Comparative catalytic analysis of non-biodegradable barium oxide nanoparticles and biodegradable barium oxide/sodium alginate nanocomposite against organic dyes

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 304, P. 140749 - 140749

Published: Feb. 6, 2025

Language: Английский

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Directed preparation, structure–activity relationship and applications of alginate oligosaccharides with specific structures: A systematic review

Food Research International, Journal Year: 2023, Volume and Issue: 170, P. 112990 - 112990

Published: May 18, 2023

Language: Английский

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Molecular Engineering of Alginate Lyases and the Potential Agricultural Applications of Their Enzymatic Products

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 26, 2025

Alginate lyases, enzymes that degrade alginate into unsaturated oligosaccharides, have attracted significant attention for their potential applications across various fields, particularly in agriculture. This review focuses on the molecular engineering of lyases to enhance activity, stability, and specificity as well agricultural resulting enzymatic products, known oligosaccharides (AOS). We start by summarizing sources classification followed recent advances through directed evolution, rational design, truncation noncatalytic domains, conserved domain reconstruction. then explore diverse AOS, including ability promote plant growth, increase content active components, extend fruit shelf life, resistance abiotic stresses. Furthermore, value AOS feed additives preservatives shrimp-based products is also assessed. will not only lay a solid theoretical foundation but serve catalyst innovative development practical application high-value preparations utilization AOS-related providing new solutions sustainable agriculture food industry.

Language: Английский

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Recent advances in the production, properties and applications of alginate oligosaccharides - a mini review

World Journal of Microbiology and Biotechnology, Journal Year: 2023, Volume and Issue: 39(8)

Published: May 24, 2023

Language: Английский

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Directed preparation of algal oligosaccharides with specific structures by algal polysaccharide degrading enzymes

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 277, P. 134093 - 134093

Published: July 23, 2024

Language: Английский

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A Novel Bifunctional Alginate Lyase and Antioxidant Activity of the Enzymatic Hydrolysates

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(8), P. 4116 - 4126

Published: Feb. 19, 2024

Alginate lyase Aly448, a potential new member of the polysaccharide (PL) 7 family, which was cloned and identified from macroalgae-associated bacterial metagenomic library, showed bifunctionality. The molecular docking results revealed that Aly448 has two completely different binding sites for alginate (polyMG), poly-α-l-guluronic acid (polyG), poly-β-d-mannuronic (polyM) substrates, respectively, might be basis enzyme's Truncational confirmed predicted key residues affected bifunctionality but did not wholly explain. Besides, presented excellent biochemical characteristics, such as higher thermal stability pH tolerance. Degradation polyMG, polyM, polyG substrates by produced tetrasaccharide (DP4), disaccharide (DP2), galactose (DP1), exhibited antioxidant activity. These findings provide novel insights into substrate recognition mechanism bifunctional lyases pave path exploitation natural agents.

Language: Английский

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Sustainable Production of Ulva Oligosaccharides via Enzymatic Hydrolysis: A Review on Ulvan Lyase

Foods, Journal Year: 2024, Volume and Issue: 13(17), P. 2820 - 2820

Published: Sept. 5, 2024

Ulvan is a water-soluble sulfated polysaccharide extracted from the green algae cell wall. Compared with polysaccharides, oligosaccharides have drawn increasing attention in various industries due to their enhanced biocompatibility and solubility. lyase degrades polysaccharides into low molecular weight through β-elimination mechanism. The elucidation of structure, catalytic mechanism, modification ulvan will be helpful obtain high value-added products marine biomass resources, as well reduce environmental pollution caused by eutrophication algae. This review summarizes structure bioactivity ulvan, microbial origin lyase, its sequence, three-dimensional enzymatic In addition, prospects challenges application methods prepare are also discussed. It provides information for preparation bioactive Ulva hydrolysis, technological bottlenecks, possible solutions address these issues within process.

Language: Английский

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Identification and characterization of a novel thermostable PL7 alginate lyase from a submarine volcanic metagenomic library

Enzyme and Microbial Technology, Journal Year: 2024, Volume and Issue: 180, P. 110486 - 110486

Published: July 21, 2024

Language: Английский

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Chemical and enzymatic hydrolysis of alginate: a review

Botanica Marina, Journal Year: 2024, Volume and Issue: 67(5), P. 487 - 511

Published: Aug. 16, 2024

Abstract Alginate is a brown seaweed-based linear polysaccharide of D – mannuronic acid and L guluronic residues. Hydrolysis products the polysaccharide, specifically oligosaccharides, have been receiving increasing interest, due to their significant bioactivity potential utilisation routes. The alginate oligosaccharides closely linked structural characteristics, namely: molecular weight, degree polymerisation, ratio monomers (the M/G ratio). Hence, applications (such as biostimulant fertilizer) depend on these parameters. This review focuses recent advances in producing using chemical or enzymatic methods. literature survey includes methods at both laboratory industrial scale. For methods, we assessed standard scale procedures oligosaccharide production, scaling up an level, subsequent challenges. route, provide overview lyases application perspectives hydrolysis alginate.

Language: Английский

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Expression and Characterization of an Efficient Alginate Lyase from Psychromonas sp. SP041 through Metagenomics Analysis of Rotten Kelp

Genes, Journal Year: 2024, Volume and Issue: 15(5), P. 598 - 598

Published: May 8, 2024

Alginate is derived from brown algae, which can be cultivated in large quantities. It broken down by alginate lyase into oligosaccharides (AOSs), exhibit a higher added value and better bioactivity than alginate. In this study, metagenomic technology was used to screen for genes that code high-efficiency lyases. The candidate gene alg169 detected Psychromonas sp. SP041, the most abundant species among bacteria on selected rotten kelps. Alg169 heterologously expressed Escherichia coli BL21 (DE3), Ni-IDA-purified, characterized. optimum temperature pH of were 25 °C 7.0, respectively. Metal ions including Mn2+, Co2+, Ca2+, Mg2+, Ni2+, Ba2+ led significantly increased enzyme activity. exhibited pronounced dependence Na+, upon treatment with its activity surged 687.57%, resulting highest observed 117,081 U/mg. Bioinformatic analysis predicted would double-domain molecular weight 65.58 kDa. bifunctional substrate specificity polyguluronic acid (polyG) polymannuronic (polyM). These results suggest promising efficient manufacturing AOSs seaweed.

Language: Английский

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