Frontiers in Sustainable Food Systems,
Journal Year:
2024,
Volume and Issue:
7
Published: Feb. 7, 2024
Bone
protein
is
a
significant
secondary
product
of
the
meat
industry,
comprising
substantial
quantity
protein.
These
proteins
could
be
broken
down
through
enzymatic
hydrolysis
to
generate
antioxidant
peptides.
This
study
aimed
produce
peptides
from
bovine
bone
extract
by
utilizing
Flavourzyme
and
Protamex
optimizing
enzyme
amounts
time
using
Box–Behnken
design.
The
final
optimized
conditions
obtained
model
were
as
follows:
amount
was
1,100
U,
2,814
3.77
(h).
Bovine
hydrolysate
(BBEH)
purified
stepwise
ultrafiltration
membranes
with
molecular
cutoffs
5,
3,
1
kDa.
To
assess
capacity
fractions,
several
methods
used,
including
radical
scavenging
activity
“1,1-diphenyl-2-picrylhydrazyl
(DPPH),”
“2,2′-azino-bis
(3-ethylbenzothiazoline-6-sulfonic
acid)
(ABTS),”
metal
chelating
(MCA),
reducing
power
(RP),
thiobarbituric
acid
assay
(TBA).
results
indicated
that
fraction
weight
less
than
kDa
showed
activity,
48,
42,
50%
inhibition
rates
for
DPPH,
ABTS,
chelating,
respectively.
Using
size
exclusion
chromatography,
further
separated
into
five
sub-fractions:
Frac-I,
Frac-II,
Frac-III,
Frac-IV,
Frac-V.
Sub-Frac-III,
which
exhibits
DPPH
(55%)
0.8
at
700
nm,
six
sub-sub-fractions
reversed-phase
HPLC
(RP-HPLC)
based
on
hydrophobicity.
sub-sub-fraction
highest
value
sub-Fra-III-6,
exhibited
approximately
69.45%
activity.
sub-Fra-III-6
analyzed
LC–MS/MS,
identified
two
specific
peptides:
Ala-Pro-Phe
mass
333.12
Da
Asp-His-Val
369.14
Da.
are
likely
primary
might
have
crucial
role
in
capacity.
It
can
concluded
BBEH
valuable
source
natural
antioxidants
has
potential
serve
viable
resource
cultured
industry.
Marine Drugs,
Journal Year:
2023,
Volume and Issue:
21(10), P. 516 - 516
Published: Sept. 28, 2023
The
objective
of
this
study
was
to
isolate
and
characterize
collagen
angiotensin-I-converting
enzyme
(ACE)-inhibitory
(ACEi)
peptides
from
the
swim
bladders
monkfish
(Lophius
litulon).
Therefore,
acid-soluble
(ASC-M)
pepsin-soluble
(PSC-M)
with
yields
4.27
±
0.22%
9.54
0.51%,
respectively,
were
extracted
using
acid
methods.
ASC-M
PSC-M
contained
Gly
(325.2
314.9
residues/1000
residues,
respectively)
as
major
amino
acid,
but
they
had
low
imino
content
(192.5
188.6
in
comparison
calf
skins
(CSC)
(216.6
residues).
sodium
dodecyl
sulfate-polyacrylamide
gel
electrophoresis
(SDS-PAGE)
patterns
ultraviolet
(UV)
absorption
spectrums
illustrated
that
mainly
composed
type
I
collagen.
Subsequently,
three
ACEi
isolated
a
hydrolysate
prepared
via
double-enzyme
system
(alcalase
+
neutrase)
identified
SEGPK
(MHP6),
FDGPY
(MHP7)
SPGPW
(MHP9),
molecular
weights
516.5,
597.6
542.6
Da,
respectively.
SEGPK,
displayed
remarkable
anti-ACE
activity,
IC50
values
0.63,
0.94
0.71
mg/mL,
Additionally,
docking
assay
demonstrated
affinities
ACE
-7.3,
-10.9
-9.4
kcal/mol,
activity
due
their
connection
active
pockets
and/or
sites
hydrogen
bonding,
hydrophobic
interaction
electrostatic
force.
Moreover,
could
protect
HUVECs
by
controlling
levels
nitric
oxide
(NO)
endothelin-1
(ET-1).
work
provides
an
effective
means
for
preparation
collagens
novel
bladders,
peptides,
including
SPGPW,
serve
natural
functional
components
development
health
care
products
control
hypertension.
Antioxidants,
Journal Year:
2024,
Volume and Issue:
13(3), P. 294 - 294
Published: Feb. 28, 2024
Scientific
evidence
attests
that
the
epidermis
receives
excessive
ultraviolet
B
(UVB)
radiation,
triggering
generation
of
substantial
quantities
reactive
oxygen
species
(ROS),
which
disrupted
delicate
equilibrium
oxidation–reduction,
leading
to
oxidative
stress
and
inflammation.
The
historical
use
honeysuckle
polyphenols
(HPs)
has
garnered
our
attention
due
their
efficacy
in
inhibiting
damage.
In
this
study,
HPs
were
prepared
from
flowers
employing
an
ultrasonic-assisted
extraction
method
quantitatively
analyzed
by
a
LC–MS/MS,
mechanisms
underlying
HPs’
antioxidative
anti-inflammatory
effects
on
UVB-irradiated
HaCaT
cell
model
systematically
investigated.
results
showed
had
significant
cellular
repair
effect
cells
(p
<
0.001).
mechanism
action
indicated
could
allow
Nrf2
enter
nucleus
regulating
dissociation
Keap1,
further
increases
activity
downstream
proteases
(SOD
CAT),
ROS
scavenging,
reduces
intracellular
malondialdehyde
(MDA)
level.
addition,
down-regulate
Toll-like
receptor
4
(TLR4)
inhibit
NF-κB
(P65)
dissociating
IκBα,
resulting
decrease
entry
into
inflammatory
factors
(TNF-α,
IL-6,
IL-1β).
four
key
compounds
HPs,
including
chlorogenic
acid,
quercetin,
isorhamnetin,
luteolin,
selected
verify
repairing
UVB
damage
using
molecular
docking
techniques.
experiment
suggested
active
effectively
occupy
Kelch
homologue
(Kelch)
structural
domain
competitively
bind
with
Nrf2,
facilitate
promotion
binding,
ultimately
enhancing
translocation
nucleus.
interact
through
hydrogen
bonding,
van
der
Waals
forces,
electrostatic
forces
its
summary,
can
radiation
be
used
develop
health
cosmetic
products
for
treatment
UV
radiation-induced
diseases.
Marine Drugs,
Journal Year:
2023,
Volume and Issue:
21(11), P. 579 - 579
Published: Nov. 5, 2023
Antarctic
krill
(Euphausia
superba)
is
the
world's
largest
resource
of
animal
proteins
and
thought
to
be
a
high-quality
for
future
marine
healthy
foods
functional
products.
Therefore,
was
degreased
separately
hydrolyzed
using
flavourzyme,
pepsin,
papain,
alcalase.
Protein
hydrolysate
(AKH)
prepared
by
trypsin
showed
highest
Ca-chelating
rate
under
optimized
chelating
conditions:
pH
8.0,
reaction
time
50
min,
temperature
°C,
material/calcium
ratio
1:15.
Subsequently,
fourteen
peptides
were
isolated
from
APK
ultrafiltration
series
chromatographic
methods
identified
as
AK,
EAR,
AEA,
VERG,
VAS,
GPK,
SP,
GPKG,
APRGH,
GVPG,
LEPGP,
LEKGA,
FPPGR,
GEPG
with
molecular
weights
217.27,
374.40,
289.29,
459.50,
275.30,
300.36,
202.21,
357.41,
536.59,
328.37,
511.58,
516.60,
572.66,
358.35
Da,
respectively.
Among
peptides,
VERG
presented
ability.
Ultraviolet
spectrum
(UV),
Fourier
Transform
Infrared
(FTIR),
scanning
electron
microscope
(SEM)
analysis
indicated
that
VERG-Ca
chelate
had
dense
granular
structure
because
N-H,
C=O
-COOH
groups
combined
Ca2+.
Moreover,
stable
in
gastrointestinal
digestion
can
significantly
improve
Ca
transport
Caco-2
cell
monolayer
experiments,
but
phytate
could
reduce
absorption
derived
chelate.
protein
possess
potential
serve
supplement
developing
foods.
