroblasts (HDF) at MIC. topical treatment infected skin TRD a significant fugal burden after 7 days complete clearance conidia, high recovery epidermal structures 14 days, compared control rats. Interestingly, attenuated infiltrated inflammatory cells, association tissue proinflammatory cytokines including TNF-α, IL-1, IL-6, MOP IL-17. conclusion, our data prove be novel therapy for rubrum.

Language: Английский

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Experimental and theoretical investigations of the effect of bis-phenylurea-based aliphatic amine derivative as an efficient green corrosion inhibitor for carbon steel in HCl solution

Heliyon, Journal Year: 2023, Volume and Issue: 9(10), P. e20254 - e20254

Published: Sept. 22, 2023

A novel bis-phenylurea-based aliphatic amine (BPUA) was prepared via a facile synthetic route, and evaluated as potential green organic corrosion inhibitor for carbon steel in 1.0 M HCl solutions. NMR spectroscopy experiments confirmed the preparation of targeted structure. The inhibitory behavior prospective compound explored experimentally by electrochemical methods theoretically DFT-based quantum chemical calculations. Obtained results revealed an outstanding performance BPUA, with efficiency 95.1% at concentration 50 mg L−1 25 °C. has improved resistivity noticeably reduced rate from 33 to 1.7 mils per year. Furthermore, adsorption study elucidates that mechanism inhibition activity obeys Langmuir isotherm mixed physisorption/chemisorption modes BPUA derivatives on surface. Calculated Gibb's free energy process ranges −35 −37 kJ mol−1.The SEM morphology analysis validates measurements substantiates corrosion-inhibiting properties BPUA. Additionally, eco-toxicity assessment human epithelial MCF-10A cells proved environmental friendliness derivatives. Density functional theory (DFT) calculations correlated inhibitor's structure corresponding behavior. Quantum descriptors disclosed potentiality onto surface through heteroatom-based groups aromatic rings.

Language: Английский

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DIA/SWATH-Mass Spectrometry Revealing Melanoma Cell Proteome Transformations with Silver Nanoparticles: An Innovative Comparative Study

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(5), P. 2029 - 2029

Published: Feb. 26, 2025

Melanoma is an aggressive cancer with rising incidence and high mortality rates, largely due to chemotherapy resistance molecular dysregulation. Nanotechnology, particularly silver nanoparticles (AgNPs), has emerged as a promising therapeutic avenue because of the nanoparticles' ability induce oxidative stress apoptosis in cells. However, conventional colloidal AgNPs lack selectivity, often causing significant damage healthy In this study, we introduce green synthesis using plant extracts, providing eco-friendly alternative improved antitumor selectivity compared traditional AgNPs. Leveraging label-free Data-Independent Acquisition/Sequential Window Acquisition All Theoretical Mass Spectrometry (DIA/SWATH MS) quantitative proteomics, investigated effects green-synthesized versus on A375 melanoma cells at 24 48 h. Our findings reveal that selectively reduced cell viability while sparing keratinocytes (HaCaT), benefit not observed Proteomic analysis highlighted significantly downregulated oncogenes, enhanced carbohydrate metabolism, disrupted copper homeostasis This marks first study explore differential integrated proteomic approach, underscoring potential targeted sustainable option for therapy.

Language: Английский

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Caffeine-Boosted Silver Nanoparticles Target Breast Cancer Cells by Triggering Oxidative Stress, Inflammation, and Apoptotic Pathways

Journal of Pharmaceutical Sciences, Journal Year: 2025, Volume and Issue: unknown, P. 103802 - 103802

Published: April 1, 2025

Language: Английский

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Biogenic silver NPs alleviate LPS-induced neuroinflammation in a human fetal brain-derived cell line: Molecular switch to the M2 phenotype, modulation of TLR4/MyD88 and Nrf2/HO-1 signaling pathways, and molecular docking analysis

Biomaterials Advances, Journal Year: 2023, Volume and Issue: 148, P. 213363 - 213363

Published: March 3, 2023

Language: Английский

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Advanced biomaterial agent from chitosan/poloxamer 407-based thermosensitive hydrogen containing biosynthesized silver nanoparticles using Eucalyptus camaldulensis leaf extract

PLoS ONE, Journal Year: 2023, Volume and Issue: 18(10), P. e0291505 - e0291505

Published: Oct. 20, 2023

Context The emergence of multidrug-resistant (MDR) pathogens poses a significant challenge for global public health systems, increasing hospital morbidity and mortality prolonged hospitalization. Objective We evaluated the antimicrobial activity thermosensitive hydrogel containing bio-synthesized silver nanoparticles (bio-AgNPs) based on chitosan/poloxamer 407 using leaf extract Eucalyptus calmadulensis . Results was prepared by cold method after mixing ingredients left at 4°C overnight to ensure complete solubilization poloxamer 407. stability formulation room temperature 3 months, absorption peak (420 nm) NPs remained unchanged. demonstrated rapid gelation under physiological conditions, excellent water retention (85%), broad-spectrum against MDR clinical isolates ATCC strains. In this regard, minimum inhibitory concentration microbial values bio-AgNPs ranged from 2–8 μg/mL 8−128 μg/mL, respectively. Formulation concentrations <64 showed no cytotoxic effect human-derived macrophages (THP-1 cells) with induction inflammation. Conclusions formulated could be used in biomedical applications as it possesses broad spectrum anti-inflammatory properties without toxic effects human cells.

Language: Английский

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Green silver nanoparticles: Prospective nanotools against neurodegenerative cell line model

Ibrain, Journal Year: 2024, Volume and Issue: 10(2), P. 123 - 133

Published: May 23, 2024

Abstract Neurodegenerative diseases represent an increasingly burdensome challenge of the past decade, primarily driven by global aging population. Ongoing efforts focus on implementing diverse strategies to mitigate adverse effects neurodegeneration, with goal decelerating pathology progression. Notably, in recent years, it has emerged that use nanoparticles (NPs), particularly those obtained through green chemical processes, could constitute a promising therapeutic approach. Green NPs, exclusively sourced from phytochemicals, are deemed safer compared NPs synthetized conventional route. In this study, chemistry‐derived silver (AgNPs) were assessed neuroblastoma cells, SHSY‐5Y, which considered pivotal model for investigating neurodegenerative diseases. Specifically, we used two different concentrations (0.5 and 1 µM) AgNPs time points (24 48 h) evaluate impact cells observing viability reduction intracellular calcium production, especially using µM at h. Furthermore, investigation atomic force microscopy (AFM) unveiled alteration Young's modulus due reorganization cortical actin following exposure AgNPs. This evidence was further corroborated confocal acquisitions as well coherency density analyses fibers. Our vitro findings suggest potential efficacy against neurodegeneration; therefore, vivo studies imperative optimize possible protocols.

Language: Английский

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An Updated Review on Ag NP Effects at Organismal Level: Internalization, Responses, and Influencing Factors

Reviews of Environmental Contamination and Toxicology, Journal Year: 2022, Volume and Issue: 260(1)

Published: Dec. 1, 2022

Language: Английский

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Green and Sustainable Approaches in Pharmaceutical Sciences

Published: Oct. 25, 2023

Green chemistry has changed the prospects of pharmaceutical industry, which is now focused on innovative changes. The aim and objective these changes are to design safe, effective, potent drugs. Industries always welcome ideas, use safer raw materials, high yields, alternative steps, or catalysts biomaterials. This chapter focuses green technologies, benefits associated with them, white biotechnology chemistry, impact approaches in industry regarding greener solvents, nanoparticle formulations, antimicrobial bandages, synthesis will be useful for researchers, scientists, those industries who working areas technologies chemistry.

Language: Английский

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