Mass Spectrometry Advancements and Applications for Biomarker Discovery, Diagnostic Innovations, and Personalized Medicine

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(18), P. 9880 - 9880

Published: Sept. 12, 2024

Mass spectrometry (MS) has revolutionized clinical chemistry, offering unparalleled capabilities for biomolecule analysis. This review explores the growing significance of mass (MS), particularly when coupled with liquid chromatography (LC), in identifying disease biomarkers and quantifying biomolecules diagnostic prognostic purposes. The unique advantages MS accurately diverse molecules have positioned it as a cornerstone personalized-medicine advancement. MS-based technologies transformed precision medicine, enabling comprehensive understanding mechanisms patient-specific treatment responses. LC-MS shown exceptional utility analyzing complex biological matrices, while high-resolution expanded analytical capabilities, allowing detection low-abundance elucidation pathways. integration other techniques, such ion mobility spectrometry, opened new avenues biomarker discovery validation. As we progress toward will be crucial addressing challenges individualized patient care, driving innovations diagnosis, prognosis, strategies.

Language: Английский

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Advancing Clinical Liquid Chromatography-Mass Spectrometry Analysis: Rational Design and Optimization of Derivatization Techniques for Enhanced Sensitivity and Throughput

Journal of Chromatography Open, Journal Year: 2025, Volume and Issue: unknown, P. 100215 - 100215

Published: March 1, 2025

Language: Английский

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The salivary metabolomics analyses reveal the variable metabolites in distinct staging of periodontitis

BMC Oral Health, Journal Year: 2025, Volume and Issue: 25(1)

Published: April 3, 2025

Saliva, which is a critical component of the oral ecosystem, undergoes dynamic changes, particularly during onset and progression periodontitis. This study used Gas Chromatography-Mass Spectrometry (GC-MS), reliable high-throughput tool for metabolomic analysis, to detect salivary metabolic shifts across various stages periodontitis (T1-T4). We compared differential changes in metabolites between HC T1 groups, T2 T3 T4 groups. By analysing saliva samples from 116 individuals-10 healthy controls (HC) 106 patients with (22 individuals) (28 individuals), we identified including Glucose, 3-Aminobutanoic Acid, N-(1-Cyclopropylethyl) Aniline, Methylmalonic Acid. Compared HC, exhibited progressive concentration correlating severity disease. Furthermore, KEGG pathway analysis was elucidate pathways involved development Our findings demonstrate potential as biomarkers monitoring offer valuable insights into its pathogenesis therapeutic targets.

Language: Английский

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Advancing clinical biochemistry: addressing gaps and driving future innovations

Frontiers in Medicine, Journal Year: 2025, Volume and Issue: 12

Published: April 8, 2025

Modern healthcare depends fundamentally on clinical biochemistry for disease diagnosis and therapeutic guidance. The discipline encounters operational constraints, including sampling inefficiencies, precision limitations, expansion difficulties. Recent advancements in established technologies, such as mass spectrometry the development of high-throughput screening point-of-care are revolutionizing industry. biosensor technology wearable monitors facilitate continuous health tracking, Artificial Intelligence (AI)/machine learning (ML) applications enhance analytical capabilities, generating predictive insights individualized treatment protocols. However, concerns regarding algorithmic bias, data privacy, lack transparency decision-making ("black box" models), over-reliance automated systems pose significant challenges that must be addressed responsible AI integration. limitations remain-substantial implementation expenses, system incompatibility issues, information security vulnerabilities intersect with ethical considerations fairness protected information. Addressing these demands coordinated efforts between clinicians, scientists, technical specialists. This review discusses current biochemistry, explicitly addressing reference intervals barriers to implementing innovative biomarkers medical settings. discussion evaluates how advanced technologies multidisciplinary collaboration can overcome constraints while identifying research priorities diagnostic accessibility better delivery.

Language: Английский

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Metabolism of sphingolipids in a rat spinal cord stenosis model

Biochemistry and Biophysics Reports, Journal Year: 2025, Volume and Issue: 42, P. 102025 - 102025

Published: April 26, 2025

Language: Английский

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Biophysical characterization of hydrogen sulfide: A fundamental exploration in understanding significance in cell signaling

Biophysical Chemistry, Journal Year: 2024, Volume and Issue: 314, P. 107317 - 107317

Published: Aug. 30, 2024

Language: Английский

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Overview of Bovine Mastitis: Application of Metabolomics in Screening Its Predictive and Diagnostic Biomarkers

Animals, Journal Year: 2024, Volume and Issue: 14(15), P. 2264 - 2264

Published: Aug. 4, 2024

Bovine mastitis is an inflammatory disease of the mammary glands, and its pathogenesis diagnosis are complicated. Through qualitative quantitative analysis small-molecule metabolites, metabolomics technique plays important role in finding biomarkers studying metabolic mechanism bovine mastitis. Therefore, this paper reviews predictive diagnostic that have been identified using techniques present samples such as milk, blood, urine, rumen fluid, feces, tissue. In addition, pathways mastitis-related milk blood were analyzed; it was found tricarboxylic acid (TCA) cycle most significant (FDR = 0.0015767) pathway glyoxylate dicarboxylate metabolism 0.0081994) blood. The purpose review to provide useful information for prediction early

Language: Английский

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Effect of a Phytochemical-Rich Olive-Derived Extract on Anthropometric, Hematological, and Metabolic Parameters

Nutrients, Journal Year: 2024, Volume and Issue: 16(18), P. 3068 - 3068

Published: Sept. 11, 2024

Background: Extra virgin olive oil is a fundamental component of the Mediterranean diet. It contains several molecules that sustain human well-being by modulating cellular metabolism and exerting antioxidant, anti-inflammatory, anti-ageing effects to protect normal tissues, it can exert anti-angiogenic pro-apoptotic on cancer cells. Metabolites found in different parts tree, including leaves, also possess properties might help prevention promote wellness aging. Olive mill wastewater (OMWW), liquid residue produced during extraction, represents an environmental issue. However, rich phytochemicals with potential beneficial properties. Dietary supplements based OMWW be for nutritional supplementation advantages ecology. Purpose: This work aims measure hematochemical, anthropometric, metabolomic parameters volunteers taking dietary supplement, Oliphenolia® (OMWW-OL). Methods: The OMWW-OL 25 mL twice daily 30 days was tested pilot cohort characteristics close metabolic syndrome. Hematochemical, serum biomarkers were analyzed before intervention, at days, after stopping consumption. Results: A total 29 enrolled, 23 completed study. participants’ baseline measured, then treatment assumption discontinuation. Although derivative, their weight did not increase. Their body mass index, instead augmenting, slightly decreased, particularly women. Also, hydration increased, especially women, while blood pressure, glycemia, insulin decreased. Cholesterol, high-density lipoproteins, triglycerides stable, LDL levels vitamin D levels, alongside calcium, perceptibly increased. Albumin All values support equilibrium, no damaging effects. By spectrometry analysis, we favorable changes D/histamine homocysteine/methionine ratios, increase new metabolite unknown formula, D/unknown ratio. Conclusions: Supplementation has detrimental imply modulation biological parameters. this small study, limited potency, preliminarily suggests extract use could potentially valuable people risk Some these relevant supporting healthy ageing prevention.

Language: Английский

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Struggling to Understand the NEC Spectrum—Could the Integration of Metabolomics, Clinical-Laboratory Data, and Other Emerging Technologies Help Diagnosis?

Metabolites, Journal Year: 2024, Volume and Issue: 14(10), P. 521 - 521

Published: Sept. 26, 2024

Necrotizing enterocolitis (NEC) is the most prevalent and potentially fatal intestinal injury mainly affecting premature infants, with significant long-term consequences for those who survive. This review explores scale of problem, highlighting advancements in epidemiology, understanding pathophysiology, improvements prediction diagnosis this complex, multifactorial, multifaced disease. Additionally, we focus on potential role metabolomics distinguishing NEC from other conditions, which could allow an earlier more accurate classification injuries infants. By integrating metabolomic data diagnostic approaches, it hoped to enhance our ability predict outcomes tailor treatments, ultimately improving care affected

Language: Английский

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Research Progress of Metabolomics in Idiopathic Pulmonary Interstitial Fibrosis

Journal of Clinical Personalized Medicine, Journal Year: 2024, Volume and Issue: 03(01), P. 68 - 73

Published: Jan. 1, 2024

Language: Английский

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