Journal of Water Process Engineering, Journal Year: 2025, Volume and Issue: 73, P. 107692 - 107692
Published: April 14, 2025
Language: Английский
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 16, 2025
The complement cascade is a front-line defense against pathogens. Complement activation generates the membrane attack complex (MAC), 10-11 nm diameter pore formed by proteins C5b through C8 and polymerized C9. MAC embeds within outer of Gram-negative bacteria displays bactericidal activity. In absence C9, C5b-C8 complexes can form 2-4 pores on membranes, but their relevance to microbial control poorly understood. Deficiencies in terminal components uniquely predispose individuals infections pathogenic Neisseria, including N. gonorrhoeae (Gc). Increasing antibiotic resistance Gc makes new therapeutic strategies priority. Here, we demonstrate that activity human serum disrupts inner potentiating antimicrobials re-sensitizing multidrug resistant antibiotics. C9-depleted also membranes exerts antigonococcal activity, effects are not reported other bacteria. formation potentiates sensitivity azithromycin lysozyme. These findings expand our mechanistic understanding lytic suggest size limitation for complement-mediated enhancement Gc, manipulation be used combat drug-resistant gonorrhea. arm innate immune system results (MAC) forming bacteria, resulting bacterial death. Individuals who cannot generate specifically susceptible infection Neisseria species High rates gonorrhea its complications like infertility, high-frequency multiple antibiotics, make it important identify approaches Gc. Beyond direct anti-Gc found increases ability antibiotics antimicrobial kill re-sensitizes multidrug-resistant most component, needed potentiate lysozyme, potentiated regardless highlight unique novel translational avenues
Language: Английский
Citations
0
Cytology and Genetics, Journal Year: 2025, Volume and Issue: 59(1), P. 1 - 10
Published: Feb. 1, 2025
Language: Английский
Citations
0
Pharmaceuticals, Journal Year: 2025, Volume and Issue: 18(3), P. 402 - 402
Published: March 12, 2025
The escalating global health crisis of antibiotic resistance, driven by the rapid emergence multidrug-resistant (MDR) bacterial pathogens, necessitates urgent and innovative countermeasures. This review comprehensively examines diverse mechanisms employed bacteria to evade action, including alterations in cell membrane permeability, efflux pump overexpression, biofilm formation, target site modifications, enzymatic degradation antibiotics. Specific focus is given transport systems such as ATP-binding cassette (ABC) transporters, resistance–nodulation–division (RND) pumps, major facilitator superfamily (MFS) multidrug toxic compound extrusion (MATE) systems, small resistance (SMR) families, proteobacterial antimicrobial (PACE) families. Additionally, explores burden MDR pathogens evaluates emerging therapeutic strategies, quorum quenching (QQ), probiotics, postbiotics, synbiotics, peptides (AMPs), stem applications, immunotherapy, antibacterial photodynamic therapy (aPDT), bacteriophage. Furthermore, this discusses novel agents, animal-venom-derived compounds nanobiotics, promising alternatives conventional interplay between clustered regularly interspaced short palindromic repeats (CRISPR) CRISPR-associated proteins (Cas) adaptive immunity analyzed, revealing opportunities for targeted genetic interventions. By synthesizing current advancements underscores necessity interdisciplinary collaboration among biomedical scientists, researchers, pharmaceutical industry drive development agents. Ultimately, comprehensive analysis provides a roadmap future research, emphasizing need sustainable cooperative approaches combat safeguard health.
Language: Английский
Citations
0
mBio, Journal Year: 2025, Volume and Issue: unknown
Published: March 31, 2025
ABSTRACT The complement cascade is a front-line defense against pathogens. Complement activation generates the membrane attack complex (MAC), 10–11 nm diameter pore formed by proteins C5b through C8 and polymerized C9. MAC embeds within outer of Gram-negative bacteria displays bactericidal activity. In absence C9, C5b-C8 complexes can form 2–4 pores on membranes, but their relevance to microbial control poorly understood. Deficiencies in terminal components uniquely predispose individuals infections pathogenic Neisseria , including N. gonorrhoeae (Gc). Increasing antibiotic resistance Gc makes new therapeutic strategies priority. Here, we demonstrate that activity human serum disrupts inner potentiating antimicrobials re-sensitizing multidrug-resistant antibiotics. C9-depleted also exerts and, unlike other bacteria, both membranes. formation potentiates sensitivity azithromycin ceftriaxone, not lysozyme or nisin. These findings expand our mechanistic understanding lytic activity, suggest size limitation for complement-mediated enhancement Gc, manipulation be used combat drug-resistant gonorrhea. IMPORTANCE arm innate immune system results (MAC) forming resulting bacterial death. Individuals who cannot generate are specifically susceptible infection species High rates gonorrhea, its complications like infertility, high-frequency multiple antibiotics make it important identify approaches Gc. Beyond direct anti-Gc found increases ability antimicrobial kill re-sensitizes most component, needed potentiate nisin, ceftriaxone potentiated regardless highlight unique effects novel translational avenues
Language: Английский
Citations
0
Journal of Water Process Engineering, Journal Year: 2025, Volume and Issue: 73, P. 107692 - 107692
Published: April 14, 2025
Language: Английский
Citations
0