mSystems,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 20, 2024
A
good
quality
egg
is
essential
for
a
successful
pregnancy
and
early
embryo
development.
Oocyte
development
vulnerable
to
environmental
exposures.
Bisphenol
S
(BPS)
widely
used
as
replacement
its
analog
bisphenol
A,
but
the
reproductive
toxicity
of
BPS
has
been
great
concern.
In
this
study,
we
showed
that
exposure
induces
dysbiosis
gut
microbiota,
which
further
leads
intestinal
permeability
inflammation,
ultimately
impairs
oocyte
quality.
More
importantly,
found
alginate
oligosaccharide
reshapes
microbiota
improve
homeostasis,
thereby
preventing
deleterious
effects
on
oocytes.
Overall,
study
not
only
demonstrates
intestine
oocytes
by
inducing
also
develops
preventive
strategy.
Food & Function,
Journal Year:
2024,
Volume and Issue:
15(19), P. 9632 - 9661
Published: Jan. 1, 2024
Metabolic
syndrome
(MetS)
is
a
disease
condition
incorporating
the
abnormal
accumulation
of
various
metabolic
components,
including
obesity,
insulin
resistance
and
glucose
tolerance,
hypertension,
atherosclerosis,
or
dyslipidemia.
Frontiers in Microbiology,
Journal Year:
2024,
Volume and Issue:
14
Published: Jan. 12, 2024
As
the
major
component
in
cell
wall
of
brown
algae,
alginates
are
degradable
by
alginate
lyases
via
β-elimination.
Alginate
can
be
categorized
into
various
polysaccharide
lyase
(PL)
families,
and
PL7
family
largest
group
divided
six
subfamilies.
However,
difference
among
different
subfamilies
is
not
fully
understood.
In
this
work,
a
marine
lyase,
VaAly2,
from
Vibrio
alginolyticus
ATCC
17749
belonging
to
PL7_5
subfamily
was
identified
characterized.
It
displayed
comparatively
high
alginolytic
activities
toward
substrates
functions
as
bifunctional
lyase.
Molecular
docking
biochemical
analysis
suggested
that
VaAly2
only
contains
key
catalyzing
motif
(HQY)
conserved
but
also
exhibits
some
specific
characters
limited
members,
such
residues
long
loop1
structure
around
active
center.
Our
work
provides
insight
loop
center
site
which
plays
an
important
role
activity
substrate
binding
subfamily.
Journal of Agricultural and Food Chemistry,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 10, 2025
Alginate
is
a
commercially
valuable
polysaccharide
consisting
of
β-d-mannuronate
(M)
and
its
C5
epimer,
α-l-guluronate
(G).
lyases
are
efficient
tools
for
the
degradation
alginate
preparation
oligosaccharides.
In
this
research,
an
endolytic
lyase
Aly7Sa
with
strict
G
specificity
was
expressed
characterized
optimum
reaction
conditions
at
30
°C
pH
6.5.
The
main
products
were
trisaccharide
to
octasaccharide,
those
PolyG
disaccharide
heptasaccharide.
By
utilizing
HPAEC-PAD/MS
NMR
methods,
we
identified
structure
obtained
from
alginate.
Interestingly,
hexasaccharide
contained
only
unsaturated
guluronate
oligosaccharides,
which
different
all
G-specific
lyases.
absence
oligosaccharide
M
residues
demonstrated
Aly7Sa.
targeted
carried
out
based
on
regular
pattern
single-step
purification
employing
gel-permeation
chromatography,
4.8
mg
ΔGG,
6.8
ΔGGG,
3.7
ΔGGGG
100
as
substrate.
strictly
provided
tool
unique
substrate
enzyme
could
also
serve
research
development
