AMB Express,
Journal Year:
2025,
Volume and Issue:
15(1)
Published: Jan. 29, 2025
Candida
albicans
is
a
commensal
fungus
that
naturally
inhabits
the
vagina.
However,
overgrowth
of
C.
can
result
in
vulvovaginal
candidiasis
(VVC),
one
most
prevalent
fungal
infections
affecting
women.
The
rapid
emergence
azole
resistance
albicans,
addition
to
limited
available
antifungal
agents,
complicates
treatment
and
emphasizes
urgent
need
for
novel
therapeutic
options.
Efflux-mediated
common
mechanism
fluconazole
(FLZ)-resistant
albicans.
Combination
therapy
using
natural
compounds
potential
approach
restore
fluconazole's
activity
azole-resistant
isolates
via
efflux
pump
inhibition.
This
study
aimed
evaluate
ability
celastrol,
triterpene,
retrieve
FLZ
against
vitro
vivo.
Celastrol
did
not
exhibit
tested
clinical
isolates;
however,
sub-MIC
celastrol
inhibited
rhodamine
6G
(R6G)
increased
R6G
accumulation
inside
celastrol-treated
cells.
Synergy
was
spotted
between
checkerboard
assay.
Quantification
m-RNA
levels
efflux-mediated
genes
within
demonstrated
CDR1
overexpression.
Upon
treatment,
significant
decline
ABC
transporters
transcript
were
detected.
Moreover,
molecular
docking
blocker
successfully
fits
into
target
binding
pockets.
A
negligible
hemolytic
effect
human
erythrocytes
observed.
In
vivo
model
VVC,
combination
vaginal
gel
revealed
drastic
reduction
burden
with
apparently
normal
tissue.
promising
findings
strengthen
its
future
use
AMB Express,
Journal Year:
2024,
Volume and Issue:
14(1)
Published: Aug. 1, 2024
Abstract
Resistance
to
antibiotics
is
a
critical
growing
public
health
problem
that
desires
urgent
action
combat.
To
avoid
the
stress
on
bacterial
growth
evokes
resistance
development,
anti-virulence
agents
can
be
an
attractive
strategy
as
they
do
not
target
growth.
Quorum
sensing
(QS)
systems
play
main
roles
in
controlling
production
of
diverse
virulence
factors
and
biofilm
formation
bacteria.
Thus,
interfering
with
QS
could
result
mitigation
virulence.
Cilostazol
antiplatelet
vasodilator
FDA
approved
drug.
This
study
aimed
evaluate
activities
cilostazol
light
its
possible
interference
Pseudomonas
aeruginosa
.
Additionally,
examines
cilostazol’s
impact
bacterium’s
ability
induce
infection
vivo,
using
sub-inhibitory
concentrations
minimize
risk
development.
In
this
context,
formation,
influence
vivo
were
assessed
presence
at
concentration.
Furthermore,
outcome
combination
was
evaluated.
interfered
P.
Moreover,
swarming
motility,
significantly
diminished.
Histopathological
investigation
revealed
liver,
spleen
kidney
tissues
damage
abolished
mice
injected
cilostazol-treated
exhibited
synergistic
when
used
antibiotics.
At
molecular
level,
downregulated
genes
showed
considerable
affinity
receptors.
conclusion,
adjunct
therapy
for
treating
Pseudomonal
infections.
research
highlights
potential
combat
infections
by
targeting
mechanisms,
reducing
antibiotic
resistance,
enhancing
treatment
efficacy
against
These
findings
open
avenues
repurposing
existing
drugs,
offering
new,
safer,
more
effective
control
strategies.
ACS Omega,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 16, 2025
Sulforaphane
is
a
main
bioactive
component
in
several
edible
cruciferous
vegetables.
It
acquires
benefits
to
health
addition
its
considered
antibacterial
and
antivirulence
activities.
Herein,
we
aimed
at
evaluating
the
activity
of
sulforaphane
against
worldwide
clinically
important
enteric
pathogen
Salmonella
enterica
serovar
Typhimurium.
The
influence
on
bacterial
adhesion,
invasion,
biofilm
formation,
intracellular
replication
was
assayed.
Additionally,
effect
type
III
secretion
system
(TTSS)
S.
quantified.
outcome
combination
with
different
antibiotics
assessed,
an
vivo
protection
assay
conducted
assess
pathogenesis.
results
showed
significant
antibiofilm
subinhibitory
reduction
invasion
inside
host
cells.
findings
emphasized
decreased
capacity
induce
pathogenesis
presence
sulforaphane.
Our
finding
attributed
these
activities
interference
TTSS-type
II
downregulation
encoding
genes.
In
nutshell,
vegetable
safe
adjunct
therapy
that
can
be
administrated
alongside
traditional
for
treating
pathogens
as
enterica.
AMB Express,
Journal Year:
2025,
Volume and Issue:
15(1)
Published: Jan. 29, 2025
Candida
albicans
is
a
commensal
fungus
that
naturally
inhabits
the
vagina.
However,
overgrowth
of
C.
can
result
in
vulvovaginal
candidiasis
(VVC),
one
most
prevalent
fungal
infections
affecting
women.
The
rapid
emergence
azole
resistance
albicans,
addition
to
limited
available
antifungal
agents,
complicates
treatment
and
emphasizes
urgent
need
for
novel
therapeutic
options.
Efflux-mediated
common
mechanism
fluconazole
(FLZ)-resistant
albicans.
Combination
therapy
using
natural
compounds
potential
approach
restore
fluconazole's
activity
azole-resistant
isolates
via
efflux
pump
inhibition.
This
study
aimed
evaluate
ability
celastrol,
triterpene,
retrieve
FLZ
against
vitro
vivo.
Celastrol
did
not
exhibit
tested
clinical
isolates;
however,
sub-MIC
celastrol
inhibited
rhodamine
6G
(R6G)
increased
R6G
accumulation
inside
celastrol-treated
cells.
Synergy
was
spotted
between
checkerboard
assay.
Quantification
m-RNA
levels
efflux-mediated
genes
within
demonstrated
CDR1
overexpression.
Upon
treatment,
significant
decline
ABC
transporters
transcript
were
detected.
Moreover,
molecular
docking
blocker
successfully
fits
into
target
binding
pockets.
A
negligible
hemolytic
effect
human
erythrocytes
observed.
In
vivo
model
VVC,
combination
vaginal
gel
revealed
drastic
reduction
burden
with
apparently
normal
tissue.
promising
findings
strengthen
its
future
use
