Design-of-Experiment-Guided Establishment of a Fermentative Bioprocess for Biomass-Bound Astaxanthin with Corynebacterium glutamicum DOI Creative Commons
Florian Meyer, Ina Schmitt,

Thomas Schäffer

et al.

Fermentation, Journal Year: 2023, Volume and Issue: 9(11), P. 969 - 969

Published: Nov. 11, 2023

Corynebacterium glutamicum is prominent in the industrial production of secreted amino acids. Notably, it naturally accumulates carotenoid pigment decaprenoxanthin its membranes. Metabolic engineering enabled astaxanthin. Here, a bioprocess for astaxanthin lab-scale stirred bioreactors was established by DoE-guided approach to optimize basic process parameters pH, rDOS, aeration rate as well inoculation cell density. The characterize 2 L scale cultivation revealed that pH showed strongest effect on product formation. Subsequently, an optimum at 8, 0.25 vvm, 30% rDOS and initial optical density 1 allowed 7.6 ± 0.6 mg L−1 batch mode. These conditions were successfully transferred fed-batch resulting high with up 60 g CDW biomass 64 thus demonstrating about 9-fold improvement compared optimal conditions. Moreover, pH-shift experiments indicate cells can quickly adapt change from 6 8 start producing astaxanthin, showing possibility biphasic bioprocesses production.

Language: Английский

Recent Advances in the Therapeutic Potential of Carotenoids in Preventing and Managing Metabolic Disorders DOI Creative Commons
Ana Ortega‐Regules, Juan Alonso Martínez-Thomas,

Karen Schürenkämper-Carrillo

et al.

Plants, Journal Year: 2024, Volume and Issue: 13(12), P. 1584 - 1584

Published: June 7, 2024

Carotenoids constitute compounds of significant biological interest due to their multiple activities, such as antimicrobial, anticancer, antiadipogenic, antidiabetic, and antioxidant properties. Metabolic syndrome (MetS) comprehends a series metabolic abnormalities (e.g., hypertension, obesity, atherogenic dyslipidemia) that can affect children, adolescents, the elderly. The treatment MetS involves numerous medications, which, despite efficacy, pose challenges prolonged use, high costs, various side effects. derivatives have been proposed alternative treatments because they reduce serum triglyceride concentrations, promote insulin response, inhibit adipogenesis, downregulate angiotensin-converting enzyme activity. However, carotenoids are notably sensitive pH, light exposure, temperature. This review addresses activity lycopene, lutein, fucoxanthin, astaxanthin, crocin, β-carotene towards MetS. It includes discussion sources, extraction methods, characterization techniques for analyzing carotenoids. Encapsulation approaches critically reviewed alternatives prevent degradation improve performance A brief overview physiopathology epidemiology diseases, including MetS, is also provided.

Language: Английский

Citations

9
Enhancing astaxanthin biosynthesis and pathway expansion towards glycosylated C40 carotenoids by Corynebacterium glutamicum DOI Creative Commons
Vanessa L. Göttl, Florian Meyer, Ina Schmitt

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: April 6, 2024

Abstract Astaxanthin, a versatile C40 carotenoid prized for its applications in food, cosmetics, and health, is bright red pigment with powerful antioxidant properties. To enhance astaxanthin production Corynebacterium glutamicum , we employed rational pathway engineering strategies, focused on improving precursor availability optimizing terminal oxy-functionalized biosynthesis. Our efforts resulted an increased supply 1.5-fold higher β-carotene strain BETA6 (18 mg g −1 CDW). Further advancements were made by fine-tuning the expression of hydroxylase gene crtZ ketolase crtW yielding nearly fivefold increase (strain ASTA**), constituting 72% total carotenoids. ASTA** was successfully transferred to 2 L fed-batch fermentation enhanced titer 103 volumetric productivity 1.5 h . Based this expansion achieved towards glycosylated carotenoids under heterologous glycosyltransferase crtX best our knowledge, first time astaxanthin-β- d -diglucoside produced C. achieving high titers microbial glucosides 39 This study showcases potential unlock novel variants diverse industrial applications.

Language: Английский

Citations

8
Supercritical carbon dioxide extraction of astaxanthin from Corynebacterium glutamicum DOI
J. Seeger, Maximilian Zäh, Volker F. Wendisch

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: April 4, 2025

Abstract Astaxanthin, a red carotenoid with potent antioxidant properties, holds significant value in the feed, cosmetics, and nutraceutical industries. While traditionally sourced from microalgae, Corynebacterium glutamicum, well-established industrial microorganism, has been engineered to serve as an efficient host for astaxanthin production. As integrates into cellular membrane, effective extraction methods are essential access this valuable compound. In study, sustainable batch process using supercritical carbon dioxide (scCO₂) green solvent was developed. The effects of cosolvent concentration (0–9% (w/w)), temperature (50–75°C), pressure (450–650 bar) were investigated regard yield. An optimized achieved 9% (w/w) ethanol cosolvent, at 68°C 550 bar, allowing 67.5 ± 3.7% within 0.5 hours. Prolonging time further increased recovery 93.3%, which is comparable processes that have established microalgae yeast. This approach provides scalable environmentally friendly solution recovery.

Language: Английский

Citations

0
The Clinical Promise of Microalgae in Rheumatoid Arthritis: From Natural Compounds to Recombinant Therapeutics DOI Creative Commons
Edoardo Andrea Cutolo, Roberto Caferri, Rosanna Campitiello

et al.

Marine Drugs, Journal Year: 2023, Volume and Issue: 21(12), P. 630 - 630

Published: Dec. 7, 2023

Rheumatoid arthritis (RA) is an invalidating chronic autoimmune disorder characterized by joint inflammation and progressive bone damage. Dietary intervention important component in the treatment of RA to mitigate oxidative stress, a major pathogenic driver disease. Alongside traditional sources antioxidants, microalgae-a diverse group photosynthetic prokaryotes eukaryotes-are emerging as anti-inflammatory immunomodulatory food supplements. Several species accumulate therapeutic metabolites-mainly lipids pigments-which interfere pro-inflammatory pathways involved other inflammatory conditions. The advancement clinical uses microalgae requires continuous exploration phytoplankton biodiversity chemodiversity, followed domestication wild strains into reliable producers said metabolites. In addition, tractability microalgal genomes offers unprecedented possibilities establish microbes light-driven biofactories heterologous immunotherapeutics. Here, we review evidence-based mechanisms metabolites provide detailed coverage genetic engineering strategies enhance yields endogenous compounds develop innovative bioproducts.

Language: Английский

Citations

6
Design-of-Experiment-Guided Establishment of a Fermentative Bioprocess for Biomass-Bound Astaxanthin with Corynebacterium glutamicum DOI Creative Commons
Florian Meyer, Ina Schmitt,

Thomas Schäffer

et al.

Fermentation, Journal Year: 2023, Volume and Issue: 9(11), P. 969 - 969

Published: Nov. 11, 2023

Corynebacterium glutamicum is prominent in the industrial production of secreted amino acids. Notably, it naturally accumulates carotenoid pigment decaprenoxanthin its membranes. Metabolic engineering enabled astaxanthin. Here, a bioprocess for astaxanthin lab-scale stirred bioreactors was established by DoE-guided approach to optimize basic process parameters pH, rDOS, aeration rate as well inoculation cell density. The characterize 2 L scale cultivation revealed that pH showed strongest effect on product formation. Subsequently, an optimum at 8, 0.25 vvm, 30% rDOS and initial optical density 1 allowed 7.6 ± 0.6 mg L−1 batch mode. These conditions were successfully transferred fed-batch resulting high with up 60 g CDW biomass 64 thus demonstrating about 9-fold improvement compared optimal conditions. Moreover, pH-shift experiments indicate cells can quickly adapt change from 6 8 start producing astaxanthin, showing possibility biphasic bioprocesses production.

Language: Английский

Citations

2