Classical and emerging approximations for the screening of antimicrobial peptide libraries
Elsevier eBooks,
Journal Year:
2025,
Volume and Issue:
unknown, P. 195 - 232
Published: Jan. 1, 2025
Language: Английский
Nanotheranostics Revolutionizing Gene Therapy: Emerging Applications in Gene Delivery Enhancement
Journal of Nanotheranostics,
Journal Year:
2025,
Volume and Issue:
6(2), P. 10 - 10
Published: April 9, 2025
Nanotheranostics—where
nanoscale
materials
serve
both
diagnostic
and
therapeutic
functions—are
rapidly
transforming
gene
therapy
by
tackling
critical
delivery
challenges.
This
review
explores
the
design
engineering
of
various
nanoparticle
systems
(lipid-based,
polymeric,
inorganic,
hybrid)
to
enhance
stability,
targeting,
endosomal
escape
genetic
payloads.
We
discuss
how
real-time
imaging
capabilities
integrated
into
these
platforms
enable
precise
localization
controlled
release
genes,
improving
treatment
efficacy
while
reducing
off-target
effects.
Key
strategies
overcome
barriers
(such
as
proton
sponge
effect
photothermal
disruption)
achieve
nuclear
are
highlighted,
along
with
recent
advances
in
stimuli-responsive
that
facilitate
spatiotemporal
control
expression.
Clinical
trials
preclinical
studies
demonstrate
expanding
role
nanotheranostics
managing
cancer,
inherited
disorders,
cardiovascular
neurological
diseases.
further
address
regulatory
manufacturing
hurdles
must
be
for
widespread
clinical
adoption
nanoparticle-based
therapies.
By
synthesizing
progress
ongoing
challenges,
this
underscores
transformative
potential
effective,
targeted,
image-guided
delivery.
Language: Английский
Design, Characterization, and Evaluation of Textile Systems and Coatings for Sports Use: Applications in the Design of High-Thermal Comfort Wearables
ACS Omega,
Journal Year:
2024,
Volume and Issue:
9(50), P. 49143 - 49162
Published: Nov. 28, 2024
Exposure
to
high
temperatures
during
indoor
and
outdoor
activities
increases
the
risk
of
heat-related
illness
such
as
cramps,
rashes,
heatstroke
(HS).
Fatal
cases
HS
are
ten
times
more
common
than
serious
cardiac
episodes
in
sporting
scenarios,
with
untreated
leading
mortality
rates
80%.
Enhancing
thermal
comfort
can
be
achieved
through
heat
loss
enclosed
spaces
human
body,
utilizing
transfer
mechanisms
radiation,
conduction,
convection,
evaporation,
which
do
not
require
initial
energy
input.
Among
these,
two
primary
commonly
employed
textile
industry
enhance
passive
cooling:
radiation
conduction.
The
approach
encompasses
aspects:
(1)
reflecting
solar
spectrum
(SS)
wavelengths
(2)
transmitting
emitting
atmospheric
window
(AW).
Conduction
involves
dissipating
materials
a
conductivity.
Our
study
focuses
on
combined
effect
these
radiative
conductive
approaches
increase
loss,
an
area
that
has
been
extensively
studied
date.
Therefore,
main
objective
this
project
is
develop,
characterize,
evaluate
nanocomposite
polymeric
system
using
electrospinning,
incorporating
graphene
oxide
(GO)
nanosheets
titanium
dioxide
nanoparticles
(TiO
Language: Английский
Production and purification of outer membrane vesicles encapsulating green fluorescent protein from Escherichia coli: a step towards scalable OMV technologies
Frontiers in Bioengineering and Biotechnology,
Journal Year:
2024,
Volume and Issue:
12
Published: Nov. 14, 2024
Outer
membrane
vesicles
(OMVs)
are
spherical
structures
that
contain
a
small
fraction
of
the
periplasm
Gram-negative
bacteria,
surrounded
by
its
outer
membrane.
They
naturally
produced
and
detached
from
bacterial
surface,
participate
in
diverse
biological
processes,
their
diameter
size
is
range
10–300
nm.
OMVs
have
gained
interest
different
applications,
such
as
development
biosensors,
vaccines,
protein
chips,
encapsulation
heterologous
proteins
peptides
expressed
these
microorganisms.
However,
use
applications
limited
due
to
low
yields
high
purification
costs.
In
this
study,
we
green
fluorescent
(GFP)
encapsulated
into
using
Escherichia
coli
JC8031
transformed
with
pTRC99A-ssTorA-GFP
establish
production
route.
Results
showed
motility
strain
prevents
immobilization
alginate,
which
hampers
OMVs.
To
address
issue,
zeolite-based
column
was
used
chromatographically
separate
smaller
particles.
Further
experiments
will
be
focused
on
standardizing
at
scalable
level.
Language: Английский